Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,976,320 T→G 5.4% noncoding (66/82 nt) omrB ← sRNA antisense regulator downregulates OM proteins and curli; positively regulated by OmpR/EnvZ, Hfq‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,976,3200TG 5.4% 360.5 / 8.1 125noncoding (66/82 nt)omrBsRNA antisense regulator downregulates OM proteins and curli; positively regulated by OmpR/EnvZ, Hfq‑dependent
Reads supporting (aligned to +/- strand):  ref base T (57/61);  new base G (3/4);  total (60/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.35e-01

Only 100 of 127 total aligned reads displayed.

ACCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGCAA  >  NC_000913/2976171‑2976469
                                                                                                                                                      |                                                                                                                                                     
  caatcaatACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCt                                                                                                                                                     >  2:327931/1‑150 (MQ=255)
  caatcaatACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCt                                                                                                                                                     <  2:1373288/150‑1 (MQ=255)
   aatcaatACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTg                                                                                                                                                    >  1:810208/1‑150 (MQ=255)
   aatcaatACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTg                                                                                                                                                    >  1:1489143/1‑150 (MQ=255)
     tcaatACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTgcg                                                                                                                                                  <  1:166533/150‑1 (MQ=255)
     tcaatACCTCTGGGATCTTGAGTGTGGTCTGCACGACGCCTCTTCGCCAGCGAGAAAAAGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTgag                                                                                                                                                  >  2:86802/1‑148 (MQ=255)
       aatACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCCGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTAGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCa                                                                                                                                                >  1:1315083/1‑150 (MQ=255)
       aatACCTCTGGGATCTACAATTTAGAAACCACAACGAAACATCGACAGCGAGAAAAAGAAAAGGAATTAATGTAAATGAAACGAGGTGTGTAAAATGTATTATAATTTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCa                                                                                                                                                >  2:224388/1‑150 (MQ=255)
           ccTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCt                                                                                                                                            >  1:100615/1‑150 (MQ=255)
            ctctGGGATCTTGATCGGGGGCTCCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGGGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTg                                                                                                                                           <  1:818363/150‑1 (MQ=255)
             tctGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTgg                                                                                                                                          >  2:651598/1‑150 (MQ=255)
             tctGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTgg                                                                                                                                          >  2:1565260/1‑150 (MQ=255)
             tctGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCAGATGCGCAggtttt                                                                                                                                          <  1:1577087/150‑5 (MQ=255)
               tGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCGACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGgtg                                                                                                                                        >  2:302405/1‑150 (MQ=255)
                gggATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGgtgt                                                                                                                                       <  1:905299/150‑1 (MQ=255)
                gggATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGgtgt                                                                                                                                       <  1:899187/150‑1 (MQ=255)
                gggATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCAGATGCGCAggttttttt                                                                                                                                       <  1:1321665/150‑8 (MQ=255)
                    tCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAAtt                                                                                                                                   <  1:614152/150‑1 (MQ=255)
                     cTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTc                                                                                                                                  <  2:1642504/150‑1 (MQ=255)
                        gATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCAtg                                                                                                                               <  1:358088/150‑1 (MQ=255)
                          ttGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCAtgtg                                                                                                                             <  1:990642/150‑1 (MQ=255)
                          ttGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCAtgtg                                                                                                                             <  2:507156/150‑1 (MQ=255)
                           tgtgGTATGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACTAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCAtgtg                                                                                                                             <  1:1615036/150‑1 (MQ=255)
                             tgGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCAtgccgac                                                                                                                          >  1:503891/1‑145 (MQ=255)
                               gTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATATGCGCAGGATGGTGTAATTCATGTGCTCaa                                                                                                                        >  1:328654/1‑150 (MQ=255)
                                tCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAAc                                                                                                                       >  1:1306185/1‑150 (MQ=255)
                                 cTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAAcc                                                                                                                      <  1:553272/150‑1 (MQ=255)
                                  tGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAAccc                                                                                                                     >  1:1527215/1‑150 (MQ=255)
                                   gCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCg                                                                                                                    >  1:1335177/1‑150 (MQ=255)
                                    cACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGa                                                                                                                   >  2:1118140/1‑150 (MQ=255)
                                           acTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTATTGTTACAGATTGATGAGCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGAc                                                                                                            <  2:1110517/150‑1 (MQ=255)
                                           acTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGAc                                                                                                            <  2:807865/150‑1 (MQ=255)
                                              ctTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACt                                                                                                           <  2:1683064/148‑1 (MQ=255)
                                              ctTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACt                                                                                                           >  1:1683064/1‑148 (MQ=255)
                                               ttCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCa                                                                                                        >  2:193612/1‑150 (MQ=255)
                                                tCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCAc                                                                                                       >  1:512080/1‑150 (MQ=255)
                                                 ggCCAGCGAGAAAACGGAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCAcc                                                                                                      <  2:379525/149‑1 (MQ=255)
                                                  gCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCt                                                                                                     >  2:6186/1‑150 (MQ=255)
                                                      gCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCa                                                                                                 <  1:327931/150‑1 (MQ=255)
                                                      gCGAGAAAACGAAAAGGAATGAAGGGAAATGCAACGATGTGTGTAAATTGTCGGTTACTGTTACAGATTTATGATCTGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCa                                                                                                 <  2:126657/150‑1 (MQ=255)
                                                        gagaAAACGCAAAGTAATTAAGGTAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACcaatcaat                                                                                               <  1:1048020/150‑1 (MQ=255)
                                                        gagaAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAAt                                                                                               <  2:1242012/150‑1 (MQ=255)
                                                           aaaaCGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATAcc                                                                                            <  2:156172/150‑1 (MQ=255)
                                                           aaaaCGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATAcc                                                                                            <  2:1489143/150‑1 (MQ=255)
                                                               cGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGTGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTg                                                                                        >  1:1004158/1‑150 (MQ=255)
                                                               cGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTg                                                                                        <  2:810208/150‑1 (MQ=255)
                                                                 cAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATTACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTggg                                                                                      <  2:215520/150‑1 (MQ=255)
                                                                   aaGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGAt                                                                                    >  2:265747/1‑150 (MQ=255)
                                                                     ggAATGAAGGGCAATGCAACTAGGTGTGTATATTGTCTGTTACTGTTACAGATTGTTGACCGGCAAAAAAAACCTGCGCATCTGCGGTGGCTGGTGTAATTCATGTGCTCAACCAGAATTTGACTTCACCTATCAATACCTCTGGGATca                                                                                  <  1:407651/150‑1 (MQ=255)
                                                                     ggAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTCATCACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTCACTTCACCTAGCCATACCTCGGGGCTca                                                                                  >  2:1404284/1‑150 (MQ=255)
                                                                             gggAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTa                                                                          >  1:625942/1‑150 (MQ=255)
                                                                              ggAAATGCAACGAGGTGTTTAAATTGTCGGTTACTGTTACAGATTGATGACCTGAAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCTTGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAg                                                                         <  1:1626695/150‑1 (MQ=255)
                                                                                aaaTGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCa                                                                       <  1:6186/150‑1 (MQ=255)
                                                                                 aaTGCAACGAGGGGTGTAAATTGTCGGTGACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCaa                                                                      <  1:1550746/150‑1 (MQ=255)
                                                                                  atccAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCAGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAAc                                                                     <  1:1194166/147‑1 (MQ=255)
                                                                                  aTGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAAc                                                                     >  1:494039/1‑150 (MQ=255)
                                                                                  aTGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAAc                                                                     <  1:1620021/150‑1 (MQ=255)
                                                                                   tGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGAATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACATCTGGGCTCACCACTTTAGCAAcc                                                                    >  1:256045/1‑150 (MQ=255)
                                                                                   tGCAACGAGGTCTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTTCGCATCTGCTCAGGCTGGAGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAAcc                                                                    <  1:224388/150‑1 (MQ=255)
                                                                                     cAACGAGGTGTGTAATTTGTCGGTTACTGTTACAGATTGATGTCCGGCAAAAAAATCCTGCGCATCTGCGCAGGCTTGTGTAATTCATGTGCTCTACCCTAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTg                                                                  <  1:1211204/150‑1 (MQ=255)
                                                                                        cGAGGTGTGTATATTGTCGGTTACTGTTTCAGTTTGATGACCTGCATTTAAACCCTGCGCATCTGCGGAGGCTGGTGTAAGTGTTGTTCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAg                                                               <  1:705980/150‑1 (MQ=255)
                                                                                        cGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAg                                                               <  2:1527215/150‑1 (MQ=255)
                                                                                            gtgtgtAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCaa                                                           <  1:1118140/150‑1 (MQ=255)
                                                                                             tgtttATATTGTCTGTTACTGTTACAGATTGATGACCTCAAAAAAAATCCTGCGCATATGCGCAGGCTGGTGTAATTCATGTTCTCAACACGAACTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCaaa                                                          <  1:28985/150‑1 (MQ=255)
                                                                                             tgtgtAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCaaa                                                          <  2:512080/150‑1 (MQ=255)
                                                                                              gtgtAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAAc                                                         >  1:194465/1‑150 (MQ=255)
                                                                                                 tAAATTGTCGGTTACTGTTACAGATTGATGCCCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTATAGCAACCTGACGCCAAACgac                                                      >  1:201277/1‑148 (MQ=255)
                                                                                                       gTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGc                                                >  2:1448761/1‑150 (MQ=255)
                                                                                                          ggTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGCAGTTGACTTCCCCTATCAATACCTCTGGGATCCCCACTTTAGCTACCTCAAGCCAACCGCCAACTGCGGt                                             >  2:314322/1‑150 (MQ=255)
                                                                                                          ggTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCCCCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGt                                             >  1:1288313/1‑150 (MQ=255)
                                                                                                          ggTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGt                                             >  1:1281617/1‑150 (MQ=255)
                                                                                                          ggTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTAATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCAACACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGt                                             >  1:1248027/1‑150 (MQ=255)
                                                                                                           gTTACTGTTACAGATTGATGACTGGCAAAAAAATCCTTCGCAGATGCTCAGGCTTGTGTAATTCATGTGCTCTACCCGATTTTTATTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTc                                            <  1:1054720/150‑1 (MQ=255)
                                                                                                            ttACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCa                                           >  1:1457764/1‑150 (MQ=255)
                                                                                                               cTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATc                                        >  2:1501543/1‑150 (MQ=255)
                                                                                                               atGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTTGTGTAATTCATGTGCTCAACCCAAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATc                                        <  2:379269/149‑1 (MQ=255)
                                                                                                                tGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCg                                       <  1:802745/150‑1 (MQ=255)
                                                                                                                tGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCg                                       <  2:1350082/150‑1 (MQ=255)
                                                                                                                 gTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCCCCAGCGGTCAATCGc                                      >  2:45984/1‑150 (MQ=255)
                                                                                                                   tACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCaa                                    >  2:520515/1‑150 (MQ=255)
                                                                                                                    aCAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAAc                                   >  1:1138674/1‑150 (MQ=255)
                                                                                                                       gATTGATGCCCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGc                                >  2:1206452/1‑150 (MQ=255)
                                                                                                                       gATTGATGACCGGCAAAAAAAACCTGCGCATCTGTGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGc                                <  2:1004158/150‑1 (MQ=255)
                                                                                                                           gatgaCCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGttt                            >  2:1638144/1‑150 (MQ=255)
                                                                                                                             tgaCCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCg                          <  1:265747/150‑1 (MQ=255)
                                                                                                                             tgaCCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGGCAATCGCAACAGCGTTTCg                          <  2:90420/150‑1 (MQ=255)
                                                                                                                             ggagcGGCAAATATAACCTGCGAATCTCCGCAGGCTGTTGTAATTGATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCg                          <  2:1058718/146‑1 (MQ=255)
                                                                                                                              gaCCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGc                         <  2:1306185/150‑1 (MQ=255)
                                                                                                                               aCCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCa                        >  2:1346314/1‑150 (MQ=255)
                                                                                                                                       aaaaaaaCCTGCGCATCTGCGCAGGCTGGTGTAAGTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTa                 <  1:1713491/150‑1 (MQ=255)
                                                                                                                                         aaaaaCCTGCGCATCTGCTCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCTCTTTAGCAAGCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAAc               <  1:302405/150‑1 (MQ=255)
                                                                                                                                          ccagccTGCGCAGATGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAAcc              >  2:451876/5‑150 (MQ=255)
                                                                                                                                          ccagccTGCGCAGATGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGACACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAAcc              >  1:207038/5‑150 (MQ=255)
                                                                                                                                            agccTGCGCAGATGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTAAATCGCAACAGCGTTTCGAAAAGTGTAACCaa            >  2:533872/3‑150 (MQ=255)
                                                                                                                                            aaCCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCaa            >  2:908825/1‑150 (MQ=255)
                                                                                                                                              ccTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAAAACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAg          >  1:894002/1‑150 (MQ=255)
                                                                                                                                                tGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCCCAAAGTGTAACCAAAGGt        >  2:433813/1‑150 (MQ=255)
                                                                                                                                                 gcgcATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGtt       >  1:917554/1‑150 (MQ=255)
                                                                                                                                                 gcgcATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGtt       <  1:86802/150‑1 (MQ=255)
                                                                                                                                                      tCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGCaa  >  2:1682798/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
ACCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGCAA  >  NC_000913/2976171‑2976469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: