Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,907,998 A→T 10.1% intergenic (‑57/+31) eno ← / ← pyrG enolase/CTP synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,907,9980AT10.1% 387.6 / 28.7 156intergenic (‑57/+31)eno/pyrGenolase/CTP synthetase
Reads supporting (aligned to +/- strand):  ref base A (72/68);  new base T (11/5);  total (83/73)
Fisher's exact test for biased strand distribution p-value = 2.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.42e-01

Only 100 of 156 total aligned reads displayed.

AAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACC  >  NC_000913/2907850‑2908144
                                                                                                                                                    |                                                                                                                                                  
   ccaccCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGt                                                                                                                                                >  2:707695/1‑150 (MQ=255)
   ccaccCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGt                                                                                                                                                <  1:569709/150‑1 (MQ=255)
    caccCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTa                                                                                                                                               >  2:816922/1‑150 (MQ=255)
     accCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTAc                                                                                                                                              <  2:205787/150‑1 (MQ=255)
      cccTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCTGGGTAcg                                                                                                                                             <  1:883688/150‑1 (MQ=255)
        cTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTAcgcg                                                                                                                                           >  2:48504/1‑150 (MQ=255)
         tCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGt                                                                                                                                          <  1:1123356/150‑1 (MQ=255)
          ccAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGtt                                                                                                                                         <  1:816922/150‑1 (MQ=255)
          ccAGATGTACATCGTCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAACCCCCAGACAAACAACGCGCACCCAGGGTACGCGtt                                                                                                                                         <  1:239179/150‑1 (MQ=255)
            aGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGc                                                                                                                                       >  2:126703/1‑150 (MQ=255)
             gATGTACTTCGGCTTCAACAGGCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCTGGGTACGCGTTgtt                                                                                                                                      <  2:1170849/150‑3 (MQ=255)
                gTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCTGGGTACGCGTTgtttgt                                                                                                                                   <  2:1635663/150‑6 (MQ=255)
                 tACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGctc                                                                                                                                  <  1:1010823/150‑1 (MQ=255)
                     tCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAAc                                                                                                                              >  1:1233612/1‑150 (MQ=255)
                        gCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACttt                                                                                                                           >  2:333638/1‑150 (MQ=255)
                        gCTTCAACAGTCGGGTTACAACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGAGTACCCAGGGTACGCGTTGCCGCTCTAACttt                                                                                                                           >  1:5326/1‑150 (MQ=255)
                         cTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACtttt                                                                                                                          >  1:608338/1‑150 (MQ=255)
                          ttacaCAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTAACGATTTTGTACATTAGGTTTTCCTCAAGTCAATATTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACttttt                                                                                                                         <  1:1547520/146‑1 (MQ=255)
                             accAGTCCGGTGACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTCCCCAGGGTACGCGTTGCCGCTCTAACTTTTttac                                                                                                                      <  2:236818/148‑1 (MQ=255)
                                aGTCGGGTTACCGCGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTttactta                                                                                                                   >  1:1224754/1‑150 (MQ=255)
                                    gggTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTc                                                                                                               >  1:601410/1‑150 (MQ=255)
                                     ggTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCg                                                                                                              <  2:395799/150‑1 (MQ=255)
                                     ggTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCg                                                                                                              >  2:1151127/1‑150 (MQ=255)
                                       ttACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGcc                                                                                                            <  2:901264/150‑1 (MQ=255)
                                         agcACGGTAGTCGATGAGTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAATCTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTg                                                                                                          <  2:963370/148‑1 (MQ=255)
                                            aCGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACg                                                                                                       >  1:449419/1‑150 (MQ=255)
                                              gggAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGtt                                                                                                     <  2:1385865/150‑1 (MQ=255)
                                                  gTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTg                                                                                                 <  2:426607/150‑1 (MQ=255)
                                                  gTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTg                                                                                                 >  1:524128/1‑150 (MQ=255)
                                                   tCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACACGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTgg                                                                                                <  2:1253268/150‑1 (MQ=255)
                                                    cGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGa                                                                                               >  2:158772/1‑150 (MQ=255)
                                                    cGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCATGACGTTTCTGGa                                                                                               >  2:943049/1‑150 (MQ=255)
                                                    cGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCAGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGa                                                                                               >  2:450277/1‑150 (MQ=255)
                                                     gatgatTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTg                                                                                                 >  2:1128094/1‑147 (MQ=255)
                                                     gatgatTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTg                                                                                                 <  1:1128094/147‑1 (MQ=255)
                                                        gatTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTc                                                                                           >  2:532492/1‑150 (MQ=255)
                                                         atTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCg                                                                                          >  1:917723/1‑150 (MQ=255)
                                                          tttCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGc                                                                                         <  1:1717623/150‑1 (MQ=255)
                                                           ttCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGCCAAACAACGCGTACCCAGCGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGATTCTGGAACTCGCt                                                                                        >  2:724208/1‑150 (MQ=255)
                                                               cGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTggcg                                                                                    <  1:1523737/150‑1 (MQ=255)
                                                                gACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTggcgg                                                                                   <  2:1528408/150‑1 (MQ=255)
                                                                  ccGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCt                                                                                 >  1:571186/1‑150 (MQ=255)
                                                                        atTTTTACGATTTAGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAACCAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTcaca                                                                           <  1:261471/150‑1 (MQ=255)
                                                                           tttACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGATCGCGCTGGCGGCTTTCACAAAg                                                                        >  1:866595/1‑150 (MQ=255)
                                                                           tttACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAg                                                                        <  1:1618969/150‑1 (MQ=255)
                                                                            ttACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCGCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGc                                                                       <  2:115418/150‑1 (MQ=255)
                                                                            ttACGATTTTGGACATTAGGATTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGc                                                                       <  2:1399803/150‑1 (MQ=255)
                                                                              aCGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCt                                                                     >  2:704094/1‑150 (MQ=255)
                                                                               cGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTACCTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTg                                                                    >  2:679489/1‑150 (MQ=255)
                                                                                gATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGc                                                                   <  1:1397088/150‑1 (MQ=255)
                                                                                gATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGc                                                                   <  1:323157/150‑1 (MQ=255)
                                                                                gATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGc                                                                   >  2:1569351/1‑150 (MQ=255)
                                                                                 aTTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTTACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCa                                                                  <  1:1461610/150‑1 (MQ=255)
                                                                                    ttGGACATTAGGTTATCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTATTTACTAACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAAc                                                               <  2:1618075/150‑1 (MQ=255)
                                                                                     tGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCTGATTTCACAAAGCCTGCAAACa                                                              >  1:324674/1‑150 (MQ=255)
                                                                                      ggACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAg                                                             >  1:1202148/1‑150 (MQ=255)
                                                                                       gACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGc                                                            <  2:903415/150‑1 (MQ=255)
                                                                                        aCATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCg                                                           <  2:673617/150‑1 (MQ=255)
                                                                                          aTTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCggg                                                         <  2:571186/150‑1 (MQ=255)
                                                                                          aTTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCggg                                                         <  2:601410/150‑1 (MQ=255)
                                                                                           ttAGGTGTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACACCGCGTACCCAGGGAACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGt                                                        <  1:1299047/150‑1 (MQ=255)
                                                                                              ggTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGAc                                                     >  2:1355014/1‑150 (MQ=255)
                                                                                              ggTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGAc                                                     >  1:301316/1‑150 (MQ=255)
                                                                                              ggTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGAc                                                     >  1:920108/1‑150 (MQ=255)
                                                                                                ttttCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCa                                                   >  2:1314132/1‑150 (MQ=255)
                                                                                                    ccTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCAc                                               >  1:1398974/1‑150 (MQ=255)
                                                                                                     cTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACg                                              >  2:1656778/1‑150 (MQ=255)
                                                                                                     cTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACg                                              >  1:1172693/1‑150 (MQ=255)
                                                                                                      tCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGt                                             >  2:928770/1‑150 (MQ=255)
                                                                                                        aaGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTgg                                           <  2:1202148/150‑1 (MQ=255)
                                                                                                        aaGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTgg                                           <  2:1371734/150‑1 (MQ=255)
                                                                                                        aaGTCACAAGTTAAACTGAAACTCCAGACAAACACCGCGTACCCTGGGAACGCGTTGCCGCTCTAACTTTTTTACTTACAACGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTgg                                           <  1:724208/150‑1 (MQ=255)
                                                                                                         aGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGa                                          >  2:338516/1‑150 (MQ=255)
                                                                                                          gTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAg                                         >  1:693957/1‑150 (MQ=255)
                                                                                                           tCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGt                                        <  1:992352/150‑1 (MQ=255)
                                                                                                             aCTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAg                                      >  2:8171/1‑150 (MQ=255)
                                                                                                                aGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAg                                   >  1:1235330/1‑150 (MQ=255)
                                                                                                                   tAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTaa                                >  1:355227/1‑150 (MQ=255)
                                                                                                                         aaaaCACCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCg                          >  1:835611/2‑150 (MQ=255)
                                                                                                                            aCTCCATACAAACAACGAGTACCAAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGAt                       <  2:1221022/150‑1 (MQ=255)
                                                                                                                               ccAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGa                    >  1:346612/1‑150 (MQ=255)
                                                                                                                                   acaaacaaCGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTg                <  1:1378258/150‑1 (MQ=255)
                                                                                                                                   acaaacaaCGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTg                <  2:1541479/150‑1 (MQ=255)
                                                                                                                                   acaaacaaCGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGGTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTg                <  1:126703/150‑1 (MQ=255)
                                                                                                                                    gcggcaacGCGTACCCTGGGTACGCGTTGTCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTgg               >  2:1132320/5‑150 (MQ=255)
                                                                                                                                      ggcaacGCGTACCCTGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCa             >  1:419664/3‑150 (MQ=255)
                                                                                                                                      aacaacGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCa             >  2:544335/1‑150 (MQ=255)
                                                                                                                                      aacaacGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCa             >  1:1210211/1‑150 (MQ=255)
                                                                                                                                      aacaacGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCa             >  1:1168560/1‑150 (MQ=255)
                                                                                                                                        caacGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGCCCATCACGTGGAGTAGAAGTCATCTCAGGATGGAACTGGCAAg           >  1:1035406/1‑150 (MQ=255)
                                                                                                                                         aacGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGc          >  2:626010/1‑150 (MQ=255)
                                                                                                                                         aacGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGc          <  2:301316/150‑1 (MQ=255)
                                                                                                                                         aacGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGc          >  1:18364/1‑150 (MQ=255)
                                                                                                                                         aacGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGc          >  1:420185/1‑150 (MQ=255)
                                                                                                                                           cgcgTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCa        <  1:1632269/150‑1 (MQ=255)
                                                                                                                                           cgcgTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCa        <  1:704094/150‑1 (MQ=255)
                                                                                                                                             cgTACCCTGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACg      >  2:1226655/1‑150 (MQ=255)
                                                                                                                                              gTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGa     <  1:135840/150‑1 (MQ=255)
                                                                                                                                                 cccAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAAcc  >  2:30307/1‑150 (MQ=255)
                                                                                                                                                 cccAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAAcc  <  2:189914/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AAACCACCCTCCAGATGTACTTCGGCTTCAACAGTCGGGTTACCACGGGAGTCGATGATTTCACGACCGATGATTTTTACGATTTTGGACATTAGGTTTTCCTCAAGTCACTAGTTAAACTGAAACTCCAGACAAACAACGCGTACCCAGGGTACGCGTTGCCGCTCTAACTTTTTTACTTACTTCGCCTGACGTTTCTGGAACTCGCTGGCGGCTTTCACAAAGCCTGCAAACAGCGGGTGACCATCACGTGGAGTAGAAGTAAACTCCGGATGGAACTGGCAAGCCACGAACC  >  NC_000913/2907850‑2908144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑