Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,111,961 T→C 5.0% pseudogene (130/963 nt) yghE ← pseudogene, secretion pathway protein, L‑type protein homology; putative general secretion pathway for protein export (GSP)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,111,9610TC 5.0% 358.8 / 6.8 120pseudogene (130/963 nt)yghEpseudogene, secretion pathway protein, L‑type protein homology; putative general secretion pathway for protein export (GSP)
Reads supporting (aligned to +/- strand):  ref base T (56/57);  new base C (3/3);  total (59/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

Only 100 of 121 total aligned reads displayed.

CGCCAGCCCGGCGCTCCATCCCCGGCATTCATCGATGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGCGGGCAGAATAGTCGGTAGCGCAGCGCGATGGAAAT  >  NC_000913/3111815‑3112110
                                                                                                                                                  |                                                                                                                                                     
cgccagcCCGGCGCTCCATCCCCGGCATTCATCGATGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGtt                                                                                                                                                    <  2:1295999/150‑1 (MQ=255)
 gccagcCCGGCGCTCCATCCCCGGCATTCATCGATGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTg                                                                                                                                                   <  2:1162450/150‑1 (MQ=255)
   cagcCCGGCGCTCCATCCCCGGCATTCATCGATGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTgcg                                                                                                                                                 <  1:1312733/150‑1 (MQ=255)
    agcCCGGCGCTCCATCCCCGGCATTCATCGATGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTgcgc                                                                                                                                                <  2:1090456/150‑1 (MQ=255)
    agcCCGGCGCTCCATCCCCGGCATTCATCGATGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAACATTgcgc                                                                                                                                                <  1:104272/150‑1 (MQ=255)
    agcCCGGCGCTCCATCCCCGGCATTCATCGATGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAACGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTgcgc                                                                                                                                                <  2:594190/150‑1 (MQ=255)
        cGGCGCTCCATCCCCGGCATTCATCGATGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCatat                                                                                                                                            <  1:1111973/150‑1 (MQ=255)
        cGGCGCTCCATCCCCGGCATTCATCGATGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAACATTGCGCagtg                                                                                                                                            <  2:1102284/150‑4 (MQ=255)
            gcTCCATCCCCGGCATTCATCGATGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTg                                                                                                                                        >  2:1080068/1‑150 (MQ=255)
            gcTCCATCCCCGGCATTCATCGATGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAACATTGCGCagtggttg                                                                                                                                        <  1:178270/150‑8 (MQ=255)
               ccATCCCCGGCATTCATCGATGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTg                                                                                                                                     <  1:175213/150‑1 (MQ=255)
                cATCCCCGGCATTCATCGATGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTgg                                                                                                                                    <  1:718659/150‑1 (MQ=255)
                cATCCCCGGCATTCATCGATGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTgg                                                                                                                                    <  1:339965/150‑1 (MQ=255)
                   ccccGGCATTCATCGATGCGGCAGATAACCCGTTGCTCATCGCCAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGAGCATATGCTGTTGGtta                                                                                                                                 >  2:1047802/1‑148 (MQ=255)
                         cATTCATCGATGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACt                                                                                                                           >  2:1079132/1‑150 (MQ=255)
                          aTTCATCGATGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTg                                                                                                                          <  1:1183903/150‑1 (MQ=255)
                            tCATCGATGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCt                                                                                                                        >  1:611765/1‑150 (MQ=255)
                             cATCGATGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGAGCATATGCTGTTGGTGAATCACTGCTa                                                                                                                       >  1:735636/1‑150 (MQ=255)
                                cGATGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCATTCCGGCGCGACGGTAGCGCGGGTGAAGCCGTATTCCTGCAACCACTGCGCAATGTTTCGCATATGCTTTTGGTGAATCACTGCTACgg                                                                                                                    >  2:623602/1‑150 (MQ=255)
                                  aTGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGtt                                                                                                                  <  2:230403/150‑1 (MQ=255)
                                          gATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTg                                                                                                          >  2:1446092/1‑150 (MQ=255)
                                          gATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTg                                                                                                          <  2:1569102/150‑1 (MQ=255)
                                           aTAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGc                                                                                                         >  1:1462397/1‑150 (MQ=255)
                                            tAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACCTCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCt                                                                                                        <  2:801275/150‑1 (MQ=255)
                                             aaCCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTg                                                                                                       >  2:773903/1‑150 (MQ=255)
                                              aCCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTGGCTgg                                                                                                      >  1:1453076/1‑150 (MQ=255)
                                               cccGTTGCGCATCGCAAGCCATACACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGt                                                                                                     >  2:107410/1‑150 (MQ=255)
                                               cccGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGt                                                                                                     <  2:669777/150‑1 (MQ=255)
                                               cccGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGt                                                                                                     <  2:897981/150‑1 (MQ=255)
                                               cccGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGt                                                                                                     >  1:537513/1‑150 (MQ=255)
                                                 cGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCg                                                                                                   <  2:494127/150‑1 (MQ=255)
                                                        cATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCa                                                                                            >  1:839255/1‑150 (MQ=255)
                                                          tCGCAAGCCATACACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTTCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCTGTTGCTGGTCGATATTCAAc                                                                                          >  1:817147/1‑150 (MQ=255)
                                                             cAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACggg                                                                                       >  1:677741/1‑150 (MQ=255)
                                                               aGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGc                                                                                     <  1:1080068/150‑1 (MQ=255)
                                                               aGCCATAAACCCACAAGGAAGGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGGCGATATTCAACGGGGc                                                                                     <  2:155118/150‑1 (MQ=255)
                                                                gCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCg                                                                                    >  1:901655/1‑150 (MQ=255)
                                                                gCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCg                                                                                    <  2:1199157/150‑1 (MQ=255)
                                                                 ccATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGa                                                                                   >  1:1411025/1‑150 (MQ=255)
                                                                 ccATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGa                                                                                   >  2:184133/1‑150 (MQ=255)
                                                                 ccATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGATGGTCGATATTCAACGGGGCGa                                                                                   >  2:1320232/1‑150 (MQ=255)
                                                                    tAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAAt                                                                                <  1:899266/150‑1 (MQ=255)
                                                                    aaaaCCCACAAGGAATGTACATCCAGTCCGGCGCGACGGAAGCGCGGGTGATGCAGGTTTCCTGCAACCACTGCGCAAGGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAAt                                                                                <  2:846193/149‑1 (MQ=255)
                                                                      aaCCCACAAGGAATGGACATCCCGTCCGGCGAGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGGTGCTGGTCGATATTCAACGGGGCGAAATGc                                                                              <  2:1649590/150‑1 (MQ=255)
                                                                        cccACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGCTGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAg                                                                            >  1:1573712/1‑150 (MQ=255)
                                                                        cccACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAg                                                                            >  1:739308/1‑150 (MQ=255)
                                                                        cccACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAg                                                                            >  1:369866/1‑150 (MQ=255)
                                                                        cccACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACAGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAg                                                                            >  2:1617284/1‑150 (MQ=255)
                                                                         ccACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGt                                                                           >  1:123890/1‑150 (MQ=255)
                                                                         ccACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGt                                                                           <  2:1459084/150‑1 (MQ=255)
                                                                         ccACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGt                                                                           <  1:1079132/150‑1 (MQ=255)
                                                                         ccACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCAAAATGCAGt                                                                           >  1:1456615/1‑150 (MQ=255)
                                                                          cacaAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGtt                                                                          >  2:405040/1‑150 (MQ=255)
                                                                            caAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCa                                                                        >  2:735696/1‑150 (MQ=255)
                                                                              aGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATc                                                                      <  2:677741/150‑1 (MQ=255)
                                                                               ggAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTTATGCCGTTTTCCTGCAATCTCTTCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCg                                                                     <  2:451587/150‑1 (MQ=255)
                                                                               ggAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCg                                                                     <  1:773903/150‑1 (MQ=255)
                                                                                gAATGTACATCCAGTCGGGCGCGAAGGTAGCGCGGGTGATGCAGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGa                                                                    <  2:839255/150‑1 (MQ=255)
                                                                                  aTGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGata                                                                  <  1:665290/150‑1 (MQ=255)
                                                                                  aTGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGata                                                                  <  1:481355/150‑1 (MQ=255)
                                                                                    ggACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATc                                                                <  1:1620567/150‑1 (MQ=255)
                                                                                    ggACATCCAGTCCGGCGCGACGGTAGAGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGGGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATc                                                                <  2:803167/150‑1 (MQ=255)
                                                                                        aTCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGCTATCCTgg                                                            >  1:539273/1‑150 (MQ=255)
                                                                                        aTCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTgg                                                            <  2:740695/150‑1 (MQ=255)
                                                                                        aTCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGATGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTgg                                                            <  2:204646/150‑1 (MQ=255)
                                                                                         tCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGt                                                           >  1:1606173/1‑150 (MQ=255)
                                                                                         tCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGt                                                           >  2:1684881/1‑150 (MQ=255)
                                                                                          ccAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGtt                                                          >  2:1596152/1‑150 (MQ=255)
                                                                                          ccAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGATGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGtt                                                          <  1:873041/150‑1 (MQ=255)
                                                                                             gTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAg                                                       >  2:47879/1‑150 (MQ=255)
                                                                                                cGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGctc                                                    >  2:1146033/1‑150 (MQ=255)
                                                                                                   cgcgACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCtcttc                                                 >  2:1454454/1‑150 (MQ=255)
                                                                                                    gcgACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCtcttct                                                >  1:1659238/1‑150 (MQ=255)
                                                                                                       aCGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTcc                                             <  2:1376907/150‑1 (MQ=255)
                                                                                                        cGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTgc                                                                                                                                                  <  1:1177697/48‑1 (MQ=255)
                                                                                                        cGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTgc                                                                                                                                                  <  1:1184192/48‑1 (MQ=255)
                                                                                                        cGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTgc                                                                                                                                                  >  2:1177697/1‑48 (MQ=255)
                                                                                                          gTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGt                                          >  2:1407996/1‑150 (MQ=255)
                                                                                                            aGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTaa                                        <  1:107410/150‑1 (MQ=255)
                                                                                                               gcgGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGc                                     >  1:948648/1‑150 (MQ=255)
                                                                                                                cgGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGCg                                    <  1:184133/150‑1 (MQ=255)
                                                                                                                  ggTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGCggg                                  >  1:1648445/1‑150 (MQ=255)
                                                                                                                   gTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGCGGGc                                 <  1:482643/150‑1 (MQ=255)
                                                                                                                        gCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGCGGGCAGAAt                            <  2:1606173/150‑1 (MQ=255)
                                                                                                                             tttCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGCGGGCAGAATAGTCg                       <  1:859274/150‑1 (MQ=255)
                                                                                                                             tttCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGCGGGCAGAATAGTCg                       <  2:901655/150‑1 (MQ=255)
                                                                                                                              ttCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGCGGGCAGATTAGTCgg                      >  1:25170/1‑150 (MQ=255)
                                                                                                                                 cTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGCGGGCAGAATAGTCGGTag                   <  2:308710/150‑1 (MQ=255)
                                                                                                                                  tGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGCGGGCAGAATAGTCGGTagc                  >  1:900902/1‑150 (MQ=255)
                                                                                                                                  tGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGCGGGCAGAATAGTCGGTagc                  >  2:1230588/1‑150 (MQ=255)
                                                                                                                                  tGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGCGGGCAGAATAGTCGGTagc                  <  1:768850/150‑1 (MQ=255)
                                                                                                                                   gCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGCGGGCAGAATAGTCGGTagcg                 >  1:408881/1‑150 (MQ=255)
                                                                                                                                    cagcatatGCGCAACATTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGAGATCCTGGTTCAGCTCTTCTTCCAGTAAGGCGGGCAGAATAGTCGGTagcgc                >  1:1063713/8‑150 (MQ=255)
                                                                                                                                     agcatatGCGCAACATTGCGCATATGCTGTTGGTGAATCACTGCTACTGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATTCAGTTCAGCGGTATCCTGGATCCGCTCTTCTTCCAGTAAGGCGGGCAGAATAGTCGGTagcgca               >  1:253328/7‑150 (MQ=255)
                                                                                                                                       catatGCGCAACATTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGCGGGCAGAATAGTCGGTagcgcagc             >  1:1526901/5‑150 (MQ=255)
                                                                                                                                        cACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAACTCTTCTTCCAGTAAGGCGGGCAGAATAGTCGGTagcgcagcg            <  1:1446092/150‑1 (MQ=255)
                                                                                                                                            gcacAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGGAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGCGGGCAGAATAGTCGGTAGCGCAGCGCGAt        >  1:1171363/4‑150 (MQ=255)
                                                                                                                                             cgcAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGCGGGCAGAATAGTCGGTAGCGCAGCGCGATg       <  1:1320232/150‑1 (MQ=255)
                                                                                                                                              gcAATGTTGTGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGCGGGCAGAATAGTCGGTAGCGCAGCGCGATgg      >  2:633393/1‑150 (MQ=255)
                                                                                                                                               cAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGCGGGCAGAATAGTCGGTAGCGCAGCGCGATGGa     <  1:735696/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
CGCCAGCCCGGCGCTCCATCCCCGGCATTCATCGATGCGGCAGATAACCCGTTGCGCATCGCAAGCCATAAACCCACAAGGAATGGACATCCAGTCCGGCGCGACGGTAGCGCGGGTGATGCCGTTTTCCTGCAACCACTGCGCAATGTTGCGCATATGCTGTTGGTGAATCACTGCTACGGTTGCCAGTTGCTGGTCGATATTCAACGGGGCGAAATGCAGTTCATCGATATCCTGGTTCAGCTCTTCTTCCAGTAAGGCGGGCAGAATAGTCGGTAGCGCAGCGCGATGGAAAT  >  NC_000913/3111815‑3112110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: