BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	297,213	G→C	5.2%	pseudogene (8/49 nt)	ptwF →	tRNA‑OTHER

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	297,213	0	G	C	5.2%	422.0 / 11.7	136	pseudogene (8/49 nt)	ptwF	tRNA‑OTHER
Reads supporting (aligned to +/- strand): ref base G (71/57); new base C (4/3); total (75/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.88e-02

Only 100 of 137 total aligned reads displayed.

TGCTGGTGTAAGTAAATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATACTGTTCTGACATCAGG  >  NC_000913/297065‑297362

tgctgGTGTAAGTAAATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGt                                                                                                                                                      >  1:555989/1‑150 (MQ=255)

tgctgGTGTAAGTAAATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATAAAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAAAGCATTAACATCGGATTCGt                                                                                                                                                      >  1:78456/1‑150 (MQ=255)

  ctgGTGTAAGTAAATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTaa                                                                                                                                                    <  1:163182/150‑1 (MQ=255)

    ggTGTAAGTAAATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATg                                                                                                                                                  >  1:869630/1‑150 (MQ=255)

      tgtAAGTAAATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCg                                                                                                                                                >  1:495620/1‑150 (MQ=255)

        taagtaaATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGaa                                                                                                                                              >  2:1430101/1‑150 (MQ=255)

        taagtaaATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTACGAATGCGat                                                                                                                                              <  1:653379/150‑2 (MQ=255)

         aagtaaATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTACGAATGCGatg                                                                                                                                             <  1:1156357/150‑3 (MQ=255)

          agtaaATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAgg                                                                                                                                            >  1:1024459/1‑150 (MQ=255)

               aTGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTa                                                                                                                                       <  1:1531004/150‑1 (MQ=255)

                   aGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGtt                                                                                                                                   <  1:1114012/150‑1 (MQ=255)

                   aGGGAATAAAAATCAACTTTTGTTAATTTCAATCAACGGGTTTTTTGCTATAACTGGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGtt                                                                                                                                   <  1:840779/150‑1 (MQ=255)

                      gattAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGa                                                                                                                                <  2:330896/147‑1 (MQ=255)

                      gAATAACCATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGa                                                                                                                                >  1:1579871/1‑150 (MQ=255)

                      gAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGa                                                                                                                                >  1:1586290/1‑150 (MQ=255)

                      gAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGa                                                                                                                                >  2:372711/1‑150 (MQ=255)

                             cATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCtat                                                                                                                         >  2:474532/1‑150 (MQ=255)

                              aTCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCtatt                                                                                                                        >  2:480481/1‑150 (MQ=255)

                                     tttGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCa                                                                                                                 <  1:1692259/150‑1 (MQ=255)

                                       tGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGTGAAGGTCGTAGGTTCGACTCCTATTATCGGCAcc                                                                                                               <  1:1571368/150‑1 (MQ=255)

                                       tGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCAcc                                                                                                               <  1:751264/150‑1 (MQ=255)

                                       tGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCAcc                                                                                                               <  1:1430101/150‑1 (MQ=255)

                                        gTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATTTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCTCATTCGTAATGCGAATGTCGTAGGTTCGACTCCTATTATCGGCACCa                                                                                                              >  1:1040327/1‑150 (MQ=255)

                                        gTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTTGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCa                                                                                                              >  2:1479019/1‑150 (MQ=255)

                                            aTTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTaa                                                                                                          >  2:636860/1‑150 (MQ=255)

                                              ttCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTaaaa                                                                                                        >  2:1366460/1‑150 (MQ=255)

                                                 aatcaaCGGGTTTATTGCTATAACTTGGTTTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCa                                                                                                     <  1:1623311/150‑1 (MQ=255)

                                                 aatcaaCGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCa                                                                                                     <  1:474532/150‑1 (MQ=255)

                                                    caaCGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAag                                                                                                  <  2:131851/150‑1 (MQ=255)

                                                       cGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGt                                                                                               >  2:446131/1‑150 (MQ=255)

                                                         ggTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATTAATAAACATCTCATTCGTAATGCGAAGGTCGTAGGTTCGACTCATATTATCGGCACCATTAAAATCAAAAAGTTa                                                                                             >  1:1470322/1‑150 (MQ=37)

                                                         ggTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTa                                                                                             >  2:1065123/1‑150 (MQ=255)

                                                            ttATTGCTATAACTTGGTTTTATATAATATAAATTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTACGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTAccc                                                                                          <  2:1066750/150‑1 (MQ=255)

                                                            ttATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAGGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTAccc                                                                                          <  1:1605826/150‑1 (MQ=255)

                                                                   tataACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATAttt                                                                                   >  1:575967/1‑150 (MQ=255)

                                                                   tataACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATAttt                                                                                   >  2:1165747/1‑150 (MQ=255)

                                                                    ataACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTa                                                                                  >  2:1294051/1‑150 (MQ=255)

                                                                     taACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGAGAAGGTCGTAGGTTCGAATCATATTATCTGCACCATTAAAATCAAAGAGTTAACCCATATTTaa                                                                                 >  1:286636/1‑150 (MQ=255)

                                                                      aaCTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTaaa                                                                                >  1:726095/1‑150 (MQ=255)

                                                                      aaCTTGGGGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAACTGCATAAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTaaa                                                                                <  2:885815/150‑1 (MQ=255)

                                                                         ttGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATac                                                                             >  1:396436/1‑150 (MQ=255)

                                                                          tGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTAccc                                                                                          <  2:1678635/136‑1 (MQ=255)

                                                                          tGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTA‑TATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATacac                                                                           <  2:1579871/150‑1 (MQ=255)

                                                                            gtgtTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATAcacc                                                                          >  2:882397/1‑150 (MQ=255)

                                                                             tgtTTTATAATATAAAGTATGTTGTTTTGATTGATTTCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGTTCGTAGGTTAGACTAATATTATCTGAACAATTAAAATAAAAGATTTACACAAAATTTAAAAacacaa                                                                         >  1:983837/1‑148 (MQ=255)

                                                                              gtTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATAcaccac                                                                        <  2:13117/150‑1 (MQ=255)

                                                                              gtTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCAAATTTAAATAcaccac                                                                        >  2:254838/1‑150 (MQ=255)

                                                                               ttttATAATATAAAGTATGTTGTTTTTATTGATTTCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACg                                                                       <  2:1486971/150‑1 (MQ=255)

                                                                                   ataatataaAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTtc                                                                   <  1:1158925/150‑1 (MQ=255)

                                                                                    taatataaAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTtcc                                                                  >  2:1021227/1‑150 (MQ=255)

                                                                                    taatataaAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTtcc                                                                  >  1:1699127/1‑150 (MQ=255)

                                                                                     aatataaAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCata                                                                                      >  2:74230/1‑129 (MQ=255)

                                                                                     aatataaAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCata                                                                                      <  1:74230/129‑1 (MQ=255)

                                                                                       tataaAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTtcctcc                                                               <  1:415663/150‑1 (MQ=255)

                                                                                        ataaAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTcctcct                                                              >  1:199902/1‑150 (MQ=255)

                                                                                         taaAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACCCCACGTTTCCTCCtg                                                             >  1:1491376/1‑150 (MQ=255)

                                                                                         taaAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCtg                                                             >  2:813127/1‑150 (MQ=255)

                                                                                         taaAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCAATATTTAAATACACCACGTTTCCTCCtg                                                             >  1:824271/1‑150 (MQ=255)

                                                                                           aaGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCtgtg                                                           >  2:843552/1‑150 (MQ=255)

                                                                                              tATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCg                                                        <  1:882397/150‑1 (MQ=255)

                                                                                              tATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCg                                                        <  2:457079/150‑1 (MQ=255)

                                                                                              tATGTTGTTGTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCTTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCg                                                        <  1:118823/150‑1 (MQ=255)

                                                                                               aTGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGt                                                       >  2:33960/1‑150 (MQ=255)

                                                                                                tgttgtTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTtcct                                                                 <  2:860878/139‑1 (MQ=255)

                                                                                                    gttTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACACCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTtg                                                  >  1:654427/1‑150 (MQ=255)

                                                                                                       ttgattgaTTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGc                                               <  1:1065123/150‑1 (MQ=255)

                                                                                                        tgattgatTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGcc                                              >  2:85968/1‑150 (MQ=255)

                                                                                                         gattgattGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCa                                             <  2:378371/150‑1 (MQ=255)

                                                                                                          tttgattgCTCAAGTAGTTAAATATGCATTAACATGGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACGACGTTTCCTCCTGTGCCGTATTTGTGCCAt                                            <  2:1131506/149‑1 (MQ=255)

                                                                                                           ttgattgCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGACGTATTTGTGCCAtt                                           <  2:869630/150‑1 (MQ=255)

                                                                                                             tattgCTCATGTAGTTAAAAATGCATTAACATCTCTTTCGTAATGCGAAGGTCGTAGGTTCGACTACTATTATCGTCACCATTAAAATCAAAGAGTTACCCGATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGt                                         <  1:254838/149‑1 (MQ=38)

                                                                                                             gattgCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGt                                         >  2:116554/1‑150 (MQ=255)

                                                                                                             gattgCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGt                                         >  2:433616/1‑150 (MQ=255)

                                                                                                                 gCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAAcc                                     <  1:636860/150‑1 (MQ=255)

                                                                                                                   tCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCtt                                   <  1:446131/150‑1 (MQ=255)

                                                                                                                    ctagtagtTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTg                                  <  1:1540996/148‑1 (MQ=255)

                                                                                                                    cAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTg                                  <  1:343343/150‑1 (MQ=255)

                                                                                                                      agtagtTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTGCCCCATATTTAAATACACGACGTTTCCTCCTGTGCCGGATTTGTGGCATTGTAGCCTTggg                                >  2:1583126/1‑149 (MQ=37)

                                                                                                                         agtTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTAACGGCACCATTAAAATAAAAGAGTTACCCAATATTTAAATACACAACGTTTCCTCCGGTGCCGTATTTGTGCCATTGTAACCTTGGCAAt                             >  1:939519/1‑150 (MQ=38)

                                                                                                                          gtTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACACCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAAtt                            >  1:562229/1‑150 (MQ=255)

                                                                                                                              aaaaTGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATtcatc                        >  1:1110614/1‑150 (MQ=255)

                                                                                                                                aaTGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCaa                      <  1:372711/150‑1 (MQ=255)

                                                                                                                                aaTGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCaa                      >  1:1286167/1‑150 (MQ=255)

                                                                                                                                   gCATTAACATCGCATTCGTAATGCGAAGGTCGTATGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAAt                   <  2:319347/150‑1 (MQ=255)

                                                                                                                                   gCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAAt                   <  1:433616/150‑1 (MQ=255)

                                                                                                                                   gCATTAACATCGCATTAGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCGTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAAt                   <  2:712047/150‑1 (MQ=255)

                                                                                                                                    cATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATa                  >  2:1582566/1‑150 (MQ=255)

                                                                                                                                     aTTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATAc                 <  1:843552/150‑1 (MQ=255)

                                                                                                                                     aTTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATAc                 >  1:1341114/1‑150 (MQ=255)

                                                                                                                                      ttAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATACt                >  2:1613596/1‑150 (MQ=255)

                                                                                                                                       tAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATACTg               <  1:190422/150‑1 (MQ=255)

                                                                                                                                        accttCGCATTACGAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATACTGt              >  1:735467/5‑150 (MQ=255)

                                                                                                                                        aaCATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATACTGt              <  1:1021227/150‑1 (MQ=255)

                                                                                                                                         ccttCGCATTACGAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATACTGtt             >  2:1232048/4‑150 (MQ=255)

                                                                                                                                          cATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACTCCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATACTGTTc            >  2:117916/1‑150 (MQ=255)

                                                                                                                                          cATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATAAAAATCAAAGAGTTACCCCAAATTTAAATACACCACGTTTCCTCATGTTCCGTATTTGTGCCATAGTAACCTTGGCAATTCATCAAAATACAGTTc            >  1:1720706/1‑150 (MQ=37)

                                                                                                                                           ttCGCATTACGAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCAATATTTAAATACACAAAGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATTCTGTTCt           >  2:1218232/2‑150 (MQ=37)

                                                                                                                                              gCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATACTGTTCTGAc        <  2:495620/150‑1 (MQ=255)

                                                                                                                                                   cGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTACCCTTGGCAATTCATCAAAATAATGTTCTGCCATCAg   >  1:206449/1‑150 (MQ=255)

                                                                                                                                                    gTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATACTGTTCTGACATCAgg  <  2:1415324/150‑1 (MQ=255)

TGCTGGTGTAAGTAAATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATACTGTTCTGACATCAGG  >  NC_000913/297065‑297362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`