BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,962,963	G→C	6.6%	T65R (ACG→AGG) ‡	ygdB ←	DUF2509 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,962,963	0	G	C	6.6%	355.3 / 12.5	106	T65R (ACG→AGG) ‡	ygdB	DUF2509 family protein
Reads supporting (aligned to +/- strand): ref base G (46/53); new base C (4/3); total (50/56)
Fisher's exact test for biased strand distribution p-value = 7.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01

Only 100 of 107 total aligned reads displayed.

AAACAATGTTTCCATCAATGACTTCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACCAGCAG  >  NC_000913/2962814‑2963111

aaaCAATGTATCCAACAATGACTTCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAAACAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCg                                                                                                                                                      <  2:1041874/150‑1 (MQ=255)

 aaCAATGTTTCCATCAATGACTTCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGt                                                                                                                                                     >  2:182988/1‑150 (MQ=255)

   cAATGTTTCCATCAATGACTTCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCt                                                                                                                                                   >  1:1553701/1‑150 (MQ=255)

   cAATGTTTCCATCAATGACTTCGCCTGTTCGCCACAACGAAAAGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCt                                                                                                                                                   <  1:1514277/150‑1 (MQ=255)

         ttCCATCAATGACTTCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCcagc                                                                                                                                             >  1:915292/1‑150 (MQ=255)

           ccATCAATGACTTCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGTTGGCTGCGTCTGCcagcag                                                                                                                                           <  2:546295/150‑1 (MQ=255)

           ccATCAATGACTTCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCcagcag                                                                                                                                           >  1:874308/1‑150 (MQ=255)

           ccATCAATGACTTCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCcagcag                                                                                                                                           >  1:996811/1‑150 (MQ=255)

           ccATCAATGACTTCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCAGACGCAGCcagcag                                                                                                                                           <  1:352038/150‑1 (MQ=255)

            cctCAAAGACTTCGCCGGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCAGACGCAGCCAGCAGt                                                                                                                                          <  2:1117549/148‑1 (MQ=255)

            cATCAATGACTTCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCAGACGCAGCCAGCAGt                                                                                                                                          <  2:910489/150‑1 (MQ=255)

                aaTGACTTCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCAt                                                                                                                                      >  1:1294068/1‑150 (MQ=255)

                   gACTTCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCAtttt                                                                                                                                   <  2:1298472/150‑1 (MQ=255)

                    aCTTCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCAttttt                                                                                                                                  >  1:107308/1‑150 (MQ=255)

                      ttCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTcc                                                                                                                                <  2:1006146/150‑1 (MQ=255)

                      ttCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAAACTGGGCATCGGTTTCAGAGTACTGCCAGCTCTGAACTGCTGGCTGCGTCTGCCAGCATTGCATTTTTcc                                                                                                                                >  2:355380/1‑150 (MQ=255)

                       tCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGGAATCAATAAGGCTTCATTATCTGCCTGTAATCGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTccc                                                                                                                               <  2:1543138/150‑1 (MQ=255)

                       tCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTccc                                                                                                                               <  2:964172/150‑1 (MQ=255)

                       tCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTccc                                                                                                                               <  2:706553/150‑1 (MQ=255)

                        cGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTcccc                                                                                                                              <  2:1295558/150‑1 (MQ=255)

                        cGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTAACTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTcccc                                                                                                                              <  2:253905/150‑1 (MQ=255)

                          ccTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCAc                                                                                                                            >  1:661390/1‑150 (MQ=255)

                               tCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAg                                                                                                                       <  1:885109/150‑1 (MQ=255)

                                cGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAgc                                                                                                                      >  2:398421/1‑150 (MQ=255)

                                cGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCGGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAgc                                                                                                                      <  2:73579/150‑1 (MQ=255)

                                  ccACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTACACGCACACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGACTGCAAGCAGTGCATTTTTCCCCACGCCAgcgc                                                                                                                    >  1:256285/1‑150 (MQ=255)

                                  ccACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAgcgc                                                                                                                    >  1:283440/1‑150 (MQ=255)

                                    acaACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCg                                                                                                                  <  1:226375/150‑1 (MQ=255)

                                      aaCGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGCTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGAc                                                                                                                <  2:584556/150‑1 (MQ=255)

                                           aaCGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAAc                                                                                                           <  2:915292/150‑1 (MQ=255)

                                             cGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGa                                                                                                         >  1:1145308/1‑150 (MQ=255)

                                             cGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCCCGCCAGCGCCGACTGAACGAt                                                                                                        >  1:1053813/1‑150 (MQ=255)

                                               ccTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATg                                                                                                       >  1:843688/1‑150 (MQ=255)

                                               ccTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATg                                                                                                       <  2:576467/150‑1 (MQ=255)

                                                cTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGTCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATgg                                                                                                      <  1:1483512/150‑1 (MQ=255)

                                                  tCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGcc                                                                                                    <  1:797468/150‑1 (MQ=255)

                                                   cATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCt                                                                                                   >  2:1482881/1‑150 (MQ=255)

                                                      aaCCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTggc                                                                                                >  1:561228/1‑150 (MQ=255)

                                                         cGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTggcggc                                                                                             >  1:1450620/1‑150 (MQ=255)

                                                          ggCAAGCAATCAGGCAGCACTCGCTCCCACTAAACGCCAACAAACCTGCGCAGCGGTTTCAGCGTACTGCGAGCACGGAACTGCTGGGGGCGTGTGCCAGCAGTGCATTTTTCCACACGCCAGCGCCGACTGAACGATGGCCTGgcggcg                                                                                            <  2:308271/150‑1 (MQ=255)

                                                            caatcaatAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCa                                                                                          <  2:189290/150‑1 (MQ=255)

                                                             aatcaatAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCaa                                                                                         >  2:1505606/1‑150 (MQ=255)

                                                              atcaatAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAAt                                                                                        >  1:1674839/1‑150 (MQ=255)

                                                              atcaatAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAAt                                                                                        >  1:1244569/1‑150 (MQ=255)

                                                                 aatAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAAtgac                                                                                     >  1:459852/1‑150 (MQ=255)

                                                                  atAATGCTTCATTCACTGCCAGTACACGCAAACTAACCTGGGCTTCGGTTGCAGCGTACTGCGAGCACGGAACTGCTGGCTGCGGCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAAtgact                                                                                    <  1:381333/150‑1 (MQ=255)

                                                                   tAAGGCTTCATTATCTGCCAGTAACCGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAAtgactg                                                                                   <  1:1622351/150‑1 (MQ=255)

                                                                     aGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAAtgactgac                                                                                 >  1:930035/1‑150 (MQ=255)

                                                                     aGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAAtgactgac                                                                                 >  2:1199177/1‑150 (MQ=255)

                                                                     aGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGGTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAAtgactgac                                                                                 <  2:1402154/150‑1 (MQ=255)

                                                                      ggCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATg                                                                                                       <  1:1461011/127‑1 (MQ=255)

                                                                      ggCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATg                                                                                                       >  2:1461011/1‑127 (MQ=255)

                                                                             ttATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATg                                                                         <  1:1343419/150‑1 (MQ=255)

                                                                                  tGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGCACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCAc                                                                    >  1:502303/1‑150 (MQ=255)

                                                                                   gCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCAcg                                                                   <  1:182988/150‑1 (MQ=255)

                                                                                     cAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCAcgcg                                                                 <  1:1482881/150‑1 (MQ=255)

                                                                                          aaCGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAgg                                                            <  2:120244/150‑1 (MQ=255)

                                                                                           aCGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTT‑CATGCTCACGCGAGAGGCa                                                          <  2:1450620/150‑1 (MQ=255)

                                                                                            cGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCa                                                          <  2:283440/150‑1 (MQ=255)

                                                                                             gCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCaa                                                         >  2:1626731/1‑150 (MQ=255)

                                                                                              caaacaaaCCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCAt                                                                          >  1:985883/1‑132 (MQ=255)

                                                                                                aacaaacCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAAc                                                      <  2:1145308/150‑1 (MQ=255)

                                                                                                   aaacCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCGGCGGCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGc                                                   <  2:1445679/150‑1 (MQ=255)

                                                                                                      ccTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGAt                                                <  2:843688/150‑1 (MQ=255)

                                                                                                          ggCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTg                                            >  1:570983/1‑150 (MQ=255)

                                                                                                            cATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGtt                                          <  2:1278748/150‑1 (MQ=255)

                                                                                                             aTCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTg                                         <  2:661390/150‑1 (MQ=255)

                                                                                                             aTCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTg                                         >  2:1403799/1‑150 (MQ=255)

                                                                                                             aTCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTg                                         <  2:152890/150‑1 (MQ=255)

                                                                                                               cGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGc                                       <  2:429044/150‑1 (MQ=255)

                                                                                                                  tttCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCa                                    >  1:1700194/1‑150 (MQ=255)

                                                                                                                    tCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCAtt                                  >  1:354998/1‑150 (MQ=255)

                                                                                                                    tCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCAtt                                  >  2:1409901/1‑150 (MQ=255)

                                                                                                                    tCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCAtt                                  >  2:770925/1‑150 (MQ=255)

                                                                                                                          tACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGt                            <  2:354998/150‑1 (MQ=255)

                                                                                                                           aCTGCGAGCACTGAACTGCTGGCGGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTa                           <  2:273123/150‑1 (MQ=255)

                                                                                                                             tGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAAt                         <  2:584550/150‑1 (MQ=255)

                                                                                                                               cGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAg                       <  2:1553701/150‑1 (MQ=255)

                                                                                                                                gAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGc                      >  1:792693/1‑150 (MQ=255)

                                                                                                                                  gCACTGAACTGCGGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTTAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCaa                    <  2:535227/150‑1 (MQ=255)

                                                                                                                                     cTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGAGCAATGACTGACTTTCCATGCTCCCGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCGTAGTAATAGCAACCt                 >  2:14181/1‑150 (MQ=255)

                                                                                                                                     cTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGAGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACt                 >  2:200388/1‑150 (MQ=255)

                                                                                                                                        aaCTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTAcc              <  1:1505606/150‑1 (MQ=255)

                                                                                                                                         aCTGCTGGCTGCGTCTGCCAGCTGTGCATTTTTCCCCACGCCATCGCCGACTGAACGATGGCCTAGCGGAGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTAccc             <  2:107308/150‑1 (MQ=255)

                                                                                                                                         aCTGCTGGCAGACGCAGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGCACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTAccc             >  1:423947/1‑150 (MQ=255)

                                                                                                                                         aCTGCTGGCAGACGCAGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGGTCCTGTTGGCTCATTCCTTGTAATAGCAAACTAccc             >  2:1638785/1‑150 (MQ=255)

                                                                                                                                         aCTGCTGGCAGACGCAGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTAccc             >  1:1674316/1‑150 (MQ=255)

                                                                                                                                          ctgctgGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCa            >  1:1410930/1‑150 (MQ=255)

                                                                                                                                          ctgctgGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCa            >  1:800200/1‑150 (MQ=255)

                                                                                                                                            gctggctgCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCaaa          >  2:398623/1‑150 (MQ=255)

                                                                                                                                            gctggctgCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCaaa          >  1:117945/1‑150 (MQ=255)

                                                                                                                                            gctggctgCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCaaa          <  2:1219217/150‑1 (MQ=255)

                                                                                                                                               ggctgCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAAcc       <  2:1294068/150‑1 (MQ=255)

                                                                                                                                               ggctgCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAAcc       <  1:1403799/150‑1 (MQ=255)

                                                                                                                                                gctgCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACca      <  2:917859/150‑1 (MQ=255)

                                                                                                                                                   gCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACcagca   <  1:1570217/150‑1 (MQ=255)

                                                                                                                                                   gCGTCGGCCAGCTGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTATCCATGCTCACGAGATAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACcagca   <  1:319689/150‑1 (MQ=255)

                                                                                                                                                    cGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAATGGTGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACcagcag  >  2:432488/1‑150 (MQ=255)

                                                                                                                                                    cGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAATGGTGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACcagcag  <  1:432488/150‑1 (MQ=255)

                                                                                                                                                    cGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAATCTACCCAAAACcagcag  <  2:1700194/150‑1 (MQ=255)

AAACAATGTTTCCATCAATGACTTCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACCAGCAG  >  NC_000913/2962814‑2963111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`