Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,068,922 G→T 6.7% intergenic (‑114/+25) argO ← / ← mscS arginine transporter/mechanosensitive channel protein, small conductance

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,068,9220GT 6.7% 418.5 / 14.1 134intergenic (‑114/+25)argO/mscSarginine transporter/mechanosensitive channel protein, small conductance
Reads supporting (aligned to +/- strand):  ref base G (56/69);  new base T (2/7);  total (58/76)
Fisher's exact test for biased strand distribution p-value = 2.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.33e-01

Only 100 of 139 total aligned reads displayed.

CCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATCCCAGTACACGTTTTGCA  >  NC_000913/3068773‑3069071
                                                                                                                                                     |                                                                                                                                                     
cccAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCagag                                                                                                                                                       <  1:1663240/150‑1 (MQ=255)
 ccAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCagaga                                                                                                                                                      <  2:126603/150‑1 (MQ=255)
 ccAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCagaga                                                                                                                                                      <  2:1710983/150‑1 (MQ=255)
  cAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCagagag                                                                                                                                                     >  2:191464/1‑150 (MQ=255)
       gCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGtatta                                                                                                                                                >  1:347634/1‑150 (MQ=255)
       gCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGtatta                                                                                                                                                <  2:439512/150‑1 (MQ=255)
       gCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGtatta                                                                                                                                                >  2:705763/1‑150 (MQ=255)
       gCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGtatta                                                                                                                                                >  2:800558/1‑150 (MQ=255)
        cAAGACCTTGAAAGTAATAAGAAAAAACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTAAATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGCGAGGCCAGAGAGtattat                                                                                                                                               >  2:112369/1‑150 (MQ=255)
         aaGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATc                                                                                                                                              <  1:1633985/150‑1 (MQ=255)
          aGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCt                                                                                                                                             <  2:219871/150‑1 (MQ=255)
           gACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTg                                                                                                                                            <  1:414425/150‑1 (MQ=255)
            aCCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTgg                                                                                                                                           <  1:1590159/150‑1 (MQ=255)
            aCCTTGAAAGTAATAAGAAAACAAGTTGGATATTCCGTATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGTAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTgg                                                                                                                                           <  1:1392359/150‑1 (MQ=255)
              cTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATCTCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGcc                                                                                                                                         <  2:97183/150‑1 (MQ=255)
              cTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATTAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGcc                                                                                                                                         >  1:1618461/1‑150 (MQ=255)
              cTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGATAATACTCTCTGGcc                                                                                                                                         <  1:340994/150‑1 (MQ=255)
              cTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGATAATACTCTCTGGcc                                                                                                                                         <  2:829292/150‑1 (MQ=255)
                 gAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGATAATACTCTCTGGCCTct                                                                                                                                      <  1:787973/150‑2 (MQ=255)
                  aaaGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAg                                                                                                                                     >  2:269904/1‑150 (MQ=255)
                      taataaGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGtt                                                                                                                                 >  1:763735/1‑150 (MQ=255)
                       aataaGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTg                                                                                                                                <  2:1410883/150‑1 (MQ=255)
                       aataaGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTg                                                                                                                                >  2:521338/1‑150 (MQ=255)
                        ataaGAAAACAGGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCTATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGa                                                                                                                               <  1:1229723/150‑1 (MQ=255)
                          aaGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGAtt                                                                                                                             <  2:636566/150‑1 (MQ=255)
                          aaGAAAACACGTTGGATATTCAGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGAtt                                                                                                                             >  1:1121558/1‑150 (MQ=255)
                           aGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTAATATCTGGCCTCAGCTTTGACTa                                                                                                                            >  2:716742/1‑150 (MQ=255)
                           aGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTAATAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTa                                                                                                                            <  2:1619445/150‑1 (MQ=255)
                           aGAAAAAACGTTGGATATTCCGAATTAATTATCTCATGCATAGACTATATCTCGGGATTTTTATTAGCGGAAATAGATAATATTAATTGGAAATCAGAATAACTAATAAGCGAGAGGCCAGAGAGTAATATCTGTCCTCAGCGTTGATTa                                                                                                                            <  1:84199/150‑1 (MQ=255)
                            gAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTAc                                                                                                                           >  2:733178/1‑150 (MQ=255)
                            gAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGAGTAGATTaa                                                                                                                           >  1:1069706/1‑149 (MQ=255)
                               aaCACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCa                                                                                                                        <  2:317465/150‑1 (MQ=255)
                               aaCACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCa                                                                                                                        <  1:384442/150‑1 (MQ=255)
                                 cacGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGc                                                                                                                      >  1:1110685/1‑150 (MQ=255)
                                     tttgATATTCCGAATTAAGTATCTGATTCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTg                                                                                                                  <  2:820382/147‑1 (MQ=255)
                                     ttGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTg                                                                                                                  <  2:1647558/150‑1 (MQ=255)
                                       ggATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGtc                                                                                                                >  1:724983/1‑150 (MQ=255)
                                        gATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGtct                                                                                                               <  2:505877/150‑1 (MQ=255)
                                        gATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGtct                                                                                                               <  1:800558/150‑1 (MQ=255)
                                          tatTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGtcttc                                                                                                             <  1:1549268/150‑1 (MQ=255)
                                            ttCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTcttctt                                                                                                           >  2:725947/1‑150 (MQ=255)
                                             tCCGAATTAAGTATCTGCTGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCTGAGAGTATTATCTGGCCTCAGCGTGGATTCCGCAGCTTTGTcttctta                                                                                                          >  1:872658/1‑149 (MQ=255)
                                              gcGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGATATTAGCGGAATTTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGAATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTc                                                                                                         <  2:254864/149‑1 (MQ=255)
                                              ccGAATTAAGTATCTTATGCAGAGACTATATCTGGGGATTGTTATTAGCGGAAATTGATAATATTAATTGTCATTAAGAAAAACTATTTTGATATAGGCCAGAGATTATTATCTTGCCTGAGCGTTGATTACGCTGCTTTGTCTTCtttg                                                                                                         <  2:1610190/150‑2 (MQ=255)
                                              acGAATTAAGTATCTGATGCAGAGACTATATCGCGTGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTTATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTc                                                                                                         <  1:1130061/149‑1 (MQ=255)
                                                   ttAAGTATCTGATGCAGAGCCTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTTGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCg                                                                                                    <  2:349985/150‑1 (MQ=255)
                                                   ttAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCg                                                                                                    <  1:733178/150‑1 (MQ=255)
                                                       gTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTa                                                                                                <  2:194482/150‑1 (MQ=255)
                                                          tCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGATAATACTCTCTGGCCTctctct                                                                                                                                  <  1:1411021/113‑6 (MQ=38)
                                                          tCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGATAATACTCTCTGGCCTctctct                                                                                                                                  >  2:1411021/1‑108 (MQ=38)
                                                          tCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAg                                                                                             <  2:1310895/150‑1 (MQ=255)
                                                           cTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGt                                                                                            >  1:754925/1‑150 (MQ=255)
                                                                gCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTcaca                                                                                       <  1:1581092/150‑1 (MQ=255)
                                                                gCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTcaca                                                                                       <  1:499910/150‑1 (MQ=255)
                                                                 cAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACAt                                                                                      >  1:1527057/1‑150 (MQ=255)
                                                                  agagACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATc                                                                                     <  2:564502/150‑1 (MQ=255)
                                                                     gaCTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCAt                                                                                  >  1:1604516/1‑150 (MQ=255)
                                                                       cTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCAttt                                                                                >  2:1200304/1‑150 (MQ=255)
                                                                        tataTCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTg                                                                               <  2:499527/150‑1 (MQ=255)
                                                                          tatCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCg                                                                             >  2:275948/1‑150 (MQ=255)
                                                                             cgcgGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGt                                                                          >  2:1344525/1‑150 (MQ=255)
                                                                                gggATTGTTTTTAGCGGAAATTGATAATATTAATGGGCAATCAGAAAAACTAATAAGCGCGCGGCCAGCGACGATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACg                                                                       <  2:1084564/150‑1 (MQ=255)
                                                                                  gATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACggg                                                                     <  1:1496323/150‑1 (MQ=255)
                                                                                      gTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGc                                                                 <  2:1005659/150‑1 (MQ=255)
                                                                                      gTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGc                                                                 <  2:754925/150‑1 (MQ=255)
                                                                                      gTTAATAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGc                                                                 <  2:60856/150‑1 (MQ=255)
                                                                                         attaGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGa                                                              <  1:275948/150‑1 (MQ=255)
                                                                                           taGCGGAAATTGATAATATTAATTTGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATa                                                            >  2:1394677/1‑150 (MQ=255)
                                                                                           taGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATa                                                            <  2:1110685/150‑1 (MQ=255)
                                                                                            aGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATAc                                                           >  1:1081168/1‑150 (MQ=255)
                                                                                                gAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACcggc                                                       >  2:846681/1‑150 (MQ=255)
                                                                                                   aTTGATAATATTAATTTGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGATTTGTCATATTTAACCCGCTTAAAGTTCAAATCCATTTGCGGGTACGGGAAGCTGATACCgtcggc                                                    >  2:785572/1‑150 (MQ=255)
                                                                                                      gATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATc                                                 >  1:1283325/1‑150 (MQ=255)
                                                                                                            atTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTc                                           <  1:145237/150‑1 (MQ=255)
                                                                                                                 ttGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTCTTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGtt                                      <  1:269904/150‑1 (MQ=255)
                                                                                                                  tGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGttt                                     >  1:20246/1‑150 (MQ=255)
                                                                                                                  tGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGttt                                     >  2:1545214/1‑150 (MQ=255)
                                                                                                                     cAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAAt                                  >  1:922277/1‑150 (MQ=255)
                                                                                                                      aaTCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATa                                 >  2:882882/1‑150 (MQ=255)
                                                                                                                       aTCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATAc                                >  2:1579232/1‑150 (MQ=255)
                                                                                                                       aTCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTAAATAc                                >  1:391562/1‑150 (MQ=255)
                                                                                                                         cAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGc                              <  2:347634/150‑1 (MQ=255)
                                                                                                                         cAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGc                              <  1:705619/150‑1 (MQ=255)
                                                                                                                          aGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCt                             <  1:705763/150‑1 (MQ=255)
                                                                                                                          aGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCt                             <  2:1595213/150‑1 (MQ=255)
                                                                                                                          aGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCATTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCt                             <  2:330481/150‑1 (MQ=255)
                                                                                                                          aGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGACTCATCGTTGATTACTCAGCTTTGTATTCTTTCACAATATTAAAGTTCACATCAATTTTAGGTTACGTTAAGATTATAAAGTCGGCATAAAATTCACGTTTAATACGCt                             >  1:209561/1‑150 (MQ=255)
                                                                                                                           gAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTc                            >  2:466759/1‑150 (MQ=255)
                                                                                                                              aaaCTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAg                         <  2:1283325/150‑1 (MQ=255)
                                                                                                                                     taaGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATcc                  >  2:1581385/1‑150 (MQ=255)
                                                                                                                                      aaGAGAGAGGCCAGAGAGTATTATCTGGCCGCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCGGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATccc                 <  1:92213/150‑1 (MQ=255)
                                                                                                                                       agagagagGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATCCCa                >  2:1293160/1‑150 (MQ=255)
                                                                                                                                          gagagGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATCCCAGTa             <  1:1394677/150‑1 (MQ=255)
                                                                                                                                          ctgagGCCAGATAATACTCTCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATCCCAGTa             >  1:1076886/3‑150 (MQ=255)
                                                                                                                                               gCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATCCCAGTACACGt        >  1:1682417/1‑150 (MQ=255)
                                                                                                                                               gCCAGAGAGTATTATCTGGCCTCAGCCTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCTGCGGCATAAAATTCACGTTTAATTCGCTCCAGCACATCCCAGTACACGt        >  1:1571876/1‑150 (MQ=255)
                                                                                                                                                 cAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCTCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATCCCAGTACACGttt      <  1:29470/150‑1 (MQ=255)
                                                                                                                                                 cAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATCCCAGTACACGttt      >  1:1585557/1‑150 (MQ=255)
                                                                                                                                                 cAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATCCCAGTACACGttt      <  1:882882/150‑1 (MQ=255)
                                                                                                                                                     gagTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATCCCAGTACACGTTTTGCa  <  1:521338/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCCAAGTGCAAGACCTTGAAAGTAATAAGAAAACACGTTGGATATTCCGAATTAAGTATCTGATGCAGAGACTATATCGCGGGATTGTTATTAGCGGAAATTGATAATATTAATTGGCAATCAGAAAAACTAATAAGAGAGAGGCCAGAGAGTATTATCTGGCCTCAGCGTTGATTACGCAGCTTTGTCTTCTTTCACCCGCTTAAAGTTCACATCCATTTGCGGGTACGGGAAGCTGATACCGGCGGCATCAAATTCACGTTTAATACGCTCCAGCACATCCCAGTACACGTTTTGCA  >  NC_000913/3068773‑3069071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: