Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,114,519 G→A 6.1% intergenic (‑167/+31) pppA ← / ← sslE bifunctional prepilin leader peptidase/ methylase/putative secreted and surface‑associated lipoprotein mucinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,114,5190GA 6.1% 355.6 / 7.6 114intergenic (‑167/+31)pppA/sslEbifunctional prepilin leader peptidase/ methylase/putative secreted and surface‑associated lipoprotein mucinase
Reads supporting (aligned to +/- strand):  ref base G (55/51);  new base A (3/4);  total (58/56)
Fisher's exact test for biased strand distribution p-value = 7.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 119 total aligned reads displayed.

TGTAACTTGCTGGATGCGGCGTAAAACGCCTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGA  >  NC_000913/3114370‑3114668
                                                                                                                                                     |                                                                                                                                                     
tgtAACTTGCTGGATGCGGCGTAAAACGCCTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATg                                                                                                                                                       >  2:1080549/1‑150 (MQ=255)
  tAACTTGCTGGATGCGGCGTAAAACGCCTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATgcg                                                                                                                                                     >  1:134696/1‑150 (MQ=255)
   aaCTTGCTGGCTGCGGCGTAAAACGCCTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATgcgg                                                                                                                                                    <  1:715555/150‑1 (MQ=255)
   aaCTTGCTGGATGCGGCGTAAAACGCCTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATgcgg                                                                                                                                                    >  1:1064052/1‑150 (MQ=255)
       tGCTGGATGCGGCGTAAAACGCCTTATCCGTCCTACGGGTGTCTGCGGGCGCAATTAATAGCGGCTTTCCCCGTGTGTTGGGGAGATTAGCAGGAGCATCGGGCAAATGTGTTCACCCGGTGCGCCTTATTTCATGCCGGATGCGGcgcg                                                                                                                                                >  1:324461/1‑150 (MQ=255)
        gCTGGATGCGGCGTAAAACGCCTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGa                                                                                                                                               >  1:1529487/1‑150 (MQ=255)
         cTGGATGCGGCGTAAAACGCCTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAg                                                                                                                                              <  1:749103/150‑1 (MQ=255)
         cTGGATGCGGCGTAAAACGCCTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTCTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCAAGCCGGATGCGGCGCGAg                                                                                                                                              <  2:86317/150‑1 (MQ=255)
            gATGCGGCGTAAAACGCCTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAgcgc                                                                                                                                           >  2:886341/1‑150 (MQ=255)
               gcggcgTAAAACGCCTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCtt                                                                                                                                        <  1:507948/150‑1 (MQ=255)
                cggcgTAAAACGCCTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTa                                                                                                                                       >  2:108183/1‑150 (MQ=255)
                cggcgTAAAACGCCTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTa                                                                                                                                       >  2:76210/1‑150 (MQ=255)
                cggcgTAAAACGCCTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCACGAGCGCCTTa                                                                                                                                       <  2:756329/150‑1 (MQ=255)
                 ggcgTAAAACGCCTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTAt                                                                                                                                      >  2:1577023/1‑150 (MQ=255)
                  gcgTAAAACGCCTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATc                                                                                                                                     >  2:47116/1‑150 (MQ=255)
                   cgTAAAACGCCTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGAAGGACCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATcc                                                                                                                                    >  1:67978/1‑150 (MQ=255)
                   cgTAAAACGCCTTATCCGTCCTACGGGTGTCTGCCACCGCAAATAATAGCTGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGACTTATcc                                                                                                                                    >  2:139008/1‑150 (MQ=255)
                    gTAAAACGCCTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCg                                                                                                                                   >  2:178732/1‑150 (MQ=255)
                     tAAAACGCCTTATCCTTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATAAGGCGCTCGCGCCGCATCCgg                                                                                                                                  <  1:340931/150‑1 (MQ=255)
                     tAAAACGCCTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCgg                                                                                                                                  <  1:1199512/150‑1 (MQ=255)
                       aaaCGCCTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGcc                                                                                                                                <  2:883383/150‑1 (MQ=255)
                       aaaCGCCTTATCCGTCCTACGGGTGTCTGCCAGAGCAAATAATCGCTGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCATATGTGTTAACCCGGTGCGCCTTATTTCATACCGGATGCGGCGAGAGCGCATTATACGGcc                                                                                                                                >  1:1113264/1‑150 (MQ=255)
                         ccGCCTTCTCCGTCCTCCGGGTGTCTGCCCGCGCAAATCATCGCGGCTCTCCCCGTCTGTCGGCCCGATCAGCCGGCGCAGCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATAAGGCGCTCGCGCCGCATCCGGcatg                                                                                                                              <  1:324600/149‑4 (MQ=255)
                           gCCTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACg                                                                                                                            <  2:1399258/150‑1 (MQ=255)
                             cTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACggg                                                                                                                          >  1:1377435/1‑150 (MQ=255)
                             cTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACggg                                                                                                                          <  2:1529487/150‑1 (MQ=255)
                              ttATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGc                                                                                                                         <  2:1223175/150‑1 (MQ=255)
                               tATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCt                                                                                                                        <  1:1009072/150‑1 (MQ=255)
                                   cGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACt                                                                                                                    >  2:629383/1‑150 (MQ=255)
                                   cGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACt                                                                                                                    >  1:552948/1‑150 (MQ=255)
                                       cTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGc                                                                                                                <  1:163379/150‑1 (MQ=255)
                                         aCGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAg                                                                                                              <  1:1447900/150‑1 (MQ=255)
                                          cGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCTGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGa                                                                                                             <  2:707686/150‑1 (MQ=255)
                                            ggTGTCTGCCAGCGCAAATAATCTCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTCTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACa                                                                                                           <  2:40343/150‑1 (MQ=255)
                                            ggTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACa                                                                                                           >  2:1452260/1‑150 (MQ=255)
                                                 cTGCCAGCGCAAATAATCGCGGCGTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTa                                                                                                      <  2:1339376/150‑1 (MQ=255)
                                                     cAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACTGGCTTACTCGGCAGACATCTTATGCt                                                                                                  >  2:1365337/1‑150 (MQ=255)
                                                      agggcAAATAATCGCTGCTTTCCCCTTCTGTAGTCCCGATAAGCAGGCGCATCTGTCAAATGTTTTAACCCGGTGCTCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGATc                                                                                                 <  2:1113264/147‑1 (MQ=38)
                                                      aGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTc                                                                                                 >  2:1027518/1‑150 (MQ=255)
                                                        cgcAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCgg                                                                                               <  2:134696/150‑1 (MQ=255)
                                                        cgcAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCgg                                                                                               >  2:936612/1‑150 (MQ=255)
                                                        cgcAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCgg                                                                                               <  2:1708778/150‑1 (MQ=255)
                                                         gcAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGt                                                                                              <  2:539889/150‑1 (MQ=255)
                                                              taatCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCt                                                                                         <  1:1320496/150‑1 (MQ=255)
                                                                 tCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGAt                                                                                      <  1:1599052/150‑1 (MQ=255)
                                                                   gcgGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTa                                                                                    >  2:1352406/1‑150 (MQ=255)
                                                                    cgGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACTGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTaa                                                                                   <  1:76210/150‑1 (MQ=255)
                                                                    cgGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTaa                                                                                   <  1:108183/150‑1 (MQ=255)
                                                                       cTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATgg                                                                                <  1:327832/150‑1 (MQ=255)
                                                                       cTTTCCCCGTCTGTAAGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATgg                                                                                >  2:89948/1‑150 (MQ=255)
                                                                            cccGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTcc                                                                           >  1:1354432/1‑150 (MQ=255)
                                                                            cccGGCTGTCGGCCCGCTAAGCAGGCGCATAGGTCAAATGTGTTAACCCGGGGCGACTTTTTTCATGCGGGTTGGGGGGATAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTcc                                                                           <  2:324461/150‑1 (MQ=255)
                                                                              cGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCgg                                                                         >  2:1091485/1‑150 (MQ=255)
                                                                                     aGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTcc                                                                      <  1:1183065/146‑1 (MQ=255)
                                                                                     aGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTcc                                                                      >  2:1183065/1‑146 (MQ=255)
                                                                                     aGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGt                                                                  <  1:178732/150‑1 (MQ=255)
                                                                                       gCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGACTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGTCAGACATCTTATGCTCGGTACGCGGATTAATGGTTTCCGGTCCCTGtta                                                                >  2:314777/1‑149 (MQ=39)
                                                                                          cGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCgg                                                             >  2:128923/1‑150 (MQ=255)
                                                                                           gATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCTGGTCCGGtt                                                           >  1:162170/1‑150 (MQ=255)
                                                                                               aGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCg                                                        >  1:599206/1‑150 (MQ=255)
                                                                                               aGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCg                                                        <  1:829112/150‑1 (MQ=255)
                                                                                               aGCAGGCGCATCGGGCAAATGGGGTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACGCGGCAGACATCTTATGCTCGGTAACGTGAGTAATGGTTTCCGGTCCCTGTTCCGGTTTCg                                                        <  2:911881/150‑1 (MQ=39)
                                                                                                 cAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGc                                                      <  1:1657916/150‑1 (MQ=255)
                                                                                                   ggCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAg                                                    >  2:673430/1‑150 (MQ=255)
                                                                                                    gcgcATCGGGCAAATGTGTTAACCGGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGa                                                   <  2:1380385/150‑1 (MQ=255)
                                                                                                         tCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAg                                              >  1:1694667/1‑150 (MQ=255)
                                                                                                          cGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGACTTATCCGGCCTTCGGGCTTACTCGGCAGACCTCTTATGCTCGGGAACCTGATTAGTGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAgg                                             >  2:82234/1‑149 (MQ=255)
                                                                                                           gggCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACCGCTTCTGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTg                                            >  1:1530931/1‑150 (MQ=255)
                                                                                                            ggCAAATGTGTTTACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGa                                           <  1:87704/150‑1 (MQ=255)
                                                                                                            ggCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGa                                           >  1:1219965/1‑150 (MQ=255)
                                                                                                            ggCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGa                                           >  1:980571/1‑150 (MQ=255)
                                                                                                              caaagccgtTACCCCGGCGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGAcg                                         <  2:1569173/143‑1 (MQ=255)
                                                                                                                aaTGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGAcgcg                                       >  1:326375/1‑150 (MQ=255)
                                                                                                                aaTGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGAcgcg                                       >  1:782973/1‑150 (MQ=255)
                                                                                                                  tgtgTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAg                                     >  2:1243477/1‑150 (MQ=255)
                                                                                                                    tgtTAACCCGGTGCGCCTTATTTCATGCCGGATTCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCg                                   <  2:235592/150‑1 (MQ=255)
                                                                                                                    tgtTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCg                                   <  1:1577023/150‑1 (MQ=255)
                                                                                                                       taccgcGGGGCGGCTTATTTCATGCCGGATGCGGCGGTAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCgtgt                                <  1:139008/145‑1 (MQ=255)
                                                                                                                       tAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTTCTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGATTGACGCGAGCgtgt                                >  2:1058548/1‑150 (MQ=255)
                                                                                                                         aCCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGtgttg                              <  1:1027518/150‑1 (MQ=255)
                                                                                                                          cccGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGtgttgt                             <  2:38663/150‑1 (MQ=255)
                                                                                                                           ccGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTa                            <  2:552948/150‑1 (MQ=255)
                                                                                                                               ggcgcCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGcc                        <  1:936612/149‑1 (MQ=255)
                                                                                                                                 cgcCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGAGTGTTGTAAGCCgc                      >  2:1266296/1‑149 (MQ=255)
                                                                                                                                  gcCTTATTTCATGCCGGATGCGGCGCCAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTAACGCGAGCGTGTTGTAAGCCGAc                     >  1:1137216/1‑150 (MQ=255)
                                                                                                                                   gcTTATTTCATTCCGGATGCGGCGCGAGCGCCTTATCCGGCCGACGGGCTTTCTGGGCAGACATGTTATGGTGGGTTACCTGATTATTGGTTTGCGGTGCCTGTTGCGGGTTCGGGAGATCGAGTGACGCGCGCGTGTTGTAAGCCGGCt                    >  2:373952/2‑150 (MQ=255)
                                                                                                                                   ccTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGCCt                    >  2:1465935/1‑150 (MQ=255)
                                                                                                                                   ccTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACt                    >  2:51699/1‑150 (MQ=255)
                                                                                                                                      tATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGc                 <  2:1694667/150‑1 (MQ=255)
                                                                                                                                      tATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGc                 <  2:1404817/150‑1 (MQ=255)
                                                                                                                                       cccgtaggCCGGATAAGGCGCTCGCGCCGCATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCt                >  2:1248404/8‑150 (MQ=38)
                                                                                                                                        tttCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTc               <  1:471532/150‑1 (MQ=255)
                                                                                                                                          tCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCGGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTcac             >  2:1667367/1‑150 (MQ=255)
                                                                                                                                          tCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTcac             >  1:499865/1‑150 (MQ=255)
                                                                                                                                            aTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCacac           <  1:673430/150‑1 (MQ=255)
                                                                                                                                             ggCCGGATAAGGCGCTCGCGCCGCATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGTAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCacac           >  2:144200/2‑149 (MQ=255)
                                                                                                                                             ggCCGGATAAGGCGCTCGCGCCGCATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGTAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCacac           <  1:144200/148‑1 (MQ=255)
                                                                                                                                               ccGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGc        >  1:1704997/1‑150 (MQ=255)
                                                                                                                                               ccGGATAAGGCGCTCGCGCCGCATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGc        >  1:1658536/1‑150 (MQ=255)
                                                                                                                                                    ggcggggGGAGCGCGTTATCCGGCTTAGGGGCTACCCGGGCCGACATCTTATCCTCGGAACCCTGATAAATGGTTTCGGGTCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGa  <  2:162170/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGTAACTTGCTGGATGCGGCGTAAAACGCCTTATCCGTCCTACGGGTGTCTGCCAGCGCAAATAATCGCGGCTTTCCCCGTCTGTAGGCCCGATAAGCAGGCGCATCGGGCAAATGTGTTAACCCGGTGCGCCTTATTTCATGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGGCTTACTCGGCAGACATCTTATGCTCGGTAACCTGATTAATGGTTTCCGGTCCCTGTTCCGGTTTCGGCAGATCGAGTGACGCGAGCGTGTTGTAAGCCGACTGGCTCACACCGCCCTCGA  >  NC_000913/3114370‑3114668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: