Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,156,590 C→A 7.6% intergenic (+72/‑33) yqhD → / → dkgA aldehyde reductase, NADPH‑dependent/2,5‑diketo‑D‑gluconate reductase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,156,5900CA 7.6% 306.1 / 12.6 105intergenic (+72/‑33)yqhD/dkgAaldehyde reductase, NADPH‑dependent/2,5‑diketo‑D‑gluconate reductase A
Reads supporting (aligned to +/- strand):  ref base C (39/58);  new base A (3/5);  total (42/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.35e-01

Only 100 of 108 total aligned reads displayed.

CACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCAAAAAGCGTTAGA  >  NC_000913/3156441‑3156738
                                                                                                                                                     |                                                                                                                                                    
cACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGcc                                                                                                                                                      <  2:1409495/150‑1 (MQ=255)
  cGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTg                                                                                                                                                    >  1:239930/1‑150 (MQ=255)
    gCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCt                                                                                                                                                  <  2:1220427/150‑1 (MQ=255)
     cATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACATCACCTCACCGGAGCCTGCTc                                                                                                                                                 >  2:446319/1‑150 (MQ=255)
      aTGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTcc                                                                                                                                                <  2:1066028/150‑1 (MQ=255)
       tGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTACGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACCCCTCACCGGAGCCTGCTCCg                                                                                                                                               >  1:1132250/1‑150 (MQ=255)
        gACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCgg                                                                                                                                              >  2:1231961/1‑150 (MQ=255)
         aCCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGt                                                                                                                                             >  2:285833/1‑150 (MQ=255)
          cccAACTGGGCTAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCAGTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCAGGCTCCGGTg                                                                                                                                            <  1:261373/150‑1 (MQ=255)
          cccAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTg                                                                                                                                            <  2:1554869/150‑1 (MQ=255)
          cccAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTg                                                                                                                                            <  1:201012/150‑1 (MQ=255)
          cccAACTGGGCGAAAATCATGACATTACGTTGGACGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCAGGCTCCGGTg                                                                                                                                            <  1:1011156/150‑1 (MQ=255)
            cAACTGGGCGAAAATCATTAAATTACGTTGGATTTCAGCCCCCGTATATACGAAGCCGCCCGCTAAGCTTTTTAAGCCTAAAACATTAGTTTTAGGGCATTTAGTCCAGACTTAAGTTCAAAAAACCTAACCGGAGCCTTCTCCGGTGat                                                                                                                                          >  2:1437114/1‑149 (MQ=255)
            cAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAg                                                                                                                                          <  1:309321/150‑1 (MQ=255)
            cAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCAGGCTCCGGTGAg                                                                                                                                          <  1:1654627/150‑1 (MQ=255)
             aaCTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCAGGCTCCGGTGAgg                                                                                                                                         <  1:1011926/150‑2 (MQ=255)
                    cGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCCGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCAtata                                                                                                                                  >  1:1609469/1‑150 (MQ=255)
                    cGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCAtata                                                                                                                                  <  2:706380/150‑1 (MQ=255)
                    cGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCAtata                                                                                                                                  >  1:408068/1‑150 (MQ=255)
                       aaaTCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAag                                                                                                                               <  2:1630982/150‑1 (MQ=255)
                         aTCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAagga                                                                                                                             >  1:733837/1‑150 (MQ=255)
                            aTGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAggagga                                                                                                                          >  2:448872/1‑150 (MQ=255)
                               aCATTACGTTGGTTGGCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCGCAAACATTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACg                                                                                                                       <  2:223527/150‑1 (MQ=255)
                                 aTTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTa                                                                                                                     <  1:741/150‑1 (MQ=255)
                                 aTTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTa                                                                                                                     >  1:207218/1‑150 (MQ=255)
                                 aTTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTATTTACGCCTCAAACTTTCGTTTTAGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATTTAAAGGAGGAACGTa                                                                                                                     >  1:1516508/1‑150 (MQ=255)
                                   tACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATg                                                                                                                   <  1:1385734/150‑1 (MQ=255)
                                     cGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGc                                                                                                                 <  2:408068/150‑1 (MQ=255)
                                     cGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGc                                                                                                                 >  1:636104/1‑150 (MQ=255)
                                       ttGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGTAATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTa                                                                                                               <  1:1437114/150‑1 (MQ=255)
                                       ttGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTa                                                                                                               >  1:27830/1‑150 (MQ=255)
                                            tGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGATTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCa                                                                                                          <  2:1060483/150‑1 (MQ=255)
                                              tCATCCGACGTATATACCAAGCCTCCCGCTAAGCTTTTTACGCCTCAAACTTTCGATTTCGTGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAAc                                                                                                        <  1:347203/150‑1 (MQ=255)
                                              tCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAAc                                                                                                        <  1:1608061/150‑1 (MQ=255)
                                               cAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAAcc                                                                                                       >  2:29160/1‑150 (MQ=255)
                                                  ccgccgTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGtt                                                                                                    >  2:1119851/1‑150 (MQ=255)
                                                  ccgccgTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGtt                                                                                                    <  2:836585/150‑1 (MQ=255)
                                                  ccgccgTATATACGAAGCCGCCCGCCAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGtt                                                                                                    <  2:377946/150‑1 (MQ=255)
                                                      cgTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGttatta                                                                                                >  2:61677/1‑150 (MQ=255)
                                                         atataCGAAGCCGCCAGCTAAGCTTTTTACGCCGCAAACTTTCGTTTTCGGGCATTTAGTCCAGACTTAAGTTCACAACACCTCACCGGCGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGc                                                                                             <  2:301380/150‑1 (MQ=255)
                                                          tataCGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCt                                                                                            <  2:492810/150‑1 (MQ=255)
                                                           ataCGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTa                                                                                           >  1:1238044/1‑150 (MQ=255)
                                                            taCGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTGCGGGCATTTCGTCCAGACTTACGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTAc                                                                                          <  2:1226385/150‑1 (MQ=255)
                                                             aCGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACa                                                                                         <  1:638947/150‑1 (MQ=255)
                                                                aaGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGa                                                                                      >  1:1202096/1‑150 (MQ=255)
                                                                aaGCCCCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGa                                                                                      <  2:158937/150‑1 (MQ=255)
                                                                    cgccctcTTAGCTTTTTAAGCCTCAAACTTTGGTTTTCGGTCATTTCTTCCATACTTAAGTTCACTACTCCTCAACGGATCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGTATGGc                                                                                  <  1:444277/150‑1 (MQ=255)
                                                                     gcccgcTAAGCTTTTTACGCCTCATACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATTTAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCa                                                                                 >  1:1613309/1‑150 (MQ=255)
                                                                     gcccgcTAAGCTTTTTACGCCTCAAACTTTAGTTTTCGGGCATTTCGTCCAGACTTAAGTTAACAACACCACACCGGAGCCTGCTCCGGTGAGTTCGTATAAATGAGGAACGTATCGCTAATCCAACCGTTATAAAGCTACAGGATGCCa                                                                                 >  2:1468810/1‑150 (MQ=255)
                                                                        cgcTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATg                                                                              >  1:639011/1‑150 (MQ=255)
                                                                         gcTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATAGAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGt                                                                             <  2:1377273/150‑1 (MQ=255)
                                                                          cTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTc                                                                            <  2:239930/150‑1 (MQ=255)
                                                                           tAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCa                                                                           <  2:207218/150‑1 (MQ=255)
                                                                               cTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGcc                                                                       >  1:515767/1‑150 (MQ=255)
                                                                               cTTTTTACGCCTCAAACTTTCGGTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGcc                                                                       <  2:44778/150‑1 (MQ=255)
                                                                                 ttttACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTTAGCTACAGGATGGCAATGTCATGcccc                                                                     >  1:565705/1‑150 (MQ=255)
                                                                                   ttACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAg                                                                   >  2:1391763/1‑150 (MQ=255)
                                                                                     aCGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCGTATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCt                                                                 <  1:1468810/150‑1 (MQ=255)
                                                                                      cGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTg                                                                <  2:1425346/150‑1 (MQ=255)
                                                                                      cGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTg                                                                <  2:1086325/150‑1 (MQ=255)
                                                                                       gacTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTgg                                                               <  1:679451/148‑1 (MQ=255)
                                                                                       cccTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTgg                                                               <  1:590606/149‑1 (MQ=255)
                                                                                        ccTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTggg                                                              <  1:365223/150‑1 (MQ=255)
                                                                                          tCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGAc                                                            >  1:1084466/1‑150 (MQ=255)
                                                                                           cAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCAACTCCAACCGTTATTAAGCTACAGGAGGGCAATGTCATGCCCCAGCTGGGACt                                                           <  1:285833/150‑1 (MQ=255)
                                                                                               cTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTggcc                                                       >  1:168028/1‑148 (MQ=255)
                                                                                               cTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGc                                                       >  1:1031299/1‑150 (MQ=255)
                                                                                                tttCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCg                                                      >  1:1122787/1‑150 (MQ=255)
                                                                                                 ttCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCAGGTGAGTTGATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGt                                                     >  1:1321763/1‑150 (MQ=255)
                                                                                                    gTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTg                                                  <  1:61677/150‑1 (MQ=255)
                                                                                                     ttttCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTgg                                                 >  2:207889/1‑150 (MQ=255)
                                                                                                      tttCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGgc                                                <  1:1652428/150‑1 (MQ=255)
                                                                                                       ttCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGgca                                               <  2:1319498/150‑1 (MQ=255)
                                                                                                       ttCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGgca                                               <  1:566700/150‑1 (MQ=255)
                                                                                                         cGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGgcaag                                             <  2:205619/150‑1 (MQ=255)
                                                                                                             cATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGcaagcaag                                         <  2:801471/150‑1 (MQ=255)
                                                                                                               tttCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCCGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTa                                       <  1:696940/150‑1 (MQ=255)
                                                                                                                   gTCCTGACTTAAGTTCACAACAGCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGa                                   <  2:858307/150‑1 (MQ=255)
                                                                                                                   gTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGa                                   >  1:1340939/1‑150 (MQ=255)
                                                                                                                   gTCCAGACTTAACGTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGa                                   <  2:462265/150‑1 (MQ=255)
                                                                                                                    tCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAg                                  <  1:1231961/150‑1 (MQ=255)
                                                                                                                    tCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAg                                  <  2:1238044/150‑1 (MQ=255)
                                                                                                                        gACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAg                              >  1:1576820/1‑150 (MQ=255)
                                                                                                                          cTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTa                            >  1:1442286/1‑150 (MQ=255)
                                                                                                                               gTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCAc                       >  2:923627/1‑150 (MQ=255)
                                                                                                                                   acaacaCCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGcc                   <  2:1122787/150‑1 (MQ=255)
                                                                                                                                   acaacaCCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGcc                   <  1:963796/150‑1 (MQ=255)
                                                                                                                                     aacaCCTCACCGGAGGCTTCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTTTTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCAt                 <  2:1132250/150‑1 (MQ=255)
                                                                                                                                      acacCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGATGAACGTATGGCTAATCCAACAGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACGGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCAACTCCAtt                <  2:1516508/150‑1 (MQ=255)
                                                                                                                                       cacCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCAAGCTGGGACTGGGCGTCTGGAAAGCAACTAAAGAGGAAGTAATCACCGCCATTc               >  2:269334/1‑150 (MQ=255)
                                                                                                                                         ccTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGTGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCaa             <  2:27830/150‑1 (MQ=255)
                                                                                                                                         ccTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCaa             >  2:1456479/1‑150 (MQ=255)
                                                                                                                                          cTCACCGGAGCCTGCTCCGGTGAGTTCTTATATTGTAGGAACGTATGGCTAATCCATCCGTTATTAAGCTACATGATGGCAATGTCATGCGCCAGCTGGGACTTGGCGTCTGGCAAGCAAGTATTGATGAAGTAATCACCGCCATTCaaa            <  1:858838/150‑1 (MQ=255)
                                                                                                                                          cTCACCGGAGCAGGCTCCGGTGAGTTGAAATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTTAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCATGCAAGTAATGAGGAAGTAATCACCGCCATTCaaa            >  1:751643/1‑150 (MQ=255)
                                                                                                                                          cTCACCGGAGCAGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCaaa            >  1:1686749/1‑150 (MQ=255)
                                                                                                                                            cACCGGAGCAGGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTAGTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTCATCACCGCCATTCaaaaa          >  1:1100774/1‑150 (MQ=255)
                                                                                                                                                ggAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTTCAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCAAAAAGCGt      <  1:446319/150‑1 (MQ=255)
                                                                                                                                                   gCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCAAAAAGCGTTAg   <  2:1202096/150‑1 (MQ=255)
                                                                                                                                                    ccTTCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCAAAAAGCGTTAGa  <  2:187251/150‑1 (MQ=255)
                                                                                                                                                    ccTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCAAAAAGCGTTAGa  <  2:193121/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATATACGAAGCCGCCCGCTAAGCTTTTTACGCCTCAAACTTTCGTTTTCGGGCATTTCGTCCAGACTTAAGTTCACAACACCTCACCGGAGCCTGCTCCGGTGAGTTCATATAAAGGAGGAACGTATGGCTAATCCAACCGTTATTAAGCTACAGGATGGCAATGTCATGCCCCAGCTGGGACTGGGCGTCTGGCAAGCAAGTAATGAGGAAGTAATCACCGCCATTCAAAAAGCGTTAGA  >  NC_000913/3156441‑3156738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: