Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,155,230 T→G 9.1% intergenic (‑12/‑125) yqhC ← / → yqhD transcriptional activator of yqhD/aldehyde reductase, NADPH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,155,2300TG 9.1% 431.0 / 28.5 151intergenic (‑12/‑125)yqhC/yqhDtranscriptional activator of yqhD/aldehyde reductase, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base T (75/62);  new base G (6/8);  total (81/70)
Fisher's exact test for biased strand distribution p-value = 4.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.23e-01

Only 100 of 151 total aligned reads displayed.

CTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTGCAC  >  NC_000913/3155086‑3155375
                                                                                                                                                |                                                                                                                                                 
    cAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGaaa                                                                                                                                          <  1:36950/150‑1 (MQ=255)
    cAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGAGTGCTGGAGaaa                                                                                                                                          <  2:412627/150‑1 (MQ=255)
     aaaCTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCTAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGAGTGCTGGAGAAAt                                                                                                                                         <  2:1157690/150‑1 (MQ=255)
      aaCTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATa                                                                                                                                        >  1:1337154/1‑150 (MQ=255)
      aaCTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGAGTGCTGGAGAAAtg                                                                                                                                        <  2:746298/150‑2 (MQ=255)
      aaCTATTTTATTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGAGTGCTGGAGAAAtg                                                                                                                                        <  2:736882/150‑2 (MQ=255)
        cTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAgg                                                                                                                                      <  2:1210210/150‑1 (MQ=255)
        cTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGAGTGCTGGAGAAAtgct                                                                                                                                      <  2:189919/150‑4 (MQ=255)
        cTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTAACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTATCCAGAACTCTGGAGAAATAgg                                                                                                                                      >  1:391834/1‑150 (MQ=255)
          aTTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCa                                                                                                                                    <  2:541432/150‑1 (MQ=255)
          aTTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCa                                                                                                                                    >  1:1541622/1‑150 (MQ=255)
          aTTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCa                                                                                                                                    <  1:1533563/150‑1 (MQ=255)
          aTTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGAGTGCTGGAGAAAtgctac                                                                                                                                    <  1:960638/150‑6 (MQ=255)
           ttttCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGAGTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGcta                                                                                                                                   >  2:466064/1‑148 (MQ=255)
             ttCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGa                                                                                                                                 <  1:133041/150‑1 (MQ=255)
                tttATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACAt                                                                                                                              >  1:479709/1‑150 (MQ=255)
                 ttATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACAtt                                                                                                                             >  2:633899/1‑150 (MQ=255)
                 ttATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAAGCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACAtt                                                                                                                             <  1:1265171/150‑1 (MQ=255)
                  tATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTg                                                                                                                            >  2:1701006/1‑150 (MQ=255)
                  tATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTg                                                                                                                            >  1:1242813/1‑150 (MQ=255)
                  tATTTTTCAGTTTATTAACTATATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTg                                                                                                                            <  1:342672/150‑1 (MQ=255)
                   aTTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTgg                                                                                                                           <  2:324118/150‑1 (MQ=255)
                   aTTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTgg                                                                                                                           <  1:640602/150‑1 (MQ=255)
                   aTTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGAGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTgg                                                                                                                           <  2:1092475/150‑1 (MQ=255)
                   aTTTTTCAGTTTATTAACTTTATCCGCCAACAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTgg                                                                                                                           <  2:1392023/150‑1 (MQ=255)
                    tttttCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGTAATTATGCGTCAATTTGATTGTGCGCAATCTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGc                                                                                                                          <  1:70473/150‑1 (MQ=255)
                    tttttCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGc                                                                                                                          <  2:1039304/150‑1 (MQ=255)
                      tttCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAg                                                                                                                        <  2:1416234/150‑1 (MQ=255)
                      tttCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAg                                                                                                                        <  2:1337154/150‑1 (MQ=255)
                         cAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGaaa                                                                                                                     >  1:424755/1‑150 (MQ=255)
                         cAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGaaa                                                                                                                     >  1:440832/1‑150 (MQ=255)
                           gTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATg                                                                                                                   >  2:939094/1‑150 (MQ=255)
                            tttATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGa                                                                                                                  >  1:417409/1‑150 (MQ=255)
                             ttattaACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAg                                                                                                                 <  2:424755/150‑1 (MQ=255)
                              tattaACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGc                                                                                                                >  2:230615/1‑150 (MQ=255)
                                ttaACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCAt                                                                                                              >  1:1152888/1‑150 (MQ=255)
                                 taACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCTGAAATGAGCAtt                                                                                                             >  2:1269674/1‑150 (MQ=255)
                                 taACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCAtt                                                                                                             >  2:347608/1‑150 (MQ=255)
                                  aaCTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTg                                                                                                            <  1:551070/150‑1 (MQ=255)
                                   aCTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGAAGAAATTAGCATTga                                                                                                           >  1:534624/1‑150 (MQ=255)
                                    cTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTgag                                                                                                          >  1:446521/1‑150 (MQ=255)
                                        aTCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGcc                                                                                                      <  2:601565/150‑1 (MQ=255)
                                         tCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCa                                                                                                     >  2:777642/1‑150 (MQ=255)
                                          ccGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAg                                                                                                    >  2:484763/1‑150 (MQ=255)
                                           cGCCAGCAAGCGGCAAATCTCTTCACGTTTCATCTCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGATATAGGCAATACATTGGCAGAAATGAGCATTGAGAGCCAgg                                                                                                   >  2:825499/1‑150 (MQ=255)
                                                 cAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGCGCCAGGGCGCTg                                                                                             >  2:166073/1‑150 (MQ=255)
                                                    gCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTTCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCg                                                                                          <  2:1089173/150‑1 (MQ=255)
                                                    gCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCg                                                                                          >  1:543677/1‑150 (MQ=255)
                                                       gCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATc                                                                                       <  2:1699686/150‑1 (MQ=255)
                                                       gCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATc                                                                                       <  1:484763/150‑1 (MQ=255)
                                                         aaaTCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATcac                                                                                     <  2:1193140/150‑1 (MQ=255)
                                                             ctctTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATg                                                                                 >  1:1512150/1‑150 (MQ=255)
                                                               ctTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAAATTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGaa                                                                               >  2:159033/1‑150 (MQ=255)
                                                                   aCGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAAc                                                                           <  2:141674/150‑1 (MQ=255)
                                                                    cGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGAGTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACa                                                                          <  1:1151613/150‑1 (MQ=255)
                                                                          atatCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAggt                                                                    <  1:818611/150‑2 (MQ=255)
                                                                          atatCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGc                                                                    <  2:1512150/150‑1 (MQ=255)
                                                                          atatCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGc                                                                    <  1:517902/150‑1 (MQ=255)
                                                                           tatCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCa                                                                   >  2:258609/1‑150 (MQ=255)
                                                                            atCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAg                                                                  >  1:659304/1‑150 (MQ=255)
                                                                                cgTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCg                                                              >  1:1479931/1‑150 (MQ=255)
                                                                                   aaTTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTtc                                                           <  1:995786/150‑1 (MQ=255)
                                                                                     tttCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTtctc                                                         <  1:921417/150‑1 (MQ=255)
                                                                                      ttCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTctct                                                        <  1:670620/150‑1 (MQ=255)
                                                                                       tCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTg                                                       <  2:177856/150‑1 (MQ=255)
                                                                                        cTTAGGAATAATGCGGCAATTTGATTGTGTGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGc                                                      >  1:499483/1‑150 (MQ=255)
                                                                                          tAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGccc                                                    <  2:367885/150‑1 (MQ=255)
                                                                                          tAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGccc                                                    >  1:96270/1‑150 (MQ=255)
                                                                                           aGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCt                                                   <  1:230615/150‑1 (MQ=255)
                                                                                           aGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCt                                                   >  1:243118/1‑150 (MQ=255)
                                                                                            ggAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTc                                                  <  2:1291658/150‑1 (MQ=255)
                                                                                            ggAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTc                                                  <  2:1242813/150‑1 (MQ=255)
                                                                                                   tGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTgg                                           >  1:112881/1‑150 (MQ=255)
                                                                                                     cGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCTGAAATGAGCATTGAGAGCCAGGGCGATGGCGAACACAATGAAAAACAACAGGAAGATCTTGCTCTGCCCGCATATTGGcc                                         >  2:645917/1‑150 (MQ=255)
                                                                                                       gCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCa                                       <  1:347880/150‑1 (MQ=255)
                                                                                                        cAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTTGCCCAg                                      >  1:83378/1‑150 (MQ=255)
                                                                                                        cAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAg                                      >  2:801627/1‑150 (MQ=255)
                                                                                                            ttGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCaaa                                  <  2:391834/150‑1 (MQ=255)
                                                                                                               aTTGTGCGCATTTTTGTAGCATTTCTCCTGCACTCTTGAGAAATAGGCAAGACATTGGCAGAAAGGAGGATTGAGAGCCAGGGCGCTGGCGATCTCAATGAAAACGATCAGGCAGATCGTTGTCTGCCCTCATATTGGCCCAGCAAAggg                               <  1:315223/150‑1 (MQ=255)
                                                                                                                  gtgCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGc                            >  1:1035348/1‑150 (MQ=255)
                                                                                                                   tgCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCa                           >  1:1613214/1‑150 (MQ=255)
                                                                                                                     cgcAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAg                         >  1:1131208/1‑150 (MQ=255)
                                                                                                                      gcAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGt                        >  1:1392425/1‑150 (MQ=255)
                                                                                                                        aaTTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTaa                      <  1:521264/150‑1 (MQ=255)
                                                                                                                         aTTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAAt                     <  2:543677/150‑1 (MQ=255)
                                                                                                                           tttGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGa                   <  1:159033/150‑1 (MQ=255)
                                                                                                                             ttgcctaTTTCTCCAGAGTGCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGAGCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGaac                 >  1:88130/7‑150 (MQ=255)
                                                                                                                               tAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGaacaa               >  2:1202253/1‑150 (MQ=255)
                                                                                                                                cctaTTTCTCCAGAGTGCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGaacaac              >  1:1425774/4‑150 (MQ=255)
                                                                                                                                aGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGaacaac              >  2:1323702/1‑150 (MQ=255)
                                                                                                                                 ctaTTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACt             >  1:303020/3‑150 (MQ=255)
                                                                                                                                  cATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACtt            >  2:575788/1‑150 (MQ=255)
                                                                                                                                   aTTTCTCCAGAGTGCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACttt           >  2:888743/1‑150 (MQ=255)
                                                                                                                                    tttCTCCAGAGTGCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTa          >  1:811059/1‑150 (MQ=255)
                                                                                                                                         ccAGCACTCTGGAGAAATAGGCTAGACATTTGCAGAAATGATCATTGAGAGCCAGGGCGCTGGCGATCACAATTAAAAACATCAGGCAGATCGTTCTCTGCCCTCATTTTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTg     <  1:1373853/150‑1 (MQ=255)
                                                                                                                                         ccAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTg     >  1:1499302/1‑150 (MQ=255)
                                                                                                                                         ccAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTg     >  1:842518/1‑150 (MQ=255)
                                                                                                                                         ccAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTg     >  2:1509342/1‑150 (MQ=255)
                                                                                                                                         ccAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTg     >  1:1057215/1‑150 (MQ=255)
                                                                                                                                          cAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTGc    >  1:886467/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
CTGACAAACTATTTTCTTTATTTTTCAGTTTATTAACTTTATCCGCCAGCAAGCGGCAAATCTCTTCACGTTTCATATCGCGTAATTTCTTAGGAATAATGCGGCAATTTGATTGTGCGCAATTTTGTAGCATTTCTCCAGCACTCTGGAGAAATAGGCAAGACATTGGCAGAAATGAGCATTGAGAGCCAGGGCGCTGGCGATCACAATGAAAAACATCAGGCAGATCGTTCTCTGCCCTCATATTGGCCCAGCAAAGGGAGCAAGTAATGAACAACTTTAATCTGCAC  >  NC_000913/3155086‑3155375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: