Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,140,859 T→G 8.0% E143A (GAG→GCG)  hybD ← maturation protease for hydrogenase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,140,8590TG 8.0% 345.9 / 14.7 120E143A (GAG→GCG) hybDmaturation protease for hydrogenase 2
Reads supporting (aligned to +/- strand):  ref base T (57/52);  new base G (7/3);  total (64/56)
Fisher's exact test for biased strand distribution p-value = 3.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

ATCGTGCATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGC  >  NC_000913/3140710‑3141005
                                                                                                                                                     |                                                                                                                                                  
aTCGTGCATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCt                                                                                                                                                    >  1:1347797/1‑150 (MQ=255)
  cGTGCATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCa                                                                                                                                                  >  1:3623/1‑150 (MQ=255)
    tGCATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAg                                                                                                                                                <  2:914867/150‑1 (MQ=255)
    tGCATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAg                                                                                                                                                <  1:1597763/150‑1 (MQ=255)
    tGCATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAg                                                                                                                                                <  2:1079209/150‑1 (MQ=255)
      cATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAgcg                                                                                                                                              >  1:390371/1‑150 (MQ=255)
      cATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAgcg                                                                                                                                              >  1:510560/1‑150 (MQ=255)
       aTCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAgcgc                                                                                                                                             >  1:479833/1‑150 (MQ=255)
       aTCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAgcgc                                                                                                                                             >  2:353378/1‑150 (MQ=255)
       aTCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCGGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAgcgc                                                                                                                                             <  2:1122953/150‑1 (MQ=255)
        tCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCCCGCCAGATTCACGCAGCGCAGCCAGAACCTGCGCAAGCGCa                                                                                                                                            >  2:1089860/1‑150 (MQ=255)
        tCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCTGATTCACGCAGCGCAGCCAGAACCAGCTCAAGCGCa                                                                                                                                            >  1:297341/1‑150 (MQ=255)
        tCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCa                                                                                                                                            <  2:1287708/150‑1 (MQ=255)
         cGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCAAGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAg                                                                                                                                           <  2:976306/150‑1 (MQ=255)
          gAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAgg                                                                                                                                          <  1:543070/150‑1 (MQ=255)
          gAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAgg                                                                                                                                          <  1:1251974/150‑1 (MQ=255)
           aaCTCCGGGCATTTTCTTCAAACGCTGCGTTTAGATCCGGCTGCGGGGAGGGCTGGAACCCTGCTATCTCTTCATTCATGATTCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCTCAGCCAGAACCTGCTCCATCGCAGGt                                                                                                                                         <  2:667911/150‑1 (MQ=255)
             cGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTc                                                                                                                                       <  2:1153828/150‑1 (MQ=255)
               ccGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCAATCTCTTCAGTCATGACTCGCCTCCCGTTGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCGCTTGAGCAGGTTctg                                                                                                                                     <  1:1565538/150‑3 (MQ=255)
                cGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAAt                                                                                                                                    >  2:654577/1‑150 (MQ=255)
                cGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCGCTTGAGCAGGTTctgg                                                                                                                                    <  1:1174051/150‑4 (MQ=255)
                 tagaaaattcttcAAACGCTGAAAGTACTTGCGCCTTCGGGGAGGTAAGGAAAACTGATATCTCTTCAGTCATCAATCGCCTCCCTTGGTATGTCTACCAAGCCAGATTCACGCATCGCAGCCAGAACCTGCTCAAGCGCAGGTACAATc                                                                                                                                   >  2:198423/8‑150 (MQ=255)
                 gggCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATc                                                                                                                                   <  2:535453/150‑1 (MQ=255)
                    cAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTg                                                                                                                               <  2:795596/150‑1 (MQ=255)
                     aaTTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTg                                                                                                                               <  2:618545/150‑1 (MQ=255)
                          cttcAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTGAATCATTGCTTCa                                                                                                                          >  2:1414311/1‑150 (MQ=255)
                               aaCGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGt                                                                                                                     >  1:1081925/1‑150 (MQ=255)
                               aaCGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGt                                                                                                                     >  1:935019/1‑150 (MQ=255)
                               aaCGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGt                                                                                                                     >  2:786137/1‑150 (MQ=255)
                               aaCGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTAAACCgg                                                                                                                     >  1:1051766/1‑149 (MQ=255)
                                 cGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCg                                                                                                                   <  2:540796/150‑1 (MQ=255)
                                  gCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCgg                                                                                                                  <  1:1691331/150‑1 (MQ=255)
                                   cTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGc                                                                                                                 <  2:1477067/150‑1 (MQ=255)
                                          tACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGc                                                                                                          >  2:74298/1‑150 (MQ=255)
                                            cTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCg                                                                                                        >  1:799605/1‑150 (MQ=255)
                                                cgcCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGAtgt                                                                                                    >  2:746543/1‑150 (MQ=255)
                                                 gcCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCATCGGCTTAACGCCGacggt                                                                                                   <  2:430595/150‑5 (MQ=255)
                                                      cGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCt                                                                                              <  1:423679/150‑1 (MQ=255)
                                                        gggAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTcc                                                                                            >  2:403687/1‑150 (MQ=255)
                                                           aGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGc                                                                                         <  1:654577/150‑1 (MQ=255)
                                                                  gAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGAGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCg                                                                                  >  1:991208/1‑150 (MQ=255)
                                                                    aaCCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCggg                                                                                <  1:7260/150‑1 (MQ=255)
                                                                     aCCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGa                                                                               <  1:800966/150‑1 (MQ=255)
                                                                        tGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTg                                                                                                                               <  2:543582/99‑1 (MQ=255)
                                                                        tGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCa                                                                            <  2:420303/150‑1 (MQ=255)
                                                                        tGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCa                                                                            >  1:420303/1‑150 (MQ=255)
                                                                          cTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACg                                                                          >  2:1653345/1‑150 (MQ=255)
                                                                           tATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGc                                                                         <  1:1632268/150‑1 (MQ=255)
                                                                           tATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGc                                                                         >  1:657540/1‑150 (MQ=255)
                                                                            aTCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCATGCCAGATTCACGCAGCGCAGCCAGAACCTGCACAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGcc                                                                        <  1:696352/150‑1 (MQ=255)
                                                                            aTCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGcc                                                                        <  2:1081925/150‑1 (MQ=255)
                                                                            aTCTCTTCAGTAATGAATCGCCTCCCGTGGGATGGCTACCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGcc                                                                        <  2:137493/150‑1 (MQ=255)
                                                                              ctctTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGa                                                                      <  1:570092/150‑1 (MQ=255)
                                                                                 ttCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCATCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCa                                                                   <  2:1512976/150‑1 (MQ=255)
                                                                                  tCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCACAACCTGCTCCCGCGCCGGTTCAATCATAGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGTGATCACGCCGACCAg                                                                  <  2:264096/150‑1 (MQ=255)
                                                                                    cgTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAggg                                                                <  1:369206/149‑1 (MQ=255)
                                                                                    aGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAggg                                                                >  2:566392/1‑150 (MQ=255)
                                                                                     gTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGt                                                               >  2:682704/1‑150 (MQ=255)
                                                                                       cATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTcc                                                             >  2:987314/1‑149 (MQ=255)
                                                                                       cATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGAGTCACGCAGCGCAGCCAGAACCTGCGCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGGTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCa                                                             >  1:836175/1‑150 (MQ=255)
                                                                                          gAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTGCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCt                                                          <  1:58758/150‑1 (MQ=255)
                                                                                               gCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTc                                                     >  1:1258938/1‑150 (MQ=255)
                                                                                                ccTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCg                                                    <  2:390371/150‑1 (MQ=255)
                                                                                                ccTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCg                                                    <  1:74298/150‑1 (MQ=255)
                                                                                                 cTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGAGACCGGGATCACGCCGACCAGGGTCAGCTTTTTCgg                                                   >  1:1540067/1‑150 (MQ=255)
                                                                                                   cccGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCCAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAACCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGCCCAGGGTCAGCTTTTTCGGaa                                                 >  1:1286473/1‑150 (MQ=255)
                                                                                                   cccGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGaa                                                 >  1:582383/1‑150 (MQ=255)
                                                                                                     cGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGAGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAAc                                               <  2:158210/150‑1 (MQ=255)
                                                                                                     cGTGGGATGGCTTCCACACCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTTCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAAc                                               >  1:1170823/1‑150 (MQ=255)
                                                                                                              gCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTCAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTg                                      >  2:358553/1‑150 (MQ=255)
                                                                                                              gCTTCCACGCCAGACTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGCCCAGGGTCAGCTTTTTCGGAAACTCGCCGGTg                                      >  1:1604613/1‑150 (MQ=255)
                                                                                                               cTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGa                                     >  2:905940/1‑150 (MQ=255)
                                                                                                                 gccACGCCAGATGCACGCAGCGCAGCCAGTACCTGCTCAAGCGCAGGTTCAATCTTTGCTTCTACCGTTGGCGTTATGCCGATGTGTGGCTCCAGCGATTCGGGGATCACGCCGACCAGGGTCAGCTTTTTCTGAAACTCGCCGGTGAAg                                   <  1:79372/149‑1 (MQ=255)
                                                                                                                  ccACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCGTTTTCGGAAACTCGCCGGTGAAgc                                  <  2:510560/150‑1 (MQ=255)
                                                                                                                    aCGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAgcgc                                <  2:1258938/150‑1 (MQ=255)
                                                                                                                     cGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCa                               <  2:1557129/150‑1 (MQ=255)
                                                                                                                     cGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCa                               >  1:1389650/1‑150 (MQ=255)
                                                                                                                      gCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCACCCGTCGGCGTTACGCCGATGTGTGGCTCCAGCGATTCCGGGCTCACGCCGACCAGGGTCCGCTTTTTCGGAAACTCGCCGGTGAAGCGCAg                              >  1:74679/1‑150 (MQ=255)
                                                                                                                      gCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAg                              >  2:1119287/1‑150 (MQ=255)
                                                                                                                               aCGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACaa                     >  1:951394/1‑150 (MQ=255)
                                                                                                                               aCGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACaa                     >  1:266213/1‑150 (MQ=255)
                                                                                                                                cgcagcgcagCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAACCTCGCCGGTGAAGCGCAGGGCCGACAAg                    >  1:932087/1‑150 (MQ=255)
                                                                                                                                cgcagcgcagCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAg                    >  1:144734/1‑150 (MQ=255)
                                                                                                                                cgcagcgcagCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAg                    >  1:1500176/1‑150 (MQ=255)
                                                                                                                                    gcgcagcCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCACCCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGt                <  2:1559834/150‑1 (MQ=255)
                                                                                                                                       cagcCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCgg             >  1:1475366/1‑150 (MQ=255)
                                                                                                                                       cagcCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCgg             >  1:295169/1‑150 (MQ=255)
                                                                                                                                       cagcCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTCAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCgg             <  1:358553/150‑1 (MQ=255)
                                                                                                                                        agcCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggc            <  1:403687/150‑1 (MQ=255)
                                                                                                                                         gcCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGATACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggcc           >  1:1186083/1‑150 (MQ=255)
                                                                                                                                          ccAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggcca          >  1:797780/1‑150 (MQ=255)
                                                                                                                                          attgAACCTGCGCTTGAGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggcca          >  2:1479615/4‑150 (MQ=255)
                                                                                                                                            aGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTGCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggccagg        >  1:87555/1‑150 (MQ=255)
                                                                                                                                            aGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGGTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGGCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggccagg        <  1:92736/150‑1 (MQ=255)
                                                                                                                                             gAACCTGCTCAAGCGCAGGGTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggccaggc       <  1:705272/150‑1 (MQ=255)
                                                                                                                                             gAACCTGCGCTTGAGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggccaggc       >  2:590919/1‑150 (MQ=255)
                                                                                                                                             gAACCTGCGCTTGAGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggccaggc       >  1:1369049/1‑150 (MQ=255)
                                                                                                                                              aaCCTGCGCTTGAGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCggccaggcc      >  1:1406898/1‑150 (MQ=255)
                                                                                                                                                ccTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCaa    >  2:197688/1‑150 (MQ=255)
                                                                                                                                                  tGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGc  <  2:804981/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
ATCGTGCATCGAACGCCGGGCAATTTCTTCAAACGCTGCCTGTACTTGCGCCTTCGGGGAGGTCTGGAAACCTGCTATCTCTTCAGTCATGAATCGCCTCCCGTGGGATGGCTTCCACGCCAGATTCACGCAGCGCAGCCAGAACCTGCTCAAGCGCAGGTTCAATCATTGCTTCAACCGTCGGCGTTAAGCCGATGTGTGGCTCCAGCGATTCCGGGATCACGCCGACCAGGGTCAGCTTTTTCGGAAACTCGCCGGTGAAGCGCAGGGCCGACAAGACGTCGGCCAGGCCAAGC  >  NC_000913/3140710‑3141005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: