Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,976,321 C→A 7.2% noncoding (65/82 nt) omrB ← sRNA antisense regulator downregulates OM proteins and curli; positively regulated by OmpR/EnvZ, Hfq‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,976,3210CA 7.2% 369.5 / 10.6 125noncoding (65/82 nt)omrBsRNA antisense regulator downregulates OM proteins and curli; positively regulated by OmpR/EnvZ, Hfq‑dependent
Reads supporting (aligned to +/- strand):  ref base C (56/59);  new base A (4/6);  total (60/65)
Fisher's exact test for biased strand distribution p-value = 7.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 126 total aligned reads displayed.

CCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGCAA  >  NC_000913/2976172‑2976469
                                                                                                                                                      |                                                                                                                                                    
ccAATCAATACCTCTGGGATCTACAATTTAGTCTTCACGACGACTATTCGCCAGCAAGAAAACGAAAAGGAATGAAGTGAAATGCAACGAGGTATCTAAATTTTCTGTTACTGTTACAGATTGATGCCCGGCAAAAAAAACCTGCGCATc                                                                                                                                                      >  2:313239/1‑150 (MQ=255)
 caatcaatACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCt                                                                                                                                                     >  2:327931/1‑150 (MQ=255)
  aatcaatACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTg                                                                                                                                                    >  1:810208/1‑150 (MQ=255)
  aatcaatACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTg                                                                                                                                                    >  1:1489143/1‑150 (MQ=255)
    tcaatACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACTAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAAACCTGCGCATCTgc                                                                                                                                                   <  2:1326262/150‑1 (MQ=255)
    tcaatACCTCTGGGATCTTGAGTGTGGTCTGCACGACGCCTCTTCGCCAGCGAGAAAAAGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTgag                                                                                                                                                  >  2:86802/1‑148 (MQ=255)
      aatACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCCGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTAGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCa                                                                                                                                                >  1:1315083/1‑150 (MQ=255)
      aatACCTCTGGGATCTACAATTTAGAAACCACAACGAAACATCGACAGCGAGAAAAAGAAAAGGAATTAATGTAAATGAAACGAGGTGTGTAAAATGTATTATAATTTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCa                                                                                                                                                >  2:224388/1‑150 (MQ=255)
          ccTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCt                                                                                                                                            >  1:100615/1‑150 (MQ=255)
           ctctGGGATCTTGATCGGGGGCTCCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGGGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTg                                                                                                                                           <  1:818363/150‑1 (MQ=255)
            tctGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTgg                                                                                                                                          >  2:651598/1‑150 (MQ=255)
            tctGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTgg                                                                                                                                          >  2:1565260/1‑150 (MQ=255)
               gggATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGgtgt                                                                                                                                       <  1:905299/150‑1 (MQ=255)
               gggATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGgtgt                                                                                                                                       <  1:899187/150‑1 (MQ=255)
               gggATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCAGATGCGCAggttttttt                                                                                                                                       <  1:1321665/150‑8 (MQ=255)
                 gATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTaa                                                                                                                                     >  2:1211204/1‑150 (MQ=255)
                       gATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCAtg                                                                                                                               <  1:358088/150‑1 (MQ=255)
                         ttGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCAtgtg                                                                                                                             <  2:507156/150‑1 (MQ=255)
                         ttGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCAtgtg                                                                                                                             <  1:990642/150‑1 (MQ=255)
                          tgtgGTATGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACTAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCAtgtg                                                                                                                             <  1:1615036/150‑1 (MQ=255)
                           ttgGTCTGCACGTCGACTCTTAGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAATCCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCt                                                                                                                           <  1:237872/149‑1 (MQ=255)
                            tgGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCAtgccgac                                                                                                                          >  1:503891/1‑145 (MQ=255)
                             ggTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCa                                                                                                                         <  1:672300/150‑1 (MQ=255)
                              gTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATATGCGCAGGATGGTGTAATTCATGTGCTCaa                                                                                                                        >  1:328654/1‑150 (MQ=255)
                               tCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAAc                                                                                                                       >  1:1306185/1‑150 (MQ=255)
                                cTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAAcc                                                                                                                      <  1:553272/150‑1 (MQ=255)
                                 tGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAAccc                                                                                                                     >  1:1527215/1‑150 (MQ=255)
                                  gCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCg                                                                                                                    >  1:1335177/1‑150 (MQ=255)
                                   cACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGa                                                                                                                   >  2:1118140/1‑150 (MQ=255)
                                          acTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGAc                                                                                                            <  2:807865/150‑1 (MQ=255)
                                             ctTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACt                                                                                                           <  2:1683064/148‑1 (MQ=255)
                                             ctTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACt                                                                                                           >  1:1683064/1‑148 (MQ=255)
                                               tCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCAc                                                                                                       >  1:512080/1‑150 (MQ=255)
                                                 gCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCt                                                                                                     >  2:6186/1‑150 (MQ=255)
                                                   cAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTAt                                                                                                   >  1:1378674/1‑150 (MQ=255)
                                                     gCGAGAAAACGCAAAGTAATGAAGGGAAATTCAACGAGGTGTGTAAATTGTCGGTTACTTTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCa                                                                                                 <  1:476420/150‑1 (MQ=255)
                                                       gagaAAACGCAAAGTAATTAAGGTAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACcaatcaat                                                                                               <  1:1048020/150‑1 (MQ=255)
                                                       gagaAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAAt                                                                                               <  2:1242012/150‑1 (MQ=255)
                                                          aaaaCGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATAcc                                                                                            <  2:1489143/150‑1 (MQ=255)
                                                          aaaaCGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATAcc                                                                                            <  2:156172/150‑1 (MQ=255)
                                                              cGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGTGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTg                                                                                        >  1:1004158/1‑150 (MQ=255)
                                                              cGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTg                                                                                        <  2:810208/150‑1 (MQ=255)
                                                              cGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATAACTCTg                                                                                        >  1:90420/1‑150 (MQ=255)
                                                                cAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATTACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTggg                                                                                      <  2:215520/150‑1 (MQ=255)
                                                                  aaGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGAt                                                                                    >  2:265747/1‑150 (MQ=255)
                                                                    ggAATGAAGGGCAATGCAACTAGGTGTGTATATTGTCTGTTACTGTTACAGATTGTTGACCGGCAAAAAAAACCTGCGCATCTGCGGTGGCTGGTGTAATTCATGTGCTCAACCAGAATTTGACTTCACCTATCAATACCTCTGGGATca                                                                                  <  1:407651/150‑1 (MQ=255)
                                                                    ggAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTCATCACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTCACTTCACCTAGCCATACCTCGGGGCTca                                                                                  >  2:1404284/1‑150 (MQ=255)
                                                                     gAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATcac                                                                                 <  2:100615/150‑1 (MQ=255)
                                                                        tGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATcaccac                                                                              <  1:545769/150‑1 (MQ=255)
                                                                            gggAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTa                                                                          >  1:625942/1‑150 (MQ=255)
                                                                             ggAAATGCAACGAGGTGTTTAAATTGTCGGTTACTGTTACAGATTGATGACCTGAAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCTTGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAg                                                                         <  1:1626695/150‑1 (MQ=255)
                                                                             ggAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAg                                                                         <  1:313239/150‑1 (MQ=255)
                                                                               aaaTGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCa                                                                       <  1:6186/150‑1 (MQ=255)
                                                                                aaTGCAACGAGGGGTGTAAATTGTCGGTGACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCaa                                                                      <  1:1550746/150‑1 (MQ=255)
                                                                                 atccAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCAGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAAc                                                                     <  1:1194166/147‑1 (MQ=255)
                                                                                 aTGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAAc                                                                     <  1:1620021/150‑1 (MQ=255)
                                                                                  tGCAACGAGGTCTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTTCGCATCTGCTCAGGCTGGAGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAAcc                                                                    <  1:224388/150‑1 (MQ=255)
                                                                                   gCAACGAGTTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGTCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCt                                                                   <  2:1534154/150‑1 (MQ=255)
                                                                                    cAACGAGGTGTGTAATTTGTCGGTTACTGTTACAGATTGATGTCCGGCAAAAAAATCCTGCGCATCTGCGCAGGCTTGTGTAATTCATGTGCTCTACCCTAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTg                                                                  <  1:1211204/150‑1 (MQ=255)
                                                                                       cGAGGTGTGTATATTGTCGGTTACTGTTTCAGTTTGATGACCTGCATTTAAACCCTGCGCATCTGCGGAGGCTGGTGTAAGTGTTGTTCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAg                                                               <  1:705980/150‑1 (MQ=255)
                                                                                       cGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAg                                                               <  1:1565260/150‑1 (MQ=255)
                                                                                           gtgtgtAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCaa                                                           <  1:1118140/150‑1 (MQ=255)
                                                                                            tgtttATATTGTCTGTTACTGTTACAGATTGATGACCTCAAAAAAAATCCTGCGCATATGCGCAGGCTGGTGTAATTCATGTTCTCAACACGAACTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCaaa                                                          <  1:28985/150‑1 (MQ=255)
                                                                                            tgtgtAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCaaa                                                          <  2:512080/150‑1 (MQ=255)
                                                                                             gtgtAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAAc                                                         >  2:409842/1‑150 (MQ=255)
                                                                                                tAAATTGTCGGTTACTGTTACAGATTGATGCCCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTATAGCAACCTGACGCCAAACgac                                                      >  1:201277/1‑148 (MQ=255)
                                                                                                   aTTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGccacc                                                   >  1:180820/1‑150 (MQ=255)
                                                                                                      gTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGc                                                >  2:1448761/1‑150 (MQ=255)
                                                                                                         ggTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGCAGTTGACTTCCCCTATCAATACCTCTGGGATCCCCACTTTAGCTACCTCAAGCCAACCGCCAACTGCGGt                                             >  2:314322/1‑150 (MQ=255)
                                                                                                         ggTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCCCCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGt                                             >  1:1288313/1‑150 (MQ=255)
                                                                                                         ggTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGt                                             >  1:1281617/1‑150 (MQ=255)
                                                                                                         ggTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTAATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCAACACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGt                                             >  1:1248027/1‑150 (MQ=255)
                                                                                                          gTTACTGTTACAGATTGATGACTGGCAAAAAAATCCTTCGCAGATGCTCAGGCTTGTGTAATTCATGTGCTCTACCCGATTTTTATTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTc                                            <  1:1054720/150‑1 (MQ=255)
                                                                                                              cTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATc                                        >  2:1501543/1‑150 (MQ=255)
                                                                                                              atGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTTGTGTAATTCATGTGCTCAACCCAAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATc                                        <  2:379269/149‑1 (MQ=255)
                                                                                                               tGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCg                                       <  2:1350082/150‑1 (MQ=255)
                                                                                                                gTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGcca                                                     <  1:1654037/135‑1 (MQ=255)
                                                                                                                gTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGcca                                                     >  2:1654037/1‑135 (MQ=255)
                                                                                                                gTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCCCCAGCGGTCAATCGc                                      >  2:45984/1‑150 (MQ=255)
                                                                                                                  tACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCaa                                    >  2:520515/1‑150 (MQ=255)
                                                                                                                   aCAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAAc                                   >  1:1138674/1‑150 (MQ=255)
                                                                                                                      gATTGATGCCCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGc                                >  2:1206452/1‑150 (MQ=255)
                                                                                                                      gATTGATGACCGGCAAAAAAAACCTGCGCATCTGTGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGc                                <  2:1004158/150‑1 (MQ=255)
                                                                                                                          gatgaCCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGttt                            >  2:1638144/1‑150 (MQ=255)
                                                                                                                            tgaCCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCg                          <  2:194465/150‑1 (MQ=255)
                                                                                                                            tgaCCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCg                          <  1:265747/150‑1 (MQ=255)
                                                                                                                            tgaCCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGGCAATCGCAACAGCGTTTCg                          <  2:90420/150‑1 (MQ=255)
                                                                                                                            ggagcGGCAAATATAACCTGCGAATCTCCGCAGGCTGTTGTAATTGATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCg                          <  2:1058718/146‑1 (MQ=255)
                                                                                                                             gaCCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGc                         <  2:1306185/150‑1 (MQ=255)
                                                                                                                              aCCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCa                        >  2:1346314/1‑150 (MQ=255)
                                                                                                                                      aaaaaaaCCTGCGCATCTGCGCAGGCTGGTGTAAGTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTa                 <  1:1713491/150‑1 (MQ=255)
                                                                                                                                        aaaaaCCTGCGCATCTGCTCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCTCTTTAGCAAGCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAAc               <  1:302405/150‑1 (MQ=255)
                                                                                                                                         ccagccTGCGCAGATGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAAcc              >  2:451876/5‑150 (MQ=255)
                                                                                                                                         ccagccTGCGCAGATGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGACACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAAcc              >  1:207038/5‑150 (MQ=255)
                                                                                                                                           agccTGCGCAGATGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTAAATCGCAACAGCGTTTCGAAAAGTGTAACCaa            >  2:533872/3‑150 (MQ=255)
                                                                                                                                           aaCCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCaa            >  2:908825/1‑150 (MQ=255)
                                                                                                                                             ccTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAAAACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAg          >  1:894002/1‑150 (MQ=255)
                                                                                                                                               tGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCCCAAAGTGTAACCAAAGGt        >  2:433813/1‑150 (MQ=255)
                                                                                                                                                   cATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGc    >  1:839837/1‑150 (MQ=255)
                                                                                                                                                     tCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGCaa  >  2:1682798/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                    
CCAATCAATACCTCTGGGATCTTGATTGTGGTCTGCACGACGACTCTTCGCCAGCGAGAAAACGCAAAGGAATGAAGGGAAATGCAACGAGGTGTGTAAATTGTCGGTTACTGTTACAGATTGATGACCGGCAAAAAAAACCTGCGCATCTGCGCAGGCTGGTGTAATTCATGTGCTCAACCCGAAGTTGACTTCACCTATCAATACCTCTGGGATCACCACTTTAGCAACCTGAAGCCAAACGCCACCAGCGGTCAATCGCAACAGCGTTTCGCAAAGTGTAACCAAAGGTTTGCAA  >  NC_000913/2976172‑2976469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: