Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,151,957 G→T 6.6% L10I (CTT→ATT) ‡ exbB ← membrane spanning protein in TonB‑ExbB‑ExbD complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,151,9570GT 6.6% 357.6 / 6.1 118L10I (CTT→ATT) ‡exbBmembrane spanning protein in TonB‑ExbB‑ExbD complex
Reads supporting (aligned to +/- strand):  ref base G (62/47);  new base T (2/6);  total (64/54)
Fisher's exact test for biased strand distribution p-value = 1.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

GCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAACGACGTCGAATTGATAGTCGTTCTCATTACTATTTGCATACTGCCGTACCTTTGCTTTCTTTTCCTTG  >  NC_000913/3151810‑3152103
                                                                                                                                                    |                                                                                                                                                  
gctCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAATTAAGCCCATTCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAATGt                                                                                                                                                  >  2:1513188/1‑150 (MQ=255)
gctCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGt                                                                                                                                                  >  2:223888/1‑150 (MQ=255)
       cTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGACCTTTCCGTCTg                                                                                                                                           <  2:1482615/150‑1 (MQ=255)
       cTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTg                                                                                                                                           <  1:78951/150‑1 (MQ=255)
       cTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTg                                                                                                                                           >  2:1536019/1‑150 (MQ=255)
       cTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTg                                                                                                                                           <  1:119446/150‑1 (MQ=255)
       cTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTg                                                                                                                                           >  2:875291/1‑150 (MQ=255)
        ttAAGGCGTCGCTTCTGAGTGAAGAACTCTACGCTCTTACTGATGAAGCTTGCCCAGGTGACTTCGGCGGCCAAAATAAGCCGATTCATCACGGTCTTTACGACAATTTCGGCGCGCTGATAGATACCCCAGACGGACCTTTCCGTCTgg                                                                                                                                          <  1:311055/150‑2 (MQ=255)
        ttAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGACCTTTCCGTCTgg                                                                                                                                          <  1:1476601/150‑2 (MQ=255)
        ttAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGACCTTTCCGTCTgg                                                                                                                                          <  1:12712/150‑2 (MQ=255)
        ttAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGc                                                                                                                                          <  2:344448/150‑1 (MQ=255)
         tAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGACCATTCCGTCTggg                                                                                                                                         <  2:753565/150‑3 (MQ=255)
           aGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGACCTTTCCGTCTggggt                                                                                                                                       <  1:696365/150‑5 (MQ=255)
           aGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCAtt                                                                                                                                       >  1:1436843/1‑150 (MQ=255)
           aGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCAtt                                                                                                                                       >  1:342119/1‑150 (MQ=255)
            ggCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTa                                                                                                                                      >  2:1466837/1‑150 (MQ=255)
               gACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAat                                                                                                                                   >  2:1118231/1‑150 (MQ=255)
                   cTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAattatt                                                                                                                               >  1:124745/1‑150 (MQ=255)
                    ttCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAAttatta                                                                                                                              >  2:1662599/1‑150 (MQ=255)
                     tCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTAc                                                                                                                             <  1:839466/150‑1 (MQ=255)
                       tGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTAccc                                                                                                                           <  2:549911/150‑1 (MQ=255)
                            gaagccctctACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAATTAAGCCCATTCATCACGCACTTACCGACAATATCGGCGTGCTGATACATACCCCAGACGGAACGGTCCGTCTGCATTAATTTATTACCCCCtgg                                                                                                                      >  2:1243201/7‑149 (MQ=255)
                            gaagaaCTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACtgt                                                                                                                      >  2:390812/1‑150 (MQ=255)
                              agaaCTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTgtgt                                                                                                                    >  1:1604435/1‑150 (MQ=255)
                               gaaCTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTa                                                                                                                   <  1:195969/150‑1 (MQ=255)
                               gaaCTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTa                                                                                                                   <  2:1429123/150‑1 (MQ=255)
                                 aCTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTAtc                                                                                                                 >  1:1063108/1‑150 (MQ=255)
                                   tctACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGACCTTTCCGTCTgg                                                                                                                                          >  2:302465/1‑122 (MQ=255)
                                   tctACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGACCTTTCCGTCTgg                                                                                                                                          <  1:302465/123‑2 (MQ=255)
                                   tctACGCTATTACTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCGGTCTGCATTAAATTATTACCCACTGTGTAtctc                                                                                                               <  1:352833/150‑1 (MQ=255)
                                    ctACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTcc                                                                                                              >  2:1062951/1‑150 (MQ=255)
                                    ctACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTcc                                                                                                              >  2:556113/1‑150 (MQ=255)
                                         ctctTACTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGAc                                                                                                         >  2:1315555/1‑150 (MQ=255)
                                         ctctTACTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGAc                                                                                                         <  2:4373/150‑1 (MQ=255)
                                          tctTACTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACg                                                                                                        <  2:124745/150‑1 (MQ=255)
                                            ttACTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCa                                                                                                      <  1:225999/150‑1 (MQ=255)
                                             tACTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTTTTACCCACTGTGTATCTCCAGGACGCaa                                                                                                     >  1:1548381/1‑150 (MQ=255)
                                              aCTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAg                                                                                                    <  2:880181/150‑1 (MQ=255)
                                              aCTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAg                                                                                                    <  1:628280/150‑1 (MQ=255)
                                               cTGAAGTAGATTGCCCAGGTGACTACGGAGGCCATAATAAGCCGAATCATCACGCACTTAACGACTATATCGGGGTGCTGATACATTCGCCAGACGGAAAGGTCCGTCTGCATTAATTTATTACCCACTGTGTATCTCCTGGACGCAAGt                                                                                                   <  1:898141/150‑1 (MQ=255)
                                               cTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCATCGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAg                                                                                                    >  2:1450296/1‑150 (MQ=255)
                                                tGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTc                                                                                                  >  2:1491107/1‑150 (MQ=255)
                                                tGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTc                                                                                                  <  1:1256922/150‑1 (MQ=255)
                                                 gaagaaGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTca                                                                                                 >  2:609184/1‑150 (MQ=255)
                                                   agaagaTTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTcaca                                                                                               <  1:998971/150‑1 (MQ=255)
                                                    gaagaTTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACaa                                                                                              >  2:812561/1‑150 (MQ=255)
                                                              cAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCa                                                                                    >  1:1039263/1‑150 (MQ=255)
                                                                 gTGACTACGGAGGCCAAAATAAGCCCAATCATCCCGAACATAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCataa                                                                                 >  1:1089383/1‑150 (MQ=255)
                                                                      tACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCataataata                                                                            <  1:494105/150‑1 (MQ=255)
                                                                       aCGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAatat                                                                           >  1:756137/1‑150 (MQ=255)
                                                                        cGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATc                                                                          <  2:189849/150‑1 (MQ=255)
                                                                         ggAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCa                                                                         <  2:342119/150‑1 (MQ=255)
                                                                          gAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCaa                                                                        <  2:861314/150‑1 (MQ=255)
                                                                              ccAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAacg                                                                    >  1:836318/1‑150 (MQ=255)
                                                                              ccAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAacg                                                                    >  1:1419758/1‑150 (MQ=255)
                                                                              ccAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAacg                                                                    >  1:1250432/1‑150 (MQ=255)
                                                                               cAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAacga                                                                   >  2:876312/1‑150 (MQ=255)
                                                                                aaaaTAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAacgac                                                                  <  1:364380/150‑1 (MQ=255)
                                                                                    tAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTTAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAACGACGTCg                                                              >  1:1288379/1‑150 (MQ=255)
                                                                                    tAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAACGACGTCg                                                              <  1:875291/150‑1 (MQ=255)
                                                                                      aGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAACGACGTCGaa                                                            >  1:656645/1‑150 (MQ=255)
                                                                                       gCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAACGACGTCGAAt                                                           >  2:391195/1‑150 (MQ=255)
                                                                                        cccAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAACGACGTCGAAtt                                                          >  2:1296768/1‑150 (MQ=255)
                                                                                            atcatcACGCACTTAACGACAATATCGGCGTGCTGATACAGACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAACGACGTCGAATTGATa                                                      >  1:1602595/1‑150 (MQ=255)
                                                                                             tcttcaCGCACTTAACGACAATATCGGCGTGCTGATAGATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACGCACTGTGTATCTCCAGGACGCAAGTCTCAAAATCTGCGCATAATAATATCAAAACGACGTCGAATTGATAg                                                     <  2:1089383/147‑1 (MQ=255)
                                                                                             tcatcaCGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAACGACGTCGAATTGATAg                                                     >  2:138039/1‑150 (MQ=255)
                                                                                             tcatcaCGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAACGACGTCGAATTGATAg                                                     >  1:347342/1‑150 (MQ=255)
                                                                                                  aCGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAACGACGTCGAATTGATAGTCGtt                                                >  2:388874/1‑150 (MQ=255)
                                                                                                    cGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAACGACGTCGAATTGATAGTCGTtc                                               >  1:536657/1‑150 (MQ=255)
                                                                                                     gCACTTAACGACAATATCGGCGTGCTGATATATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAACGACGTCGAATTGATAGTCGTtct                                              >  2:1256504/1‑150 (MQ=255)
                                                                                                      cACTTAACGACAATATCGGCGTGCTGCTACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAACGACGTCGAATTGATAGTCGTtctc                                             >  2:503324/1‑150 (MQ=255)
                                                                                                      cACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAACGACGTCGAATTGATAGTCGTtctc                                             >  1:525157/1‑150 (MQ=255)
                                                                                                       aCTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAACGACGTCGAATTGATAGTCGTTCTCa                                            <  1:67051/150‑1 (MQ=255)
                                                                                                          tAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAACGACGTCGAATTGATAGTCGTTCTCATTa                                         >  1:1059654/1‑150 (MQ=255)
                                                                                                          tAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAACGACGTCGAATTGATAGTCGTTCTCATTa                                         >  1:724003/1‑150 (MQ=255)
                                                                                                             cGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAACGACGTCGAATTGATAGTCGTTCTCATTACTa                                      >  2:791886/1‑150 (MQ=255)
                                                                                                                 aaTATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAACGACGTCGAATTGATAGTCGTTCTCATTACTATTTg                                  >  2:1013108/1‑150 (MQ=255)
                                                                                                                 aaTATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAACGACGTCGAATTGATAGTCGTTCTCATTACTATTTg                                  <  2:787717/150‑1 (MQ=255)
                                                                                                                   tatCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAACGACGTCGAATTGATAGTCGTTCTCATTACTATTTGCa                                <  1:1536019/150‑1 (MQ=255)
                                                                                                                    atCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATATCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAACGACCACGAATTGATAGTCGATCTCATTACTATTTGCAt                               >  2:502323/1‑150 (MQ=255)
                                                                                                                      cGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATATTAATATCAAAACGACGTCGAATTGATAGTCGTTCTCATTACTATTTGCATAc                             <  1:1544960/150‑1 (MQ=255)
                                                                                                                      cGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAACGACTTCGAATTGATAGTCGTTCTCATTACTATTTGCATaa                             <  1:1466837/150‑2 (MQ=255)
                                                                                                                            gCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAACGACGTCGAATTGATAGTCGTTCTCATTACTATTTGCATACTGCCGt                       >  2:1415390/1‑150 (MQ=255)
                                                                                                                               gATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAACGACGTCGAATTGATAGTCGTTCTCATTACTATTTGCATACTGCCGTAcc                    <  2:1039263/150‑1 (MQ=255)
                                                                                                                                 tacatacCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGAAGCAAGTCACAAAATCTGCGCATAATAAAATCAAAACGACGTCGAATTGATAGTCGTTCTCATTACTATTTGCATACTGCCGTACCtt                  >  1:1357858/1‑150 (MQ=255)
                                                                                                                                  acatacCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAACGACGTCGAATTGATAGTCGTTCTCATTACTATTTGCATACTGCCGTACCttt                 <  1:1450296/150‑1 (MQ=255)
                                                                                                                                  acatacCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCAAAATAATATCAAAACGACGTCGAATTGATAGTCGTTCTCATTACTATTTGCATACTGCCGTACCttt                 >  2:1227120/1‑150 (MQ=255)
                                                                                                                                   catacCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGCCGCAAGTCACAAAATCTGCGCATAATAATATCAAAACGACGTCGAATTGATAGTCGTTCTCATTACTATTTGTATACTGCCGTACCTTTg                >  1:902307/1‑150 (MQ=255)
                                                                                                                                    atacCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAACGACGTCGAATTGATAGTCGTTCTCATTACTATTTGCATACTGCCGTACCTTTGc               >  2:753786/1‑150 (MQ=255)
                                                                                                                                    atacCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAACGACGTCGAATTGATAGTCGTTCTCATTACTATTTGCATACTGCCGTACCTTTGc               >  1:383004/1‑150 (MQ=255)
                                                                                                                                     tacCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAACGACGTCGAATTGATAGTCGTTCTCATTACTATTTGCATACTGCCGTACCTTTGct              <  1:556113/150‑1 (MQ=255)
                                                                                                                                      acCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAACTACGTCGAATTGATAGTCGTTCTCATTACTATTTGCATACTGCCGTACCTTTGctt             <  2:96532/150‑1 (MQ=255)
                                                                                                                                         ccAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAACGACGTCGAATTGATAGTCGTTCTCATTACTATTTGCATACTGCCGTACCTTTGctttct          <  1:1653711/150‑1 (MQ=255)
                                                                                                                                         ccAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAACGACGTCGAATTGATAGTCGTTCTCATTACTATTTGCATACTGCCGTACCTTTGctttct          <  1:223888/150‑1 (MQ=255)
                                                                                                                                          cAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAACGACGTCGAATTGATAGTCGTTCTCATTACTATTTGCATACTGCCGTACCTTTGctttctt         >  1:1379031/1‑150 (MQ=255)
                                                                                                                                               gcaaaGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATTTCAAAACGACGTCGAATTGATAGTCGTTCTCATTACTATTTGCATACTGCCGTACCTTTGCTTTCTTTTCCt    <  2:240987/148‑1 (MQ=255)
                                                                                                                                                gAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAACGACGTCGAATTGATAGTCGTTCTCATTACTATTTGCATACTGCCGTACCTTTGCTTTCTTTTCCtt   >  1:360344/1‑150 (MQ=255)
                                                                                                                                                gAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAACGACGTCGAATTGATAGTCGTTCTCATTACTATTTGCATACTGCCGTACCTTTGCTTTCTTTTCCtt   >  1:573862/1‑150 (MQ=255)
                                                                                                                                                gAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAACGACGTCGAATTGATAGTCGTTCTCATTACTATTTGCATACTGCCGTACCTTTGCTTTCTTTTCCtt   >  1:580682/1‑150 (MQ=255)
                                                                                                                                                 aaaGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAACGACGTCGAATTGATAGTCGTTCTCATTACTATTTGCATACTGCCGTACCTTTGCTTTCTTTTCCTTg  <  2:724003/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GCTCGCGCTTAAGGCGACGCTTCTGATTGAAGAACTCTACGCTCTTACTGAAGAAGATTGCCCAGGTGACTACGGAGGCCAAAATAAGCCCAATCATCACGCACTTAACGACAATATCGGCGTGCTGATACATACCCCAGACGGAAAGGTCCGTCTGCATTAAATTATTACCCACTGTGTATCTCCAGGACGCAAGTCACAAAATCTGCGCATAATAATATCAAAACGACGTCGAATTGATAGTCGTTCTCATTACTATTTGCATACTGCCGTACCTTTGCTTTCTTTTCCTTG  >  NC_000913/3151810‑3152103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: