Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,911,008 A→T 7.4% H28Q (CAT→CAA) ‡ mazF ← mRNA interferase toxin, antitoxin is MazE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,911,0080AT 7.4% 411.4 / 16.4 144H28Q (CAT→CAA) ‡mazFmRNA interferase toxin, antitoxin is MazE
Reads supporting (aligned to +/- strand):  ref base A (63/69);  new base T (4/7);  total (67/77)
Fisher's exact test for biased strand distribution p-value = 5.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.85e-01

Only 100 of 144 total aligned reads displayed.

CCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTG  >  NC_000913/2910860‑2911154
                                                                                                                                                    |                                                                                                                                                  
ccTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGAt                                                                                                                                                   >  2:938411/1‑150 (MQ=255)
 cTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATg                                                                                                                                                  <  1:681759/150‑1 (MQ=255)
 cTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATg                                                                                                                                                  <  1:1519253/150‑1 (MQ=255)
  tGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGt                                                                                                                                                 >  1:1249876/1‑150 (MQ=255)
   gATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTc                                                                                                                                                >  2:393959/1‑150 (MQ=255)
    aTCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTcc                                                                                                                                               <  1:521845/150‑1 (MQ=255)
    aTCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTcc                                                                                                                                               <  1:957683/150‑1 (MQ=255)
      cAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAg                                                                                                                                             >  1:917545/1‑150 (MQ=255)
       aGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGc                                                                                                                                            <  1:375405/150‑1 (MQ=255)
        gCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCt                                                                                                                                           >  2:108802/1‑150 (MQ=255)
        gCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCt                                                                                                                                           <  1:963714/150‑1 (MQ=255)
        gCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCt                                                                                                                                           <  1:714732/150‑1 (MQ=255)
        ccTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATCTCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCt                                                                                                                                           <  1:1487786/149‑1 (MQ=255)
          tAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACATCGTCCAGCtgt                                                                                                                                         <  2:681808/150‑3 (MQ=255)
          tAACGCTACGCCAGCACGTTCCTGACCGGATAAACCAACTTAGAACGGATAACCTTTTCATTGCGTTGTACCTGGACCACACAGACACATACCTGTTGTGTTGTTCTACATGAAAGGCCTCCGGACAACAGCTGGACGATGTCAAGCTTg                                                                                                                                         >  1:271610/1‑150 (MQ=255)
           aaCGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGATGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACATCGTCCAGCtgtt                                                                                                                                        <  1:964096/150‑4 (MQ=255)
            aCGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACATCGTCCAGCtgttg                                                                                                                                       <  1:1179333/150‑5 (MQ=255)
              gCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCg                                                                                                                                     >  2:1468578/1‑150 (MQ=255)
              gCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACATCGTCCAGCtgttgtc                                                                                                                                     <  1:1112830/150‑7 (MQ=255)
              ccTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAACGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCg                                                                                                                                     <  1:1451533/149‑1 (MQ=255)
                    ccATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCt                                                                                                                               >  2:1694091/1‑150 (MQ=255)
                    ccATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCt                                                                                                                               >  2:63416/1‑150 (MQ=255)
                     cATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCtt                                                                                                                              >  2:1418999/1‑150 (MQ=255)
                        cACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGATTGCTCGCTACCTTTTg                                                                                                                           >  2:1446066/1‑150 (MQ=255)
                         aCGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGt                                                                                                                          <  1:1240365/150‑1 (MQ=255)
                           gTTCCTGCCCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCg                                                                                                                        >  1:447380/1‑150 (MQ=255)
                              ccTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGt                                                                                                                     >  2:956122/1‑150 (MQ=255)
                              ccTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGt                                                                                                                     <  1:1418999/150‑1 (MQ=255)
                               cTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTc                                                                                                                    >  2:745223/1‑150 (MQ=255)
                                tGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCa                                                                                                                   >  1:1274715/1‑150 (MQ=255)
                                   ccGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCaaaa                                                                                                                >  2:183392/1‑150 (MQ=255)
                                    cGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAAt                                                                                                               >  1:605612/1‑150 (MQ=255)
                                     ggATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATc                                                                                                              <  2:1182273/150‑1 (MQ=255)
                                        tAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAAc                                                                                                           <  2:1126266/150‑1 (MQ=255)
                                          naacaacTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGCACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGGCAAAGTCAAccc                                                                                                         >  2:418888/2‑150 (MQ=255)
                                          aaaCAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAAccc                                                                                                         >  2:683806/1‑150 (MQ=255)
                                          aaaCAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAAccc                                                                                                         >  1:1213706/1‑150 (MQ=255)
                                                cTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCa                                                                                                   >  1:405985/1‑150 (MQ=255)
                                                    gAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATc                                                                                               >  2:1463388/1‑150 (MQ=255)
                                                      aCGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGc                                                                                             <  1:63416/150‑1 (MQ=255)
                                                         gctctCCTGTTGATTGCGTGGTCAAAGGCACCCAACGACACATACCTGGTGTGTGGTTGTACAGGAAAGGCCTCAGGCCCACAGCTGGACGCTGTCCAGCTTGCTCGCTACCGTTTGTCGGGTCAAAATCAACCCACATCCGATCGCCCa                                                                                          <  1:229661/146‑1 (MQ=255)
                                                          atatCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCat                                                                                         <  2:1267516/150‑1 (MQ=255)
                                                             tCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATc                                                                                      >  2:207837/1‑150 (MQ=255)
                                                             tCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATc                                                                                      <  2:1333407/150‑1 (MQ=255)
                                                              ccTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCg                                                                                     >  2:947629/1‑150 (MQ=255)
                                                                ttttGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCggg                                                                                   <  2:425491/150‑1 (MQ=255)
                                                                ttttGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCCGGTCAAAATCAACCCAAATCAGATCGCCCATATCggg                                                                                   >  1:62044/1‑150 (MQ=255)
                                                                 tttGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGt                                                                                  <  1:393959/150‑1 (MQ=255)
                                                                    gATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACg                                                                               >  1:865300/1‑150 (MQ=255)
                                                                     aTTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGt                                                                              >  1:1018131/1‑150 (MQ=255)
                                                                      ttGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTa                                                                             >  1:384374/1‑150 (MQ=255)
                                                                        gCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATc                                                                           <  1:1577242/150‑1 (MQ=255)
                                                                        gCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCAAAATCAGATCGCCCATATCGGGTACGTATc                                                                           >  1:728315/1‑150 (MQ=255)
                                                                         cGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGTAAGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCg                                                                          <  2:1273655/150‑1 (MQ=255)
                                                                         cGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCAAATCGGGTACGTATCg                                                                          >  1:1643116/1‑150 (MQ=255)
                                                                            tGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCt                                                                       <  1:1028660/150‑1 (MQ=255)
                                                                            tGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCt                                                                       >  2:558299/1‑150 (MQ=255)
                                                                              tACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGTGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCtta                                                                     <  2:1209362/150‑1 (MQ=255)
                                                                               aCAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCttac                                                                    <  1:1104849/150‑1 (MQ=255)
                                                                               aCAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCttac                                                                    <  1:938411/150‑1 (MQ=255)
                                                                                cAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCttacc                                                                   >  2:1019426/1‑150 (MQ=255)
                                                                                cAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCttacc                                                                   >  1:174356/1‑150 (MQ=255)
                                                                                     aaCACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCttaccatacc                                                              <  1:683806/150‑4 (MQ=255)
                                                                                      acacacAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCttaccattacc                                                             <  2:1249876/150‑1 (MQ=255)
                                                                                       cacacaGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCttaccattacca                                                            >  2:790970/1‑150 (MQ=255)
                                                                                       cacacaGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCttaccattacca                                                            >  2:163405/1‑150 (MQ=255)
                                                                                        acacaGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTACAGCTTGATCGCAACCTTTTGTCGGGTCAAAATAAACCCAAATCAGATCGCCCATAACGTGTACGTATCGGCttaccattaccaa                                                           >  2:1588801/1‑149 (MQ=255)
                                                                                         cacaGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGa                                                          >  2:466425/1‑150 (MQ=255)
                                                                                         cacaGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGa                                                          >  1:568382/1‑150 (MQ=255)
                                                                                          acaGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGAc                                                         <  1:1329320/150‑1 (MQ=255)
                                                                                           caGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACt                                                        >  2:1312691/1‑150 (MQ=255)
                                                                                           caGACACATACCTGTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACtt                                                       >  1:442105/1‑150 (MQ=255)
                                                                                            aGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACtt                                                       <  2:917545/150‑1 (MQ=255)
                                                                                               ctcaTACCTGAGTTGTTTTTGTACATGAAAGGACGCAGGACTACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGAATCGGCTTACCATAACCAGACTTCCt                                                    <  2:706705/148‑1 (MQ=255)
                                                                                                acaTACCTGTTTTGTTGGTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCtt                                                   <  2:271610/150‑1 (MQ=255)
                                                                                                 caTACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTa                                                  <  1:956122/150‑1 (MQ=255)
                                                                                                 caTACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTa                                                  >  1:661992/1‑150 (MQ=255)
                                                                                                   tACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATc                                                <  1:1681566/150‑1 (MQ=255)
                                                                                                   tACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATc                                                >  2:648497/1‑150 (MQ=255)
                                                                                                    aCCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGAc                                                         <  2:65969/140‑1 (MQ=255)
                                                                                                       tGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTc                                            <  2:1095592/150‑1 (MQ=255)
                                                                                                       tGTTTTGTTGTTGAACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTc                                            <  2:278041/150‑1 (MQ=255)
                                                                                                            tgttgttgtACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGctc                                       >  2:493420/1‑150 (MQ=255)
                                                                                                                 ttgtAGATGAATGGACTCAGGACATCAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTcccc                                  <  2:1234068/150‑1 (MQ=255)
                                                                                                                 ttgtACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTcccc                                  <  2:174356/150‑1 (MQ=255)
                                                                                                                  tgtACATGAAAGGTATCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGGCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGGATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCa                                 <  1:1135477/150‑1 (MQ=255)
                                                                                                                      aaTGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCg                             <  2:1505407/149‑1 (MQ=255)
                                                                                                                         gAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGc                                         <  2:38446/135‑1 (MQ=255)
                                                                                                                         gAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGata                          >  1:651822/1‑150 (MQ=255)
                                                                                                                         gAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGata                          <  1:1019426/150‑1 (MQ=255)
                                                                                                                           aaGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATAtt                        >  2:1296790/1‑150 (MQ=255)
                                                                                                                                 tCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTg                  <  2:1018131/150‑1 (MQ=255)
                                                                                                                                 tCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTg                  <  1:543292/150‑1 (MQ=255)
                                                                                                                                    gcgagcaaGCTGGACATCGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCACCCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAg               >  1:1018153/8‑150 (MQ=255)
                                                                                                                                       caacaGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGtt            <  1:1717897/150‑1 (MQ=255)
                                                                                                                                         acaGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGtttt          >  2:569899/1‑150 (MQ=255)
                                                                                                                                          aaGCTGGACATCGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCGGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTc         >  1:652684/2‑150 (MQ=255)
                                                                                                                                          aaGCTGGACATCGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTc         >  1:346579/2‑150 (MQ=255)
                                                                                                                                             cTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCgg      <  1:558299/150‑1 (MQ=255)
                                                                                                                                             cTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGGACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCgg      <  1:108802/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CCTGATCAGCTAACGCTACGCCATCACGTTCCTGACCGGATAAAACAACTTCGAACGGATATCCTTTTGATTGCGTTGTACAAGGAACACACAGACACATACCTGTTTTGTTGTTGTACATGAAAGGACTCAGGACAACAGCTGGACGATGTCCAGCTTGCTCGCTACCTTTTGTCGGGTCAAAATCAACCCAAATCAGATCGCCCATATCGGGTACGTATCGGCTTACCATTACCAGACTTCCTTATCTTTCGGCTCTCCCCAGTCGATATTCTCGTGGAGGTTTTCCGGCGTG  >  NC_000913/2910860‑2911154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: