Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,105,536 G→C 7.7% intergenic (+24/‑178) mltC → / → nupG membrane‑bound lytic murein transglycosylase C/nucleoside transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,105,5360GC 7.7% 271.5 / 13.1 92intergenic (+24/‑178)mltC/nupGmembrane‑bound lytic murein transglycosylase C/nucleoside transporter
Reads supporting (aligned to +/- strand):  ref base G (37/47);  new base C (4/3);  total (41/51)
Fisher's exact test for biased strand distribution p-value = 6.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.17e-02

TATTATTAACACCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAAC  >  NC_000913/3105389‑3105681
                                                                                                                                                   |                                                                                                                                                 
tattatTAACACCATTACGCCGGGCTATGGTTATCAGAAGCTGAAGAACCGCCATCCCTCTGCGTAATCTCGCATGTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCt                                                                                                                                                 <  2:620928/150‑1 (MQ=255)
   tattaACACCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAg                                                                                                                                              >  1:1437702/1‑150 (MQ=255)
    attaACACCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAgg                                                                                                                                             <  2:768926/150‑1 (MQ=255)
      taACACCATTACGCCGGGCTATGTTTATCAGACGCTGACGACACGCGATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAATTACTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAgggg                                                                                                                                           <  2:1163454/150‑1 (MQ=255)
      taACACCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCAGACGATGCACAgggg                                                                                                                                           <  2:1323763/150‑1 (MQ=255)
       aaCACCATGACGCCGGGCGATGTTTATCACACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCCTTATCTTTATAAAGTGAATACCGCCCAAAAAACCAACCGCCGCCCAAAATTACATAAACCTCCACTGACGAAGCTCAGGTGc                                                                                                                                          >  1:348395/1‑150 (MQ=255)
        acacCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCa                                                                                                                                         <  1:1658062/150‑1 (MQ=255)
         cacCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGAGCATCGTCAGGGGcgg                                                                                                                                        <  2:924117/150‑3 (MQ=255)
         cacCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGAGCATCGTCAGGGGcgg                                                                                                                                        <  2:1537843/150‑3 (MQ=255)
         cacCAAGACGCCGGGTGATGTTTATCAGACGCTGACGTCCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCaa                                                                                                                                        <  1:1623452/150‑1 (MQ=255)
          acCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTTCGGAATCTCGCCGTTATCTTTATAAAGTGACTACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGAGCATCGTCAGGGGcggt                                                                                                                                       <  1:280080/150‑4 (MQ=255)
          acCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCaaa                                                                                                                                       <  1:1391123/150‑1 (MQ=255)
            cATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCaaaaa                                                                                                                                     <  2:1407485/150‑1 (MQ=255)
             aTGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCTTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTACATTAACCGCCCCTGACGATGCTCAGGGGCAAAAAt                                                                                                                                    <  1:1338242/150‑1 (MQ=255)
             aTGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAAt                                                                                                                                    <  2:313151/150‑1 (MQ=255)
             aTGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAAt                                                                                                                                    >  2:468009/1‑150 (MQ=255)
              tGACTCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAATTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATg                                                                                                                                   <  2:1640343/150‑1 (MQ=255)
                 cGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAACTCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGCCGATGCTCAGGGGCAAAAATGTTa                                                                                                                                >  2:732642/1‑150 (MQ=255)
                  gCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTAt                                                                                                                               >  2:781299/1‑150 (MQ=255)
                      ggCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAACAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGCCGATGCTCAGGGGCAAAAATGTGATCcac                                                                                                                           >  1:1466072/1‑150 (MQ=255)
                              ttATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAAt                                                                                                                   <  2:1398514/150‑1 (MQ=255)
                              ttATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAAt                                                                                                                   <  2:69948/150‑1 (MQ=255)
                                aTCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAAttt                                                                                                                 <  2:962546/150‑1 (MQ=255)
                                   aGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGACGTTATCTATATAAACTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCATGACGATGCTCAGGGCCAAACATGTTATCCACATCACAATTTcat                                                                                                              >  2:276650/1‑148 (MQ=255)
                                    gACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGtt                                                                                                             <  1:581043/150‑1 (MQ=255)
                                      cGCTGACGACCCGCCATCCCTGTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGtttt                                                                                                           <  2:700484/150‑1 (MQ=255)
                                       gCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTg                                                                                                          >  1:484297/1‑150 (MQ=255)
                                         tGACTACCCGCCATCCCTCTGCGGTTTCTCGCCGTTATCTTTATAAAGTGAATACCGCGCTAAAATCCTACCGCGGCCGATAATTCCATTAAGCCCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTGGTTTTGCa                                                                                                        <  2:348395/150‑1 (MQ=255)
                                          gacgacCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCaa                                                                                                       <  1:672573/150‑1 (MQ=255)
                                           acgacCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCaaa                                                                                                      <  1:1112969/150‑1 (MQ=255)
                                              acCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTg                                                                                                   >  1:1717200/1‑150 (MQ=255)
                                                  gccaaaccTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAATCCATTAACCGCCCCTGACGATGCTCAGGGGCAAGAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGTaa                                                                                               >  2:52503/7‑150 (MQ=255)
                                                      tCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGtt                                                                                           >  2:1567911/1‑150 (MQ=255)
                                                        ccTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATATTTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCCCATCACAATTTCGTTTTGCAAATTGGGAATGTTTg                                                                                         >  2:1096781/1‑150 (MQ=255)
                                                        ccTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTg                                                                                         <  2:1486742/150‑1 (MQ=255)
                                                        ccTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTg                                                                                         >  1:918879/1‑150 (MQ=255)
                                                         ctctGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGc                                                                                        >  2:1070494/1‑150 (MQ=255)
                                                             gCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAatt                                                                                    <  2:1588149/150‑1 (MQ=255)
                                                              ctgAATCTCTCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCATTGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAatta                                                                                   <  1:619668/148‑1 (MQ=255)
                                                              cGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAatta                                                                                   <  2:605279/150‑1 (MQ=255)
                                                               ggAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAattat                                                                                  >  1:926720/1‑150 (MQ=255)
                                                                  aTCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTg                                                                               >  2:20037/1‑150 (MQ=255)
                                                                    ctcGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGcc                                                                             <  2:713204/150‑1 (MQ=255)
                                                                      cGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCAAAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCcac                                                                           >  2:507189/1‑150 (MQ=255)
                                                                        ccGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAAATCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAg                                                                         <  2:483760/150‑1 (MQ=255)
                                                                         cGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAgg                                                                        <  2:744249/150‑1 (MQ=255)
                                                                            tATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTaa                                                                     >  1:412068/1‑150 (MQ=255)
                                                                              tCTTTATAAAGTTAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACa                                                                   <  1:20127/150‑1 (MQ=255)
                                                                               cTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACaa                                                                  >  2:1227481/1‑150 (MQ=255)
                                                                                tttATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACaaa                                                                 >  2:1382153/1‑150 (MQ=255)
                                                                                   ataAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACaaaaaa                                                              >  2:1294149/1‑150 (MQ=255)
                                                                                          gAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTcc                                                       <  1:1210849/150‑1 (MQ=255)
                                                                                             tACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCcgcg                                                    <  2:3820/150‑1 (MQ=255)
                                                                                                  agcAAAAATCCTACCGCCGCCGATAAATCCATTAACCGCACCTGACGATTCTCAGTGGCAATAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGtt                                               <  1:52503/149‑1 (MQ=255)
                                                                                                    cAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGa                                             >  1:673343/1‑150 (MQ=255)
                                                                                                    cAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGa                                             >  2:802888/1‑150 (MQ=255)
                                                                                                     aaaaaTCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGCCGATGCTCAGGGGCAAAAATGGTATCCACATCACAATATCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTgtt                                            >  1:121926/1‑148 (MQ=255)
                                                                                                     aaaaaTCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATGACAATTTCGTTTTGCAAATTGGGATTGTTTGCAAGTATGTGCCACAGGTAAAAAAAAACCAGTCCGAGAAGTTGAt                                            >  1:127361/1‑150 (MQ=255)
                                                                                                     aaaaaTCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGAt                                            <  1:1227481/150‑1 (MQ=255)
                                                                                                              aCCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCca                                   <  2:1363587/150‑1 (MQ=255)
                                                                                                              aCCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCca                                   <  1:1382153/150‑1 (MQ=255)
                                                                                                                 gccgccgATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCcatca                                >  2:401344/1‑150 (MQ=255)
                                                                                                                    gccgATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCAtct                             >  1:1067127/1‑150 (MQ=255)
                                                                                                                    gccgATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCAtct                             >  1:768151/1‑150 (MQ=255)
                                                                                                                     ccgATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCAtctc                            >  1:1395054/1‑150 (MQ=255)
                                                                                                                      cgATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCg                           <  2:8238/150‑1 (MQ=255)
                                                                                                                       gATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGc                          >  1:37498/1‑150 (MQ=255)
                                                                                                                         tAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCAc                        <  2:37498/150‑1 (MQ=255)
                                                                                                                           aTTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACgg                      >  1:1279374/1‑150 (MQ=255)
                                                                                                                           aTTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACgg                      <  1:1567911/150‑1 (MQ=255)
                                                                                                                            ttCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGt                     >  1:426617/1‑150 (MQ=255)
                                                                                                                                  tttttgCCCCTGAGCATCGTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAAtg               >  1:1513255/6‑150 (MQ=255)
                                                                                                                                  tAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAAtg               <  2:424230/150‑1 (MQ=255)
                                                                                                                                  tAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAAtg               <  2:484297/150‑1 (MQ=255)
                                                                                                                                    tttgCCCCTGAGCATCGTCAGGGGCGAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAAtgtg             >  1:1071529/4‑150 (MQ=255)
                                                                                                                                     cctccccTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGc            <  2:1549929/147‑1 (MQ=255)
                                                                                                                                     ccGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGc            <  2:509105/150‑1 (MQ=255)
                                                                                                                                     ccGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAAATATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGAGc            >  2:176951/1‑150 (MQ=255)
                                                                                                                                      cGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCt           <  1:1070494/150‑1 (MQ=255)
                                                                                                                                       gCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCtt          <  2:1717200/150‑1 (MQ=255)
                                                                                                                                       gCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCtt          >  1:379855/1‑150 (MQ=255)
                                                                                                                                       gCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGAGAAGTTGATAGAATCCCCTCATCTCGCACGGTCAAATGAGCtt          >  1:208186/1‑150 (MQ=255)
                                                                                                                                        ccccTGAGCATCGTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCttt         >  2:1193713/1‑150 (MQ=255)
                                                                                                                                        ccccTGAGCATCGTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCttt         >  1:514813/1‑150 (MQ=255)
                                                                                                                                        ccccTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGAGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCttt         >  1:96539/1‑150 (MQ=255)
                                                                                                                                        ccccTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGAGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCttt         <  2:96539/150‑1 (MQ=255)
                                                                                                                                          ccTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCttttt       >  2:870463/1‑150 (MQ=255)
                                                                                                                                           cTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTc      <  1:20037/150‑1 (MQ=255)
                                                                                                                                            tGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCa     <  2:412068/150‑1 (MQ=255)
                                                                                                                                               cGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAac  <  2:1466072/150‑1 (MQ=255)
                                                                                                                                               cGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAac  <  1:507189/150‑1 (MQ=255)
                                                                                                                                               cGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAac  <  1:389885/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TATTATTAACACCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCACATCACAATTTCGTTTTGCAAATTGGGAATGTTTGCAATTATTTGCCACAGGTAACAAAAAACCAGTCCGCGAAGTTGATAGAATCCCATCATCTCGCACGGTCAAATGTGCTTTTTCAAAC  >  NC_000913/3105389‑3105681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: