Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,057,145 G→C 6.8% intergenic (+157/+33) sibC → / ← serA sRNA antisense regulator of toxic IbsC protein/D‑3‑phosphoglycerate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,057,1450GC 6.8% 422.4 / 15.2 130intergenic (+157/+33)sibC/serAsRNA antisense regulator of toxic IbsC protein/D‑3‑phosphoglycerate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (49/71);  new base C (2/7);  total (52/78)
Fisher's exact test for biased strand distribution p-value = 4.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.93e-01

Only 100 of 134 total aligned reads displayed.

CAGCCCCATGCTGCCCTGCCATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGA  >  NC_000913/3056996‑3057294
                                                                                                                                                     |                                                                                                                                                     
cAGCCCCATGCTGCCCTGCCATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAg                                                                                                                                                       >  2:1381343/1‑150 (MQ=255)
 aGCCCCATGCTGCCCTGCCATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGt                                                                                                                                                      <  1:548091/150‑1 (MQ=255)
 aGCCCCATGCTGCCCTGCCATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTg                                                                                                                                                     <  2:1678811/150‑1 (MQ=255)
  gCCCCATGCTGCCCTGCCATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTg                                                                                                                                                     >  2:1696694/1‑150 (MQ=255)
  gCCCCATGCTGCCCTGCCATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTg                                                                                                                                                     >  2:270899/1‑150 (MQ=255)
     ccATGCTGCCCTGCCATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGAcc                                                                                                                                                  >  2:141242/1‑150 (MQ=255)
     ccATGCTGCCCTGCCATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGAcc                                                                                                                                                  >  2:705772/1‑150 (MQ=255)
     ccATGCTGCCCTGCCATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAACAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGAcc                                                                                                                                                  <  1:511668/150‑1 (MQ=255)
      cATGCTGCCCTGCCATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCt                                                                                                                                                 >  1:652926/1‑150 (MQ=255)
          gtgccctgccATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGccc                                                                                                                                             <  2:106926/149‑1 (MQ=255)
          ctgccctgccATCGTAAATCCCCATTAAATAACCACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGCCTCGCAAAGTAGAATGTGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGAAAGTCAGAGACCTGccc                                                                                                                                             >  2:1006780/1‑150 (MQ=255)
           tgccctgccATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGGGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGCCCg                                                                                                                                            <  1:769470/150‑1 (MQ=255)
           tgccctgccATCGTAAATCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGt                                                                                                                                           >  1:245194/1‑150 (MQ=255)
            gccctgccATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGCCCGt                                                                                                                                           <  2:659918/150‑1 (MQ=255)
            gccctgccATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGt                                                                                                                                           <  1:1357924/150‑1 (MQ=255)
             ccctgccATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGtt                                                                                                                                          >  1:954471/1‑150 (MQ=255)
             ccctgccATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGtt                                                                                                                                          >  1:1235233/1‑150 (MQ=255)
             ccctgccATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGtt                                                                                                                                          >  2:558603/1‑150 (MQ=255)
               ctgccATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGa                                                                                                                                        >  1:1165851/1‑150 (MQ=255)
               ctgccATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGa                                                                                                                                        >  2:963306/1‑150 (MQ=255)
                tgccATCGTAAATCCCCCTTAAATCAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGACTGCACAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGCCCGttttt                                                                                                                                       <  1:1609852/150‑4 (MQ=255)
                tgccATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGCCCGttttt                                                                                                                                       <  1:994686/150‑4 (MQ=255)
                 gccATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAGGTCACTGACTTGCCCGtttttt                                                                                                                                      <  1:902641/150‑5 (MQ=255)
                 gccATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAtt                                                                                                                                      <  1:862185/150‑1 (MQ=255)
                 cccATGGTAAAACCCCTTTAAATAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAttt                                                                                                                                     <  2:1341849/149‑1 (MQ=255)
                  ccATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAttt                                                                                                                                     >  2:1023104/1‑150 (MQ=255)
                     tCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCa                                                                                                                                  <  2:716214/150‑1 (MQ=255)
                     tCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCa                                                                                                                                  >  1:1328130/1‑150 (MQ=255)
                      cGTAAATCCCCATTAAATTAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCag                                                                                                                                 <  2:79242/150‑1 (MQ=255)
                      cGTAAATCCCCATTAAATAACCACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCag                                                                                                                                 <  2:299423/150‑1 (MQ=255)
                        tAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCagag                                                                                                                               <  1:549835/150‑1 (MQ=255)
                             ccccATTAAATAAACACAACGCATTGATCTGACTTTGATTTATATTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGCAGAAGGGATAAAAAAACGGGCAAGTCACTGACCTGCCCTTTGATGTTCAGAGAAggg                                                                                                                          >  1:79148/1‑150 (MQ=255)
                              cccATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAgggg                                                                                                                         <  1:141242/150‑1 (MQ=255)
                              cccATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGCCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAgggg                                                                                                                         >  2:831500/1‑150 (MQ=255)
                               ccATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGTAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGa                                                                                                                        >  1:773595/1‑150 (MQ=255)
                                cATTAAATAAACACACCGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGaa                                                                                                                       >  2:1079102/1‑150 (MQ=255)
                                 aTTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAAt                                                                                                                      <  1:1026931/150‑1 (MQ=255)
                                  ttAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAAtt                                                                                                                     <  1:1634372/150‑1 (MQ=255)
                                   taaataaaCACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCTACGCGGCAACGGTGTGGAGAAGGTATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTa                                                                                                                    <  2:693255/150‑1 (MQ=255)
                                    aattaaaCACAACGCTTTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTTGATTGCGCAACGCGGAATCGGTGTGGAGATGGGATATAAAAACGTGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGTATTAg                                                                                                                   <  1:1012187/147‑1 (MQ=255)
                                    aaataaaCACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAg                                                                                                                   >  1:1713114/1‑150 (MQ=255)
                                    aaataaaCACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGAGTTTCAGAGAAGGGGAATTAg                                                                                                                   >  2:588917/1‑150 (MQ=255)
                                      ataaaCACAACGCATTTATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAAAGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTAAGTGAACTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTa                                                                                                                 <  2:1290313/150‑1 (MQ=255)
                                       taaaCACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAc                                                                                                                <  2:685050/150‑1 (MQ=255)
                                       taaaCACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAc                                                                                                                <  1:353359/150‑1 (MQ=255)
                                         aaCACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAcag                                                                                                              >  1:875255/1‑150 (MQ=255)
                                          acacAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAcagc                                                                                                             <  2:996756/150‑1 (MQ=255)
                                           cacaACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAcagca                                                                                                            <  2:1032130/150‑1 (MQ=255)
                                            acaACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAcagcag                                                                                                           <  2:206081/150‑1 (MQ=255)
                                            acaACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTAcagcag                                                                                                           <  2:676622/150‑1 (MQ=255)
                                               aCGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACg                                                                                                        <  1:926639/150‑1 (MQ=255)
                                                cGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACgg                                                                                                       >  1:687970/1‑150 (MQ=255)
                                                cGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACgg                                                                                                       <  1:1023104/150‑1 (MQ=255)
                                                 gCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACggg                                                                                                      <  1:1567486/150‑1 (MQ=255)
                                                      gATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGa                                                                                                 >  2:649727/1‑150 (MQ=255)
                                                         gtGACTGTGATTTATTTTTTGGTTCAGACTCGCAATGTAGATTGCGCAACGCGGCAACGGGGTGGAGAAGGGATAAAATATCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATg                                                                                              <  2:284324/149‑1 (MQ=255)
                                                          tGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATgg                                                                                             <  2:164186/150‑1 (MQ=255)
                                                                 gATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCgg                                                                                      >  1:873845/1‑150 (MQ=255)
                                                                 gATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCgg                                                                                      <  1:1189271/150‑1 (MQ=255)
                                                                 gATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCgg                                                                                      >  1:946668/1‑150 (MQ=255)
                                                                       ttttCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGc                                                                                <  1:677814/150‑1 (MQ=255)
                                                                       ttttCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGc                                                                                <  2:1010030/150‑1 (MQ=255)
                                                                          tCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCttt                                                                             >  2:757920/1‑150 (MQ=255)
                                                                          tCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCttt                                                                             <  1:649727/150‑1 (MQ=255)
                                                                           cTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTc                                                                            <  1:963306/150‑1 (MQ=255)
                                                                            tGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCa                                                                           >  2:1361807/1‑150 (MQ=255)
                                                                            tGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCa                                                                           <  2:363928/150‑1 (MQ=255)
                                                                               aGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTg                                                                        >  1:408771/1‑150 (MQ=255)
                                                                                 cAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGcc                                                                      <  2:1682211/150‑1 (MQ=255)
                                                                                 cAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGcc                                                                      <  2:1435647/150‑1 (MQ=255)
                                                                                   gACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTg                                                                    <  2:245194/150‑1 (MQ=255)
                                                                                    aCTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGc                                                                   <  1:1696694/150‑1 (MQ=255)
                                                                                    aCTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATAAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGc                                                                   <  2:1328130/150‑1 (MQ=255)
                                                                                     cTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCa                                                                  <  1:156936/150‑1 (MQ=255)
                                                                                        gCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAATGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAgcg                                                               <  2:1169857/150‑1 (MQ=255)
                                                                                        gCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGGCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGCTACACGGAATAGCTTTCATTGCCTGCAgcg                                                               <  2:1492238/150‑1 (MQ=255)
                                                                                          aaaGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGCCCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCt                                                             >  2:1193935/1‑150 (MQ=255)
                                                                                          aaaGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCt                                                             >  2:1345171/1‑150 (MQ=255)
                                                                                               aGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTc                                                        <  1:777420/150‑1 (MQ=255)
                                                                                                 aaTGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTTATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCgg                                                      <  1:705772/150‑1 (MQ=255)
                                                                                                            cgGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTtcg                                           >  1:614844/1‑150 (MQ=255)
                                                                                                              gCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTtcgtc                                         <  2:1713114/150‑1 (MQ=255)
                                                                                                               cAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTtcgtcg                                        <  2:954471/150‑1 (MQ=255)
                                                                                                               cAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTtcgtcg                                        <  2:1235233/150‑1 (MQ=255)
                                                                                                                    gtgtGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTtc                                   >  1:1554573/1‑150 (MQ=255)
                                                                                                                      gtgGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTtcaa                                 >  1:44565/1‑150 (MQ=255)
                                                                                                                       tgGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGACATAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTtcaat                                <  2:353356/150‑1 (MQ=255)
                                                                                                                       tgGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTtcaat                                >  2:372930/1‑150 (MQ=255)
                                                                                                                         gagaAGGTATAAACCAACGGGCAAGTCAGGGACCTGCCCGTTGATTTTCAGAGAAGGCGATTTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTtcaatat                              <  2:109569/150‑1 (MQ=255)
                                                                                                                                 aTAAAAAAACGGGCAAGGCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAAcc                      <  2:942651/150‑1 (MQ=255)
                                                                                                                                    aaaaaaCGGGCAAGTCAGTGACCTGCCCGGTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTGTCGGTAACGTATTCGTCGGCTTCAATATCAATAACccca                   >  1:848864/1‑150 (MQ=255)
                                                                                                                                      aaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCCGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATa                 >  2:1314039/1‑150 (MQ=255)
                                                                                                                                      aaaaCGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCATCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAAACACATa                 >  2:1307142/1‑150 (MQ=255)
                                                                                                                                         aCGGGCAGGTCACTGACTTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAAcc              >  1:911581/1‑150 (MQ=255)
                                                                                                                                           gggCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCa            <  2:946668/150‑1 (MQ=255)
                                                                                                                                            ggCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATTGTACCCTGAATAGCTTTCATTGACTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCAt           <  1:217215/150‑1 (MQ=255)
                                                                                                                                            ggCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCAt           <  2:426960/150‑1 (MQ=255)
                                                                                                                                                 gTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTggg      <  1:831500/150‑1 (MQ=255)
                                                                                                                                                   cAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCg    <  2:853265/150‑1 (MQ=255)
                                                                                                                                                     gTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGa  <  2:687970/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAGCCCCATGCTGCCCTGCCATCGTAAATCCCCATTAAATAAACACAACGCATTGATCTGACTTTGATTTATTTTCTGGAGCAGACTCGCAAAGTAGAATGCGCAACGCGGCAACGGTGTGGAGAAGGGATAAAAAAACGGGCAAGTCAGTGACCTGCCCGTTGATTTTCAGAGAAGGGGAATTAGTACAGCAGACGGGCGCGAATGGTACCCGGAATAGCTTTCATTGCCTGCAGCGCTTTTTCGGCAACGTCTTCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGA  >  NC_000913/3056996‑3057294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: