Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,187,991 T→A 9.5% pseudogene (565/2439 nt) yqiG → pseudogene; fimbrial export usher family;putative membrane; Not classified; putative membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,187,9910TA 9.5% 302.4 / 19.4 116pseudogene (565/2439 nt)yqiGpseudogene; fimbrial export usher family;putative membrane; Not classified; putative membrane protein
Reads supporting (aligned to +/- strand):  ref base T (54/51);  new base A (6/5);  total (60/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.21e-01

Only 100 of 117 total aligned reads displayed.

ACTTTGGAATCATGGTATTGCTGGGGCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTT  >  NC_000913/3187843‑3188139
                                                                                                                                                    |                                                                                                                                                    
aCTTTGGAATCATGGTATTGCTGGGGCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTa                                                                                                                                                     >  2:326045/1‑150 (MQ=255)
 cTTTGGAATCATGGTATTGCTGGTGCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCATAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTAc                                                                                                                                                    <  2:1235248/150‑1 (MQ=255)
    tGGAATCATGGTATTGCTGGGGCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAg                                                                                                                                                 <  2:359621/150‑1 (MQ=255)
      gAATCATGGTATTGCTGGGGCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGGTAATCAGTa                                                                                                                                               <  1:181662/150‑1 (MQ=255)
        aTCATGGTATTGCTGGGGCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACg                                                                                                                                             <  2:927357/150‑1 (MQ=255)
           aTGGTATTGCTGGGGCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATc                                                                                                                                          <  2:900903/150‑1 (MQ=255)
           aTGGTATTGCTGGGGCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATc                                                                                                                                          >  2:1540535/1‑150 (MQ=255)
             ggTATTGCTGGGGCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAg                                                                                                                                        <  2:387999/150‑1 (MQ=255)
              gTATTGCTGGGGCATTTCTTGACTACAATTTATATGCCTCTCATTAGGCACCACATCAGCGCGATAATTCGCAAAATATAACTTCCTATGGGCAGGCTGGGGTTATTCATGGGGCCTGGCGCGTGCGAACTGATTACCAGTACCATCact                                                                                                                                       >  1:316318/1‑148 (MQ=255)
               tATTGCTGGGGCATTTCTTGACTACAATTTATTTGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGTTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTc                                                                                                                                      <  2:1421334/150‑1 (MQ=255)
                aTTGCTGGGGCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCa                                                                                                                                     >  2:119659/1‑150 (MQ=255)
                  tGCTGGGGCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCAtt                                                                                                                                   <  2:1133929/150‑1 (MQ=255)
                  tGCTGGGGCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCAtt                                                                                                                                   >  1:1700398/1‑150 (MQ=255)
                  tGCTGGGGCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCAtt                                                                                                                                   <  1:1429952/150‑1 (MQ=255)
                   gCTGGGTCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGTCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCAttt                                                                                                                                  <  1:100010/150‑1 (MQ=255)
                   gCTGGGGCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCATATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCAttt                                                                                                                                  >  1:144845/1‑150 (MQ=255)
                    cTGGGGCATTTCTTGACTACAATTAATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGCGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGAACTGATTACCAGAACGATCAGTCATTTa                                                                                                                                 >  2:1686033/1‑150 (MQ=255)
                     tGGGGCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTaa                                                                                                                                <  1:776379/150‑1 (MQ=255)
                     tGGGGCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTAAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTaa                                                                                                                                <  2:1021361/150‑1 (MQ=255)
                      ggggCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAAc                                                                                                                               <  1:1710924/150‑1 (MQ=255)
                       gggCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACa                                                                                                                              >  1:535672/1‑150 (MQ=255)
                         gCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAAt                                                                                                                            <  2:408708/150‑1 (MQ=255)
                           aTTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCTCAAATTATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATgg                                                                                                                          <  1:1184156/150‑1 (MQ=255)
                           aTTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATgg                                                                                                                          <  2:1685412/150‑1 (MQ=255)
                            tttCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGc                                                                                                                         >  2:575236/1‑150 (MQ=255)
                            tttCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGc                                                                                                                         <  2:781232/150‑1 (MQ=255)
                            tttCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGc                                                                                                                         <  2:1594737/150‑1 (MQ=255)
                                 tGACTACAATATATATGCCTCTCATTATGCACCACATCAGGGCGAAAATTCGCAAAATATAAGGTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAg                                                                                                                    <  2:1101564/150‑1 (MQ=255)
                                     tACAATTTATATGCCTCTCATTATGCACCACATCATGGCGATAAATCGCAAAATATATGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAg                                                                                                                <  2:1062300/150‑1 (MQ=255)
                                      aCAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAgg                                                                                                               <  2:1409179/150‑1 (MQ=255)
                                       cAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGAGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGc                                                                                                              >  1:530038/1‑150 (MQ=255)
                                           ttATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGAcc                                                                                                          <  2:916548/150‑1 (MQ=255)
                                            tataTGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGGTAATCAGTACgcagg                                                                                                                                         <  1:78043/118‑5 (MQ=255)
                                            tataTGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGGTAATCAGTACgcagg                                                                                                                                         >  2:82514/1‑114 (MQ=255)
                                            tataTGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGGTAATCAGTACgcagg                                                                                                                                         >  2:78043/1‑114 (MQ=255)
                                            tataTGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGGTAATCAGTACgcagg                                                                                                                                         <  1:82514/118‑5 (MQ=255)
                                             atatGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCGGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCaa                                                                                                        <  2:1251714/150‑1 (MQ=255)
                                              tatGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCCACCAAc                                                                                                       >  1:1537965/1‑150 (MQ=255)
                                               atGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAAcc                                                                                                      <  1:597247/150‑1 (MQ=255)
                                                tGCCTCTCATTCTGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGTCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTCATTAACAGTAATATAAGTCATTTAACAATGGCAAAAGCCAGGCTCCGAACCt                                                                                                     >  1:273933/1‑150 (MQ=255)
                                                tGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCt                                                                                                     >  1:1341003/1‑150 (MQ=255)
                                                  ccTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACATgg                                                                                                   <  2:170259/150‑1 (MQ=255)
                                                  ccTCTCATTATGCACCACATCACGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAACCCAGGCGACCAACCTgg                                                                                                   <  1:1671575/150‑1 (MQ=255)
                                                    tctcATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGAt                                                                                                 <  2:583134/150‑1 (MQ=255)
                                                    tctcATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTAGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATATAACAATGGCAAAAGCCAGGCGACCAACCTGGAt                                                                                                 >  1:356342/1‑150 (MQ=255)
                                                      tcATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTTGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGAttt                                                                                               >  2:842709/1‑150 (MQ=255)
                                                        aTTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTc                                                                                             >  1:1277136/1‑150 (MQ=255)
                                                          tATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCcg                                                                                           <  1:326045/150‑1 (MQ=255)
                                                              caccacATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGta                                                                                       >  2:747618/1‑150 (MQ=255)
                                                               accacATCAGGGCGATAATTCGCAAAAAATAAGTTCCTATGGGCAGGCTGGGGTTGATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCAATTAACAATGGCAAAAGCCAGGCGACAAACCTGGATATTCCGCGtat                                                                                      >  1:650311/1‑150 (MQ=255)
                                                                ccacATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGtatt                                                                                     >  1:1298413/1‑150 (MQ=255)
                                                                   caTCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGtatttat                                                                                  >  1:1215041/1‑150 (MQ=255)
                                                                    aTCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGtatttatt                                                                                 >  1:1224689/1‑150 (MQ=255)
                                                                           cGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCg                                                                          >  1:1263022/1‑150 (MQ=255)
                                                                             aTAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGcc                                                                        <  1:1027012/150‑1 (MQ=255)
                                                                             aTAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGcc                                                                        <  1:1336081/150‑1 (MQ=255)
                                                                              tAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGccc                                                                       <  1:1050438/150‑1 (MQ=255)
                                                                                    gCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATccc                                                                 >  2:1364180/1‑150 (MQ=255)
                                                                                    gCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATccc                                                                 >  2:1612849/1‑150 (MQ=255)
                                                                                     cAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGAGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCa                                                                >  2:1646695/1‑150 (MQ=255)
                                                                                     cAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTAGGGGCCTCGCGCCTGCGTACTGATTACCACTACGAACAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCa                                                                >  1:1663217/1‑150 (MQ=255)
                                                                                        aaTATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCa                                                             <  2:528438/150‑1 (MQ=255)
                                                                                        aaTATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCa                                                             >  1:1530876/1‑150 (MQ=255)
                                                                                             aaGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCaatgaa                                                        <  1:695876/150‑1 (MQ=255)
                                                                                                  ccTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCaa                                                   >  2:937485/1‑150 (MQ=255)
                                                                                                     aTGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTGCGCCCAATCCCAGCAATGAATGCAAaac                                                <  1:240648/150‑1 (MQ=255)
                                                                                                        ggCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAaactaa                                             >  1:676306/1‑150 (MQ=255)
                                                                                                          cAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGGCCAGCCTGGATTTTCCGCGTATTTATTTGGTTCGCCCAATCCCAGCATTGAATGCAAgactaact                                           >  2:421077/1‑150 (MQ=255)
                                                                                                          cAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAaactatct                                           >  1:180633/1‑150 (MQ=255)
                                                                                                            ggCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACtat                                         >  2:1719966/1‑150 (MQ=255)
                                                                                                            ggCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACtat                                         >  1:978971/1‑150 (MQ=255)
                                                                                                            ggCTGGGGTTAATCTTGGGGCCTGGAGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCTACCAACCTTGATTTTACGCGTATTTATTTGTTTAGCACAATCCCAGCAATGAATGCAAAAATAACtat                                         >  2:1713459/1‑150 (MQ=255)
                                                                                                             gCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACtata                                        <  1:460716/150‑1 (MQ=255)
                                                                                                             gCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACtata                                        >  1:1381258/1‑150 (MQ=255)
                                                                                                                ggggTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGt                                     <  1:1364180/150‑1 (MQ=255)
                                                                                                                 gggTTAATCTTTGGGCCTGGCGGCTGCGTACTGATTACCAGTACGATCAGTCATTTTACAATGGCAAAATCCAGGAGACCAAGCTGGATTTTCCGCGTATTTATTTGTTTCTCCCAATCCCTGCTATGTATGCAAAACTAACTATAGGTc                                    <  1:1089317/150‑1 (MQ=255)
                                                                                                                 gggTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTc                                    >  2:1124422/1‑150 (MQ=255)
                                                                                                                 gggTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTc                                    >  2:212209/1‑150 (MQ=255)
                                                                                                                 gggTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTc                                    <  2:704917/150‑1 (MQ=255)
                                                                                                                 gggTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTc                                    <  2:144845/150‑1 (MQ=255)
                                                                                                                  ggTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCa                                   >  2:1712373/1‑150 (MQ=255)
                                                                                                                         cTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGAt                            >  1:1465363/1‑150 (MQ=255)
                                                                                                                             gggCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAATCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTg                        <  2:530038/150‑1 (MQ=255)
                                                                                                                                  tGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATcc                   >  1:1033291/1‑150 (MQ=255)
                                                                                                                                    gcgcCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCctc                 <  2:1046033/150‑1 (MQ=255)
                                                                                                                                     cgcCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGACACTGAATCctct                >  2:188369/1‑150 (MQ=255)
                                                                                                                                     cgcCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCACAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCctct                >  1:1519179/1‑150 (MQ=255)
                                                                                                                                       tgatcGTACTGGTAATCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTAt              >  1:1316305/5‑150 (MQ=255)
                                                                                                                                        gatcGTACTGGTAATCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAaacta                                              <  1:1331064/114‑1 (MQ=255)
                                                                                                                                        gatcGTACTGGTAATCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAaacta                                              <  1:849175/114‑1 (MQ=255)
                                                                                                                                        gatcGTACTGGTAATCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAaacta                                              >  2:1331064/4‑117 (MQ=255)
                                                                                                                                        gatcGTACTGGTAATCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTAtt             >  2:69253/4‑150 (MQ=255)
                                                                                                                                          gCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGAATTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTAtttt           <  1:325526/150‑1 (MQ=255)
                                                                                                                                           cGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTc          <  2:533864/150‑1 (MQ=255)
                                                                                                                                            gTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCg         <  2:1298413/150‑1 (MQ=255)
                                                                                                                                             tACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGa        >  1:1116821/1‑150 (MQ=255)
                                                                                                                                             tACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGa        >  1:981762/1‑150 (MQ=255)
                                                                                                                                              aCTGATTACCAGTACGATCTGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGAc       >  2:967062/1‑150 (MQ=255)
                                                                                                                                                tGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGActc     >  1:805721/1‑150 (MQ=255)
                                                                                                                                                   ttACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCttt  >  2:376060/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ACTTTGGAATCATGGTATTGCTGGGGCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTT  >  NC_000913/3187843‑3188139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: