BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	297,212	C→A	5.7%	pseudogene (7/49 nt)	ptwF →	tRNA‑OTHER

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	297,212	0	C	A	5.7%	412.7 / 10.0	135	pseudogene (7/49 nt)	ptwF	tRNA‑OTHER
Reads supporting (aligned to +/- strand): ref base C (69/57); new base A (4/4); total (74/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.95e-01

Only 100 of 136 total aligned reads displayed.

TGCTGGTGTAAGTAAATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATACTGTTCTGACATCAG  >  NC_000913/297065‑297361

tgctgGTGTAAGTAAATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATAAAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAAAGCATTAACATCGGATTCGt                                                                                                                                                     >  1:78456/1‑150 (MQ=255)

  ctgGTGTAAGTAAATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTaa                                                                                                                                                   <  1:163182/150‑1 (MQ=255)

    ggTGTAAGTAAATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATg                                                                                                                                                 >  2:343343/1‑150 (MQ=255)

      tgtAAGTAAATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCg                                                                                                                                               >  1:495620/1‑150 (MQ=255)

        taagtaaATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTACGAATGCGat                                                                                                                                             <  1:653379/150‑2 (MQ=255)

         aagtaaATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTACGAATGCGatg                                                                                                                                            <  1:1156357/150‑3 (MQ=255)

          agtaaATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAgg                                                                                                                                           >  1:1024459/1‑150 (MQ=255)

          agtaaATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAgg                                                                                                                                           <  1:795715/150‑1 (MQ=255)

           gtaaATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGTTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTACGAATGCGatgtt                                                                                                                                          <  2:263753/150‑5 (MQ=255)

               aTGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTa                                                                                                                                      <  1:1531004/150‑1 (MQ=255)

                  gAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGt                                                                                                                                   >  1:467240/1‑150 (MQ=255)

                   aGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGtt                                                                                                                                  <  1:1114012/150‑1 (MQ=255)

                   aGGGAATAAAAATCAACTTTTGTTAATTTCAATCAACGGGTTTTTTGCTATAACTGGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGtt                                                                                                                                  <  1:840779/150‑1 (MQ=255)

                    gtgAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTc                                                                                                                                 <  2:1346094/148‑1 (MQ=255)

                      gattAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGa                                                                                                                               <  2:330896/147‑1 (MQ=255)

                      gAATAACCATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGa                                                                                                                               >  1:1579871/1‑150 (MQ=255)

                      gAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGa                                                                                                                               >  2:372711/1‑150 (MQ=255)

                      gAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGa                                                                                                                               >  1:1586290/1‑150 (MQ=255)

                             cATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCtat                                                                                                                        <  1:737098/150‑1 (MQ=255)

                             cATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCtat                                                                                                                        >  2:474532/1‑150 (MQ=255)

                              aTCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCtatt                                                                                                                       >  1:1415324/1‑150 (MQ=255)

                              aTCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCtatt                                                                                                                       >  2:480481/1‑150 (MQ=255)

                                       tGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGTGAAGGTCGTAGGTTCGACTCCTATTATCGGCAcc                                                                                                              <  1:1571368/150‑1 (MQ=255)

                                        gTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATTTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCTCATTCGTAATGCGAATGTCGTAGGTTCGACTCCTATTATCGGCACCa                                                                                                             >  1:1040327/1‑150 (MQ=255)

                                        gTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTTGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCa                                                                                                             >  2:1479019/1‑150 (MQ=255)

                                            aTTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTaa                                                                                                         >  2:636860/1‑150 (MQ=255)

                                              ttCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTaaaa                                                                                                       >  2:1366460/1‑150 (MQ=255)

                                                 aatcaaCGGGTTTATTGCTATAACTTGGTTTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCa                                                                                                    <  1:1623311/150‑1 (MQ=255)

                                                 aatcaaCGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAATTGAATTAACATCGCATTCGTAATGCGAAGGTAGTAGGTTCGACTCCTATTATCGGCACCATTAAAAtaa                                                                                                    >  1:712047/1‑148 (MQ=255)

                                                  atcaaCGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCaa                                                                                                   <  2:1024459/150‑1 (MQ=255)

                                                    caaCGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAag                                                                                                 <  2:131851/150‑1 (MQ=255)

                                                       cGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGt                                                                                              >  2:446131/1‑150 (MQ=255)

                                                         ggTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATTAATAAACATCTCATTCGTAATGCGAAGGTCGTAGGTTCGACTCATATTATCGGCACCATTAAAATCAAAAAGTTa                                                                                            >  1:1470322/1‑150 (MQ=37)

                                                         ggTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTa                                                                                            >  2:1065123/1‑150 (MQ=255)

                                                             tATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTAcccc                                                                                        >  1:966726/1‑150 (MQ=255)

                                                                   tataACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATAttt                                                                                  >  1:575967/1‑150 (MQ=255)

                                                                    ataACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTa                                                                                 >  2:1158925/1‑150 (MQ=255)

                                                                    ataACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTa                                                                                 >  2:1294051/1‑150 (MQ=255)

                                                                     taACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGAGAAGGTCGTAGGTTCGAATCATATTATCTGCACCATTAAAATCAAAGAGTTAACCCATATTTaa                                                                                >  1:286636/1‑150 (MQ=255)

                                                                      aaCTTGGGGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAACTGCATAAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTaaa                                                                               <  2:885815/150‑1 (MQ=255)

                                                                         ttGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATac                                                                            >  1:396436/1‑150 (MQ=255)

                                                                          tGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTAccc                                                                                         >  1:1678635/1‑136 (MQ=255)

                                                                          tGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTAccc                                                                                         <  2:1678635/136‑1 (MQ=255)

                                                                          tGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTA‑TATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATacac                                                                          <  2:1586290/150‑1 (MQ=255)

                                                                          tGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTA‑TATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATacac                                                                          <  2:1579871/150‑1 (MQ=255)

                                                                            gtgtTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATAcacc                                                                         >  2:882397/1‑150 (MQ=255)

                                                                               ttttATAATATAAAGTATGTTGTTTTTATTGATTTCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACg                                                                      <  2:1486971/150‑1 (MQ=255)

                                                                                   ataatataaAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTtc                                                                  <  2:618006/150‑1 (MQ=255)

                                                                                   ataatataaAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTtc                                                                  <  1:1158925/150‑1 (MQ=255)

                                                                                    taatataaAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTtcc                                                                 >  1:1699127/1‑150 (MQ=255)

                                                                                    taatataaAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTtcc                                                                 >  2:1021227/1‑150 (MQ=255)

                                                                                     aatataaAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCata                                                                                     >  2:74230/1‑129 (MQ=255)

                                                                                     aatataaAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCata                                                                                     <  1:74230/129‑1 (MQ=255)

                                                                                       tataaAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTtcctcc                                                              <  2:1471388/150‑1 (MQ=255)

                                                                                        ataaAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTcctcct                                                             >  1:199902/1‑150 (MQ=255)

                                                                                         taaAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACCCCACGTTTCCTCCtg                                                            >  1:1491376/1‑150 (MQ=255)

                                                                                         taaAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCtg                                                            >  2:813127/1‑150 (MQ=255)

                                                                                           aaGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCtgtg                                                          >  2:843552/1‑150 (MQ=255)

                                                                                              tATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCg                                                       <  1:882397/150‑1 (MQ=255)

                                                                                              tATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCg                                                       <  2:457079/150‑1 (MQ=255)

                                                                                              tATGTTGTTGTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCTTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCg                                                       <  1:118823/150‑1 (MQ=255)

                                                                                                tgttgtTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTtcct                                                                >  1:860878/1‑139 (MQ=255)

                                                                                                tgttgtTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTtcct                                                                <  2:860878/139‑1 (MQ=255)

                                                                                                    gttTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACACCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTtg                                                 >  1:654427/1‑150 (MQ=255)

                                                                                                       ttgattgaTTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGc                                              <  1:1065123/150‑1 (MQ=255)

                                                                                                        tgattgatTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGcc                                             >  2:85968/1‑150 (MQ=255)

                                                                                                         gattgattGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCa                                            >  1:1598088/1‑150 (MQ=255)

                                                                                                         gattgattGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCa                                            <  2:378371/150‑1 (MQ=255)

                                                                                                          tttgattgCTCAAGTAGTTAAATATGCATTAACATGGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACGACGTTTCCTCCTGTGCCGTATTTGTGCCAt                                           <  2:1131506/149‑1 (MQ=255)

                                                                                                           ttgattgCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGACGTATTTGTGCCAtt                                          <  2:869630/150‑1 (MQ=255)

                                                                                                             tattgCTCATGTAGTTAAAAATGCATTAACATCTCTTTCGTAATGCGAAGGTCGTAGGTTCGACTACTATTATCGTCACCATTAAAATCAAAGAGTTACCCGATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGt                                        <  1:254838/149‑1 (MQ=38)

                                                                                                             gattgCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGt                                        >  2:116554/1‑150 (MQ=255)

                                                                                                             gattgCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGt                                        >  2:433616/1‑150 (MQ=255)

                                                                                                              attgCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTa                                       <  1:576691/150‑1 (MQ=255)

                                                                                                                   tCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCtt                                  <  1:446131/150‑1 (MQ=255)

                                                                                                                    ctagtagtTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTg                                 <  1:1540996/148‑1 (MQ=255)

                                                                                                                    cAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTg                                 >  1:400794/1‑150 (MQ=255)

                                                                                                                         agtTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAAt                            >  1:539173/1‑150 (MQ=255)

                                                                                                                         agtTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTAACGGCACCATTAAAATAAAAGAGTTACCCAATATTTAAATACACAACGTTTCCTCCGGTGCCGTATTTGTGCCATTGTAACCTTGGCAAt                            >  1:939519/1‑150 (MQ=38)

                                                                                                                          gtTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACACCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAAtt                           >  1:562229/1‑150 (MQ=255)

                                                                                                                              aaaaTGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATtcatc                       >  1:338325/1‑150 (MQ=255)

                                                                                                                              aaaaTGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATtcatc                       >  1:1110614/1‑150 (MQ=255)

                                                                                                                                aaTGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCaa                     >  1:1286167/1‑150 (MQ=255)

                                                                                                                                aaTGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCaa                     <  1:372711/150‑1 (MQ=255)

                                                                                                                                   gCATTAACATCGCATTCGTAATGCGAAGGTCGTATGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAAt                  <  2:319347/150‑1 (MQ=255)

                                                                                                                                   gCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAAt                  <  1:433616/150‑1 (MQ=255)

                                                                                                                                   gCATTAACATCGCATTAGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCGTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAAt                  <  2:712047/150‑1 (MQ=255)

                                                                                                                                    cATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATa                 >  2:1582566/1‑150 (MQ=255)

                                                                                                                                     aTTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATAc                >  1:1341114/1‑150 (MQ=255)

                                                                                                                                     aTTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATAc                <  1:843552/150‑1 (MQ=255)

                                                                                                                                      ttAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATACt               >  2:1613596/1‑150 (MQ=255)

                                                                                                                                       tAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATACTg              <  1:190422/150‑1 (MQ=255)

                                                                                                                                        accttCGCATTACGAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATACTGt             >  1:735467/5‑150 (MQ=255)

                                                                                                                                        aaCATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATACTGt             <  1:1021227/150‑1 (MQ=255)

                                                                                                                                         ccttCGCATTACGAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATACTGtt            >  2:1232048/4‑150 (MQ=255)

                                                                                                                                          cATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACTCCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATACTGTTc           >  2:117916/1‑150 (MQ=255)

                                                                                                                                          cATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATAAAAATCAAAGAGTTACCCCAAATTTAAATACACCACGTTTCCTCATGTTCCGTATTTGTGCCATAGTAACCTTGGCAATTCATCAAAATACAGTTc           >  1:1720706/1‑150 (MQ=37)

                                                                                                                                           ttCGCATTACGAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCAAGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATACTGTTCt          >  2:1212189/2‑150 (MQ=255)

                                                                                                                                           aTCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATACTGTTCt          <  2:1286167/150‑1 (MQ=255)

                                                                                                                                                   cGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTACCCTTGGCAATTCATCAAAATAATGTTCTGCCATCAg  >  1:206449/1‑150 (MQ=255)

TGCTGGTGTAAGTAAATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATACTGTTCTGACATCAG  >  NC_000913/297065‑297361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`