Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,033,627 T→A 5.5% intergenic (+14/+30) idi → / ← lysS isopentenyl diphosphate isomerase/lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,033,6270TA 5.5% 332.6 / 4.2 116intergenic (+14/+30)idi/lysSisopentenyl diphosphate isomerase/lysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base T (58/51);  new base A (2/5);  total (60/56)
Fisher's exact test for biased strand distribution p-value = 2.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

TTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCA  >  NC_000913/3033478‑3033774
                                                                                                                                                     |                                                                                                                                                   
ttATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACAt                                                                                                                                                     >  1:759256/1‑150 (MQ=255)
 tATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACAtt                                                                                                                                                    <  1:423588/150‑1 (MQ=255)
 tATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACAtt                                                                                                                                                    >  1:1575183/1‑150 (MQ=255)
   tCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTg                                                                                                                                                  >  2:804021/1‑150 (MQ=255)
   tCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTg                                                                                                                                                  >  2:380964/1‑150 (MQ=255)
   tCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTg                                                                                                                                                  <  1:832960/150‑1 (MQ=255)
        ggTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCggg                                                                                                                                             <  1:1167374/150‑1 (MQ=255)
          tgtgATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGt                                                                                                                                           <  2:621073/150‑1 (MQ=255)
           gtgATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGtt                                                                                                                                          >  2:569809/1‑150 (MQ=255)
             gATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGGCAAATGTCGGGGtttt                                                                                                                                        <  1:1083252/150‑3 (MQ=255)
             gATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGGCAAATGTCGGGGtttt                                                                                                                                        <  2:506161/150‑3 (MQ=255)
                ttAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAg                                                                                                                                     <  2:1485097/150‑1 (MQ=255)
                  aGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCTGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCa                                                                                                                                   >  2:190309/1‑150 (MQ=255)
                  aGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCa                                                                                                                                   <  1:1269017/150‑1 (MQ=255)
                   gCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCAt                                                                                                                                  >  2:1086378/1‑150 (MQ=255)
                    cAGATGTATTACACGGTATTGATGCCACGCCGTGTGAGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATAGCGAAGCCAGAAAACGATTATATGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTGGTTAGCATa                                                                                                                                 >  1:1459177/1‑150 (MQ=255)
                    cAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATa                                                                                                                                 >  2:593200/1‑150 (MQ=255)
                        tGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGAAGGCGACAAATAGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGt                                                                                                                             >  1:1668209/1‑150 (MQ=255)
                             tACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGc                                                                                                                        >  2:652444/1‑150 (MQ=255)
                              acacGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCt                                                                                                                       <  2:941599/150‑1 (MQ=255)
                              acacGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCt                                                                                                                       >  1:409317/1‑150 (MQ=255)
                                     aTTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTa                                                                                                                >  1:261281/1‑150 (MQ=255)
                                       tGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTAcc                                                                                                              >  1:1145615/1‑150 (MQ=255)
                                       tGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTAcc                                                                                                              >  1:1367269/1‑150 (MQ=255)
                                       tGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACCTAATGCTTATTTTAcc                                                                                                              <  2:1182696/150‑1 (MQ=255)
                                        gATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCg                                                                                                             >  1:1568729/1‑150 (MQ=255)
                                          tGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGa                                                                                                           >  1:138521/1‑150 (MQ=255)
                                          tGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGa                                                                                                           >  1:12554/1‑150 (MQ=255)
                                            ccACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGTCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACg                                                                                                         >  2:139725/1‑150 (MQ=255)
                                            ccACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACg                                                                                                         >  2:1476827/1‑150 (MQ=255)
                                            ccACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAAAGCTTATTTTACCggccc                                                                                                         >  2:1552830/1‑147 (MQ=255)
                                                 ccGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCg                                                                                                    >  2:25600/1‑150 (MQ=255)
                                                 ccGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCg                                                                                                    <  1:1142497/150‑1 (MQ=255)
                                                   gTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGcc                                                                                                  <  1:569809/150‑1 (MQ=255)
                                                   gTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGcc                                                                                                  >  1:1297184/1‑150 (MQ=255)
                                                   gTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGcc                                                                                                  <  1:1371858/150‑1 (MQ=255)
                                                         gTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAAc                                                                                            <  2:1575183/150‑1 (MQ=255)
                                                         gTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAAc                                                                                            <  1:1402960/150‑1 (MQ=255)
                                                           tCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAg                                                                                          >  1:501600/1‑150 (MQ=255)
                                                             aGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGaa                                                                                        <  1:1476827/150‑1 (MQ=255)
                                                                    ggATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGTCATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTc                                                                                 <  1:1316017/150‑1 (MQ=255)
                                                                    ggATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTc                                                                                 <  1:1200404/150‑1 (MQ=255)
                                                                     gATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTcg                                                                                <  1:87418/150‑1 (MQ=255)
                                                                      aTGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTcgc                                                                               >  1:1542602/1‑150 (MQ=255)
                                                                      aTGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTcgc                                                                               >  2:864000/1‑150 (MQ=255)
                                                                         gTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGa                                                                            >  1:907434/1‑150 (MQ=255)
                                                                         gTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGa                                                                            >  2:355057/1‑150 (MQ=255)
                                                                           gATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATg                                                                          >  2:570778/1‑150 (MQ=255)
                                                                            aTGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCACAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg                                                                         <  2:12554/150‑1 (MQ=255)
                                                                             tGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGt                                                                        <  2:220173/150‑1 (MQ=255)
                                                                             tGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGt                                                                        >  1:1568787/1‑150 (MQ=255)
                                                                              gCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTa                                                                       >  2:1678481/1‑150 (MQ=255)
                                                                               cAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTAt                                                                      <  1:1703536/150‑1 (MQ=255)
                                                                               cAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTAt                                                                      >  2:1463098/1‑150 (MQ=255)
                                                                                  gCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGc                                                                   <  2:138521/150‑1 (MQ=255)
                                                                                   cGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCt                                                                  <  2:907434/150‑1 (MQ=255)
                                                                                   cGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGGTGTGAGCATAACGTAATGCTTATTATACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCt                                                                  >  1:161235/1‑150 (MQ=255)
                                                                                      cAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGtt                                                               >  2:1204417/1‑150 (MQ=255)
                                                                                      cAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGtt                                                               >  2:1581980/1‑150 (MQ=255)
                                                                                       aaaTCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATTGTATGGCTGTTg                                                              <  2:1668209/150‑1 (MQ=255)
                                                                                        aaTCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTgg                                                             >  2:223780/1‑150 (MQ=255)
                                                                                           cgcgAAGCCAGAAAAGCTTTATCTGCATTTAACCAGCTTAATTAATAAAACCCCGACATTTGCCGTGGTTGTGAGCATAACGTAATGCTTATTTTAACGGACGCATAGCCGGGAACAGAATAACGTCGCGGATGGTATTGCTGTTGGTGa                                                          <  1:106658/150‑1 (MQ=255)
                                                                                           cgcgAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGa                                                          <  1:223780/150‑1 (MQ=255)
                                                                                             cgAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAAc                                                        >  1:122725/1‑150 (MQ=255)
                                                                                               aaGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAg                                                      <  1:1463098/150‑1 (MQ=255)
                                                                                                aGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGc                                                     <  1:1086378/150‑1 (MQ=255)
                                                                                                 gCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCa                                                    <  1:1666308/150‑1 (MQ=255)
                                                                                                   cAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCAtt                                                  <  1:1268429/150‑1 (MQ=255)
                                                                                                   cAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTAATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCAtt                                                  >  2:1538622/1‑150 (MQ=255)
                                                                                                     gAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTAc                                                <  2:1367269/150‑1 (MQ=255)
                                                                                                       aaaCGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCa                                              >  1:506261/1‑150 (MQ=255)
                                                                                                         aCGATTATCTGCATTTACCCAGCTTAAATACAAAAACCCCGACATTTGCCGGTGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATa                                            <  1:1202504/150‑1 (MQ=255)
                                                                                                              tATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTc                                       >  1:327685/1‑150 (MQ=255)
                                                                                                               aTCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCg                                      <  1:190309/150‑1 (MQ=255)
                                                                                                                tCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGa                                     >  2:1315183/1‑150 (MQ=255)
                                                                                                                 cTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGAt                                    >  2:64406/1‑150 (MQ=255)
                                                                                                                   gCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATAc                                  <  1:1581980/150‑1 (MQ=255)
                                                                                                                    cATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATAcc                                 >  2:15551/1‑150 (MQ=255)
                                                                                                                    cATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATAcc                                 <  1:593200/150‑1 (MQ=255)
                                                                                                                     aTTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCa                                >  1:438861/1‑150 (MQ=255)
                                                                                                                        tACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAAtt                             >  2:376865/1‑150 (MQ=255)
                                                                                                                        tACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGAAGCATAGCCGGGAACAGAATAACGACGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACTGTCGATACCAAtt                             >  1:1184984/1‑150 (MQ=255)
                                                                                                                         aCCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTc                            >  2:1282338/1‑150 (MQ=255)
                                                                                                                          cccAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGACCAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTcc                           >  1:862943/1‑150 (MQ=255)
                                                                                                                           ccAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTccc                          <  2:1542602/150‑1 (MQ=255)
                                                                                                                           ccAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTccc                          <  1:1656241/150‑1 (MQ=255)
                                                                                                                           ccAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTccc                          <  2:507962/150‑1 (MQ=255)
                                                                                                                            cAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCa                         <  2:1652517/150‑1 (MQ=255)
                                                                                                                              gCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGa                       <  2:122725/150‑1 (MQ=255)
                                                                                                                               cTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTGGGTGAACAGCATTACCATACGGACGATGCCAATTCCCAGAc                      >  1:683510/1‑150 (MQ=255)
                                                                                                                                  aaataaaAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctg                   >  2:654415/1‑150 (MQ=255)
                                                                                                                                  aaataaaAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctg                   >  2:824837/1‑150 (MQ=255)
                                                                                                                                   aataaaAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctgc                  >  2:55744/1‑150 (MQ=255)
                                                                                                                                     gctcacaaCCCCGGCAAATGTCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCCATTCCCAGACctgctg                >  1:1313824/7‑150 (MQ=255)
                                                                                                                                       aaaaaaCCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTc              <  1:1282338/150‑1 (MQ=255)
                                                                                                                                        aaaaaCCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACCGCATTACCATACGGTCGATACCAATTCACAGACCTGCTGTcg             >  1:690850/1‑150 (MQ=255)
                                                                                                                                        aaaaaCCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcg             >  2:407501/1‑150 (MQ=255)
                                                                                                                                              cccGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTa       >  2:1358695/1‑150 (MQ=255)
                                                                                                                                                 gACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGc    <  1:25600/150‑1 (MQ=255)
                                                                                                                                                   cATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCa  <  1:307188/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TTATCAATGGTGTGATTTAGCAGATGTATTACACGGTATTGATGCCACGCCGTGGGCGTTCAGTCCGTGGATGGTGATGCAGGCGACAAATCGCGAAGCCAGAAAACGATTATCTGCATTTACCCAGCTTAAATAAAAAAACCCCGACATTTGCCGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCA  >  NC_000913/3033478‑3033774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: