Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,136,628 T→G 6.0% intergenic (‑257/+35) pitB ← / ← gss phosphate transporter/glutathionylspermidine amidase and glutathionylspermidine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,136,6280TG 6.0% 514.8 / 17.8 166intergenic (‑257/+35)pitB/gssphosphate transporter/glutathionylspermidine amidase and glutathionylspermidine synthetase
Reads supporting (aligned to +/- strand):  ref base T (86/70);  new base G (2/8);  total (88/78)
Fisher's exact test for biased strand distribution p-value = 4.70e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

Only 100 of 183 total aligned reads displayed.

CTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACC  >  NC_000913/3136479‑3136777
                                                                                                                                                     |                                                                                                                                                     
cTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGattatt                                                                                                                                                       >  1:1133770/1‑150 (MQ=255)
   gtgtATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATTAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAAACCCCGTACTGATTAtactt                                                                                                                                                    >  2:107381/1‑150 (MQ=255)
    tgtATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTAttcttc                                                                                                                                                   >  2:672288/1‑150 (MQ=255)
    tgtATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTAttcttc                                                                                                                                                   <  1:1484065/150‑1 (MQ=255)
     gtATCAGACAATAAGAATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCACCGTACTGATTATTCTTCa                                                                                                                                                  >  1:842949/1‑150 (MQ=255)
       aTCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTATTAATa                                                                                                                                                >  1:1018414/1‑150 (MQ=255)
          aGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATc                                                                                                                                             >  1:751564/1‑150 (MQ=255)
          aGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATc                                                                                                                                             <  1:1290262/150‑1 (MQ=255)
             cAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTATTCATAATAAGt                                                                                                                                          >  2:197859/1‑150 (MQ=255)
              aaTAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTa                                                                                                                                         <  1:1347175/150‑1 (MQ=255)
              aaTAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTAATGATTATTCTTCATAATCAGTa                                                                                                                                         >  2:814461/1‑150 (MQ=255)
              aaTAAGCATTAATTCAAAACCTTTATAAATAATCAAAACACATGTATTACACTTGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCTTACTGATTATGAAGAATAATCAGTa                                                                                                                                         <  1:426211/150‑1 (MQ=255)
                 aaGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACgg                                                                                                                                      <  1:487673/150‑1 (MQ=255)
                  aGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATGAAGAATAATCAGTACggg                                                                                                                                     <  2:873979/150‑1 (MQ=255)
                   tcattcattCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATGAAGAATAATCAGTACgggg                                                                                                                                    <  1:309235/149‑1 (MQ=255)
                   gCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACgggg                                                                                                                                    <  2:1508768/150‑1 (MQ=255)
                       tcattcaAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGTGGTTGc                                                                                                                                >  2:179702/1‑150 (MQ=255)
                        cattcaAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCt                                                                                                                               >  1:869566/1‑150 (MQ=255)
                          ttcaAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTac                                                                                                                             >  2:41991/1‑150 (MQ=255)
                          ttcaAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATGAAGAATAATCAGTACggggattaatc                                                                                                                             <  1:601733/150‑8 (MQ=38)
                             aaaaCCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAAt                                                                                                                                              <  2:329064/130‑1 (MQ=255)
                             aaaaCCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAAt                                                                                                                                              >  1:329064/1‑130 (MQ=255)
                              aaaCCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGTGGTTGCTacaaca                                                                                                                         >  1:669773/1‑150 (MQ=255)
                              aaaCCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTacaaca                                                                                                                         >  1:1004720/1‑150 (MQ=255)
                                aCCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATg                                                                                                                       <  2:1386762/150‑1 (MQ=255)
                                   tttAGAAATAATCAAAACACAGGTATTCCACTGGTCAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTAt                                                                                                                    >  1:421080/1‑150 (MQ=255)
                                    ttAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTAtt                                                                                                                   <  2:1133770/150‑1 (MQ=255)
                                    ttAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTAtt                                                                                                                   <  1:856833/150‑1 (MQ=255)
                                     tAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTa                                                                                                                  >  2:349023/1‑150 (MQ=255)
                                      aGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAATACTGTATTTATACAACATGTATTAc                                                                                                                 >  1:100927/1‑150 (MQ=255)
                                           taatCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACttttt                                                                                                            <  1:271267/150‑1 (MQ=255)
                                           taatCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACttttt                                                                                                            <  2:857791/150‑1 (MQ=255)
                                            aatCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTc                                                                                                           <  2:1202052/150‑1 (MQ=255)
                                             atCAAAACACATGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTca                                                                                                          <  2:1701360/150‑1 (MQ=255)
                                             atCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTca                                                                                                          <  1:1561508/150‑1 (MQ=255)
                                             atCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTca                                                                                                          <  2:806073/150‑1 (MQ=255)
                                             atCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTca                                                                                                          <  1:387543/150‑1 (MQ=255)
                                             atCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTca                                                                                                          >  1:1226486/1‑150 (MQ=255)
                                                 aaaCACAGGTATTCCACTGGTTAATATATTCACATATGTAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTcaccac                                                                                                      >  1:616772/1‑150 (MQ=255)
                                                  aaCACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACcaca                                                                                                     <  2:1553864/150‑1 (MQ=255)
                                                  aaCACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACcaca                                                                                                     <  1:720380/150‑1 (MQ=255)
                                                    cacaGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAAt                                                                                                   >  1:536426/1‑150 (MQ=255)
                                                         gTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACg                                                                                              <  1:1492671/150‑1 (MQ=255)
                                                             tCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTc                                                                                          <  2:298659/150‑1 (MQ=255)
                                                              ccACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCa                                                                                         >  2:720744/1‑150 (MQ=255)
                                                              ccACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCa                                                                                         >  1:1676714/1‑150 (MQ=255)
                                                               cACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCaa                                                                                        >  1:1380844/1‑150 (MQ=255)
                                                                aCTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAat                                                                                       <  2:751564/150‑1 (MQ=255)
                                                                    gTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCa                                                                                   <  1:1552957/150‑1 (MQ=255)
                                                                     ttAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATTTCAc                                                                                  >  1:644619/1‑150 (MQ=255)
                                                                     ttAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCAc                                                                                  >  2:894307/1‑150 (MQ=255)
                                                                     ttAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCAc                                                                                  <  2:536426/150‑1 (MQ=255)
                                                                             ttCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCtttt                                                                          >  2:101654/1‑150 (MQ=255)
                                                                               cacaTATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCtttttt                                                                        >  2:1578608/1‑150 (MQ=255)
                                                                                 caTATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTtga                                                                      >  2:677460/1‑150 (MQ=255)
                                                                                 caTATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTtga                                                                      >  2:203778/1‑150 (MQ=255)
                                                                                  atatGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTtgat                                                                     >  1:965967/1‑150 (MQ=255)
                                                                                  atatGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTtgat                                                                     <  1:814461/150‑1 (MQ=255)
                                                                                   tatGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTtgatg                                                                    <  1:313718/150‑1 (MQ=255)
                                                                                   tatGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTtgatg                                                                    >  1:62078/1‑150 (MQ=255)
                                                                                        aatgaaTAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAg                                                               >  2:529531/1‑150 (MQ=255)
                                                                                         atgaaTAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGt                                                              <  1:738060/150‑1 (MQ=255)
                                                                                          tgaaTAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTg                                                             <  2:1380844/150‑1 (MQ=255)
                                                                                           gaaTAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGa                                                            >  2:810778/1‑150 (MQ=255)
                                                                                            aataaataTTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGAt                                                           <  1:237724/150‑1 (MQ=255)
                                                                                             ataaataTTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGAtt                                                          >  2:1685383/1‑150 (MQ=255)
                                                                                               aaataTTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATtca                                                        <  1:1589916/150‑1 (MQ=255)
                                                                                                  tatTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACAAGTGATtcatca                                                     >  1:1001619/1‑150 (MQ=255)
                                                                                                    ttGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCAcc                                                   >  1:979366/1‑150 (MQ=255)
                                                                                                     tGGAATATATAAATATTGAATATTTTGTTTAATACCAGTACTTATTATTCTTCATAATCAGTACGGGGTTGCTAAAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATTACCAGTGATTCATCACcg                                                  <  2:591108/150‑1 (MQ=255)
                                                                                                     tGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACcg                                                  >  2:1205978/1‑150 (MQ=255)
                                                                                                       gAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACcgcg                                                >  1:1537853/1‑150 (MQ=255)
                                                                                                       gAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACcgc                                                 <  1:1116876/149‑1 (MQ=255)
                                                                                                       gAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACcgc                                                 >  2:1116876/1‑149 (MQ=255)
                                                                                                       gAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAAATAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACcgcg                                                >  2:1219703/1‑150 (MQ=255)
                                                                                                        aaTATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCgtgc                                               >  2:1421109/1‑150 (MQ=255)
                                                                                                           atataAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCaaa                                            >  2:1468853/1‑150 (MQ=255)
                                                                                                           atataAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCaaa                                            <  1:1615651/150‑1 (MQ=255)
                                                                                                            tataAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAac                                           >  2:779916/1‑150 (MQ=255)
                                                                                                             ataAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAaca                                          <  2:1226486/150‑1 (MQ=255)
                                                                                                              taAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCAGAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAacac                                         >  2:230093/1‑150 (MQ=255)
                                                                                                                 atatTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACAACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTc                                      >  2:1324224/1‑150 (MQ=255)
                                                                                                                  tatTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTcc                                     >  1:1316378/1‑150 (MQ=255)
                                                                                                                   atTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTccc                                    >  1:634814/1‑150 (MQ=255)
                                                                                                                      gAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCAcc                                 >  2:571852/1‑150 (MQ=255)
                                                                                                                      gAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACAACAATTAACGGTTCAATATCACTCTCTTTTCTGATGACCAGTGATTCATCACCGCGAAAACACGTACcccc                                 >  1:877784/1‑150 (MQ=255)
                                                                                                                       aaTATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCg                                <  2:965967/150‑1 (MQ=255)
                                                                                                                        atatTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGt                               <  1:202519/150‑1 (MQ=255)
                                                                                                                                aTTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTtccg                       >  1:400600/1‑150 (MQ=255)
                                                                                                                                  tAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTgccgcc                     <  2:62078/150‑1 (MQ=255)
                                                                                                                                   aaTCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCa                    >  2:391909/1‑150 (MQ=255)
                                                                                                                                   aaTCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCa                    >  1:999450/1‑150 (MQ=255)
                                                                                                                                       cccGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACgg                <  2:1001619/150‑1 (MQ=255)
                                                                                                                                            aCTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAg           >  2:1254750/1‑150 (MQ=255)
                                                                                                                                             cTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAgg          <  1:1578608/150‑1 (MQ=255)
                                                                                                                                              tGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGt         >  1:1096970/1‑150 (MQ=255)
                                                                                                                                                 ttattCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACa      <  2:1658700/150‑1 (MQ=255)
                                                                                                                                                 ttattCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACa      >  1:959973/1‑150 (MQ=255)
                                                                                                                                                 ttattCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACa      <  1:1245495/150‑1 (MQ=255)
                                                                                                                                                  tattCTTCATAATCAGTACGGGGTTGCTACAAAATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTTATGACAAGTGATTAATCACCGCGCAAACACGTCCCACAGTAGTTGCCGCCAAAGGTGAAGGTACaa     >  1:172085/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAATATTGGAATATATAAATATTGAATATTTTGATTAATCCCCGTACTGATTATTCTTCATAATCAGTACGGGGTTGCTACAACATGTATTACTTTTTCACCACAATTAACGGTTCAATATCACTCTCTTTTTTGATGACCAGTGATTCATCACCGCGCAAACACGTCCCACCGTAGTTGCCGCCAACGGTGAAGGTACATACC  >  NC_000913/3136479‑3136777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: