Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 289,257 T→A 8.9% intergenic (‑95/+44) yagI ← / ← argF CP4‑6 prophage; putative DNA‑binding transcriptional regulator/ornithine carbamoyltransferase 2, chain F; CP4‑6 prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913289,2570TA 8.9% 173.4 / 5.4 74intergenic (‑95/+44)yagI/argFCP4‑6 prophage; putative DNA‑binding transcriptional regulator/ornithine carbamoyltransferase 2, chain F; CP4‑6 prophage
Reads supporting (aligned to +/- strand):  ref base T (49/13);  new base A (4/3);  total (53/21)
Fisher's exact test for biased strand distribution p-value = 3.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCAAACACCTCGTCCGTCACC  >  NC_000913/289108‑289406
                                                                                                                                                       |                                                                                                                                                       
cGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCtgt                                                                                                                                                         >  1:1668781/1‑150 (MQ=255)
 gTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCtgtg                                                                                                                                                        <  1:99862/150‑1 (MQ=255)
  ttGATCAGGGCGAGGATCTGCAACGGACTTTCAACAGACTTAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTgg                                                                                                                                                       <  2:1565762/150‑1 (MQ=255)
   tGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTggg                                                                                                                                                      <  2:1440877/150‑1 (MQ=255)
    gAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAAAAATCGTCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACCTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGga                                                                                                                                                     <  1:159312/150‑1 (MQ=255)
        atgTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCACAGGGAGAgg                                                                                                                                                <  2:606394/148‑1 (MQ=255)
         ggTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAggg                                                                                                                                               <  2:1012618/150‑1 (MQ=255)
         ggTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCACAGGGAGAggg                                                                                                                                               <  2:611010/150‑1 (MQ=255)
          gTCGAGGATCTGCAACGCCCGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCCCGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGc                                                                                                                                              >  1:953754/1‑150 (MQ=255)
          gTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCACAGGGAGAggga                                                                                                                                              <  1:1623266/150‑2 (MQ=255)
              aGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCggg                                                                                                                                          <  1:1024330/150‑1 (MQ=255)
               ggATCTGCAACGCACGTTCAACAGACTGAATGATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCgggg                                                                                                                                         >  1:1335507/1‑150 (MQ=255)
                  tCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGa                                                                                                                                      <  2:1597613/150‑1 (MQ=255)
                     gCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCATGTGGGAGAGGGCCGGGGTGAggg                                                                                                                                  >  1:844449/1‑150 (MQ=255)
                      cAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGc                                                                                                                                 >  1:997377/1‑150 (MQ=255)
                        aCGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAc                                                                                                                               >  2:1302081/1‑150 (MQ=255)
                        aCGCACGTTCAACAGACTGAATAAGCGGCATAATGCTGGCATGTCCACGCGGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAc                                                                                                                               <  1:388/150‑1 (MQ=255)
                         cGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAcc                                                                                                                              >  1:510315/1‑150 (MQ=255)
                          gCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCa                                                                                                                             >  1:1011292/1‑150 (MQ=255)
                                 cAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTTAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCa                                                                                                                      >  2:555499/1‑150 (MQ=255)
                                 cAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAAGGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCa                                                                                                                      >  1:859880/1‑150 (MQ=255)
                                  aaCAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCCCAACGCGCAg                                                                                                                     >  1:704602/1‑150 (MQ=255)
                                        cTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGAcc                                                                                                               >  2:454387/1‑150 (MQ=255)
                                        cTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGAcc                                                                                                               >  1:550134/1‑150 (MQ=255)
                                          gAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGCCCCa                                                                                                             >  2:836624/1‑150 (MQ=255)
                                               atCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCAc                                                                                                        >  1:1024563/1‑150 (MQ=255)
                                               atCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCCGACCCCATCAc                                                                                                        >  2:590569/1‑150 (MQ=255)
                                                   ccATAATGCTGGCAGGCCCCCCGCGGTATAACGGCTAACCCGAACCTTTTGCGGTATTAAAAATCAATGACCGGCCGCCGGGGTCCCCCCGCCCCCTGTGCGTGTGGGCCGGGGTGCGGGCACCAACGCGCAGCGGACCCAATCACTccc                                                                                                    <  2:346821/149‑1 (MQ=255)
                                                       aaTGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCacg                                                                                                >  2:569068/1‑148 (MQ=255)
                                                         tGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCg                                                                                              >  1:385585/1‑150 (MQ=255)
                                                          gCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGt                                                                                             >  2:912564/1‑150 (MQ=255)
                                                            tGGAATGTCCACGCTGCAATTAAGGCGAAACCTTCCTTTTTTGGGGATGAAAAATCATTGAATTGGCGCGGGTGTTCTCCCTCTCCGGGGGGGTGTGGGCGGGGGGGTGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTg                                                                                           <  1:5567/150‑1 (MQ=255)
                                                            gggCATGCCCACGCTGTAATTAAGGCTAAAACGTACCTTTTTCGGTATTAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGGGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTg                                                                                           <  2:1012597/149‑1 (MQ=38)
                                                            gggCATGCCCACGCGGGAATTAAGGCGAAAAGGTACCTTTTTCGGGATGAAAAATCAATGAAATGGCGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGGGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTg                                                                                           <  2:1202605/149‑1 (MQ=39)
                                                             ggCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGc                                                                                          >  1:411003/1‑150 (MQ=255)
                                                               cATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCCAGCGTTGCca                                                                                        >  2:465113/1‑150 (MQ=255)
                                                                 tGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCACGCGTTGCcatc                                                                                      >  2:1683463/1‑150 (MQ=255)
                                                                  gTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCcatca                                                                                     >  1:398057/1‑150 (MQ=255)
                                                                  gTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCcatca                                                                                     >  2:914796/1‑150 (MQ=255)
                                                                        gCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCg                                                                               >  1:1159779/1‑150 (MQ=255)
                                                                        gCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCAGGGGTGAGCGCACCAACTCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATAACCg                                                                               >  1:156381/1‑150 (MQ=255)
                                                                           ggAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTAGCCATCATCACCGCCt                                                                            >  1:606916/1‑150 (MQ=255)
                                                                            gAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCtt                                                                           >  2:580513/1‑150 (MQ=255)
                                                                               ttAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCATGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGCCCCAATCCCTCCCCACCCGTGGCCATCATCACCGCCTaaaa                                                                        >  2:872435/1‑149 (MQ=38)
                                                                                 aaGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCg                                                                      >  1:462236/1‑150 (MQ=255)
                                                                                   ggCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTa                                                                    >  1:985420/1‑150 (MQ=255)
                                                                                      taaaaCGTCTCTTTTTTGGTATATAAAAATCATTGATAGTGGGCGGGGGTTCCCCCGCTCGCTGGGTGCTGGGGCGGGGGTGGGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATg                                                                  <  2:704602/149‑1 (MQ=255)
                                                                                       aaaaCGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGAGTTGCCAACATCACCGCCTTAAACGTATgaa                                                                >  2:351164/1‑148 (MQ=255)
                                                                                           cGTACCTTTTTCGGGATGAAAAATCAATGAACTGGAGCCGGTGTTCTCCCTCTCCATGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCg                                                            <  2:844449/150‑1 (MQ=255)
                                                                                           cGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGCCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCTTCCGCGCCTTAAACGTATGCATCCg                                                            >  2:1247784/1‑150 (MQ=39)
                                                                                                cTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGAGGGAGAGGGCCGGGGTGAGGGCTCCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGTtttt                                                       >  1:1586122/1‑150 (MQ=255)
                                                                                                   tttCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCg                                                    >  1:1210348/1‑150 (MQ=255)
                                                                                                        ggATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGTCCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTgg                                               >  2:1364219/1‑150 (MQ=255)
                                                                                                        ggATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTgg                                               >  1:1225111/1‑150 (MQ=255)
                                                                                                         gATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGATGGCCGGGGTGAGGGCACCAACGCGCAGCCGCCCCAATCACACCCCAAGCGTTGCCATCCTCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTggg                                              >  2:1637175/1‑149 (MQ=39)
                                                                                                              aaaaaTCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGaa                                          >  1:1295578/1‑150 (MQ=255)
                                                                                                              aaaaaTCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCATAATCGTATGCATCCGGTATTCCGCCTGGTCGaa                                          >  2:392004/1‑150 (MQ=255)
                                                                                                               aaaaTCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAac                                         >  1:1717436/1‑150 (MQ=255)
                                                                                                                aaaTCAATTAAATGGCGCCGGTGTGCTCCCTCTCCCTGGGGGTGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAaca                                        <  1:991562/150‑1 (MQ=255)
                                                                                                                aaaTCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAaca                                        >  2:1025604/1‑150 (MQ=255)
                                                                                                                aaaTCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAaca                                        >  2:866006/1‑150 (MQ=255)
                                                                                                                       tGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCACGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCt                                 >  2:1175846/1‑150 (MQ=255)
                                                                                                                       tGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCt                                 >  2:968632/1‑150 (MQ=255)
                                                                                                                               aGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCCCCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGATCACGATGCTCGccgccg                         >  1:303638/1‑150 (MQ=255)
                                                                                                                                  gggTGTGCCCCCTCCCCTGGGGGGTGTGGGCGGGGGTGGGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACt                      <  2:550134/149‑1 (MQ=255)
                                                                                                                                  cGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACt                      >  1:1584104/1‑150 (MQ=255)
                                                                                                                                   gggtttCTCCCCCTACCCGGTGGGGGTGCGGGCGGGGTGGGGCCCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGa                        <  1:1453233/146‑1 (MQ=255)
                                                                                                                                     ccccggccctctcccaCAGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACGCaa                   >  2:240550/7‑150 (MQ=255)
                                                                                                                                       ccggccctctcccaCAGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGGATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCaacc                 >  2:918446/5‑148 (MQ=255)
                                                                                                                                         ctccctctccctGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCAAacac               >  1:1047745/1‑150 (MQ=255)
                                                                                                                                          gccctctcccaCAGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCAAACAcc              >  2:41653/2‑150 (MQ=255)
                                                                                                                                           ccctctccctGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCAAACAccg             >  2:335997/1‑149 (MQ=255)
                                                                                                                                               ctccttGGGGGTGGGGGCGGGGGGGGGGCCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCAAACACCTcgtc         <  2:411003/150‑1 (MQ=255)
                                                                                                                                                  ccctGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGCTGCTCGCCGCCGACTCAAACACCTcgtccg       >  2:904459/1‑150 (MQ=255)
                                                                                                                                                  ccctGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCAAACACCTcgtccg       <  2:1024563/150‑1 (MQ=255)
                                                                                                                                                   cctGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCAAACACCTcgtccgt      >  2:221807/1‑150 (MQ=255)
                                                                                                                                                       tgGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCAAACACCTCGTCCGTCAcc  <  2:997377/150‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                       
CGTTGAACAGGTCGAGGATCTGCAACGCACGTTCAACAGACTGAATAATCGGCATAATGCTGGCATGTCCACGCTGGAATTAAGGCGAAAACGTACCTTTTTCGGGATGAAAAATCAATGAAATGGAGCCGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCACCAACGCGCAGCGGACCCAATCACTCCCCAAGCGTTGCCATCATCACCGCCTTAATCGTATGCATCCGGTTTTCCGCCTGGTCGAACACGATGCTCGCCGCCGACTCAAACACCTCGTCCGTCACC  >  NC_000913/289108‑289406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: