Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,059,703 A→T 6.0% intergenic (‑2/‑50) yqfE ← / → argP pseudogene, LysR family/transcriptional regulator for arginine transport and DNA replication genes; replication initiation inhibitor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,059,7030AT 6.0% 279.4 / 2.6 94intergenic (‑2/‑50)yqfE/argPpseudogene, LysR family/transcriptional regulator for arginine transport and DNA replication genes; replication initiation inhibitor
Reads supporting (aligned to +/- strand):  ref base A (41/47);  new base T (4/2);  total (45/49)
Fisher's exact test for biased strand distribution p-value = 4.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAATAACGCCACGCCATTCAACACAACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAGCGCGCGGCACAAAAGCTGTGCATTACACAAT  >  NC_000913/3059555‑3059849
                                                                                                                                                    |                                                                                                                                                  
cAATAACGCCACGCCATTCAACACAACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACaaaa                                                                                                                                                   >  2:1284220/1‑150 (MQ=255)
cAATAACGCCACGCCATTCAACACAACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACaaaa                                                                                                                                                   >  2:870423/1‑150 (MQ=255)
     acgccacgccATTCAACACAGCCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATtgtg                                                                                                                                              >  1:1414139/1‑150 (MQ=255)
      cgccacgccaTTCAACACAACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAACTGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTgtgt                                                                                                                                             <  1:1526695/150‑1 (MQ=255)
      cgccacgccaTTCAACACAACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTgtgt                                                                                                                                             >  1:308360/1‑150 (MQ=255)
      cgccacgccaTTCAACACAACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGAGAAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATAAAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTgtgt                                                                                                                                             <  2:663216/150‑1 (MQ=255)
       gccacgccaTTCAACACAACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTc                                                                                                                                            >  2:1319946/1‑150 (MQ=255)
       cccacgccaTTCAACACAACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTc                                                                                                                                            <  2:1509668/149‑1 (MQ=255)
        ccacgccaTTCAACACAACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCa                                                                                                                                           >  1:1397351/1‑150 (MQ=255)
          acgccaTTCAACACAACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAATTTTGTGTCagg                                                                                                                                         <  1:126058/150‑3 (MQ=255)
           cgccaTTCAACACAACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCTCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAg                                                                                                                                        <  2:1107400/150‑1 (MQ=255)
           attcaTTCAACAGATCCACGGCGCGTACTCGCTGAGAGTTATGCATTTTCGGAAATCCCTTATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAg                                                                                                                                        <  2:859507/147‑1 (MQ=255)
            gccaTTCAACACCACCAGGGCGCGAACTCGCGGAGCGAAATGCATTAGCGCAAATCCCTGAGCTTTTAAGCTGCGCTTTTAACCACGGATACCAATGTTACCCGCCGACGGATTCGGGTTATGCAACCTGACTCAAAATTGGGTCAGAGt                                                                                                                                       >  2:108454/1‑150 (MQ=255)
             ccaTTCAACACAACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTg                                                                                                                                      >  2:515835/1‑150 (MQ=255)
              caTTCAACACAACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGc                                                                                                                                     >  2:885687/1‑150 (MQ=255)
                 tCAACACAACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAgg                                                                                                                                  >  1:1060904/1‑150 (MQ=255)
                       caACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAATTTTGTGTCaggt                                                                                                                                        <  1:1336571/138‑4 (MQ=255)
                       caACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAATTTTGTGTCaggt                                                                                                                                        >  2:1336571/1‑135 (MQ=255)
                       caACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAg                                                                                                                            <  1:1284220/150‑1 (MQ=255)
                            aGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCAttt                                                                                                                       >  1:960462/1‑150 (MQ=255)
                                 gctaaCTCGATGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATTTTACACGCCGACGTCTGTGGTATATGCAACCTGACACAAAATAGTGTCATAGTGCAGGATAAAGCATTTACCat                                                                                                                  <  2:1119261/147‑2 (MQ=255)
                                 gcgAACTAGCTGATCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACACGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAg                                                                                                                  <  2:1265157/150‑1 (MQ=255)
                                  cgAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAgg                                                                                                                 >  1:1027763/1‑150 (MQ=255)
                                  cgAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAgg                                                                                                                 <  1:1144669/150‑1 (MQ=255)
                                    aaCTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAg                                                                                                               <  1:979771/150‑1 (MQ=255)
                                        cGCTGAGCGAAATGCATTAGCGCAAATCCCTGCTCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGa                                                                                                           >  1:962704/1‑150 (MQ=255)
                                            gAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGacaac                                                                                                       <  2:427463/150‑1 (MQ=255)
                                               cGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGc                                                                                                    <  1:1522591/150‑1 (MQ=255)
                                               cGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTACCAGGAGCAGACAACAGCa                                                                                                   <  2:1543403/150‑1 (MQ=255)
                                                gAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCa                                                                                                   >  1:526969/1‑150 (MQ=255)
                                                gAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCa                                                                                                   >  1:531407/1‑150 (MQ=255)
                                                 aaaTGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCaa                                                                                                  >  2:1119033/1‑150 (MQ=255)
                                                  aaTGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAAt                                                                                                 <  2:701598/150‑1 (MQ=255)
                                                  aaTGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAAt                                                                                                 <  1:425053/150‑1 (MQ=255)
                                                   aTGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATg                                                                                                <  1:687216/150‑1 (MQ=255)
                                                    tGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGa                                                                                               >  1:435737/1‑150 (MQ=255)
                                                    tGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGa                                                                                               <  2:1653702/150‑1 (MQ=255)
                                                      cATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGaaa                                                                                             <  2:1577234/150‑1 (MQ=255)
                                                      aaTTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAAAGTTACCCGCCGACGGCTTCTGTATATGCAACCTGACACAAAATTGTGTCAAAGTGCAGGAAAAAGAATTAACCAGGAGCAGACAACAGCAATGaaa                                                                                             <  2:335959/149‑1 (MQ=255)
                                                        ttAGCGGAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGTCGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACg                                                                                           <  1:710839/150‑1 (MQ=255)
                                                          aGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGcc                                                                                         >  2:1216868/1‑150 (MQ=255)
                                                            cgcAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCg                                                                                       >  2:1312280/1‑150 (MQ=255)
                                                             gcAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCgg                                                                                      <  1:769364/150‑1 (MQ=255)
                                                                 aTCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTa                                                                                  >  2:1495759/1‑150 (MQ=255)
                                                                     cTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGa                                                                              >  2:1177016/1‑150 (MQ=255)
                                                                       gATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAAc                                                                            <  1:1178838/150‑1 (MQ=255)
                                                                        aTCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACa                                                                           <  1:1508088/150‑1 (MQ=255)
                                                                         tCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACAt                                                                          >  2:346414/1‑150 (MQ=255)
                                                                         tCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACAt                                                                          >  2:797817/1‑150 (MQ=255)
                                                                          cTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACAtt                                                                         <  2:666099/150‑1 (MQ=255)
                                                                                        ttttAACCACTGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATg                                                           <  2:537705/150‑1 (MQ=255)
                                                                                           tAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCTCTGGATGCgg                                                        >  1:829286/1‑150 (MQ=255)
                                                                                           tAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCgg                                                        >  1:1648227/1‑150 (MQ=255)
                                                                                             aCCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTg                                                      <  1:1119033/150‑1 (MQ=255)
                                                                                             aCCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTg                                                      <  1:1174634/150‑1 (MQ=255)
                                                                                             aCCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTg                                                      >  2:1074908/1‑150 (MQ=255)
                                                                                              ccACGGATACTAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGTCACAAAATTGTGTCATAGTGAAGGAAAAATCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGa                                                     <  2:367099/150‑1 (MQ=255)
                                                                                                aCGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATa                                                   >  1:23545/1‑150 (MQ=255)
                                                                                                  ggATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACg                                                 >  1:870240/1‑150 (MQ=255)
                                                                                                  ggATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACg                                                 <  1:1576766/150‑1 (MQ=255)
                                                                                                  ggATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACg                                                 <  1:438836/150‑1 (MQ=255)
                                                                                                  ggATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACg                                                 <  1:923443/150‑1 (MQ=255)
                                                                                                    aTACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTg                                               <  2:1397351/150‑1 (MQ=255)
                                                                                                     tACAAATGTTACCCGCCGACGGATTCGGTATCTGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCACTGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGa                                              <  1:153170/150‑1 (MQ=255)
                                                                                                       cAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAAc                                            <  1:1216868/150‑1 (MQ=255)
                                                                                                          aTGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAg                                         <  1:805040/150‑1 (MQ=255)
                                                                                                           tGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTTCAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAgg                                        <  2:1027763/150‑1 (MQ=255)
                                                                                                           tGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAgg                                        <  2:960462/150‑1 (MQ=255)
                                                                                                            gTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGa                                       <  2:1060904/150‑1 (MQ=255)
                                                                                                               aCCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACGGGATGCGGTGATACGTGAACGAGGAtgt                                    >  1:289120/1‑148 (MQ=255)
                                                                                                                cccGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACATCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTg                                   <  2:659710/150‑1 (MQ=255)
                                                                                                                cccGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTg                                   >  2:1542857/1‑150 (MQ=255)
                                                                                                                 ccgccgACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACATTCACTGGATGCGGTGATACGTGAACGAGGATTTGa                                  <  1:1312280/150‑1 (MQ=255)
                                                                                                                 ccgccgACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGa                                  >  2:1478490/1‑150 (MQ=255)
                                                                                                                  cgccgACGGCTTCTGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAGCATTTACCAGGCGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAgc                                <  2:1414139/150‑1 (MQ=255)
                                                                                                                   gccgACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAgc                                <  1:515835/150‑1 (MQ=255)
                                                                                                                        cGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAGcgcgcg                           >  2:790871/1‑150 (MQ=255)
                                                                                                                          gCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAGCGCGCGGc                         <  1:870423/150‑1 (MQ=255)
                                                                                                                           cTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACGAGGAGCAGACAACAGAAATGAAACTCCCGGACTACAGAACATTACAGGCACTGGATGATGTGATACGTGAACGAGGATTTGAGCGCGCGGca                        >  1:456408/1‑150 (MQ=255)
                                                                                                                           cTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAGCGCGCGGca                        >  1:1058800/1‑150 (MQ=255)
                                                                                                                           cTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAGCGCGCGGca                        <  1:1495759/150‑1 (MQ=255)
                                                                                                                           cTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAGCGCGCGGca                        <  1:589299/150‑1 (MQ=255)
                                                                                                                              cGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAGCGCGCGGCACaa                     >  2:635568/1‑150 (MQ=255)
                                                                                                                                gTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAGCGCGCGGCACaaaa                   >  1:545179/1‑150 (MQ=255)
                                                                                                                                   tatGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAGCGCGCGGCACAAAAGCt                >  2:178/1‑150 (MQ=255)
                                                                                                                                   tatGCAACCTGACACAAAATTGTCTCAAAGTGCAGCAAAAAGCATTTACCAGGAGCAGACATCAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACAGGATGAGGTGATACGTGAACGAGGATTTGATCGCGCGGCACCAAACCt                >  1:279762/1‑150 (MQ=255)
                                                                                                                                    atGCAACCTGCCACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACTCCCGGACTACAGAACATTACAGGCACTGGCTGCGGTCATACGTGACCGAGGAGTTGAGCGCGACTCACAAAAGCtg               >  1:325260/1‑150 (MQ=255)
                                                                                                                                    atGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAGCGCGCGGCACAAAAGCtg               <  1:1319946/150‑1 (MQ=255)
                                                                                                                                    atGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAGCGCGCGGCACAAAAGCtg               >  2:1439504/1‑150 (MQ=255)
                                                                                                                                      gCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAGCGCGCGGCACAAAAGCtgtg             <  1:885687/150‑1 (MQ=255)
                                                                                                                                       cactatGACACAATTTTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAGCGCGCGGCACAAAAGCTGTGc            >  2:169733/6‑150 (MQ=255)
                                                                                                                                         ctatGACACAATTTTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAGCGCGCGGCACAAAAGCTGTGCAt          >  1:1718272/4‑150 (MQ=255)
                                                                                                                                          ccTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAGCGCGCGGCACAAAAGCTGTGCAtt         >  1:414262/1‑150 (MQ=255)
                                                                                                                                           atGACACAATTTTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAGCGCGCGGCACAAAAGCTGTGCATTa        >  1:1582/2‑150 (MQ=255)
                                                                                                                                                 caAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAGCGCGCGGCACAAAAGCTGTGCATTACACAAt  >  1:1615886/1‑150 (MQ=255)
                                                                                                                                                 caAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAGCGCGCGGCACAAAAGCTGTGCATTACACAAt  >  2:799509/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CAATAACGCCACGCCATTCAACACAACCAGGGCGCGAACTCGCTGAGCGAAATGCATTAGCGCAAATCCCTGATCTTTTAAGCTGCGCTTTTAACCACGGATACAAATGTTACCCGCCGACGGCTTCGGTATATGCAACCTGACACAAAATTGTGTCATAGTGCAGGAAAAAGCATTTACCAGGAGCAGACAACAGCAATGAAACGCCCGGACTACAGAACATTACAGGCACTGGATGCGGTGATACGTGAACGAGGATTTGAGCGCGCGGCACAAAAGCTGTGCATTACACAAT  >  NC_000913/3059555‑3059849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: