BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	312,304	A→T	8.7%	I65L (ATA→TTA) ‡	ykgL →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	312,304	0	A	T	8.7%	319.4 / 15.0	124	I65L (ATA→TTA) ‡	ykgL	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base A (57/56); new base T (6/5); total (63/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.53e-01

Only 100 of 132 total aligned reads displayed.

AGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCGCT  >  NC_000913/312155‑312453

  tCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTtata                                                                                                                                                     <  2:1507375/150‑1 (MQ=255)

  tCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTtata                                                                                                                                                     <  1:1342517/150‑1 (MQ=255)

     aaaaGATGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAATATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAac                                                                                                                                                  <  1:943075/150‑1 (MQ=255)

     aaaaGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAac                                                                                                                                                  >  2:1138908/1‑150 (MQ=255)

     aaaaGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAac                                                                                                                                                  >  1:1175183/1‑150 (MQ=255)

       aaGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAacac                                                                                                                                                >  1:1672884/1‑150 (MQ=255)

       aaGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGAAGTTGGCCTCAAAAGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTTTAAacac                                                                                                                                                >  1:90299/1‑150 (MQ=255)

        agagGTTTGATGCCAGCATACAGGGTCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAacaca                                                                                                                                               <  1:301737/150‑1 (MQ=255)

            ggTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCaa                                                                                                                                           >  1:682682/1‑150 (MQ=255)

                gATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTGTTTATAACACCAACAtt                                                                                                                                       <  1:1595319/150‑1 (MQ=255)

                    ccAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATc                                                                                                                                   <  1:393379/150‑1 (MQ=255)

                     cAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATcc                                                                                                                                  >  1:1476205/1‑150 (MQ=255)

                         aTACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATc                                                                                                                              <  2:1247471/150‑1 (MQ=255)

                         aTACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTGTTTATAACACCAACATTAAttttcct                                                                                                                              <  2:29205/150‑7 (MQ=38)

                          tACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCt                                                                                                                             <  2:1672884/150‑1 (MQ=255)

                            cAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGa                                                                                                                           <  1:980490/150‑1 (MQ=255)

                            cAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGa                                                                                                                           >  1:501650/1‑150 (MQ=255)

                            cAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGa                                                                                                                           >  1:780277/1‑150 (MQ=255)

                             aGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGat                                                                                                                          >  1:1437311/1‑150 (MQ=255)

                                 gCGAATCTGGTCAACAAGTCAACATTAATATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAAAAGGAACAAAATTTACCCAGGAAAATTAATTTTCGTGTTATAAACACACAAACATTAATCAGATCAGattata                                                                                                                      >  2:766408/1‑149 (MQ=255)

                                 gCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGattatt                                                                                                                      <  2:9492/150‑1 (MQ=255)

                                  cGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGAttatta                                                                                                                     <  1:658061/150‑1 (MQ=255)

                                  cGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGAttatta                                                                                                                     >  2:178680/1‑150 (MQ=255)

                                       cTGGTCAACAAGTCAAGATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAg                                                                                                                <  2:318028/150‑1 (MQ=255)

                                       cTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAg                                                                                                                >  1:1404434/1‑150 (MQ=255)

                                       cTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATACCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAg                                                                                                                >  1:953906/1‑150 (MQ=255)

                                         ggTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGAc                                                                                                              <  2:1558550/150‑1 (MQ=255)

                                          gTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACa                                                                                                             <  1:1674629/150‑1 (MQ=255)

                                                   tCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGtt                                                                                                    <  1:1439016/150‑1 (MQ=255)

                                                   tCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCAATCTGATTATTAATCAGACAAATATCGtt                                                                                                    <  2:144052/150‑1 (MQ=255)

                                                       cATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCTGACTAATATCGTTAATc                                                                                                >  2:1356555/1‑150 (MQ=255)

                                                       aaTTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTTGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATc                                                                                                <  1:1494852/149‑1 (MQ=255)

                                                         ttAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCg                                                                                              <  2:981866/150‑1 (MQ=255)

                                                         ttAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATAAGACAAATATCGTTAATCCg                                                                                              >  1:291274/1‑150 (MQ=255)

                                                          tAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGt                                                                                             <  1:1104747/150‑1 (MQ=255)

                                                          tAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGt                                                                                             <  1:972081/150‑1 (MQ=255)

                                                           aaGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTc                                                                                            <  2:572873/150‑1 (MQ=255)

                                                            aGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCg                                                                                           <  2:1306162/150‑1 (MQ=255)

                                                                  tGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAAtttt                                                                                     >  2:639576/1‑150 (MQ=255)

                                                                    gAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGa                                                                                   >  2:383393/1‑150 (MQ=255)

                                                                      atatTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAAt                                                                                 <  1:533602/150‑1 (MQ=255)

                                                                           cGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACg                                                                            >  2:659278/1‑150 (MQ=255)

                                                                            ggAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGt                                                                           >  2:827426/1‑150 (MQ=255)

                                                                             gagagaAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAACCACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTATTCCGTCGATTTTTTATTCAACGtt                                                                          >  2:490127/1‑150 (MQ=255)

                                                                             gagagaAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATAAATCCGATCTGATTATTAATCAGACAAAAATCGTTAATCCTTCGAATTTTTAATCAACGtt                                                                          >  2:1114751/1‑150 (MQ=255)

                                                                              agagaAACGTCAGGCAGTTTGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAATCACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGttt                                                                         <  1:1088973/150‑1 (MQ=255)

                                                                              agagaAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGttt                                                                         <  2:1437311/150‑1 (MQ=255)

                                                                               gagaAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGtttt                                                                        >  1:262379/1‑150 (MQ=255)

                                                                               gagaAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGtttt                                                                        <  2:26794/150‑1 (MQ=255)

                                                                                agaAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGttttt                                                                       <  2:1175183/150‑1 (MQ=255)

                                                                                 gaAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGtttttt                                                                      >  1:916417/1‑150 (MQ=255)

                                                                                 gaAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGtttttt                                                                      >  2:58847/1‑150 (MQ=255)

                                                                                   aaCGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCt                                                                    <  2:529451/150‑1 (MQ=255)

                                                                                      gTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCACAGGAAAATTAATGTTGGTGTAATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTATTAc                                                                 >  1:537602/1‑150 (MQ=255)

                                                                                        cAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGa                                                               >  2:431992/1‑150 (MQ=255)

                                                                                            cAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCt                                                           <  2:80948/150‑1 (MQ=255)

                                                                                              gTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGa                                                         >  2:49501/1‑150 (MQ=255)

                                                                                                tGGCCTCAAATTAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAAc                                                       <  2:211899/150‑1 (MQ=255)

                                                                                                  gCCTGAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACgg                                                     <  1:328770/150‑1 (MQ=255)

                                                                                                  gCCTCAAAAGAAAACGAAGAATATAACCCCAGGAAAATTAATGTTGTAGTTATAAACACACCTACATAAATCCGTTCTGAATATAAATCAGTCAAATATCGTTAATCCGTCCAACTTAGAATCAACCTTTTTTCTTACGACCCTGAACgg                                                     <  1:333717/150‑1 (MQ=255)

                                                                                                    cTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGcc                                                   >  1:1397591/1‑150 (MQ=255)

                                                                                                    cTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACACTGAACGGca                                                   >  2:1204622/1‑149 (MQ=255)

                                                                                                      cAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCtt                                                 >  1:619700/1‑150 (MQ=255)

                                                                                                      cAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCtt                                                 <  2:682682/150‑1 (MQ=255)

                                                                                                       aaaTGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTa                                                >  1:997074/1‑150 (MQ=255)

                                                                                                           gAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAAc                                            <  2:501650/150‑1 (MQ=255)

                                                                                                              cAGGAAGAATATATCCCCATGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTg                                         <  2:537602/150‑1 (MQ=255)

                                                                                                              cAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTg                                         <  1:434587/150‑1 (MQ=255)

                                                                                                               aGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTgg                                        <  1:1043819/150‑1 (MQ=255)

                                                                                                               aGGAAGAATATATCCACAGGAAAATTAATGTTGGTCTTATAAACACACCAAAATTAATGCGATCTGATTATTAATCAGACAAATATCGTTTATCCGTCGAATTTTGATTCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTgg                                        <  1:1043820/150‑1 (MQ=255)

                                                                                                                ggAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGa                                       >  1:1454998/1‑150 (MQ=255)

                                                                                                                 gaagaaTATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCAGGCGAATATTGAATCAAAGTTTTTTCTTACGACCCTGAGCGGACTTAAAACGTGGAt                                      >  1:1173137/1‑150 (MQ=255)

                                                                                                                   agaaTATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTa                                    <  1:504421/150‑1 (MQ=255)

                                                                                                                   agaaTATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTa                                    >  1:876320/1‑150 (MQ=255)

                                                                                                                     aaTATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCAGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTATGAATCAACGTTTTTTCTTACTACCCTGAACGGCCTTAATACGTGGATTAGa                                  >  2:937922/1‑150 (MQ=255)

                                                                                                                      atatatCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGAt                                 <  1:371036/150‑1 (MQ=255)

                                                                                                                         tatCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTa                              >  2:970043/1‑150 (MQ=255)

                                                                                                                           tCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCTTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACa                            <  2:206325/150‑1 (MQ=255)

                                                                                                                           tCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACa                            <  2:953906/150‑1 (MQ=255)

                                                                                                                           tCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACa                            >  1:393805/1‑150 (MQ=255)

                                                                                                                             cccAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACaaa                          >  2:1193535/1‑150 (MQ=255)

                                                                                                                               cAGGAAAATTAATGTTGGTGATATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGACTCAACGTTTTTTCTTACGACCCAGAACGGCCTTAACCCGTTGATTAAATTTACAAAta                        >  1:932252/1‑149 (MQ=255)

                                                                                                                                aGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATca                       <  2:772803/150‑1 (MQ=255)

                                                                                                                                aGGAAAATTAATGTTGGTGGTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATca                       <  2:1432055/150‑1 (MQ=255)

                                                                                                                                 ggAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATcac                      >  1:1505478/1‑150 (MQ=255)

                                                                                                                                    ggaTTAATGTTGGTGTGTTTATAACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACata                   >  2:826660/3‑150 (MQ=39)

                                                                                                                                     aaTTAATGTTTGTGTTATAAACACAGCAACATTAATCCGATCTGATTATTAATGAGACAAATATCGTTAATACGTAGAATTTTGAATCTACGTTTTTTCTTACGACCCTGAACTGCCTTAAAACGTGGATTAGATTTACAAATCACatat                  <  2:1545062/150‑1 (MQ=255)

                                                                                                                                        tAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGc               <  1:679983/150‑1 (MQ=255)

                                                                                                                                          aTGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCg             <  1:748860/150‑1 (MQ=255)

                                                                                                                                          aTGTTGGTGTGTTTATAACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCg             >  1:656950/1‑150 (MQ=255)

                                                                                                                                          aTGTTGGTGTGTTTATAACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCg             >  1:877976/1‑150 (MQ=255)

                                                                                                                                           tGTTGGTGTGTTTATAACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACTGCCTTAAAAAGTGGATTATATTTACAAATCACATATAGCCGt            >  1:510310/1‑150 (MQ=255)

                                                                                                                                           tGTTGGTGTGTTTATAACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCgg            >  2:1075239/1‑149 (MQ=255)

                                                                                                                                            gTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTc           >  1:1382326/1‑150 (MQ=255)

                                                                                                                                                gtgtTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCttt       >  2:164663/1‑150 (MQ=255)

                                                                                                                                                 tgtTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAAAGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCtttt      >  1:46165/1‑150 (MQ=255)

                                                                                                                                                    tataAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCGc   >  1:529975/1‑150 (MQ=255)

                                                                                                                                                    tataAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCGc   >  2:917630/1‑150 (MQ=255)

                                                                                                                                                     ataAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCGCt  <  1:952741/150‑1 (MQ=255)

                                                                                                                                                     ataAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCGCt  <  1:970043/150‑1 (MQ=255)

AGTCTAAAAGAGGGTTGATGCCAGCATACAGGGGCGAAGCTGGTCAACAAGTCAACATTAAGATAATGGAATATTCGGAGAGAAACGTCAGGCAGTTGGCCTCAAATGAACAGGAAGAATATATCCCCAGGAAAATTAATGTTGGTGTTATAAACACACCAACATTAATCCGATCTGATTATTAATCAGACAAATATCGTTAATCCGTCGAATTTTGAATCAACGTTTTTTCTTACGACCCTGAACGGCCTTAAAACGTGGATTAGATTTACAAATCACATATAGCCGTCCTTTTCGCT  >  NC_000913/312155‑312453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`