Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,919,907 C→T 5.3% A280A (GCG→GCA gudX ← glucarate dehydratase‑related protein, substrate unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,919,9070CT 5.3% 303.7 / 2.2 94A280A (GCG→GCAgudXglucarate dehydratase‑related protein, substrate unknown
Reads supporting (aligned to +/- strand):  ref base C (40/48);  new base T (3/2);  total (43/51)
Fisher's exact test for biased strand distribution p-value = 6.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCGCAAAGCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGTAAT  >  NC_000913/2919760‑2920056
                                                                                                                                                   |                                                                                                                                                     
gtcgCAAAGCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCAGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGc                                                                                                                                                     >  1:737021/1‑150 (MQ=255)
 tcgCAAAGCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGACAAGCCGGTCGcc                                                                                                                                                    <  1:374508/150‑1 (MQ=255)
 tcgCAAAGCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGGCGCGACCGGCTTGCCCGTCGcg                                                                                                                                                    <  1:1481920/150‑2 (MQ=255)
   gCAAAGCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCCCCGCATGCCCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCg                                                                                                                                                  >  1:1292174/1‑150 (MQ=255)
   gCAAAGCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCg                                                                                                                                                  >  1:1369107/1‑150 (MQ=255)
   gCAAAGCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACCGGCTTGCCCGTCGcgac                                                                                                                                                  <  1:447285/150‑4 (MQ=255)
      aaGCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCg                                                                                                                                               <  1:709132/150‑1 (MQ=255)
        gCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCGACCGGCTTGCCCGTCgcg                                                                                                                                             <  2:1322110/150‑3 (MQ=255)
        gCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCAATTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCGACCGGCTTGCCCGTCgcg                                                                                                                                             <  1:77629/150‑3 (MQ=255)
          tGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGGGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAAt                                                                                                                                           <  1:454960/150‑1 (MQ=255)
                  acGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCAt                                                                                                                                   >  2:1716581/1‑150 (MQ=255)
                        tGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTc                                                                                                                             >  2:1198060/1‑150 (MQ=255)
                         gCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCa                                                                                                                            <  2:124898/150‑1 (MQ=255)
                         gCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCa                                                                                                                            <  2:1448051/150‑1 (MQ=255)
                         gCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCa                                                                                                                            >  2:1515182/1‑150 (MQ=255)
                           aCCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCg                                                                                                                                                  >  2:1191904/1‑126 (MQ=255)
                           aCCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCg                                                                                                                                                  <  1:1191904/126‑1 (MQ=255)
                           aCCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACg                                                                                                                          <  1:1503305/150‑1 (MQ=255)
                               gAAAGCGTCCAGAAGTGCAGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCg                                                                                                                      <  2:737021/150‑1 (MQ=255)
                                aaaGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGGTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCgg                                                                                                                     <  2:114157/150‑1 (MQ=255)
                                   gCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGgaga                                                                                                                  <  1:1339116/150‑1 (MQ=255)
                                     gTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCACGCCGGTCGCCCGTCGAAATTCCGCCATCCCTTCACGTCCGGAGAAg                                                                                                                >  2:899032/1‑150 (MQ=255)
                                     gTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGATGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCCCTTCACGTCCGGAGCAg                                                                                                                >  1:117826/1‑150 (MQ=255)
                                      tcgaGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGGCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCTCTTCACGTCCGGAGAAGc                                                                                                               <  2:1129637/147‑1 (MQ=255)
                                          gAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTg                                                                                                           <  2:1491203/150‑1 (MQ=255)
                                          gAAGTGCGGAGCGGCAAGTGGAATATCTACCGCATTGAGCCTCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTg                                                                                                           <  2:290703/150‑1 (MQ=255)
                                           aaGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGt                                                                                                          <  1:1198060/150‑1 (MQ=255)
                                            aGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGtt                                                                                                         >  1:1567496/1‑150 (MQ=255)
                                             gTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTc                                                                                                        <  1:174529/150‑1 (MQ=255)
                                             gTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCACATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTc                                                                                                        <  2:1122006/150‑1 (MQ=255)
                                                 ggATCGGCAAGGGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTgcg                                                                                                    <  2:1292174/150‑1 (MQ=255)
                                                  gATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTgcgc                                                                                                   >  1:1508910/1‑150 (MQ=255)
                                                          aGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGa                                                                                           >  1:846595/1‑150 (MQ=255)
                                                             ggAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCt                                                                                        >  1:410321/1‑150 (MQ=255)
                                                             ggAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTCGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCt                                                                                        >  1:309300/1‑150 (MQ=255)
                                                              gAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGACAGTTGGTGGCGATCATGTTAGTCGCGACGCGCAACCCCGTCGCCCCTCGCAATTCCGCCAACACATCACGTCCGGAGAAGCCCTGTTCAGCGCCGCATGGATcat                                                                                       >  1:1137503/1‑148 (MQ=255)
                                                               aaTATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTc                                                                                      >  1:525664/1‑150 (MQ=255)
                                                                atatCTACCGCATTGAGCATCACCGCCTGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGGTCTGCGCCGCATGGATCTTCg                                                                                     <  2:241773/150‑1 (MQ=255)
                                                                atatCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCg                                                                                     <  1:398722/150‑1 (MQ=255)
                                                                 tatCTACCGCATTGAGCATCACCGCATGCCCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCgg                                                                                    >  1:1563209/1‑150 (MQ=255)
                                                                    cTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCAt                                                                                 >  2:20992/1‑150 (MQ=255)
                                                                      aCCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAg                                                                               >  2:962006/1‑150 (MQ=255)
                                                                       ccGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAgg                                                                              <  2:642772/150‑1 (MQ=255)
                                                                         gCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGCAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTa                                                                            >  1:384185/1‑150 (MQ=255)
                                                                             tGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGaa                                                                        >  1:175044/1‑150 (MQ=255)
                                                                                  aTCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAAcatca                                                                   >  2:1115817/1‑150 (MQ=255)
                                                                                  aTCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAAcatca                                                                   <  1:1716581/150‑1 (MQ=255)
                                                                                  aTCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAAcatca                                                                   >  2:610737/1‑150 (MQ=255)
                                                                                      ccGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACatcaatca                                                               <  1:1538660/150‑1 (MQ=255)
                                                                                       cGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAg                                                              <  1:393679/150‑1 (MQ=255)
                                                                                        gCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGc                                                             <  1:513990/150‑1 (MQ=255)
                                                                                         cATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGcc                                                            >  1:124420/1‑150 (MQ=255)
                                                                                         cATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGcc                                                            >  1:1364399/1‑150 (MQ=255)
                                                                                                cATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCCGCCCTTTGCa                                                     >  2:1036084/1‑150 (MQ=255)
                                                                                                cATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCa                                                     >  2:1611608/1‑150 (MQ=255)
                                                                                                cATTTCGCGCCAGTTGGTGGAGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCa                                                     <  1:1348180/150‑1 (MQ=255)
                                                                                                   ttCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATaa                                                  <  1:398149/150‑1 (MQ=255)
                                                                                                   ttCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATaa                                                  <  1:1396446/150‑1 (MQ=255)
                                                                                                    tCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATaaa                                                 <  2:1633804/150‑1 (MQ=255)
                                                                                                    tCGCGCCAGGTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATaaa                                                 <  2:380662/150‑1 (MQ=255)
                                                                                                      gcgcCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGa                                               <  1:1116027/150‑1 (MQ=255)
                                                                                                      gcgcCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGa                                               >  1:885427/1‑150 (MQ=255)
                                                                                                       cgcCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCAATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGAGCTTCGGCATAGGTAAGCACATCATTCCGCCCTTTGCATAAAGaa                                              >  2:297717/1‑150 (MQ=255)
                                                                                                            gTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGc                                         >  2:1656243/1‑150 (MQ=255)
                                                                                                              tggtggCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCtt                                       <  1:1115817/150‑1 (MQ=255)
                                                                                                               ggtggCGATCATGTTAGTCGCGACTGGCAAGCCGGTGGCCCGTCGAAATTCGGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTtc                                      <  2:1137503/150‑1 (MQ=255)
                                                                                                                 tggCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTtcat                                    <  2:410321/150‑1 (MQ=255)
                                                                                                                    cGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCaa                                 >  2:909930/1‑150 (MQ=255)
                                                                                                                     gATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAag                                >  1:473348/1‑150 (MQ=255)
                                                                                                                       tCATGTTAGTCGCGACGGGCAAGCCGGTCTCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAagca                              <  1:67954/150‑1 (MQ=255)
                                                                                                                          tGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGcc                           <  2:880084/150‑1 (MQ=255)
                                                                                                                           gTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCa                          >  1:409644/1‑150 (MQ=255)
                                                                                                                           gTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCa                          <  1:962006/150‑1 (MQ=255)
                                                                                                                             tAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATg                        <  1:494446/150‑1 (MQ=255)
                                                                                                                              aGTCGCGTCGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCGTTTTCAGCGCCTCATGGATCTTCGGCTTAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGc                       <  1:1047490/150‑1 (MQ=255)
                                                                                                                              aGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGc                       <  1:20992/150‑1 (MQ=255)
                                                                                                                               gTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCCTCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCa                      >  1:1305951/1‑150 (MQ=255)
                                                                                                                                tCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCAc                     <  1:1607368/150‑1 (MQ=255)
                                                                                                                                 ttcgACGGGCGACCGGCTTGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGCACATCATTCAGCACTTTGCATAAAGAAATGGCTTCATGAAGCAGCCATGCAcc                    >  1:854754/3‑150 (MQ=255)
                                                                                                                                 ttcgACGGGCGACCGGCTTGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTAAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCAcc                    >  2:1040093/3‑150 (MQ=255)
                                                                                                                                 cgcgACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTTTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCAcc                    <  1:1652054/150‑1 (MQ=255)
                                                                                                                                 cgcgACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCAcc                    <  1:9531/150‑1 (MQ=255)
                                                                                                                                  tcgACGGGCGACCGGCTTGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCg                   >  1:494413/2‑150 (MQ=255)
                                                                                                                                  gcgACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCg                   <  2:473348/150‑1 (MQ=255)
                                                                                                                                   cgACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGt                  >  2:1288472/1‑150 (MQ=255)
                                                                                                                                   cgACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGt                  >  2:1040974/1‑150 (MQ=255)
                                                                                                                                       gggCAAGCCGGGCGCCCGTCGAAATTCCGCCATCCCTTCACGTCCGGAGACGCCCTGTTCTGCGCCGCATGGATCTTCGGCATTGGGAGAACATCATTCAGCCCTTTGCAAAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGa             <  2:830818/150‑1 (MQ=255)
                                                                                                                                          cAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATc           >  1:707625/1‑150 (MQ=255)
                                                                                                                                            aGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCaa         >  1:957033/1‑150 (MQ=255)
                                                                                                                                            aGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCAGAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCaa         >  1:918997/1‑150 (MQ=255)
                                                                                                                                             gCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAAc        <  1:1036084/150‑1 (MQ=255)
                                                                                                                                               cGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACgg      >  1:955484/1‑150 (MQ=255)
                                                                                                                                                ggTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGt     <  2:1567496/150‑1 (MQ=255)
                                                                                                                                                 gTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTGATCAAGCAGCCATGCACCGTTGGGATCAACGGTa    <  2:309300/150‑1 (MQ=255)
                                                                                                                                                   cGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGTAAt  >  2:65244/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GTCGCAAAGCTGCGCCACACGGACTGCACCGGAAAGCGTCCAGAAGTGCGGATCGGCAAGTGGAATATCTACCGCATTGAGCATCACCGCATGACCCATTTCGCGCCAGTTGGTGGCGATCATGTTAGTCGCGACGGGCAAGCCGGTCGCCCGTCGAAATTCCGCCATCACTTCACGTCCGGAGAAGCCCTGTTCTGCGCCGCATGGATCTTCGGCATAGGTAAGAACATCATTCAGCCCTTTGCATAAAGAAATGGCTTCATCAAGCAGCCATGCACCGTTGGGATCAACGGTAAT  >  NC_000913/2919760‑2920056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: