Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,919,905 G→C 5.4% T281S (ACC→AGC)  gudX ← glucarate dehydratase‑related protein, substrate unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,919,9050GC 5.4% 320.1 / 6.8 93T281S (ACC→AGC) gudXglucarate dehydratase‑related protein, substrate unknown
Reads supporting (aligned to +/- strand):  ref base G (41/47);  new base C (3/2);  total (44/49)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

GTCGTCGCAAAGCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGTA  >  NC_000913/2919757‑2920054
                                                                                                                                                    |                                                                                                                                                     
gtcgtcGCAAAGCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTGCCGCATTGAGCATCACCGCATGACCCCTTTCGCGCCCGTTGGTGGCGATCATGTTTGTCGCGACGGGCAAGCCGGt                                                                                                                                                      >  1:830818/1‑150 (MQ=255)
gtcgtcGCAAAGCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCggg                                                                                                                                                      >  2:393679/1‑149 (MQ=255)
gtcgtcGCAAAGCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGAGCATGTTAGTCGCGACGGGCAATCCggg                                                                                                                                                      >  2:494446/1‑149 (MQ=255)
   gtcgCAAAGCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCAGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGc                                                                                                                                                   >  1:737021/1‑150 (MQ=255)
    tcgCAAAGCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGACAAGCCGGTCGcc                                                                                                                                                  <  1:374508/150‑1 (MQ=255)
    tcgCAAAGCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGGCGCGACCGGCTTGCCCGTCGcg                                                                                                                                                  <  1:1481920/150‑2 (MQ=255)
      gCAAAGCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCCCCGCATGCCCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCg                                                                                                                                                >  1:1292174/1‑150 (MQ=255)
      gCAAAGCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCg                                                                                                                                                >  1:1369107/1‑150 (MQ=255)
      gCAAAGCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACCGGCTTGCCCGTCGcgac                                                                                                                                                <  1:447285/150‑4 (MQ=255)
         aaGCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCg                                                                                                                                             <  1:709132/150‑1 (MQ=255)
           gCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCGACCGGCTTGCCCGTCgcg                                                                                                                                           <  2:1322110/150‑3 (MQ=255)
           gCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCAATTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCGACCGGCTTGCCCGTCgcg                                                                                                                                           <  1:77629/150‑3 (MQ=255)
             tGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGGGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAAt                                                                                                                                         <  1:454960/150‑1 (MQ=255)
                     acGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCAt                                                                                                                                 >  2:1716581/1‑150 (MQ=255)
                           tGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTc                                                                                                                           >  2:1198060/1‑150 (MQ=255)
                            gCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCa                                                                                                                          >  2:1515182/1‑150 (MQ=255)
                            gCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCa                                                                                                                          <  2:124898/150‑1 (MQ=255)
                            gCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCa                                                                                                                          <  2:1448051/150‑1 (MQ=255)
                              aCCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCg                                                                                                                                                <  1:1191904/126‑1 (MQ=255)
                              aCCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCg                                                                                                                                                >  2:1191904/1‑126 (MQ=255)
                              aCCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACg                                                                                                                        <  1:1503305/150‑1 (MQ=255)
                                  gAAAGCGTCCAGAAGTGCAGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCg                                                                                                                    <  2:737021/150‑1 (MQ=255)
                                   aaaGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGGTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCgg                                                                                                                   <  2:114157/150‑1 (MQ=255)
                                      gCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGgaga                                                                                                                <  1:1339116/150‑1 (MQ=255)
                                        gTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCACGCCGGTCGCCCGTCGAAATTCCGCCATCCCTTCACGTCCGGAGAAg                                                                                                              >  2:899032/1‑150 (MQ=255)
                                        gTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGATGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCCCTTCACGTCCGGAGCAg                                                                                                              >  1:117826/1‑150 (MQ=255)
                                         tcgaGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGGCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCTCTTCACGTCCGGAGAAGc                                                                                                             <  2:1129637/147‑1 (MQ=255)
                                             gAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTg                                                                                                         <  2:1491203/150‑1 (MQ=255)
                                             gAAGTGCGGAGCGGCAAGTGGAATATCTACCGCATTGAGCCTCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTg                                                                                                         <  2:290703/150‑1 (MQ=255)
                                              aaGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGt                                                                                                        <  1:1198060/150‑1 (MQ=255)
                                               aGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGtt                                                                                                       >  1:1567496/1‑150 (MQ=255)
                                                gTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTc                                                                                                      <  1:174529/150‑1 (MQ=255)
                                                gTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCACATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTc                                                                                                      <  2:1122006/150‑1 (MQ=255)
                                                    ggATCGGCAAGGGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTgcg                                                                                                  <  2:1292174/150‑1 (MQ=255)
                                                     gATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTgcgc                                                                                                 >  1:1508910/1‑150 (MQ=255)
                                                             aGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGa                                                                                         >  1:846595/1‑150 (MQ=255)
                                                                ggAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCt                                                                                      >  1:410321/1‑150 (MQ=255)
                                                                ggAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTCGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCt                                                                                      >  1:309300/1‑150 (MQ=255)
                                                                 gAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGACAGTTGGTGGCGATCATGTTAGTCGCGACGCGCAACCCCGTCGCCCCTCGCAATTCCGCCAACACATCACGTCCGGAGAAGCCCTGTTCAGCGCCGCATGGATcat                                                                                     >  1:1137503/1‑148 (MQ=255)
                                                                  aaTATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTc                                                                                    >  1:525664/1‑150 (MQ=255)
                                                                   atatCTACCGCATTGAGCATCACCGCCTGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGGTCTGCGCCGCATGGATCTTCg                                                                                   <  2:241773/150‑1 (MQ=255)
                                                                   atatCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCg                                                                                   <  1:398722/150‑1 (MQ=255)
                                                                    tatCTACCGCATTGAGCATCACCGCATGCCCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCgg                                                                                  >  1:1563209/1‑150 (MQ=255)
                                                                       cTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCAt                                                                               >  2:20992/1‑150 (MQ=255)
                                                                         aCCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAg                                                                             >  2:962006/1‑150 (MQ=255)
                                                                          ccGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAgg                                                                            <  2:642772/150‑1 (MQ=255)
                                                                            gCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGCAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTa                                                                          >  1:384185/1‑150 (MQ=255)
                                                                                tGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGaa                                                                      >  1:175044/1‑150 (MQ=255)
                                                                                     aTCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAAcatca                                                                 >  2:1115817/1‑150 (MQ=255)
                                                                                     aTCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAAcatca                                                                 >  2:610737/1‑150 (MQ=255)
                                                                                     aTCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAAcatca                                                                 <  1:1716581/150‑1 (MQ=255)
                                                                                         ccGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACatcaatca                                                             <  1:1538660/150‑1 (MQ=255)
                                                                                          cGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAg                                                            <  1:393679/150‑1 (MQ=255)
                                                                                           gCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGc                                                           <  1:513990/150‑1 (MQ=255)
                                                                                            cATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGcc                                                          >  1:1364399/1‑150 (MQ=255)
                                                                                            cATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGcc                                                          >  1:124420/1‑150 (MQ=255)
                                                                                                   cATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCCGCCCTTTGCa                                                   >  2:1036084/1‑150 (MQ=255)
                                                                                                   cATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCa                                                   >  2:1611608/1‑150 (MQ=255)
                                                                                                   cATTTCGCGCCAGTTGGTGGAGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCa                                                   <  1:1348180/150‑1 (MQ=255)
                                                                                                      ttCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATaa                                                <  1:1396446/150‑1 (MQ=255)
                                                                                                      ttCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATaa                                                <  1:398149/150‑1 (MQ=255)
                                                                                                       tCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATaaa                                               <  2:1633804/150‑1 (MQ=255)
                                                                                                       tCGCGCCAGGTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATaaa                                               <  2:380662/150‑1 (MQ=255)
                                                                                                         gcgcCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGa                                             <  1:1116027/150‑1 (MQ=255)
                                                                                                         gcgcCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGa                                             >  1:885427/1‑150 (MQ=255)
                                                                                                          cgcCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCAATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGAGCTTCGGCATAGGTAAGCACATCATTCCGCCCTTTGCATAAAGaa                                            >  2:297717/1‑150 (MQ=255)
                                                                                                               gTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGc                                       >  2:1656243/1‑150 (MQ=255)
                                                                                                                 tggtggCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCtt                                     <  1:1115817/150‑1 (MQ=255)
                                                                                                                  ggtggCGATCATGTTAGTCGCGACTGGCAAGCCGGTGGCCCGTCGAAATTCGGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTtc                                    <  2:1137503/150‑1 (MQ=255)
                                                                                                                    tggCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTtcat                                  <  2:410321/150‑1 (MQ=255)
                                                                                                                       cGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCaa                               >  2:909930/1‑150 (MQ=255)
                                                                                                                        gATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAag                              >  1:473348/1‑150 (MQ=255)
                                                                                                                          tCATGTTAGTCGCGACGGGCAAGCCGGTCTCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAagca                            <  1:67954/150‑1 (MQ=255)
                                                                                                                             tGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGcc                         <  2:880084/150‑1 (MQ=255)
                                                                                                                              gTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCa                        >  1:409644/1‑150 (MQ=255)
                                                                                                                              gTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCa                        <  1:962006/150‑1 (MQ=255)
                                                                                                                                tAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATg                      <  1:494446/150‑1 (MQ=255)
                                                                                                                                 aGTCGCGTCGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCGTTTTCAGCGCCTCATGGATCTTCGGCTTAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGc                     <  1:1047490/150‑1 (MQ=255)
                                                                                                                                 aGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGc                     <  1:20992/150‑1 (MQ=255)
                                                                                                                                  gTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCCTCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCa                    >  1:1305951/1‑150 (MQ=255)
                                                                                                                                   tCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCAc                   <  1:1607368/150‑1 (MQ=255)
                                                                                                                                    ttcgACGGGCGACCGGCTTGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGCACATCATTCAGCACTTTGCATAAAGAAATGGCTTCATGAAGCAGCCATGCAcc                  >  1:854754/3‑150 (MQ=255)
                                                                                                                                    ttcgACGGGCGACCGGCTTGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTAAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCAcc                  >  2:1040093/3‑150 (MQ=255)
                                                                                                                                    cgcgACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTTTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCAcc                  <  1:1652054/150‑1 (MQ=255)
                                                                                                                                    cgcgACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCAcc                  <  1:9531/150‑1 (MQ=255)
                                                                                                                                     tcgACGGGCGACCGGCTTGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCg                 >  1:494413/2‑150 (MQ=255)
                                                                                                                                     gcgACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCg                 <  2:473348/150‑1 (MQ=255)
                                                                                                                                      cgACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGt                >  2:1288472/1‑150 (MQ=255)
                                                                                                                                      cgACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGt                >  2:1040974/1‑150 (MQ=255)
                                                                                                                                          gggCAAGCCGGGCGCCCGTCGAAATTCCGCCATCCCTTCACGTCCGGAGACGCCCTGTTCTGCGCCGCATGGATCTTCGGCATTGGGAGAACATCATTCAGCCCTTTGCAAAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGa           <  2:830818/150‑1 (MQ=255)
                                                                                                                                             cAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATc         >  1:707625/1‑150 (MQ=255)
                                                                                                                                               aGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCaa       >  1:957033/1‑150 (MQ=255)
                                                                                                                                               aGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCAGAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCaa       >  1:918997/1‑150 (MQ=255)
                                                                                                                                                gCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAAc      <  1:1036084/150‑1 (MQ=255)
                                                                                                                                                  cGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACgg    >  1:955484/1‑150 (MQ=255)
                                                                                                                                                   ggTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGt   <  2:1567496/150‑1 (MQ=255)
                                                                                                                                                    gTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTGATCAAGCAGCCATGCACCGTTGGGATCAACGGTa  <  2:309300/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GTCGTCGCAAAGCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGTA  >  NC_000913/2919757‑2920054

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: