Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,962,966 T→A 6.8% Q64L (CAG→CTG)  ygdB ← DUF2509 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,962,9660TA 6.8% 296.9 / 8.7 103Q64L (CAG→CTG) ygdBDUF2509 family protein
Reads supporting (aligned to +/- strand):  ref base T (44/51);  new base A (4/3);  total (48/55)
Fisher's exact test for biased strand distribution p-value = 7.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.61e-01

Only 100 of 105 total aligned reads displayed.

CAATGTTTCCATCAATGACTTCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACCAGCAG  >  NC_000913/2962817‑2963111
                                                                                                                                                     |                                                                                                                                                 
cAATGTTTCCATCAATGACTTCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCt                                                                                                                                                   >  1:1553701/1‑150 (MQ=255)
cAATGTTTCCATCAATGACTTCGCCTGTTCGCCACAACGAAAAGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCt                                                                                                                                                   <  1:1514277/150‑1 (MQ=255)
      ttCCATCAATGACTTCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCcagc                                                                                                                                             >  1:915292/1‑150 (MQ=255)
        ccATCAATGACTTCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGTTGGCTGCGTCTGCcagcag                                                                                                                                           <  2:546295/150‑1 (MQ=255)
        ccATCAATGACTTCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCcagcag                                                                                                                                           >  1:874308/1‑150 (MQ=255)
        ccATCAATGACTTCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCcagcag                                                                                                                                           >  1:996811/1‑150 (MQ=255)
        ccATCAATGACTTCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCAGACGCAGCcagcag                                                                                                                                           <  1:352038/150‑1 (MQ=255)
         cctCAAAGACTTCGCCGGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCAGACGCAGCCAGCAGt                                                                                                                                          <  2:1117549/148‑1 (MQ=255)
         cATCAATGACTTCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCAGACGCAGCCAGCAGt                                                                                                                                          <  2:910489/150‑1 (MQ=255)
             aaTGACTTCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCAt                                                                                                                                      >  1:1294068/1‑150 (MQ=255)
             aaTGACTTCGCCTGTTCGCCACAACGAAACGCCTCCATAACCGGCAATCAATAAGGCTTCATTATCTGACAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCTAACTGAGAGCACTGAACTCCTGGCAGCGTCTGCCAGCAGTGCaa                                                                                                                                      >  2:1664002/1‑149 (MQ=255)
                gACTTCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCAtttt                                                                                                                                   <  2:1298472/150‑1 (MQ=255)
                 aCTTCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCAttttt                                                                                                                                  >  1:107308/1‑150 (MQ=255)
                   ttCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTcc                                                                                                                                <  2:1006146/150‑1 (MQ=255)
                   ttCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAAACTGGGCATCGGTTTCAGAGTACTGCCAGCTCTGAACTGCTGGCTGCGTCTGCCAGCATTGCATTTTTcc                                                                                                                                >  2:355380/1‑150 (MQ=255)
                    tCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGGAATCAATAAGGCTTCATTATCTGCCTGTAATCGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTccc                                                                                                                               <  2:1543138/150‑1 (MQ=255)
                    tCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTccc                                                                                                                               <  2:964172/150‑1 (MQ=255)
                    tCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTccc                                                                                                                               <  2:706553/150‑1 (MQ=255)
                     cGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTcccc                                                                                                                              <  2:1295558/150‑1 (MQ=255)
                     cGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTAACTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTcccc                                                                                                                              <  2:253905/150‑1 (MQ=255)
                       ccTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCAc                                                                                                                            >  1:661390/1‑150 (MQ=255)
                         tGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGACTGCCAGCAGTGCATTTTTACCCACGc                                                                                                                          >  2:483457/1‑150 (MQ=255)
                            tCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAg                                                                                                                       <  1:885109/150‑1 (MQ=255)
                             cGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAgc                                                                                                                      >  2:398421/1‑150 (MQ=255)
                             cGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCGGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAgc                                                                                                                      <  2:73579/150‑1 (MQ=255)
                               ccACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTACACGCACACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGACTGCAAGCAGTGCATTTTTCCCCACGCCAgcgc                                                                                                                    >  1:256285/1‑150 (MQ=255)
                               ccACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAgcgc                                                                                                                    >  1:283440/1‑150 (MQ=255)
                                 acaACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCg                                                                                                                  <  1:226375/150‑1 (MQ=255)
                                   aaCGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGCTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGAc                                                                                                                <  2:584556/150‑1 (MQ=255)
                                        aaCGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAAc                                                                                                           <  2:915292/150‑1 (MQ=255)
                                          cGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGa                                                                                                         >  1:1145308/1‑150 (MQ=255)
                                          cGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCA‑TTTTCCCCCCGCCAGCGCCGACTGAACGAt                                                                                                        >  1:1053813/1‑150 (MQ=255)
                                            ccTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATg                                                                                                       <  2:576467/150‑1 (MQ=255)
                                             cTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGTCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATgg                                                                                                      <  1:1483512/150‑1 (MQ=255)
                                               tCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGcc                                                                                                    <  1:797468/150‑1 (MQ=255)
                                                cATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCt                                                                                                   >  2:1482881/1‑150 (MQ=255)
                                                   aaCCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTggc                                                                                                >  1:561228/1‑150 (MQ=255)
                                                     ccGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTggcgg                                                                                              >  1:152890/1‑150 (MQ=255)
                                                      cGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTggcggc                                                                                             >  1:1450620/1‑150 (MQ=255)
                                                       ggCAAGCAATCAGGCAGCACTCGCTCCCACTAAACGCCAACAAACCTGCGCAGCGGTTTCAGCGTACTGCGAGCACGGAACTGCTGGGGGCGTGTGCCAGCAGTGCATTTTTCCACACGCCAGCGCCGACTGAACGATGGCCTGgcggcg                                                                                            <  2:308271/150‑1 (MQ=255)
                                                         caatcaatAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCa                                                                                          <  2:189290/150‑1 (MQ=255)
                                                          aatcaatAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCaa                                                                                         >  2:1505606/1‑150 (MQ=255)
                                                           atcaatAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAAt                                                                                        >  1:1244569/1‑150 (MQ=255)
                                                              aatAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAAtgac                                                                                     >  1:459852/1‑150 (MQ=255)
                                                               atAATGCTTCATTCACTGCCAGTACACGCAAACTAACCTGGGCTTCGGTTGCAGCGTACTGCGAGCACGGAACTGCTGGCTGCGGCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAAtgact                                                                                    <  1:381333/150‑1 (MQ=255)
                                                                tAAGGCTTCATTATCTGCCAGTAACCGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAAtgactg                                                                                   <  1:1622351/150‑1 (MQ=255)
                                                                  aGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAAtgactgac                                                                                 >  1:930035/1‑150 (MQ=255)
                                                                  aGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAAtgactgac                                                                                 >  2:1199177/1‑150 (MQ=255)
                                                                  aGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGGTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAAtgactgac                                                                                 <  2:1402154/150‑1 (MQ=255)
                                                                   ggCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATg                                                                                                       <  1:1461011/127‑1 (MQ=255)
                                                                   ggCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATg                                                                                                       >  2:1461011/1‑127 (MQ=255)
                                                                          ttATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATg                                                                         <  1:1343419/150‑1 (MQ=255)
                                                                               tGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGCACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCAc                                                                    >  1:502303/1‑150 (MQ=255)
                                                                                gCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCAcg                                                                   <  1:182988/150‑1 (MQ=255)
                                                                                  cAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCAcgcg                                                                 <  1:1482881/150‑1 (MQ=255)
                                                                                       aaCGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAgg                                                            <  2:120244/150‑1 (MQ=255)
                                                                                        aCGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTT‑CATGCTCACGCGAGAGGCa                                                          <  2:1450620/150‑1 (MQ=255)
                                                                                         cGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCa                                                          <  2:283440/150‑1 (MQ=255)
                                                                                          gCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCaa                                                         >  2:1626731/1‑150 (MQ=255)
                                                                                           caaacaaaCCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCAt                                                                          >  1:985883/1‑132 (MQ=255)
                                                                                             aacaaacCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAAc                                                      <  2:1145308/150‑1 (MQ=255)
                                                                                                aaacCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCGGCGGCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGc                                                   <  2:1445679/150‑1 (MQ=255)
                                                                                                   ccTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGAt                                                <  2:843688/150‑1 (MQ=255)
                                                                                                     tGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATcc                                              <  1:398421/150‑1 (MQ=255)
                                                                                                       ggCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTg                                            >  1:570983/1‑150 (MQ=255)
                                                                                                         cATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGtt                                          <  2:1278748/150‑1 (MQ=255)
                                                                                                          aTCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTg                                         <  2:661390/150‑1 (MQ=255)
                                                                                                          aTCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTg                                         <  2:152890/150‑1 (MQ=255)
                                                                                                            cGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGc                                       <  2:429044/150‑1 (MQ=255)
                                                                                                               tttCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCa                                    >  1:1700194/1‑150 (MQ=255)
                                                                                                                 tCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCAtt                                  >  2:1409901/1‑150 (MQ=255)
                                                                                                                 tCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCAtt                                  >  1:354998/1‑150 (MQ=255)
                                                                                                                 tCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCAtt                                  >  2:770925/1‑150 (MQ=255)
                                                                                                                       aaCTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGt                            >  1:917859/2‑150 (MQ=255)
                                                                                                                        aCTGCGAGCACTGAACTGCTGGCGGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTa                           <  2:273123/150‑1 (MQ=255)
                                                                                                                          tGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAAt                         <  2:584550/150‑1 (MQ=255)
                                                                                                                            cGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAg                       <  2:1553701/150‑1 (MQ=255)
                                                                                                                             gAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGc                      >  1:792693/1‑150 (MQ=255)
                                                                                                                               gCACTGAACTGCGGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTTAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCaa                    <  2:535227/150‑1 (MQ=255)
                                                                                                                                  cTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGAGCAATGACTGACTTTCCATGCTCCCGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCGTAGTAATAGCAACCt                 >  2:14181/1‑150 (MQ=255)
                                                                                                                                  cTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGAGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACt                 >  2:200388/1‑150 (MQ=255)
                                                                                                                                     caCTGCTGGCAGACGCAGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTAcc              >  2:1328959/2‑150 (MQ=255)
                                                                                                                                     aaCTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTAcc              <  1:1505606/150‑1 (MQ=255)
                                                                                                                                      aCTGCTGGCTGCGTCTGCCAGCTGTGCATTTTTCCCCACGCCATCGCCGACTGAACGATGGCCTAGCGGAGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTAccc             <  2:107308/150‑1 (MQ=255)
                                                                                                                                      aCTGCTGGCAGACGCAGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGCACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTAccc             >  1:423947/1‑150 (MQ=255)
                                                                                                                                      aCTGCTGGCAGACGCAGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGGTCCTGTTGGCTCATTCCTTGTAATAGCAAACTAccc             >  2:1638785/1‑150 (MQ=255)
                                                                                                                                      aCTGCTGGCAGACGCAGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTAccc             >  1:1674316/1‑150 (MQ=255)
                                                                                                                                       ctgctgGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCa            >  1:1410930/1‑150 (MQ=255)
                                                                                                                                       ctgctgGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCa            >  1:800200/1‑150 (MQ=255)
                                                                                                                                         gctggctgCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCaaa          >  2:398623/1‑150 (MQ=255)
                                                                                                                                         gctggctgCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCaaa          >  1:117945/1‑150 (MQ=255)
                                                                                                                                         gctggctgCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCaaa          <  2:1219217/150‑1 (MQ=255)
                                                                                                                                            ggctgCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAAcc       <  2:1294068/150‑1 (MQ=255)
                                                                                                                                            ggctgCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAAcc       <  1:1403799/150‑1 (MQ=255)
                                                                                                                                             gctgCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACca      <  2:917859/150‑1 (MQ=255)
                                                                                                                                                gCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACcagca   <  1:1570217/150‑1 (MQ=255)
                                                                                                                                                gCGTCGGCCAGCTGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTATCCATGCTCACGAGATAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACcagca   <  1:319689/150‑1 (MQ=255)
                                                                                                                                                 cGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAATGGTGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACcagcag  >  2:432488/1‑150 (MQ=255)
                                                                                                                                                 cGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAATGGTGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACcagcag  <  1:432488/150‑1 (MQ=255)
                                                                                                                                                 cGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAATCTACCCAAAACcagcag  <  2:1700194/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
CAATGTTTCCATCAATGACTTCGCCTGTTCGCCACAACGAAACGCCTTCATAACCGGCAATCAATAAGGCTTCATTATCTGCCAGTAAACGCAAACAAACCTGGGCATCGGTTTCAGCGTACTGCGAGCACTGAACTGCTGGCTGCGTCTGCCAGCAGTGCATTTTTCCCCACGCCAGCGCCGACTGAACGATGGCCTGGCGGCGCAATGACTGACTTTCCATGCTCACGCGAGAGGCAAAACTGCGATCCTGTTGGCTCATTCCTTGTAATAGCAAACTACCCAAAACCAGCAG  >  NC_000913/2962817‑2963111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑