Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,187,993 C→T 9.8% pseudogene (567/2439 nt) yqiG → pseudogene; fimbrial export usher family;putative membrane; Not classified; putative membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,187,9930CT 9.8% 334.6 / 16.2 116pseudogene (567/2439 nt)yqiGpseudogene; fimbrial export usher family;putative membrane; Not classified; putative membrane protein
Reads supporting (aligned to +/- strand):  ref base C (52/50);  new base T (6/5);  total (61/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01

Only 100 of 118 total aligned reads displayed.

CTTTGGAATCATGGTATTGCTGGGGCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCA  >  NC_000913/3187844‑3188142
                                                                                                                                                     |                                                                                                                                                     
cTTTGGAATCATGGTATTGCTGGTGCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCATAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTAc                                                                                                                                                       <  2:1235248/150‑1 (MQ=255)
   tGGAATCATGGTATTGCTGGGGCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAg                                                                                                                                                    <  2:359621/150‑1 (MQ=255)
    ggAATCATGGTATTGCTGGGGCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGt                                                                                                                                                   <  1:682979/150‑1 (MQ=255)
     gAATCATGGTATTGCTGGGGCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGGTAATCAGTa                                                                                                                                                  <  1:181662/150‑1 (MQ=255)
       aTCATGGTATTGCTGGGGCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACg                                                                                                                                                <  2:927357/150‑1 (MQ=255)
          aTGGTATTGCTGGGGCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATc                                                                                                                                             <  2:900903/150‑1 (MQ=255)
            ggTATTGCTGGGGCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAg                                                                                                                                           <  2:387999/150‑1 (MQ=255)
             gTATTGCTGGGGCATTTCTTGACTACAATTTATATGCCTCTCATTAGGCACCACATCAGCGCGATAATTCGCAAAATATAACTTCCTATGGGCAGGCTGGGGTTATTCATGGGGCCTGGCGCGTGCGAACTGATTACCAGTACCATCact                                                                                                                                          >  1:316318/1‑148 (MQ=255)
              tATTGCTGGGGCATTTCTTGACTACAATTTATTTGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGTTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTc                                                                                                                                         <  2:1421334/150‑1 (MQ=255)
               aTTGCTGGGGCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCa                                                                                                                                        >  2:119659/1‑150 (MQ=255)
                 tGCTGGGGCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCAtt                                                                                                                                      <  2:1133929/150‑1 (MQ=255)
                 tGCTGGGGCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCAtt                                                                                                                                      >  1:1700398/1‑150 (MQ=255)
                 tGCTGGGGCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCAtt                                                                                                                                      <  1:1429952/150‑1 (MQ=255)
                  gCTGGGTCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGTCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCAttt                                                                                                                                     <  1:100010/150‑1 (MQ=255)
                  gCTGGGGCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCATATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCAttt                                                                                                                                     >  1:144845/1‑150 (MQ=255)
                   cTGGGGCATTTCTTGACTACAATTAATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGCGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGAACTGATTACCAGAACGATCAGTCATTTa                                                                                                                                    >  2:1686033/1‑150 (MQ=255)
                    tGGGGCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTaa                                                                                                                                   <  1:776379/150‑1 (MQ=255)
                    tGGGGCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTAAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTaa                                                                                                                                   <  2:1021361/150‑1 (MQ=255)
                     ggggCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGAATAATATAAGTTCCTATGGTCAGGCTTGGGTTAATCTTGGGGCCTGTCGCCTTCGTACTGATTACCAGTACGATCAGTCATATAAc                                                                                                                                  >  2:1089317/1‑150 (MQ=255)
                        gCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAAt                                                                                                                               <  2:408708/150‑1 (MQ=255)
                          aTTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCTCAAATTATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATgg                                                                                                                             <  1:1184156/150‑1 (MQ=255)
                          aTTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATgg                                                                                                                             <  2:1685412/150‑1 (MQ=255)
                           tttCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGc                                                                                                                            <  2:781232/150‑1 (MQ=255)
                           tttCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGc                                                                                                                            <  2:1594737/150‑1 (MQ=255)
                           tttCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGc                                                                                                                            >  2:575236/1‑150 (MQ=255)
                            ttCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCa                                                                                                                           >  1:1409179/1‑150 (MQ=255)
                                tGACTACAATATATATGCCTCTCATTATGCACCACATCAGGGCGAAAATTCGCAAAATATAAGGTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAg                                                                                                                       <  2:1101564/150‑1 (MQ=255)
                                    tACAATTTATATGCCTCTCATTATGCACCACATCATGGCGATAAATCGCAAAATATATGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAg                                                                                                                   <  2:1062300/150‑1 (MQ=255)
                                     aCAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAgg                                                                                                                  <  2:1409179/150‑1 (MQ=255)
                                      cAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGAGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGc                                                                                                                 >  1:530038/1‑150 (MQ=255)
                                       aaTTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTACTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGTCTCCTGCGTAATGATTAACAGTACGATCAGTCATTTAACAATGTCAAAAGCCAGTCg                                                                                                                >  1:235247/1‑150 (MQ=255)
                                        aTTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCAATGGCCAGGCTGGGGTTAATCTTGGGGCCTGGCCCCTGCGTACTGATTAGCAGTACGATCACTCAATTAACAATGGCAAAAGCCAGGCGa                                                                                                               >  1:876365/1‑150 (MQ=255)
                                          ttATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGAcc                                                                                                             <  2:916548/150‑1 (MQ=255)
                                           tataTGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGGTAATCAGTACgcagg                                                                                                                                            >  2:78043/1‑114 (MQ=255)
                                           tataTGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGGTAATCAGTACgcagg                                                                                                                                            >  2:82514/1‑114 (MQ=255)
                                           tataTGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGGTAATCAGTACgcagg                                                                                                                                            <  1:82514/118‑5 (MQ=255)
                                            atatGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCGGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCaa                                                                                                           <  2:1251714/150‑1 (MQ=255)
                                              atGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAAcc                                                                                                         <  1:597247/150‑1 (MQ=255)
                                               tGCCTCTCATTCTGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGTCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTCATTAACAGTAATATAAGTCATTTAACAATGGCAAAAGCCAGGCTCCGAACCt                                                                                                        >  1:273933/1‑150 (MQ=255)
                                               tGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCt                                                                                                        >  1:1341003/1‑150 (MQ=255)
                                                 ccTCTCATTATGCACCACATCACGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAACCCAGGCGACCAACCTgg                                                                                                      <  1:1671575/150‑1 (MQ=255)
                                                   tctcATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGAt                                                                                                    <  2:583134/150‑1 (MQ=255)
                                                   tctcATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTAGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATATAACAATGGCAAAAGCCAGGCGACCAACCTGGAt                                                                                                    >  1:356342/1‑150 (MQ=255)
                                                     tcATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTTGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGAttt                                                                                                  >  2:842709/1‑150 (MQ=255)
                                                       aTTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTc                                                                                                >  1:1277136/1‑150 (MQ=255)
                                                         tATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCcg                                                                                              <  1:326045/150‑1 (MQ=255)
                                                             caccacATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGta                                                                                          >  2:747618/1‑150 (MQ=255)
                                                              accacATCAGGGCGATAATTCGCAAAAAATAAGTTCCTATGGGCAGGCTGGGGTTGATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCAATTAACAATGGCAAAAGCCAGGCGACAAACCTGGATATTCCGCGtat                                                                                         >  1:650311/1‑150 (MQ=255)
                                                               ccacATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGtatt                                                                                        >  1:1298413/1‑150 (MQ=255)
                                                                  caTCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGtatttat                                                                                     >  1:1215041/1‑150 (MQ=255)
                                                                  caTCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGtatttat                                                                                     >  1:442198/1‑150 (MQ=255)
                                                                   aTCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGtatttatt                                                                                    >  1:1224689/1‑150 (MQ=255)
                                                                          cGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCg                                                                             >  1:1263022/1‑150 (MQ=255)
                                                                            aTAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGcc                                                                           <  1:1336081/150‑1 (MQ=255)
                                                                            aTAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGcc                                                                           <  1:1027012/150‑1 (MQ=255)
                                                                             tAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGccc                                                                          <  1:1050438/150‑1 (MQ=255)
                                                                                   gCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATccc                                                                    >  2:1612849/1‑150 (MQ=255)
                                                                                   gCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATccc                                                                    >  2:1364180/1‑150 (MQ=255)
                                                                                    cAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCa                                                                   >  2:1470853/1‑150 (MQ=255)
                                                                                    cAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGAGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCa                                                                   >  2:1646695/1‑150 (MQ=255)
                                                                                    cAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTAGGGGCCTCGCGCCTGCGTACTGATTACCACTACGAACAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCa                                                                   >  1:1663217/1‑150 (MQ=255)
                                                                                       aaTATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCa                                                                >  1:1530876/1‑150 (MQ=255)
                                                                                            aaGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCaatgaa                                                           <  1:695876/150‑1 (MQ=255)
                                                                                             aGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCaatgaat                                                          <  2:1694820/150‑1 (MQ=255)
                                                                                                tCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCa                                                       <  1:1540535/150‑1 (MQ=255)
                                                                                                 ccTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCaa                                                      >  2:937485/1‑150 (MQ=255)
                                                                                                    aTGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTGCGCCCAATCCCAGCAATGAATGCAAaac                                                   <  1:240648/150‑1 (MQ=255)
                                                                                                       ggCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAaactaa                                                >  1:676306/1‑150 (MQ=255)
                                                                                                         cAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGGCCAGCCTGGATTTTCCGCGTATTTATTTGGTTCGCCCAATCCCAGCATTGAATGCAAgactaact                                              >  2:421077/1‑150 (MQ=255)
                                                                                                         cAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAaactatct                                              >  1:180633/1‑150 (MQ=255)
                                                                                                           ggCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACtat                                            >  2:1719966/1‑150 (MQ=255)
                                                                                                           ggCTGGGGTTAATCTTGGGGCCTGGAGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCTACCAACCTTGATTTTACGCGTATTTATTTGTTTAGCACAATCCCAGCAATGAATGCAAAAATAACtat                                            >  2:1713459/1‑150 (MQ=255)
                                                                                                            gCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACtata                                           <  1:460716/150‑1 (MQ=255)
                                                                                                               ggggTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGt                                        <  1:1364180/150‑1 (MQ=255)
                                                                                                                gggTTAATCTTTGGGCCTGGCGGCTGCGTACTGATTACCAGTACGATCAGTCATTTTACAATGGCAAAATCCAGGAGACCAAGCTGGATTTTCCGCGTATTTATTTGTTTCTCCCAATCCCTGCTATGTATGCAAAACTAACTATAGGTc                                       <  1:1089317/150‑1 (MQ=255)
                                                                                                                gggTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTc                                       <  2:704917/150‑1 (MQ=255)
                                                                                                                gggTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTc                                       >  2:212209/1‑150 (MQ=255)
                                                                                                                gggTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTc                                       <  2:144845/150‑1 (MQ=255)
                                                                                                                 ggTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCa                                      >  2:1712373/1‑150 (MQ=255)
                                                                                                                     aaTCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATAc                                  <  2:442198/150‑1 (MQ=255)
                                                                                                                        cTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGAt                               >  1:1465363/1‑150 (MQ=255)
                                                                                                                            gggCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAATCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTg                           <  2:530038/150‑1 (MQ=255)
                                                                                                                                 tGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATcc                      >  1:1033291/1‑150 (MQ=255)
                                                                                                                                   gcgcCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCctc                    <  2:1046033/150‑1 (MQ=255)
                                                                                                                                    cgcCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGACACTGAATCctct                   >  2:188369/1‑150 (MQ=255)
                                                                                                                                    cgcCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCACAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCctct                   >  1:1519179/1‑150 (MQ=255)
                                                                                                                                      tgatcGTACTGGTAATCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTAt                 >  1:1316305/5‑150 (MQ=255)
                                                                                                                                       gatcGTACTGGTAATCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAaacta                                                 <  1:849175/114‑1 (MQ=255)
                                                                                                                                       gatcGTACTGGTAATCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAaacta                                                 <  1:1331064/114‑1 (MQ=255)
                                                                                                                                       gatcGTACTGGTAATCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTAtt                >  2:69253/4‑150 (MQ=255)
                                                                                                                                          cGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTc             <  2:533864/150‑1 (MQ=255)
                                                                                                                                           gTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCg            <  2:1298413/150‑1 (MQ=255)
                                                                                                                                            tACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGa           >  1:981762/1‑150 (MQ=255)
                                                                                                                                            tACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGa           >  1:1116821/1‑150 (MQ=255)
                                                                                                                                             aCTGATTACCAGTACGATCTGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGAc          >  2:967062/1‑150 (MQ=255)
                                                                                                                                               tGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGActc        <  2:1277136/150‑1 (MQ=255)
                                                                                                                                               tGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGActc        >  1:805721/1‑150 (MQ=255)
                                                                                                                                                  ttACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCttt     >  2:376060/1‑150 (MQ=255)
                                                                                                                                                    aCCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTcc   >  1:807329/1‑150 (MQ=255)
                                                                                                                                                     ccATTACGATCAGTCATTTAACAATGTCAAAAGCCAGGCGACCAACCTGTATTTTCCGCGTTTTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCa  <  1:1686033/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTTTGGAATCATGGTATTGCTGGGGCATTTCTTGACTACAATTTATATGCCTCTCATTATGCACCACATCAGGGCGATAATTCGCAAAATATAAGTTCCTATGGGCAGGCTGGGGTTAATCTTGGGGCCTGGCGCCTGCGTACTGATTACCAGTACGATCAGTCATTTAACAATGGCAAAAGCCAGGCGACCAACCTGGATTTTCCGCGTATTTATTTGTTTCGCCCAATCCCAGCAATGAATGCAAAACTAACTATAGGTCAATACGATACTGAATCCTCTATTTTCGACTCTTTCCA  >  NC_000913/3187844‑3188142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: