Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,902,495 A→C 12.6% G200G (GGA→GGC) ‡ ygcE → putative kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,902,4950AC12.6% 254.3 / 25.4 102G200G (GGA→GGC) ‡ygcEputative kinase
Reads supporting (aligned to +/- strand):  ref base A (39/50);  new base C (7/6);  total (46/56)
Fisher's exact test for biased strand distribution p-value = 5.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

Only 100 of 102 total aligned reads displayed.

ATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACT  >  NC_000913/2902347‑2902643
                                                                                                                                                    |                                                                                                                                                    
aTTATACGAGCTGGCCATCGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATAAGCTGTTTGATCTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGAc                                                                                                                                                     <  1:285311/150‑1 (MQ=255)
 ttATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACt                                                                                                                                                    <  2:247771/150‑1 (MQ=255)
    taAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGcc                                                                                                                                                 <  1:226055/150‑1 (MQ=255)
    taAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGcc                                                                                                                                                 >  1:1520942/1‑150 (MQ=255)
    taAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGcc                                                                                                                                                 <  2:162601/150‑1 (MQ=255)
      agagCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGTCCTGCCgg                                                                                                                                               >  2:1260707/1‑150 (MQ=255)
      agagCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGTCCTGCCgg                                                                                                                                               >  2:1254103/1‑150 (MQ=255)
       gagCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGgt                                                                                                                                              >  2:1248268/1‑150 (MQ=255)
        agCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGTCCTGCCggga                                                                                                                                             <  2:1417935/150‑3 (MQ=255)
          cTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGTCCTGCCgggaaa                                                                                                                                           <  2:512280/150‑5 (MQ=255)
          cTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGTCCTGCCgggaaa                                                                                                                                           <  1:573613/150‑5 (MQ=255)
           tgtgCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCTTATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCAAACCACTAGCCGCTCTGGCGACACATTTCCCGGCAGGACTGCCGgttgtt                                                                                                                                          <  1:1135782/147‑1 (MQ=255)
           tGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGTCCTGCCgggaaat                                                                                                                                          <  1:1254103/150‑6 (MQ=255)
           tGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGTCCTGCCgggaaat                                                                                                                                          <  1:1260707/150‑6 (MQ=255)
            gggCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGttgttt                                                                                                                                         <  2:547870/150‑1 (MQ=255)
               cATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTa                                                                                                                                      >  1:867071/1‑150 (MQ=255)
                aTGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTac                                                                                                                                     >  1:624311/1‑150 (MQ=255)
                 tGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTacc                                                                                                                                    >  1:356645/1‑150 (MQ=255)
                 tGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCACCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTacc                                                                                                                                    >  1:348884/1‑150 (MQ=255)
                    aGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTaccacc                                                                                                                                 <  1:321055/150‑1 (MQ=255)
                     gCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTAccacca                                                                                                                                <  2:1395692/150‑1 (MQ=255)
                       taaGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACTACCAGt                                                                                                                              <  1:357745/149‑1 (MQ=255)
                            tgcttcGGTAATTGATAAGTTTTATTTCCCCCGTCATATGCTGTTTGCTGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGACGCACTGGCGACAGATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATaa                                                                                                                         <  1:1305654/150‑1 (MQ=255)
                              ctgcGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAAc                                                                                                                       >  2:1697913/1‑150 (MQ=255)
                               tgcGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAAcc                                                                                                                      >  1:1381288/1‑150 (MQ=255)
                                gcGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCg                                                                                                                     <  1:1532390/150‑1 (MQ=255)
                                 cGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCgg                                                                                                                    <  1:940116/150‑1 (MQ=255)
                                  ggTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGt                                                                                                                   <  1:1167537/150‑1 (MQ=255)
                                  ggTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGt                                                                                                                   <  1:933753/150‑1 (MQ=255)
                                   gTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTa                                                                                                                  >  2:1432140/1‑150 (MQ=255)
                                    tAATTGATAAGTTTAATATCCCACGTCATATGCTTTTTGATGTGCAAATGCCTGTCACCGTCCTCGGACATATAACACCACAAGCCGAACTGGCGACACATTTCCCGGCAGGACTGCATGTTGTTTGTACCACCAGTGATAAACCGGTAg                                                                                                                 <  1:429925/150‑1 (MQ=255)
                                        ggATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGc                                                                                                             <  1:716930/150‑1 (MQ=255)
                                        ggATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGc                                                                                                             >  1:968044/1‑150 (MQ=255)
                                          aTAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGctc                                                                                                           >  1:1279228/1‑150 (MQ=255)
                                             acgatAATATCCCGCGTCATATCCTCATTGATGTGCAAATGCCTCGCACCGACCTCGGACATATCACACCACAAGCCGGACTCGCGACTCATTACCCGGCAGGACTGCCGGTTGTTTGTAGGACCAGTGATAAACCGGTAGAATCTCTgg                                                                                                        <  2:1610236/146‑1 (MQ=255)
                                             aGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTgg                                                                                                        >  2:579104/1‑150 (MQ=255)
                                             aGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTgg                                                                                                        >  1:925176/1‑150 (MQ=255)
                                             aGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTgg                                                                                                        >  2:1436620/1‑150 (MQ=255)
                                               tttAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTgggg                                                                                                      <  2:1087775/150‑1 (MQ=255)
                                                 tAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGc                                                                                                    >  1:1164504/1‑150 (MQ=255)
                                                  aaTATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGcc                                                                                                   <  1:579312/150‑1 (MQ=255)
                                                  aaTATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGcc                                                                                                   <  2:1164504/150‑1 (MQ=255)
                                                  aaTATCCCCCGTCATATGCTGTGTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACACGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGcc                                                                                                   <  1:1655780/150‑1 (MQ=255)
                                                   atatCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCg                                                                                                  >  2:1507985/1‑150 (MQ=255)
                                                   atatCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTATTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCg                                                                                                  <  2:1487606/150‑1 (MQ=255)
                                                    tatCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCgg                                                                                                 <  1:1717761/150‑1 (MQ=255)
                                                      tCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGAt                                                                                               <  1:1248268/150‑1 (MQ=255)
                                                      tCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGGCCTCGGACATATCACCCCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGAt                                                                                               <  1:699226/150‑1 (MQ=255)
                                                       cccccGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGAtt                                                                                              <  1:1028509/150‑1 (MQ=255)
                                                        ccccGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTa                                                                                             >  1:1156813/1‑150 (MQ=255)
                                                          ccGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGCCGACACAATTCCCGGCAGCAATGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTGTGGGGGCCGGATTACt                                                                                           >  1:173695/1‑150 (MQ=255)
                                                           cGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTg                                                                                          <  1:1507985/150‑1 (MQ=255)
                                                             tCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACTCCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGga                                                                                        <  2:162273/150‑1 (MQ=255)
                                                              aatatGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGgat                                                                                       <  2:862988/149‑1 (MQ=255)
                                                               atatGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGgatg                                                                                      <  2:1520942/150‑1 (MQ=255)
                                                               atatGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGgatg                                                                                      <  1:59062/150‑1 (MQ=255)
                                                                tatGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGCTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGgatga                                                                                     >  1:1466903/1‑150 (MQ=255)
                                                                   gCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGAtgatga                                                                                  >  2:1016763/1‑150 (MQ=255)
                                                                      gTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAAc                                                                               >  1:666602/1‑150 (MQ=255)
                                                                          gATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAAcggcg                                                                           <  2:968044/150‑1 (MQ=255)
                                                                              tgCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTaa                                                                       <  2:1078939/150‑1 (MQ=255)
                                                                              tgCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTaa                                                                       >  1:191300/1‑150 (MQ=255)
                                                                                      ccTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGCCACATTTCCCGGCAGGACTGCCGGATGTTTGTACCACCAGTGAAAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGAAATTTCTCtt                                                               >  1:74248/1‑150 (MQ=255)
                                                                                       cTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTg                                                              <  2:1381288/150‑1 (MQ=255)
                                                                                                  cTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATc                                                   >  2:635009/1‑150 (MQ=255)
                                                                                                   tCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCg                                                  <  1:635009/150‑1 (MQ=255)
                                                                                                   tCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCg                                                  <  2:1580543/150‑1 (MQ=255)
                                                                                                     ggACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATATACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCa                                                >  2:913706/1‑150 (MQ=255)
                                                                                                         atacCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATtga                                            <  1:732114/150‑1 (MQ=255)
                                                                                                          tatCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATtgat                                           >  1:1012546/1‑150 (MQ=255)
                                                                                                          tatCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATtgat                                           >  1:193330/1‑150 (MQ=255)
                                                                                                          tatCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATtgat                                           >  1:1352792/1‑150 (MQ=255)
                                                                                                              acacCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATtgatgatg                                       >  2:768122/1‑150 (MQ=255)
                                                                                                                 ccaccaGCCGCACTGGCGACACAATTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGAAGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAAc                                    <  2:128042/150‑1 (MQ=255)
                                                                                                                 ccacAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAAc                                    >  1:371923/1‑150 (MQ=255)
                                                                                                                  cacaAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACg                                   <  2:1156813/150‑1 (MQ=255)
                                                                                                                   acaAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACgg                                  <  2:348884/150‑1 (MQ=255)
                                                                                                                    caAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGc                                 <  2:666602/150‑1 (MQ=255)
                                                                                                                      aGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCaa                               >  2:1500954/1‑150 (MQ=255)
                                                                                                                       gCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCaaa                              >  1:356861/1‑150 (MQ=255)
                                                                                                                       gCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCaaa                              >  1:704592/1‑150 (MQ=255)
                                                                                                                         cGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGc                            <  2:191300/150‑1 (MQ=255)
                                                                                                                         cGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCAGTGATGATGAACGGCAAAGc                            >  2:1378800/1‑150 (MQ=255)
                                                                                                                               ggCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGcc                      >  2:1238994/1‑150 (MQ=255)
                                                                                                                               ggCGACACATTTCCCGGCAGGACTGACGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGcc                      >  2:279345/1‑150 (MQ=255)
                                                                                                                                gCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCg                     >  2:1167985/1‑150 (MQ=255)
                                                                                                                                 cGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGa                    <  2:1279228/150‑1 (MQ=255)
                                                                                                                                     caaacaaccGGCAGTCCTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGa                >  1:377704/8‑150 (MQ=255)
                                                                                                                                       aTTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATc              >  2:371358/1‑150 (MQ=255)
                                                                                                                                        tttCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATcc             <  1:995662/150‑1 (MQ=255)
                                                                                                                                        tttCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATcc             <  1:1167985/150‑1 (MQ=255)
                                                                                                                                        tttCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATcc             <  1:1697913/150‑1 (MQ=255)
                                                                                                                                         caaccGGCAGTCCTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGCAGCGCTCGGGGCCGGATTACTGCATGATGAAACGGCGGGTATTTCTCATGGCACTTACATCGCATTGATGATGAACGGCAAAGCAATGCCGAAAGATCCa            >  1:661763/4‑150 (MQ=255)
                                                                                                                                         caaccGGCAGTCCTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGTATGATGAAACTGCGGTAATTTCTCATGGCACTTACATCGCATTGATGATGAACTGCAAAGCACTGCCGAAAGATCCa            >  1:331524/4‑150 (MQ=255)
                                                                                                                                         caaccGGCAGTCCTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATtgatg                                          <  2:760835/117‑1 (MQ=255)
                                                                                                                                         caaccGGCAGTCCTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATtgatg                                          >  1:760835/4‑120 (MQ=255)
                                                                                                                                         caaccGGCAGTCCTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCa            >  2:1408611/4‑150 (MQ=255)
                                                                                                                                            ccGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACGGGTAGAAGCTCTGGGGGCCGGTTTACTGGATGATGAAACGGCGGTATTTTCTGTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTa         <  2:173695/150‑1 (MQ=255)
                                                                                                                                              ggCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCATGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGc       <  2:356645/150‑1 (MQ=255)
                                                                                                                                                   gACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACt  <  1:1436620/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATTATAAGAGCTGGGCATGGAGCGAAGATGCTGCGGTAATGGATAAGTTTAATATCCCCCGTCATATGCTGTTTGATGTGCAAATGCCTGGCACCGTCCTCGGACATATCACACCACAAGCCGCACTGGCGACACATTTCCCGGCAGGACTGCCGGTTGTTTGTACCACCAGTGATAAACCGGTAGAAGCTCTGGGGGCCGGATTACTGGATGATGAAACGGCGGTAATTTCTCTTGGCACTTACATCGCATTGATGATGAACGGCAAAGCACTGCCGAAAGATCCAGTAGCGTACT  >  NC_000913/2902347‑2902643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: