Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,107,880 C→T 6.8% A426T (GCT→ACT) ‡ speC ← ornithine decarboxylase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,107,8800CT 6.8% 320.2 / 7.9 103A426T (GCT→ACT) ‡speCornithine decarboxylase, constitutive
Reads supporting (aligned to +/- strand):  ref base C (44/51);  new base T (2/5);  total (47/56)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.35e-01

Only 100 of 107 total aligned reads displayed.

TCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAGGCGTTATTCA  >  NC_000913/3107731‑3108026
                                                                                                                                                     |                                                                                                                                                  
tCCTTCAAAGCCGTGCCACTTCGCCGCCGGCTCAAAACTGAAAAAACGGAGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCTTCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGc                                                                                                                                                    <  2:262525/150‑1 (MQ=255)
tCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGc                                                                                                                                                    <  2:1242948/150‑1 (MQ=255)
tCCTTCAAAGCCGTGCCACTGCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGc                                                                                                                                                    <  2:262522/150‑1 (MQ=255)
 ccTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGcc                                                                                                                                                   >  1:1294863/1‑150 (MQ=255)
      aaaGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTgcg                                                                                                                                              >  2:1165587/1‑150 (MQ=255)
       aaGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGGCTATTCTTgcgc                                                                                                                                             <  2:1533052/150‑1 (MQ=255)
       aaGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTgcgc                                                                                                                                             <  2:1263932/150‑1 (MQ=255)
         gCCGTGCCACTTTGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGGCTATTCTTgcgcgc                                                                                                                                           <  1:1319655/150‑1 (MQ=255)
         gCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGGCTATTCTTgcgcgc                                                                                                                                           <  1:657205/150‑1 (MQ=255)
         gCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGGCTATTCTTgcgcgc                                                                                                                                           <  1:544367/150‑1 (MQ=255)
           cGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGTCGAGATAAACGCGCGGAACAGCATACAGCGCGCAAGAATAGCCATGCGCGCtt                                                                                                                                         >  2:561497/1‑150 (MQ=255)
           cGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCtt                                                                                                                                         <  2:352934/150‑1 (MQ=255)
                cACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATc                                                                                                                                    <  1:911120/150‑1 (MQ=255)
                 aCTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGGCTATTCTTGCGCGCtgtaagct                                                                                                                                   <  2:460640/150‑8 (MQ=255)
                   ttCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTgcgc                                                                                                                                             >  2:698349/1‑138 (MQ=255)
                   ttCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTgcgc                                                                                                                                             <  1:698349/138‑1 (MQ=255)
                   ttCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCt                                                                                                                                 <  1:208093/150‑1 (MQ=255)
                    tCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTa                                                                                                                                <  1:183841/150‑1 (MQ=255)
                       cccccGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGCTCTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTAtct                                                                                                                             <  1:90093/150‑1 (MQ=255)
                       cccccGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTAtct                                                                                                                             >  1:801864/1‑150 (MQ=255)
                        ccccGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACAGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTAtctc                                                                                                                            <  2:282095/150‑1 (MQ=255)
                         cccGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCa                                                                                                                           >  2:1700716/1‑150 (MQ=255)
                          ccGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAGCAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCATGAATAGCCTTGCGCGCTTCAATCCCTATCTCaa                                                                                                                          >  1:625713/1‑150 (MQ=255)
                          ccGGCTCAAAACTGAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAac                                                                                                                         <  2:1515282/150‑1 (MQ=255)
                            ggCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAaca                                                                                                                        <  2:621923/150‑1 (MQ=255)
                            ggCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTCCAATCCCTATCTCAaca                                                                                                                        <  1:1652114/150‑1 (MQ=255)
                                cAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACt                                                                                                                    >  2:1016285/1‑150 (MQ=255)
                                 aaaaCTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTc                                                                                                                   >  1:790198/1‑150 (MQ=255)
                                    aCTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGc                                                                                                                <  1:1508672/150‑1 (MQ=255)
                                     cTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGcc                                                                                                               <  1:1393650/150‑1 (MQ=255)
                                     cTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGcc                                                                                                               <  2:27656/150‑1 (MQ=255)
                                       gAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATCAACGGGCGGAACAGCTTACAGCGCGCAAGCATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCca                                                                                                             >  1:48488/1‑150 (MQ=255)
                                       gAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCca                                                                                                             >  1:216593/1‑150 (MQ=255)
                                          aaaaCGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTT‑‑GAGCTTCAATCCCTATCTCAACACACTCAGCCCACAGcc                                                                                                        <  2:547322/150‑1 (MQ=255)
                                           aaaCGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGc                                                                                                         <  1:799637/150‑1 (MQ=255)
                                           aaaCGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGc                                                                                                         <  2:216593/150‑1 (MQ=255)
                                                gcggTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACg                                                                                                    <  1:1097488/150‑1 (MQ=255)
                                                gcggTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACg                                                                                                    >  1:733068/1‑150 (MQ=255)
                                                   gTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGccc                                                                                                 <  1:900073/150‑1 (MQ=255)
                                                       cTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTCTCTCAACACACTCAGCCCACAGCCGACGCCCActc                                                                                             >  1:674270/1‑150 (MQ=255)
                                                         ggCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACATCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGCCGCCCACtgtc                                                                                           >  1:1059356/1‑150 (MQ=255)
                                                         ggCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACtctc                                                                                           >  2:1181973/1‑150 (MQ=255)
                                                          gCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTcc                                                                                          >  2:58549/1‑150 (MQ=255)
                                                           cTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTccc                                                                                         <  1:972785/150‑1 (MQ=255)
                                                              acacTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCtt                                                                                      <  1:575194/150‑1 (MQ=255)
                                                              acacTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCtt                                                                                      >  2:1665893/1‑150 (MQ=255)
                                                                 cTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCAt                                                                                   <  1:1695528/150‑1 (MQ=255)
                                                                 cTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCAt                                                                                   <  1:523156/150‑1 (MQ=255)
                                                                  tGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATg                                                                                  >  1:1664142/1‑150 (MQ=255)
                                                                   gATGTCGGATAATCCTGCCTCATTTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGAGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACCCCCACACTCCACTTCATGa                                                                                 >  2:1201975/1‑150 (MQ=255)
                                                                   gATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGa                                                                                 >  2:1314070/1‑150 (MQ=255)
                                                                      gTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGACTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAAtt                                                                              >  1:110307/1‑150 (MQ=255)
                                                                        cGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAAtttt                                                                            >  1:1569096/1‑150 (MQ=255)
                                                                          gATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTgg                                                                          <  2:553972/150‑1 (MQ=255)
                                                                          gATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTgg                                                                          <  1:606223/150‑1 (MQ=255)
                                                                          gATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTgg                                                                          <  2:1024188/150‑1 (MQ=255)
                                                                             aaTCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATAAATTTTGGCGt                                                                       >  1:40971/1‑150 (MQ=255)
                                                                               tCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTa                                                                     <  2:1429177/150‑1 (MQ=255)
                                                                                ccTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGCCGCCCACTCTCCCCTTCATGAATTTTGGCGTCaa                                                                    >  1:855514/1‑150 (MQ=255)
                                                                                ccTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTaa                                                                    >  1:1328550/1‑150 (MQ=255)
                                                                                 cTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCCATCCCTATCTCCACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAAc                                                                   >  1:258379/1‑150 (MQ=255)
                                                                                 cTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAAc                                                                   >  2:1063120/1‑150 (MQ=255)
                                                                                  tGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGCAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACa                                                                  <  1:561497/150‑1 (MQ=255)
                                                                                       caATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAg                                                             <  2:1294863/150‑1 (MQ=255)
                                                                                        aaTTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGt                                                            <  2:1447698/150‑1 (MQ=255)
                                                                                         aTTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCTTGAATTTTGGCGTTAACATCCAGTg                                                           >  1:939010/1‑150 (MQ=255)
                                                                                         aTTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTg                                                           <  1:1165587/150‑1 (MQ=255)
                                                                                            tGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTgcag                                                        <  1:1181973/150‑1 (MQ=255)
                                                                                            tGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTgcag                                                        >  1:1466187/1‑150 (MQ=255)
                                                                                            ggCCATCCACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTgcag                                                        <  2:935169/149‑1 (MQ=255)
                                                                                               cATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCaa                                                     >  2:496111/1‑150 (MQ=255)
                                                                                                 tCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAAc                                                   <  1:1291077/150‑1 (MQ=255)
                                                                                                 tCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAAc                                                   <  1:861624/150‑1 (MQ=255)
                                                                                                   aacaacGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAg                                                 >  1:177516/1‑150 (MQ=255)
                                                                                                    acaacGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCGTCATGATGATTGGGGTTAACATCCAGTGCAGCAAAccgg                                                >  2:189645/1‑147 (MQ=255)
                                                                                                     caacGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCACCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACATCg                                               >  2:308252/1‑150 (MQ=255)
                                                                                                     caacGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACACAGCCCACAGCCGACGCCCACTCTCACAGACATGAATTATCGCGTAAACATCCAGTGCAGCAAACAGcc                                               >  1:50425/1‑149 (MQ=255)
                                                                                                         gggcgggATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCCCTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATa                                           >  1:1634027/1‑150 (MQ=255)
                                                                                                         gggcgggATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATa                                           >  2:95541/1‑150 (MQ=255)
                                                                                                             gggATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGaaa                                       >  1:225093/1‑150 (MQ=255)
                                                                                                               gATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCTACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAgg                                     <  1:1314070/150‑1 (MQ=255)
                                                                                                                  aaaCGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCt                                  <  2:1402801/150‑1 (MQ=255)
                                                                                                                     cGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTCTCTCAACACACTCAGCCCACAGCCGACGCCAACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGt                               <  2:674270/150‑1 (MQ=255)
                                                                                                                     cGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGt                               >  2:840771/1‑150 (MQ=255)
                                                                                                                     cGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGt                               <  2:674914/150‑1 (MQ=255)
                                                                                                                       ggCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAATGGCTGGTAg                             <  1:58549/150‑1 (MQ=255)
                                                                                                                           gAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGc                         >  2:1655458/1‑150 (MQ=255)
                                                                                                                                gCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGa                    >  1:1052699/1‑150 (MQ=255)
                                                                                                                                gCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTACCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGa                    >  2:1291856/1‑150 (MQ=255)
                                                                                                                                     cAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATa               >  1:1394683/1‑150 (MQ=255)
                                                                                                                                     aaGCGCGCAAGGCTATTCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATa               >  1:1257449/2‑150 (MQ=255)
                                                                                                                                       gcgcgcAAGGCTATTCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATaaa             >  2:903984/1‑150 (MQ=255)
                                                                                                                                            cAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTATCATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAGGCGt        <  1:308252/150‑1 (MQ=255)
                                                                                                                                            cAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAGGCGt        >  2:223685/1‑150 (MQ=255)
                                                                                                                                             aaGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAGGCGtt       <  2:733068/150‑1 (MQ=255)
                                                                                                                                              agcaTAGCCTTGCGCGCTTCAATCCCTATCTCAACACCCTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATTAAATTTGGCGTTAACATCCAGTGGAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAGGCGTTa      <  1:795465/147‑1 (MQ=255)
                                                                                                                                              aGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAATGGCTGGTAGAAGCATGGAGCATAAAGGCGTTa      <  1:1634785/150‑1 (MQ=255)
                                                                                                                                                aaTAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAGGCGTTAtt    <  2:1466187/150‑1 (MQ=255)
                                                                                                                                                 aTAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATTAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAGGCGTTATTc   <  2:40971/150‑1 (MQ=255)
                                                                                                                                                  tAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAGGCGTTATTCa  <  1:1016285/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAGGCGTTATTCA  >  NC_000913/3107731‑3108026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: