Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 289,256 G→C 8.1% intergenic (‑94/+45) yagI ← / ← argF CP4‑6 prophage; putative DNA‑binding transcriptional regulator/ornithine carbamoyltransferase 2, chain F; CP4‑6 prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913289,2560GC 8.1% 222.6 / 10.4 74intergenic (‑94/+45)yagI/argFCP4‑6 prophage; putative DNA‑binding transcriptional regulator/ornithine carbamoyltransferase 2, chain F; CP4‑6 prophage
Reads supporting (aligned to +/- strand):  ref base G (50/18);  new base C (3/3);  total (53/21)
Fisher's exact test for biased strand distribution p-value = 3.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.17e-01

TCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCAAACACCTCGTCCGT  >  NC_000913/289107‑289402
                                                                                                                                                       |                                                                                                                                                    
tCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCtg                                                                                                                                                      <  1:585582/150‑1 (MQ=255)
 cGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCtgt                                                                                                                                                     >  1:1668781/1‑150 (MQ=255)
  gTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCtgtg                                                                                                                                                    <  1:99862/150‑1 (MQ=255)
   ttGATCAGGGCGAGGATCTGCAACGGACTTTCAACAGACTTAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTgg                                                                                                                                                   <  2:1565762/150‑1 (MQ=255)
    tGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTggg                                                                                                                                                  <  2:1440877/150‑1 (MQ=255)
     gAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAAAAATCGTCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACCTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGga                                                                                                                                                 <  1:159312/150‑1 (MQ=255)
         atgTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCACAGGGAGAgg                                                                                                                                            <  2:606394/148‑1 (MQ=255)
          ggTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAggg                                                                                                                                           <  2:1012618/150‑1 (MQ=255)
          ggTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCACAGGGAGAggg                                                                                                                                           <  2:611010/150‑1 (MQ=255)
           gTCGAGGATCTGCAACGCCCGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCCCGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGc                                                                                                                                          >  1:953754/1‑150 (MQ=255)
           gTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCACAGGGAGAggga                                                                                                                                          <  1:1623266/150‑2 (MQ=255)
               aGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCggg                                                                                                                                      <  1:1024330/150‑1 (MQ=255)
                ggATCTGCAACGCACGTTCAACAGACTGAATGATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCgggg                                                                                                                                     >  1:1335507/1‑150 (MQ=255)
                   tCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGa                                                                                                                                  <  2:1597613/150‑1 (MQ=255)
                      gCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCATGTGGGAGAGGGCCGGGGTGAggg                                                                                                                              >  1:844449/1‑150 (MQ=255)
                       cAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGc                                                                                                                             >  1:997377/1‑150 (MQ=255)
                         aCGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAc                                                                                                                           >  2:1302081/1‑150 (MQ=255)
                         aCGCACGTTCAACAGACTGAATAAGCGGCATAATGCTGGCATGTCCACGCGGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAc                                                                                                                           <  1:388/150‑1 (MQ=255)
                          cGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAcc                                                                                                                          >  1:510315/1‑150 (MQ=255)
                           gCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCa                                                                                                                         >  1:1011292/1‑150 (MQ=255)
                                  cAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTTAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCa                                                                                                                  >  2:555499/1‑150 (MQ=255)
                                  cAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAAGGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCa                                                                                                                  >  1:859880/1‑150 (MQ=255)
                                   aaCAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCCCAACGCGCAg                                                                                                                 >  1:704602/1‑150 (MQ=255)
                                         cTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGAcc                                                                                                           >  1:550134/1‑150 (MQ=255)
                                         cTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGAcc                                                                                                           >  2:454387/1‑150 (MQ=255)
                                           gAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGCCCCa                                                                                                         >  2:836624/1‑150 (MQ=255)
                                                atCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCAc                                                                                                    >  1:1024563/1‑150 (MQ=255)
                                                atCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCCGACCCCATCAc                                                                                                    >  2:590569/1‑150 (MQ=255)
                                                    ccATAATGCTGGCAGGCCCCCCGCGGTATAACGGCTAACCCGAACCTTTTGCGGTATTAAAAATCAATGACCGGCCGCCGGGGTCCCCCCGCCCCCTGTGCGTGTGGGCCGGGGTGCGGGCACCAACGCGCAGCGGACCCAATCACTccc                                                                                                <  2:346821/149‑1 (MQ=255)
                                                        aaTGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCacg                                                                                            >  2:569068/1‑148 (MQ=255)
                                                          tGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCg                                                                                          >  1:385585/1‑150 (MQ=255)
                                                           gCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGt                                                                                         >  2:912564/1‑150 (MQ=255)
                                                             tGGAATGTCCACGCTGCAATTAAGGCGAAACCTTCCTTTTTTGGGGATGAAAAATCATTGAATTGGCGCGGGTGTTCTCCCTCTCCGGGGGGGTGTGGGCGGGGGGGTGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTg                                                                                       <  1:5567/150‑1 (MQ=255)
                                                             gggCATGCCCACGCTGTAATTAAGGCTAAAACGTACCTTTTTCGGTATTAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGGGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTg                                                                                       <  2:1012597/149‑1 (MQ=38)
                                                             gggCATGCCCACGCGGGAATTAAGGCGAAAAGGTACCTTTTTCGGGATGAAAAATCAATGAAATGGCGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGGGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTg                                                                                       <  2:1202605/149‑1 (MQ=39)
                                                              ggCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGc                                                                                      >  1:411003/1‑150 (MQ=255)
                                                                cATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCCAGCGTTGCca                                                                                    >  2:465113/1‑150 (MQ=255)
                                                                  tGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCACGCGTTGCcatc                                                                                  >  2:1683463/1‑150 (MQ=255)
                                                                   gTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCcatca                                                                                 >  1:398057/1‑150 (MQ=255)
                                                                   gTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCcatca                                                                                 >  2:914796/1‑150 (MQ=255)
                                                                         gCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCg                                                                           >  1:1159779/1‑150 (MQ=255)
                                                                         gCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCAGGGGTGAGCGCACCAACTCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATAACCg                                                                           >  1:156381/1‑150 (MQ=255)
                                                                            ggAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTAGCCATCATCACCGCCt                                                                        >  1:606916/1‑150 (MQ=255)
                                                                             gAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCtt                                                                       >  2:580513/1‑150 (MQ=255)
                                                                                ttAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCATGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGCCCCAATCCCTCCCCACCCGTGGCCATCATCACCGCCTaaaa                                                                    >  2:872435/1‑149 (MQ=38)
                                                                                  aaGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCg                                                                  >  1:462236/1‑150 (MQ=255)
                                                                                    ggCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTa                                                                >  1:985420/1‑150 (MQ=255)
                                                                                       taaaaCGTCTCTTTTTTGGTATATAAAAATCATTGATAGTGGGCGGGGGTTCCCCCGCTCGCTGGGTGCTGGGGCGGGGGTGGGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATg                                                              <  2:704602/149‑1 (MQ=255)
                                                                                        aaaaCGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGAGTTGCCAACATCACCGCCTTAAACGTATgaa                                                            >  2:351164/1‑148 (MQ=255)
                                                                                            cGTACCTTTTTCGGGATGAAAAATCAATGAACTGGAGCCGGTGTTCTCCCTCTCCATGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCg                                                        <  2:844449/150‑1 (MQ=255)
                                                                                            cGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGCCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCTTCCGCGCCTTAAACGTATGCATCCg                                                        >  2:1247784/1‑150 (MQ=39)
                                                                                                 cTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGAGGGAGAGGGCCGGGGTGAGGGCTCCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGTtttt                                                   >  1:1586122/1‑150 (MQ=255)
                                                                                                    tttCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCg                                                >  1:1210348/1‑150 (MQ=255)
                                                                                                         ggATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGTCCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTgg                                           >  2:1364219/1‑150 (MQ=255)
                                                                                                         ggATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTgg                                           >  1:1225111/1‑150 (MQ=255)
                                                                                                          gATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGATGGCCGGGGTGAGGGCACCAACGCGCAGCCGCCCCAATCACACCCCAAGCGTTGCCATCCTCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTggg                                          >  2:1637175/1‑149 (MQ=39)
                                                                                                               aaaaaTCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGaa                                      >  1:1295578/1‑150 (MQ=255)
                                                                                                               aaaaaTCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCATAATCGTATGCATCCGGTATTCCGCCTGGTCGaa                                      >  2:392004/1‑150 (MQ=255)
                                                                                                                aaaaTCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAac                                     >  1:1717436/1‑150 (MQ=255)
                                                                                                                 aaaTCAATTAAATGGCGCCGGTGTGCTCCCTCTCCCTGGGGGTGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAaca                                    <  1:991562/150‑1 (MQ=255)
                                                                                                                 aaaTCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAaca                                    >  2:866006/1‑150 (MQ=255)
                                                                                                                 aaaTCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAaca                                    >  2:1025604/1‑150 (MQ=255)
                                                                                                                        tGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCACGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCt                             >  2:1175846/1‑150 (MQ=255)
                                                                                                                        tGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCt                             >  2:968632/1‑150 (MQ=255)
                                                                                                                                aGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCCCCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGATCACGATGCTCGccgccg                     >  1:303638/1‑150 (MQ=255)
                                                                                                                                   gggTGTGCCCCCTCCCCTGGGGGGTGTGGGCGGGGGTGGGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACt                  <  2:550134/149‑1 (MQ=255)
                                                                                                                                   cGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACt                  >  1:1584104/1‑150 (MQ=255)
                                                                                                                                    gggtttCTCCCCCTACCCGGTGGGGGTGCGGGCGGGGTGGGGCCCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGa                    <  1:1453233/146‑1 (MQ=255)
                                                                                                                                      ccccggccctctcccaCAGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACGCaa               >  2:240550/7‑150 (MQ=255)
                                                                                                                                        ccggccctctcccaCAGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGGATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCaacc             >  2:918446/5‑148 (MQ=255)
                                                                                                                                          ctccctctccctGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCAAacac           >  1:1047745/1‑150 (MQ=255)
                                                                                                                                           gccctctcccaCAGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCAAACAcc          >  2:41653/2‑150 (MQ=255)
                                                                                                                                            ccctctccctGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCAAACAccg         >  2:335997/1‑149 (MQ=255)
                                                                                                                                                ctccttGGGGGTGGGGGCGGGGGGGGGGCCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCAAACACCTcgtc     <  2:411003/150‑1 (MQ=255)
                                                                                                                                                   ccctGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGCTGCTCGCCGCCGACTCAAACACCTcgtccg   >  2:904459/1‑150 (MQ=255)
                                                                                                                                                   ccctGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCAAACACCTcgtccg   <  2:1024563/150‑1 (MQ=255)
                                                                                                                                                    cctGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCAAACACCTcgtccgt  >  2:221807/1‑150 (MQ=255)
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TCGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCAAACACCTCGTCCGT  >  NC_000913/289107‑289402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: