Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,107,879 G→T 6.5% A426D (GCT→GAT) ‡ speC ← ornithine decarboxylase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,107,8790GT 6.5% 330.4 / 9.8 107A426D (GCT→GAT) ‡speCornithine decarboxylase, constitutive
Reads supporting (aligned to +/- strand):  ref base G (46/54);  new base T (2/5);  total (48/59)
Fisher's exact test for biased strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.00e-01

Only 100 of 109 total aligned reads displayed.

ATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAGGCGTTATTCA  >  NC_000913/3107730‑3108026
                                                                                                                                                     |                                                                                                                                                   
atCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAg                                                                                                                                                     <  2:946766/150‑1 (MQ=255)
atCCTTCAAAGCCGTGACACTTCGCCACCGGCTCAAAACTGAAAAAACGGCGGTCGAGGGCTAACACTGATGTCGGATAATCCTGCCCCAATTTGCCATCAACAACGGGCGGGATAAACGGGGGGATCAGCTTACAGCGCGCAAGAATAg                                                                                                                                                     >  2:37232/1‑150 (MQ=255)
 tCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGc                                                                                                                                                    <  2:1242948/150‑1 (MQ=255)
 tCCTTCAAAGCCGTGCCACTGCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGc                                                                                                                                                    <  2:262522/150‑1 (MQ=255)
  ccTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGcc                                                                                                                                                   >  1:1294863/1‑150 (MQ=255)
       aaaGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTgcg                                                                                                                                              >  2:1165587/1‑150 (MQ=255)
        aaGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGGCTATTCTTgcgc                                                                                                                                             <  2:1533052/150‑1 (MQ=255)
        aaGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTgcgc                                                                                                                                             <  2:1263932/150‑1 (MQ=255)
          gCCGTGCCACTTTGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGGCTATTCTTgcgcgc                                                                                                                                           <  1:1319655/150‑1 (MQ=255)
          gCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGGCTATTCTTgcgcgc                                                                                                                                           <  1:657205/150‑1 (MQ=255)
            cGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCtt                                                                                                                                         <  2:352934/150‑1 (MQ=255)
                 cACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATc                                                                                                                                    <  1:911120/150‑1 (MQ=255)
                  aCTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGGCTATTCTTGCGCGCtgtaagct                                                                                                                                   <  2:460640/150‑8 (MQ=255)
                    ttCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTgcgc                                                                                                                                             <  1:698349/138‑1 (MQ=255)
                    ttCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTgcgc                                                                                                                                             >  2:698349/1‑138 (MQ=255)
                    ttCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCt                                                                                                                                 <  1:208093/150‑1 (MQ=255)
                     tCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTa                                                                                                                                <  1:712994/150‑1 (MQ=255)
                     tCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTa                                                                                                                                <  1:183841/150‑1 (MQ=255)
                        cccccGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGCTCTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTAtct                                                                                                                             <  1:90093/150‑1 (MQ=255)
                        cccccGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTAtct                                                                                                                             >  1:801864/1‑150 (MQ=255)
                         ccccGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACAGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTAtctc                                                                                                                            <  2:282095/150‑1 (MQ=255)
                          cccGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCa                                                                                                                           >  2:1700716/1‑150 (MQ=255)
                           ccGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAGCAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCATGAATAGCCTTGCGCGCTTCAATCCCTATCTCaa                                                                                                                          >  1:625713/1‑150 (MQ=255)
                           ccGGCTCAAAACTGAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAac                                                                                                                         <  2:1515282/150‑1 (MQ=255)
                             ggCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAaca                                                                                                                        <  2:621923/150‑1 (MQ=255)
                             ggCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTCCAATCCCTATCTCAaca                                                                                                                        <  1:1652114/150‑1 (MQ=255)
                                 cAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACt                                                                                                                    >  2:1016285/1‑150 (MQ=255)
                                  aaaaCTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTc                                                                                                                   >  1:790198/1‑150 (MQ=255)
                                     aCTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGc                                                                                                                <  1:1508672/150‑1 (MQ=255)
                                      cTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGcc                                                                                                               <  1:1393650/150‑1 (MQ=255)
                                      cTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGcc                                                                                                               <  2:27656/150‑1 (MQ=255)
                                        gAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATCAACGGGCGGAACAGCTTACAGCGCGCAAGCATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCca                                                                                                             >  1:48488/1‑150 (MQ=255)
                                        gAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCca                                                                                                             >  1:216593/1‑150 (MQ=255)
                                           aaaaCGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTT‑‑GAGCTTCAATCCCTATCTCAACACACTCAGCCCACAGcc                                                                                                        <  2:547322/150‑1 (MQ=255)
                                            aaaCGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGc                                                                                                         <  2:216593/150‑1 (MQ=255)
                                            aaaCGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGc                                                                                                         <  1:799637/150‑1 (MQ=255)
                                                 gcggTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACg                                                                                                    <  1:1097488/150‑1 (MQ=255)
                                                 gcggTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACg                                                                                                    >  1:733068/1‑150 (MQ=255)
                                                    gTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGccc                                                                                                 <  1:900073/150‑1 (MQ=255)
                                                        cTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTCTCTCAACACACTCAGCCCACAGCCGACGCCCActc                                                                                             >  1:674270/1‑150 (MQ=255)
                                                          ggCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACATCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGCCGCCCACtgtc                                                                                           >  1:1059356/1‑150 (MQ=255)
                                                          ggCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACtctc                                                                                           >  2:1181973/1‑150 (MQ=255)
                                                           gCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTcc                                                                                          >  2:58549/1‑150 (MQ=255)
                                                            cTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTccc                                                                                         <  1:972785/150‑1 (MQ=255)
                                                               acacTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCtt                                                                                      >  2:1665893/1‑150 (MQ=255)
                                                               acacTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCtt                                                                                      <  1:575194/150‑1 (MQ=255)
                                                                  cTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCAt                                                                                   <  1:1695528/150‑1 (MQ=255)
                                                                  cTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCAt                                                                                   <  1:523156/150‑1 (MQ=255)
                                                                   tGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATg                                                                                  >  1:1664142/1‑150 (MQ=255)
                                                                    gATGTCGGATAATCCTGCCTCATTTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGAGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACCCCCACACTCCACTTCATGa                                                                                 >  2:1201975/1‑150 (MQ=255)
                                                                    gATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGa                                                                                 >  2:1314070/1‑150 (MQ=255)
                                                                       gTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGACTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAAtt                                                                              >  1:110307/1‑150 (MQ=255)
                                                                         cGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAAtttt                                                                            >  1:1569096/1‑150 (MQ=255)
                                                                           gATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTgg                                                                          <  1:606223/150‑1 (MQ=255)
                                                                           gATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTgg                                                                          <  2:1024188/150‑1 (MQ=255)
                                                                           gATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTgg                                                                          <  2:553972/150‑1 (MQ=255)
                                                                              aaTCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATAAATTTTGGCGt                                                                       >  1:40971/1‑150 (MQ=255)
                                                                                tCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTa                                                                     <  2:1429177/150‑1 (MQ=255)
                                                                                 ccTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGCCGCCCACTCTCCCCTTCATGAATTTTGGCGTCaa                                                                    >  1:855514/1‑150 (MQ=255)
                                                                                  cTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCCATCCCTATCTCCACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAAc                                                                   >  1:258379/1‑150 (MQ=255)
                                                                                   tGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGCAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACa                                                                  <  1:561497/150‑1 (MQ=255)
                                                                                        caATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAg                                                             <  2:1294863/150‑1 (MQ=255)
                                                                                          aTTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCTTGAATTTTGGCGTTAACATCCAGTg                                                           >  1:939010/1‑150 (MQ=255)
                                                                                          aTTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTg                                                           <  1:1165587/150‑1 (MQ=255)
                                                                                             tGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTgcag                                                        >  1:1466187/1‑150 (MQ=255)
                                                                                             tGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTgcag                                                        <  1:1181973/150‑1 (MQ=255)
                                                                                             ggCCATCCACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTgcag                                                        <  2:935169/149‑1 (MQ=255)
                                                                                                cATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCaa                                                     >  2:496111/1‑150 (MQ=255)
                                                                                                  tCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAAc                                                   <  1:861624/150‑1 (MQ=255)
                                                                                                  tCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAAc                                                   <  1:1291077/150‑1 (MQ=255)
                                                                                                  acaaatacGTGCGGGAAAAACGGGCGGAACAGCTTACAGCGAGCAAGAATAGCCATGCGCGCTTCAATCCCTTTCTCTACACACTCAGCGCAGAGCCGACGCCCACTCTCCCCATCATGAATTTTGGCGTAAACATCCAGTGCAGCAAAc                                                   <  2:1070314/144‑1 (MQ=255)
                                                                                                     acaacGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCGTCATGATGATTGGGGTTAACATCCAGTGCAGCAAAccgg                                                >  2:189645/1‑147 (MQ=255)
                                                                                                      caacGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCACCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACATCg                                               >  2:308252/1‑150 (MQ=255)
                                                                                                      caacGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACACAGCCCACAGCCGACGCCCACTCTCACAGACATGAATTATCGCGTAAACATCCAGTGCAGCAAACAGcc                                               >  1:50425/1‑149 (MQ=255)
                                                                                                       aacGGGCGGGATAAACGGGCGGCACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCgg                                              >  1:273525/1‑150 (MQ=255)
                                                                                                          gggcgggATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCCCTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATa                                           >  1:1634027/1‑150 (MQ=255)
                                                                                                          gggcgggATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATa                                           >  2:95541/1‑150 (MQ=255)
                                                                                                              gggATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGaaa                                       >  1:225093/1‑150 (MQ=255)
                                                                                                                gATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCTACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAgg                                     <  1:1314070/150‑1 (MQ=255)
                                                                                                                   aaaCGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCt                                  <  2:1402801/150‑1 (MQ=255)
                                                                                                                      cGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTCTCTCAACACACTCAGCCCACAGCCGACGCCAACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGt                               <  2:674270/150‑1 (MQ=255)
                                                                                                                      cGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGt                               >  2:840771/1‑150 (MQ=255)
                                                                                                                      cGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGt                               <  2:674914/150‑1 (MQ=255)
                                                                                                                        ggCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAATGGCTGGTAg                             <  1:58549/150‑1 (MQ=255)
                                                                                                                            gAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGc                         >  2:1655458/1‑150 (MQ=255)
                                                                                                                                 gCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGa                    >  1:1052699/1‑150 (MQ=255)
                                                                                                                                 gCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTACCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGa                    >  2:1291856/1‑150 (MQ=255)
                                                                                                                                    tACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCa                 <  2:1569096/150‑1 (MQ=255)
                                                                                                                                      cAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATa               >  1:1394683/1‑150 (MQ=255)
                                                                                                                                      aaGCGCGCAAGGCTATTCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATa               >  1:1257449/2‑150 (MQ=255)
                                                                                                                                        gcgcgcAAGGCTATTCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATaaa             >  2:903984/1‑150 (MQ=255)
                                                                                                                                         cgcgcAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAg            >  1:1455287/1‑150 (MQ=255)
                                                                                                                                             cAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTATCATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAGGCGt        <  1:308252/150‑1 (MQ=255)
                                                                                                                                             cAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAGGCGt        >  2:223685/1‑150 (MQ=255)
                                                                                                                                              aaGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAGGCGtt       <  2:733068/150‑1 (MQ=255)
                                                                                                                                               aGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAATGGCTGGTAGAAGCATGGAGCATAAAGGCGTTa      <  1:1634785/150‑1 (MQ=255)
                                                                                                                                                 aaTAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAGGCGTTAtt    <  2:1466187/150‑1 (MQ=255)
                                                                                                                                                  aTAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATTAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAGGCGTTATTc   <  2:40971/150‑1 (MQ=255)
                                                                                                                                                   tAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCCGCCCACACCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAGGCGTTATTCa  <  2:48488/150‑1 (MQ=255)
                                                                                                                                                   tAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAGGCGTTATTCa  <  1:1016285/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
ATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAGGCGTTATTCA  >  NC_000913/3107730‑3108026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: