Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,172,439 A→T 5.8% intergenic (‑228/‑91) ygiZ ← / → mdaB inner membrane protein/NADPH quinone reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,172,4390AT 5.8% 328.3 / 5.0 110intergenic (‑228/‑91)ygiZ/mdaBinner membrane protein/NADPH quinone reductase
Reads supporting (aligned to +/- strand):  ref base A (51/52);  new base T (2/5);  total (53/57)
Fisher's exact test for biased strand distribution p-value = 4.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

TATTTTCAATCCCCAAAAAAACCGAACCAAACGTGTAAATTATTCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGCCCACTCCAATGGTCAACT  >  NC_000913/3172293‑3172588
                                                                                                                                                  |                                                                                                                                                     
tatTTTCAATCCCCAAAAAAACCGAACCAAACGTGTAAATTATTCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTc                                                                                                                                                    <  2:1373471/150‑1 (MQ=255)
 atTTTCAATCCCCAAAAAAACCGAACCAAACGTGTAAATTATTCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCg                                                                                                                                                   <  2:622483/150‑1 (MQ=255)
 atTTTCAATCCCCAAAAAAACCGAACCAAACGTGTAAATTATTCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCg                                                                                                                                                   <  1:1666238/150‑1 (MQ=255)
 atTTTCAATCCCCAAAAAAACCGAACCAAACGTGTAAATTATTCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATAATGCATCGTTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCg                                                                                                                                                   >  2:1403380/1‑150 (MQ=255)
 atTTTCAATCCACAAAAAAACCGAACCAAACGTGTAAATTATTCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATACTTCATCTTTGAGTCAGAGTAAGATCATACTTTTTCTAAATTCg                                                                                                                                                   >  2:456074/1‑150 (MQ=255)
  ttttCAATCCCCAAAAAAACCGAACCAAACGTGTAAATTATTCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGc                                                                                                                                                  >  2:1028598/1‑150 (MQ=255)
    ttCAATCCCCAAAAAAACCGACCCAAACGTGTAAATTATTCCGGTGTGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCTTTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCaa                                                                                                                                                >  2:103039/1‑150 (MQ=255)
     tCAATCCCCAAAAAAACCGAACCAAACGTGTAAATTATTCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCaaa                                                                                                                                               >  2:1534710/1‑150 (MQ=255)
       aaTCCCCAAAAAAACCGAACCAAACGTGTAAATTATTCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAg                                                                                                                                             <  2:173764/150‑1 (MQ=255)
        aTCCCCAAAAAAACCGAACCAAACGTGTAAATTATTCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGa                                                                                                                                            >  2:1108834/1‑150 (MQ=255)
         tCCCCAAAAAAACCGAACCAAACGTGTAAATTATTCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCAGCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGAc                                                                                                                                           <  1:1547710/150‑1 (MQ=255)
          ccccAAAAAAACCGAACCAAACGTGTAAATTATTCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCGAATTTAGCAAAAGTCt                                                                                                                                          <  1:1338116/150‑1 (MQ=255)
            ccAAAAAAACCGAACCAAACGTGTAAATTATTCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACttt                                                                                                                                        >  1:844540/1‑150 (MQ=255)
            ccAAAAAAACCGAACCAAACGTGTAAATTATTCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACttt                                                                                                                                        <  1:1437345/150‑1 (MQ=255)
             cAAAAAAACCTAACCAAACGTGTAAATTATTCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGTCTTTTGCGAATTTAGCAAAAGTCtgat                                                                                                                                       <  1:471745/150‑4 (MQ=255)
             cAAAAAAACCGAACCAAACGTGTAAATTATTCCTGTGCTAGTAAGAATTATGAGTAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGTCTTTTGCGAATTTAGCAAAAGTCtgat                                                                                                                                       <  2:1062070/150‑4 (MQ=255)
             cAAAAAAACCGAACCAAACGTGTAAATTATTCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGTCTTTTGCGAATTTAGCAAAAGTCtgat                                                                                                                                       <  1:471741/150‑4 (MQ=255)
             cAAAAAAACCGAACCAAACGTGTAAATTATTCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATACGCAAAAGACTTTg                                                                                                                                       <  2:1378544/150‑1 (MQ=255)
               aaaaaaCCGAACCAAACGTGTAAATTATTCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGca                                                                                                                                     <  1:386223/150‑1 (MQ=255)
                   aaCCGAACCAAACGTGTAAATTATTCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCATAGTAAGATAAGACTTTTGATAAATTCTCAAAATACTTTTCACAtt                                                                                                                                 >  1:112413/1‑150 (MQ=255)
                    aCCGAACCAAACGTGTAAATTATTCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACAttt                                                                                                                                <  1:479330/150‑1 (MQ=255)
                    aCCGAACCAAACGTGTAAATTATTCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACAttt                                                                                                                                <  1:702455/150‑1 (MQ=255)
                     ccGAACCAAACGTGTAAATTATTCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACAtttt                                                                                                                               >  1:1024598/1‑150 (MQ=255)
                     ccGAACCAAACGTGTAAATTATTCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACAtttt                                                                                                                               <  1:1626771/150‑1 (MQ=255)
                     ccGAACCAAACGTGTAAATTATTCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACAtttt                                                                                                                               >  2:607649/1‑150 (MQ=255)
                     ccGAACCAAACGTGTAAATTATTCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACAtttt                                                                                                                               >  1:1274420/1‑150 (MQ=255)
                      cGAACCAAACGTGTAAATTATTCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTg                                                                                                                              >  2:1576523/1‑150 (MQ=255)
                       gAACCAAACGTGTAAATTATTCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGc                                                                                                                             <  2:1552486/150‑1 (MQ=255)
                         aCCAAACGTGTAAATTATTCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTa                                                                                                                           <  1:1121525/150‑1 (MQ=255)
                            aaaCGTGTAAATTATTCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAAtt                                                                                                                        >  1:1133845/1‑150 (MQ=255)
                            aaaCGTGTAAATTATTCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTCCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAAtt                                                                                                                        >  2:311765/1‑150 (MQ=255)
                               cGTGTAAATTATTCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCa                                                                                                                     <  1:505390/150‑1 (MQ=255)
                                gtgtAAATTATTCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCATAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCAc                                                                                                                    <  2:1111564/150‑1 (MQ=255)
                                   tAAATTATTCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCAcctt                                                                                                                 >  1:1493787/1‑148 (MQ=255)
                                   tAAATTATTCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGt                                                                                                                 <  1:1385659/150‑1 (MQ=255)
                                   tAAATTATTCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGt                                                                                                                 >  1:773865/1‑150 (MQ=255)
                                   tAAATTATTCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGt                                                                                                                 <  1:414103/150‑1 (MQ=255)
                                    aaaTTATTCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAAttt                                                                                                                       <  1:238995/143‑1 (MQ=255)
                                    aaaTTATTCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAAttt                                                                                                                       >  2:238995/1‑143 (MQ=255)
                                      attattCCTGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTAcc                                                                                                              <  1:1449764/150‑1 (MQ=255)
                                        tattCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAATTTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGc                                                                                                            >  1:900674/1‑150 (MQ=255)
                                        tattCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCAcc                                                                                                                   >  1:1468367/1‑143 (MQ=255)
                                        tattCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCAcc                                                                                                                   <  2:1468367/143‑1 (MQ=255)
                                        tattCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGc                                                                                                            <  1:1258036/150‑1 (MQ=255)
                                           tCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGctct                                                                                                         <  2:1250487/150‑1 (MQ=255)
                                            ccGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCtg                                                                                                        <  1:685454/150‑1 (MQ=255)
                                              ggTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCtgtg                                                                                                      <  2:949731/150‑1 (MQ=255)
                                               gTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTTCTAAATTCGCAAAAGACTTTGCACATTTTTATAATTACACCGTACCGCTCtgtta                                                                                                     >  1:1614569/1‑148 (MQ=255)
                                                     gTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCTTACCGCTCTGTGACGTACt                                                                                               >  1:586527/1‑150 (MQ=255)
                                                      tAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACta                                                                                              >  1:594424/1‑150 (MQ=255)
                                                         gAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAg                                                                                           >  1:8199/1‑150 (MQ=255)
                                                              aTGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGc                                                                                      >  1:425750/1‑150 (MQ=255)
                                                              aTGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGc                                                                                      <  2:349167/150‑1 (MQ=255)
                                                                  ggAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAAc                                                                                  >  1:196824/1‑150 (MQ=255)
                                                                   gAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACg                                                                                 >  2:851722/1‑150 (MQ=255)
                                                                    aaTGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGt                                                                                >  1:756745/1‑150 (MQ=255)
                                                                     attgCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCATACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGtc                                                                               <  1:1271864/147‑1 (MQ=255)
                                                                        gCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCa                                                                            >  1:998321/1‑150 (MQ=255)
                                                                         atATCAGTTTTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCATACTTATTCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCAc                                                                           <  1:235462/149‑1 (MQ=255)
                                                                          tATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCAcc                                                                          <  1:1210652/150‑1 (MQ=255)
                                                                            tCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCtt                                                                        >  2:79091/1‑150 (MQ=255)
                                                                              aGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGa                                                                      >  1:1097034/1‑150 (MQ=255)
                                                                                    gTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTaa                                                                >  1:1392984/1‑150 (MQ=255)
                                                                                     tCATTTTCAGAAAATATTTATCCTGCCTCGGTTAGTCAGAGTAATATCAGAATTTTGCTAAATTCGCTAAAGACTTTGCACATTTTGCTAATTTCACCGTAACGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTaaa                                                               <  1:1090702/150‑1 (MQ=255)
                                                                                         tttCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAAt                                                           <  2:1024598/150‑1 (MQ=255)
                                                                                            cAGAAAATATTTATCCTTCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAATACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATATTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAg                                                        <  2:75126/150‑1 (MQ=255)
                                                                                            cAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAg                                                        <  1:186716/150‑1 (MQ=255)
                                                                                            aaGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAg                                                        <  1:1360910/149‑1 (MQ=255)
                                                                                                 aaTATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACa                                                   <  2:1508294/150‑1 (MQ=255)
                                                                                                   tatTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATc                                                 >  2:859634/1‑150 (MQ=255)
                                                                                                     tttATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCt                                               <  2:773865/150‑1 (MQ=255)
                                                                                                     tttATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCt                                               >  1:1655467/1‑150 (MQ=255)
                                                                                                       tATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGa                                             <  2:844540/150‑1 (MQ=255)
                                                                                                        aTCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGAt                                            <  2:892671/150‑1 (MQ=255)
                                                                                                         tCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGAtt                                           >  2:20205/1‑150 (MQ=255)
                                                                                                          ccTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTa                                          >  1:122722/1‑150 (MQ=255)
                                                                                                                tCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACg                                    >  2:786691/1‑150 (MQ=255)
                                                                                                                  ggTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGgc                                  >  2:1636547/1‑150 (MQ=255)
                                                                                                                   gTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGgcg                                 <  2:1412805/150‑1 (MQ=255)
                                                                                                                       gTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGaa                             >  2:164238/1‑150 (MQ=255)
                                                                                                                       gTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGaa                             >  2:398535/1‑150 (MQ=255)
                                                                                                                              tAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTc                      <  1:1395260/150‑1 (MQ=255)
                                                                                                                               aaGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCg                     <  1:1661747/150‑1 (MQ=255)
                                                                                                                                aGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGc                    >  1:1589885/1‑150 (MQ=255)
                                                                                                                                  aTCAGACTTTTGCTAATTTAGAAAAAGACTTTTCACATTTTGCTAATTTCACCGTACCGCTCTTTGACGTAGTATAGTCGGCAAACGTCTCTCCTTGAGGTTAAAAAATTAGCAACATCCTGATTATCAACGTCGCGAAAAAATTCGccc                  <  2:1148808/150‑1 (MQ=255)
                                                                                                                                  aTCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGccc                  <  1:1534710/150‑1 (MQ=255)
                                                                                                                                   tCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGCCCa                 >  2:1187359/1‑150 (MQ=255)
                                                                                                                                   tCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACAGTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAAGGGGCAACAACGTTATTTTCCACGGCCGGATAAAAttgccccc                 >  2:1162633/1‑144 (MQ=255)
                                                                                                                                      gtcTTTTGCGAATTTAGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGACTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGCCCACTc              >  1:1083670/3‑150 (MQ=255)
                                                                                                                                      gACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGCCCACTc              >  1:473276/1‑150 (MQ=255)
                                                                                                                                        cTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCGTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGCCCACTCCa            >  1:700007/1‑150 (MQ=255)
                                                                                                                                          tttGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGCCCACTCCAAt          <  2:1097034/150‑1 (MQ=255)
                                                                                                                                            tGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGgc                                  <  1:1421805/124‑1 (MQ=255)
                                                                                                                                            tGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGgc                                  >  2:1421805/1‑124 (MQ=255)
                                                                                                                                            tGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGCCCACTCCAATgg        >  1:1321146/1‑150 (MQ=255)
                                                                                                                                              ttAAATTCGCATAAGACTTTGCACATTTTGCTAATTTCACCGTACCGATCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGTTTATCAACGGCGCGAAAAAATTCGCCCACTCCAATGGTc      <  2:1614569/149‑1 (MQ=255)
                                                                                                                                              cTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGCCCACTCCAATGGTc      >  1:253144/1‑150 (MQ=255)
                                                                                                                                               tAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGCCCACTCCAATGGTCa     >  2:1642700/1‑150 (MQ=255)
                                                                                                                                               tAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGCCCACTCCAATGGTCa     >  2:1118269/1‑150 (MQ=255)
                                                                                                                                                  aTTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGCCCACTCCAATGGTCAACt  >  1:925132/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
TATTTTCAATCCCCAAAAAAACCGAACCAAACGTGTAAATTATTCCGGTGCGAGTAAGAATTATGAGGAATGGCTATCAGTATTGTCATTTTCAGAAAATATTTATCCTGCATCGGTGAGTCAGAGTAAGATCAGACTTTTGCTAAATTCGCAAAAGACTTTGCACATTTTGCTAATTTCACCGTACCGCTCTGTGACGTACTATAGTCGGCAAACGTCTCACCTTGAGGTTAAAAAATGAGCAACATCCTGATTATCAACGGCGCGAAAAAATTCGCCCACTCCAATGGTCAACT  >  NC_000913/3172293‑3172588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: