Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 297,214 T→G 5.2% pseudogene (9/49 nt) ptwF → tRNA‑OTHER

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913297,2140TG 5.2% 416.2 / 9.3 135pseudogene (9/49 nt)ptwFtRNA‑OTHER
Reads supporting (aligned to +/- strand):  ref base T (69/59);  new base G (4/3);  total (73/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.91e-01

Only 100 of 137 total aligned reads displayed.

TGCTGGTGTAAGTAAATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATACTGTTCTGACATCAGG  >  NC_000913/297065‑297362
                                                                                                                                                     |                                                                                                                                                    
tgctgGTGTAAGTAAATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGt                                                                                                                                                      >  1:555989/1‑150 (MQ=255)
tgctgGTGTAAGTAAATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATAAAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAAAGCATTAACATCGGATTCGt                                                                                                                                                      >  1:78456/1‑150 (MQ=255)
  ctgGTGTAAGTAAATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTaa                                                                                                                                                    <  1:163182/150‑1 (MQ=255)
    ggTGTAAGTAAATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATg                                                                                                                                                  >  1:869630/1‑150 (MQ=255)
      tgtAAGTAAATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCg                                                                                                                                                >  1:495620/1‑150 (MQ=255)
        taagtaaATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGaa                                                                                                                                              >  2:1430101/1‑150 (MQ=255)
        taagtaaATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTACGAATGCGat                                                                                                                                              <  1:653379/150‑2 (MQ=255)
         aagtaaATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTACGAATGCGatg                                                                                                                                             <  1:1156357/150‑3 (MQ=255)
          agtaaATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAgg                                                                                                                                            >  1:1024459/1‑150 (MQ=255)
               aTGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTa                                                                                                                                       <  1:1531004/150‑1 (MQ=255)
                   aGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGtt                                                                                                                                   <  1:1114012/150‑1 (MQ=255)
                   aGGGAATAAAAATCAACTTTTGTTAATTTCAATCAACGGGTTTTTTGCTATAACTGGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGtt                                                                                                                                   <  1:840779/150‑1 (MQ=255)
                      gattAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGa                                                                                                                                <  2:330896/147‑1 (MQ=255)
                      gAATAACCATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGa                                                                                                                                >  1:1579871/1‑150 (MQ=255)
                      gAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGa                                                                                                                                >  1:1586290/1‑150 (MQ=255)
                      gAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGa                                                                                                                                >  2:372711/1‑150 (MQ=255)
                             cATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCtat                                                                                                                         >  2:474532/1‑150 (MQ=255)
                              aTCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCtatt                                                                                                                        >  2:480481/1‑150 (MQ=255)
                                     tttGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCa                                                                                                                 <  1:1692259/150‑1 (MQ=255)
                                       tGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGTGAAGGTCGTAGGTTCGACTCCTATTATCGGCAcc                                                                                                               <  1:1571368/150‑1 (MQ=255)
                                       tGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCAcc                                                                                                               <  1:751264/150‑1 (MQ=255)
                                       tGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCAcc                                                                                                               <  1:1430101/150‑1 (MQ=255)
                                        gTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATTTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCTCATTCGTAATGCGAATGTCGTAGGTTCGACTCCTATTATCGGCACCa                                                                                                              >  1:1040327/1‑150 (MQ=255)
                                        gTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTTGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCa                                                                                                              >  2:1479019/1‑150 (MQ=255)
                                            aTTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTaa                                                                                                          >  2:636860/1‑150 (MQ=255)
                                              ttCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTaaaa                                                                                                        >  2:1366460/1‑150 (MQ=255)
                                                 aatcaaCGGGTTTATTGCTATAACTTGGTTTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCa                                                                                                     <  1:1623311/150‑1 (MQ=255)
                                                 aatcaaCGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCa                                                                                                     <  1:474532/150‑1 (MQ=255)
                                                    caaCGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAag                                                                                                  <  2:131851/150‑1 (MQ=255)
                                                       cGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGt                                                                                               >  2:446131/1‑150 (MQ=255)
                                                         ggTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATTAATAAACATCTCATTCGTAATGCGAAGGTCGTAGGTTCGACTCATATTATCGGCACCATTAAAATCAAAAAGTTa                                                                                             >  1:1470322/1‑150 (MQ=37)
                                                         ggTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTa                                                                                             >  2:1065123/1‑150 (MQ=255)
                                                            ttATTGCTATAACTTGGTTTTATATAATATAAATTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTACGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTAccc                                                                                          <  2:1066750/150‑1 (MQ=255)
                                                            ttATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAGGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTAccc                                                                                          <  1:1605826/150‑1 (MQ=255)
                                                                   tataACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATAttt                                                                                   >  1:575967/1‑150 (MQ=255)
                                                                   tataACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATAttt                                                                                   >  2:1165747/1‑150 (MQ=255)
                                                                    ataACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTa                                                                                  >  2:1294051/1‑150 (MQ=255)
                                                                     taACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGAGAAGGTCGTAGGTTCGAATCATATTATCTGCACCATTAAAATCAAAGAGTTAACCCATATTTaa                                                                                 >  1:286636/1‑150 (MQ=255)
                                                                      aaCTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTaaa                                                                                >  1:726095/1‑150 (MQ=255)
                                                                      aaCTTGGGGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAACTGCATAAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTaaa                                                                                <  2:885815/150‑1 (MQ=255)
                                                                         ttGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATac                                                                             >  1:396436/1‑150 (MQ=255)
                                                                          tGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTAccc                                                                                          <  2:1678635/136‑1 (MQ=255)
                                                                          tGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATacac                                                                           <  2:1579871/150‑1 (MQ=255)
                                                                            gtgtTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATAcacc                                                                          >  2:882397/1‑150 (MQ=255)
                                                                             tgtTTTATAATATAAAGTATGTTGTTTTGATTGATTTCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGTTCGTAGGTTAGACTAATATTATCTGAACAATTAAAATAAAAGATTTACACAAAATTTAAAAacacaa                                                                         >  1:983837/1‑148 (MQ=255)
                                                                              gtTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATAcaccac                                                                        <  2:13117/150‑1 (MQ=255)
                                                                              gtTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCAAATTTAAATAcaccac                                                                        >  2:254838/1‑150 (MQ=255)
                                                                               ttttATAATATAAAGTATGTTGTTTTTATTGATTTCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACg                                                                       <  2:1486971/150‑1 (MQ=255)
                                                                                   ataatataaAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTtc                                                                   <  1:1158925/150‑1 (MQ=255)
                                                                                    taatataaAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTtcc                                                                  >  2:1021227/1‑150 (MQ=255)
                                                                                    taatataaAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTtcc                                                                  >  1:1699127/1‑150 (MQ=255)
                                                                                     aatataaAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCata                                                                                      >  2:74230/1‑129 (MQ=255)
                                                                                     aatataaAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCata                                                                                      <  1:74230/129‑1 (MQ=255)
                                                                                       tataaAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTtcctcc                                                               <  1:415663/150‑1 (MQ=255)
                                                                                        ataaAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTcctcct                                                              >  1:199902/1‑150 (MQ=255)
                                                                                         taaAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACCCCACGTTTCCTCCtg                                                             >  1:1491376/1‑150 (MQ=255)
                                                                                         taaAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCtg                                                             >  2:813127/1‑150 (MQ=255)
                                                                                         taaAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCAATATTTAAATACACCACGTTTCCTCCtg                                                             >  1:824271/1‑150 (MQ=255)
                                                                                           aaGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCtgtg                                                           >  2:843552/1‑150 (MQ=255)
                                                                                              tATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCg                                                        <  1:882397/150‑1 (MQ=255)
                                                                                              tATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCg                                                        <  2:457079/150‑1 (MQ=255)
                                                                                              tATGTTGTTGTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCTTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCg                                                        <  1:118823/150‑1 (MQ=255)
                                                                                               aTGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGt                                                       >  2:33960/1‑150 (MQ=255)
                                                                                                tgttgtTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTtcct                                                                 <  2:860878/139‑1 (MQ=255)
                                                                                                    gttTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACACCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTtg                                                  >  1:654427/1‑150 (MQ=255)
                                                                                                       ttgattgaTTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGc                                               <  1:1065123/150‑1 (MQ=255)
                                                                                                        tgattgatTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGcc                                              >  2:85968/1‑150 (MQ=255)
                                                                                                         gattgattGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCa                                             <  2:378371/150‑1 (MQ=255)
                                                                                                          tttgattgCTCAAGTAGTTAAATATGCATTAACATGGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACGACGTTTCCTCCTGTGCCGTATTTGTGCCAt                                            <  2:1131506/149‑1 (MQ=255)
                                                                                                           ttgattgCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGACGTATTTGTGCCAtt                                           <  2:869630/150‑1 (MQ=255)
                                                                                                             tattgCTCATGTAGTTAAAAATGCATTAACATCTCTTTCGTAATGCGAAGGTCGTAGGTTCGACTACTATTATCGTCACCATTAAAATCAAAGAGTTACCCGATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGt                                         <  1:254838/149‑1 (MQ=38)
                                                                                                             gattgCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGt                                         >  2:116554/1‑150 (MQ=255)
                                                                                                             gattgCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGt                                         >  2:433616/1‑150 (MQ=255)
                                                                                                                 gCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAAcc                                     <  1:636860/150‑1 (MQ=255)
                                                                                                                   tCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCtt                                   <  1:446131/150‑1 (MQ=255)
                                                                                                                    ctagtagtTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTg                                  <  1:1540996/148‑1 (MQ=255)
                                                                                                                    cAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTg                                  <  1:343343/150‑1 (MQ=255)
                                                                                                                      agtagtTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTGCCCCATATTTAAATACACGACGTTTCCTCCTGTGCCGGATTTGTGGCATTGTAGCCTTggg                                >  2:1583126/1‑149 (MQ=37)
                                                                                                                         agtTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTAACGGCACCATTAAAATAAAAGAGTTACCCAATATTTAAATACACAACGTTTCCTCCGGTGCCGTATTTGTGCCATTGTAACCTTGGCAAt                             >  1:939519/1‑150 (MQ=38)
                                                                                                                          gtTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACACCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAAtt                            >  1:562229/1‑150 (MQ=255)
                                                                                                                              aaaaTGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATtcatc                        >  1:1110614/1‑150 (MQ=255)
                                                                                                                                aaTGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCaa                      <  1:372711/150‑1 (MQ=255)
                                                                                                                                aaTGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCaa                      >  1:1286167/1‑150 (MQ=255)
                                                                                                                                   gCATTAACATCGCATTCGTAATGCGAAGGTCGTATGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAAt                   <  2:319347/150‑1 (MQ=255)
                                                                                                                                   gCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAAt                   <  1:433616/150‑1 (MQ=255)
                                                                                                                                   gCATTAACATCGCATTAGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCGTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAAt                   <  2:712047/150‑1 (MQ=255)
                                                                                                                                    cATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATa                  >  2:1582566/1‑150 (MQ=255)
                                                                                                                                     aTTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATAc                 <  1:843552/150‑1 (MQ=255)
                                                                                                                                     aTTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATAc                 >  1:1341114/1‑150 (MQ=255)
                                                                                                                                      ttAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATACt                >  2:1613596/1‑150 (MQ=255)
                                                                                                                                       tAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATACTg               <  1:190422/150‑1 (MQ=255)
                                                                                                                                        accttCGCATTACGAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATACTGt              >  1:735467/5‑150 (MQ=255)
                                                                                                                                        aaCATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATACTGt              <  1:1021227/150‑1 (MQ=255)
                                                                                                                                         ccttCGCATTACGAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATACTGtt             >  2:1232048/4‑150 (MQ=255)
                                                                                                                                          cATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACTCCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATACTGTTc            >  2:117916/1‑150 (MQ=255)
                                                                                                                                          cATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATAAAAATCAAAGAGTTACCCCAAATTTAAATACACCACGTTTCCTCATGTTCCGTATTTGTGCCATAGTAACCTTGGCAATTCATCAAAATACAGTTc            >  1:1720706/1‑150 (MQ=37)
                                                                                                                                           ttCGCATTACGAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCAATATTTAAATACACAAAGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATTCTGTTCt           >  2:1218232/2‑150 (MQ=37)
                                                                                                                                              gCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATACTGTTCTGAc        <  2:495620/150‑1 (MQ=255)
                                                                                                                                                   cGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTACCCTTGGCAATTCATCAAAATAATGTTCTGCCATCAg   >  1:206449/1‑150 (MQ=255)
                                                                                                                                                    gTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATACTGTTCTGACATCAgg  <  2:1415324/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TGCTGGTGTAAGTAAATGGAGGGAATAAACATCAACTTTTGTTAATTTCAATCAACGGGTTTATTGCTATAACTTGGTGTTTTATAATATAAAGTATGTTGTTTTGATTGATTGCTCAAGTAGTTAAAAATGCATTAACATCGCATTCGTAATGCGAAGGTCGTAGGTTCGACTCCTATTATCGGCACCATTAAAATCAAAGAGTTACCCCATATTTAAATACACCACGTTTCCTCCTGTGCCGTATTTGTGCCATTGTAACCTTGGCAATTCATCAAAATACTGTTCTGACATCAGG  >  NC_000913/297065‑297362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: