Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,119,574 A→T 5.1% intergenic (‑462/+23) sslE ← / ← glcA putative secreted and surface‑associated lipoprotein mucinase/glycolate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,119,5740AT 5.1% 377.2 / 6.0 127intergenic (‑462/+23)sslE/glcAputative secreted and surface‑associated lipoprotein mucinase/glycolate transporter
Reads supporting (aligned to +/- strand):  ref base A (41/79);  new base T (3/4);  total (44/83)
Fisher's exact test for biased strand distribution p-value = 6.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 127 total aligned reads displayed.

TTGCGTGATAAAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCACGGTGTAGCGGAACAGTTCAGATTCTCGGCCCACCATGC  >  NC_000913/3119427‑3119721
                                                                                                                                                   |                                                                                                                                                   
ttGCGTGATAAAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAAc                                                                                                                                                   <  2:1193689/150‑1 (MQ=255)
 tgagTGATAAAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACg                                                                                                                                                  >  2:398496/4‑150 (MQ=255)
 tGCGTGATAAAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACg                                                                                                                                                  <  1:688673/150‑1 (MQ=255)
   cGTGATAAAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGat                                                                                                                                                >  2:757716/1‑150 (MQ=255)
   cGTGATAAAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGat                                                                                                                                                <  2:1460353/150‑1 (MQ=255)
     tGATAAAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGatca                                                                                                                                              >  2:1054830/1‑150 (MQ=255)
     tGATAAAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGatca                                                                                                                                              <  2:1495806/150‑1 (MQ=255)
          aaaaaaTATATGTTATCACGCTCTTAATTATTTAGCATGTTATCTGAACGCAAAACATTATTGCTGCATTGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCgg                                                                                                                                         <  1:860164/150‑1 (MQ=255)
          aaaaaaTAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATCGTTAATATCATCCgg                                                                                                                                         <  2:555318/150‑1 (MQ=255)
          aaaaaaTAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCgg                                                                                                                                         <  2:500315/150‑1 (MQ=255)
          aaaaaaTAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCgg                                                                                                                                         <  2:1430197/150‑1 (MQ=255)
           aaaaaTAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGc                                                                                                                                        <  2:1347441/150‑1 (MQ=255)
           aaaaaTAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGc                                                                                                                                        <  1:4007/150‑1 (MQ=255)
            aaaaTAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCt                                                                                                                                       >  1:603028/1‑150 (MQ=255)
             aaaTAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCtt                                                                                                                                      <  1:116946/150‑1 (MQ=255)
               aTAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGACATGATTTTCAATTAATAAGACAGAATAATGTAAAGGAAGCCGGATGATATTAACGATCATCCGGCTTTa                                                                                                                                    <  1:18424/150‑1 (MQ=255)
                  caTGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTg                                                                                                                                 <  1:1026703/149‑1 (MQ=255)
                   aTGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTTACGATCATCCGGCTTTATTGa                                                                                                                                >  1:1371320/1‑150 (MQ=255)
                    tGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGAt                                                                                                                               <  2:938620/150‑1 (MQ=255)
                    tGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGAt                                                                                                                               >  2:805764/1‑150 (MQ=255)
                        atcACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTAc                                                                                                                           <  1:94807/149‑1 (MQ=255)
                         tcACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACg                                                                                                                          >  1:674631/1‑150 (MQ=255)
                           aCGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACgag                                                                                                                        <  1:796778/150‑1 (MQ=255)
                           aCGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACgag                                                                                                                        >  1:1295395/1‑150 (MQ=255)
                           aCGCTATTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACgag                                                                                                                        <  2:240575/150‑1 (MQ=255)
                             gCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGAc                                                                                                                      <  1:286605/150‑1 (MQ=255)
                             gCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGAc                                                                                                                      <  2:827351/150‑1 (MQ=255)
                             gCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATAAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGAc                                                                                                                      <  2:309276/150‑1 (MQ=255)
                              ctctTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACt                                                                                                                     >  1:1349066/1‑150 (MQ=255)
                              ctctTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACt                                                                                                                     >  1:1164562/1‑150 (MQ=255)
                                ctTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTaa                                                                                                                   >  2:1161680/1‑150 (MQ=255)
                                  tAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACg                                                                                                                                                  <  1:407464/117‑1 (MQ=255)
                                  tAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACg                                                                                                                                                  >  2:407464/1‑117 (MQ=255)
                                  tAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACa                                                                                                                 >  1:886144/1‑150 (MQ=255)
                                    attattTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACTATCATCCGGCTTTATTGATTTACGAGACCAACATc                                                                                                               >  1:161445/1‑150 (MQ=255)
                                     ttattTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATcc                                                                                                              <  2:442211/150‑1 (MQ=255)
                                      tattTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATccc                                                                                                             <  1:927914/150‑1 (MQ=255)
                                      tattTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATccc                                                                                                             <  1:1599846/150‑1 (MQ=255)
                                       attTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCg                                                                                                            <  2:1231630/150‑1 (MQ=255)
                                       attTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCg                                                                                                            >  2:509495/1‑150 (MQ=255)
                                         ttAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGt                                                                                                          <  2:252099/150‑1 (MQ=255)
                                         ttAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGt                                                                                                          >  1:1166118/1‑150 (MQ=255)
                                          tAGCAGGGTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGCCTAACATCCCGGTa                                                                                                         <  1:889373/150‑1 (MQ=255)
                                           aGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTaa                                                                                                        >  1:833693/1‑150 (MQ=255)
                                            gCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTaaa                                                                                                       >  2:551203/1‑150 (MQ=255)
                                            gCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTaaa                                                                                                       >  2:888996/1‑150 (MQ=255)
                                             cAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAac                                                                                                      >  1:616974/1‑150 (MQ=255)
                                               ggTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAAATAATAAGACAGAATAATGTAAATGAAGCCGCATGATATTAACGATCATCCGGCTTAATTGATTTACGAGACTAACATCCCGGAAAacac                                                                                                    >  2:1708957/1‑150 (MQ=255)
                                                gTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAAcaca                                                                                                   <  2:140389/150‑1 (MQ=255)
                                                      tGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACCTACGcc                                                                                             >  1:717220/1‑150 (MQ=255)
                                                       gAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCt                                                                                            >  1:646431/1‑150 (MQ=255)
                                                          cGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTgca                                                                                         <  1:624150/150‑1 (MQ=255)
                                                          cGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTgca                                                                                         <  2:1166118/150‑1 (MQ=255)
                                                           gCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTgcag                                                                                        >  1:451183/1‑150 (MQ=255)
                                                            cAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTgcagc                                                                                       <  1:196347/150‑1 (MQ=255)
                                                                 cATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGt                                                                                  <  1:1297368/150‑1 (MQ=255)
                                                                 cATTATTGCTGCATGGATAATAAAGCGAGAAATCATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGt                                                                                  >  1:837801/1‑150 (MQ=255)
                                                                     attGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATa                                                                              >  2:505825/1‑150 (MQ=255)
                                                                      ttGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATaa                                                                             <  1:1054830/150‑1 (MQ=255)
                                                                       tgctgcATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCTGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAAt                                                                            >  1:283207/1‑150 (MQ=255)
                                                                        gctgcATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATg                                                                           <  1:805764/150‑1 (MQ=255)
                                                                         ctgcATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATATTGc                                                                          <  2:616974/150‑1 (MQ=255)
                                                                          tgcATGGATAATAAAGCGAGAAATGATTTTCAATTAATAATACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGcc                                                                         <  2:847595/150‑1 (MQ=255)
                                                                          tgcATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGcc                                                                         <  1:1697889/150‑1 (MQ=255)
                                                                          tgcATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGcc                                                                         <  1:389991/150‑1 (MQ=255)
                                                                           gcATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCg                                                                        <  1:1590382/150‑1 (MQ=255)
                                                                             aTGGATAATATAGCGAGAAATGATTTTCATTTAATAAGACAGAATAATGTAAATGAAGCCGGATGATTTTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATTCGCCTGCAGCAGGGTGATAATGCCGAt                                                                      <  2:41104/150‑1 (MQ=255)
                                                                              tGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATa                                                                     <  2:886144/150‑1 (MQ=255)
                                                                                   aataaAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCt                                                                <  2:192272/150‑1 (MQ=255)
                                                                                       aaGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCa                                                            <  1:551203/150‑1 (MQ=255)
                                                                                            agaAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAAt                                                       <  2:674631/150‑1 (MQ=255)
                                                                                             gaAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATc                                                      <  1:325181/150‑1 (MQ=255)
                                                                                             gaAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACAACCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATc                                                      <  1:757716/150‑1 (MQ=255)
                                                                                              aaaTGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCa                                                     <  2:540510/150‑1 (MQ=255)
                                                                                                   aTTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACtg                                                <  1:804082/150‑1 (MQ=255)
                                                                                                      ttCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGc                                             <  2:206175/150‑1 (MQ=255)
                                                                                                             aataaGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCACgg                                      <  2:1595839/150‑1 (MQ=255)
                                                                                                                aaGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCACGgtgt                                   <  2:283207/150‑1 (MQ=255)
                                                                                                                   aCAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCACGGTGTAGc                                <  2:1295395/150‑1 (MQ=255)
                                                                                                                     aGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCACGGTGTAGCgg                              <  2:833693/150‑1 (MQ=255)
                                                                                                                          aatGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCACGGTGTAGCGGAACAg                         <  1:885169/150‑1 (MQ=255)
                                                                                                                            tGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCACGGTGTAGCGGAACAGtt                       >  2:1615535/1‑150 (MQ=255)
                                                                                                                             tgaaaTGAAGCCGGACGATATTTACGATCATCCGGCTTTAATGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCACGGTGTAGCGGAACAGTTc                      <  2:1630096/148‑1 (MQ=255)
                                                                                                                              tAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCACGGTGTAGCGGAACAGTTCa                     <  1:509775/150‑1 (MQ=255)
                                                                                                                                aaTGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACACCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCACGGTGTAGCGGAACAGTTCAGa                   <  2:1349066/150‑1 (MQ=255)
                                                                                                                                  taaaGCCGGATGATCGTTAATATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCACGGTGTAGCGGAACAGTTCAGAtt                 >  1:1152073/3‑150 (MQ=255)
                                                                                                                                  tGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCACGGTGTAGCGGAACAGTTCAGAtt                 <  2:1118831/150‑1 (MQ=255)
                                                                                                                                     aGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCACGGTGTAGCGGAACAGTTCAGATtctc              >  1:67742/1‑150 (MQ=255)
                                                                                                                                     aGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCACGGTGTAGCGGAACAGTTCAGATtctc              <  2:1311268/150‑1 (MQ=255)
                                                                                                                                      gCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACAAACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTACACGGTGTAGCGGAACAGTTCAGATTCTCg             >  2:787575/1‑150 (MQ=255)
                                                                                                                                       ccGGATGATATTAACGATCATCCGGCTTTATTGATTTACGATACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGTTAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCACGGTGTAGCGGAACAGTTCAGATTCTCgg            <  1:1708957/150‑1 (MQ=255)
                                                                                                                                        cGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCACGGTGTAGCGGAACAGTTCAGATTCTCGGc           <  1:1615535/150‑1 (MQ=255)
                                                                                                                                          gatgatATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCACGGTGTAGCGGAACAGTTCAGATTCTCGGccc         <  1:706753/150‑1 (MQ=255)
                                                                                                                                           ttgatATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGGGATAATGCCGATAACGCTGGCAAATATCAGACTGTGCTTCACGGTGTAGCGGAACAGTTCAGATTCTCGGCcca        <  1:535219/149‑1 (MQ=255)
                                                                                                                                           atgatATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACCTCCCGGTAAACACATACGCCTGCAGCAGGGGGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCACGGTGTAGCGGAACAGTTCAGATTCTCGGCcca        <  1:509495/150‑1 (MQ=255)
                                                                                                                                            tgatATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCACGGTGTAGCGGAACAGTTCAGATTCTCGGCccac       <  2:1438533/150‑1 (MQ=255)
                                                                                                                                              atatTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCACGGTGTAGCGGAACAGTTCAGATTCTCGGCccacca     >  1:1080092/1‑150 (MQ=255)
                                                                                                                                              atatTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCACGGTGTAGCGGAACAGTTCAGATTCTCGGCccacca     >  1:611911/1‑150 (MQ=255)
                                                                                                                                              atatTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCACGGTGTAGCGGAACAGTTCAGATTCTCGGCccacca     >  2:1284603/1‑150 (MQ=255)
                                                                                                                                                atTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCACGGTGTAGCGGAACAGTTCAGATTCTCGGCCCACCATg   <  2:774100/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TTGCGTGATAAAAAAATAGATGTTCTCACGCTCTTAATTATTTAGCAGGTTATCTGAACGCAAAACATTATTGCTGCATGGATAATAAAGCGAGAAATGATTTTCAATTAATAAGACAGAATAATGTAAATGAAGCCGGATGATATTAACGATCATCCGGCTTTATTGATTTACGAGACTAACATCCCGGTAAACACATACGCCTGCAGCAGGGTGATAATGCCGATAACGCTGGCAAAAATCAGACTGTGCTTCACGGTGTAGCGGAACAGTTCAGATTCTCGGCCCACCATGC  >  NC_000913/3119427‑3119721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: