Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,208,201 G→A 5.0% V60M (GTG→ATG)  ttdT → L‑tartrate/succinate antiporte

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,208,2010GA 5.0% 323.7 / 3.1 99V60M (GTG→ATG) ttdTL‑tartrate/succinate antiporte
Reads supporting (aligned to +/- strand):  ref base G (44/50);  new base A (3/2);  total (47/52)
Fisher's exact test for biased strand distribution p-value = 6.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 103 total aligned reads displayed.

ATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCC  >  NC_000913/3208052‑3208350
                                                                                                                                                     |                                                                                                                                                     
atCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGAtgg                                                                                                                                                       >  2:570036/1‑150 (MQ=255)
   ttGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGAtggtgg                                                                                                                                                    <  2:484614/150‑1 (MQ=255)
    tGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCCGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTggg                                                                                                                                                   <  1:933046/150‑1 (MQ=255)
    tGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTggg                                                                                                                                                   <  2:1720579/150‑1 (MQ=255)
     gcgcCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGt                                                                                                                                                  <  1:1456275/150‑1 (MQ=255)
     gcgcCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGt                                                                                                                                                  >  2:1345622/1‑150 (MQ=255)
     gcaataatGGCGATGACCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGt                                                                                                                                                  >  1:13112/8‑150 (MQ=255)
     gcaataatGGCGATGACCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGt                                                                                                                                                  >  1:358611/8‑150 (MQ=255)
      cgccgcTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGta                                                                                                                                                 <  2:668054/150‑1 (MQ=255)
      cgccgcTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGta                                                                                                                                                 <  1:1322568/150‑1 (MQ=255)
      cgccgcTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGta                                                                                                                                                 >  1:1395064/1‑150 (MQ=255)
      cgccgcTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTGCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGta                                                                                                                                                 <  1:662582/150‑1 (MQ=255)
       gccgcTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGtat                                                                                                                                                >  2:1215914/1‑150 (MQ=255)
        ccgcTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGtata                                                                                                                                               <  1:1706756/150‑1 (MQ=255)
             ggCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTGTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACGGATCCTCGCACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCcaa                                                                                                                                          >  1:185095/1‑149 (MQ=255)
              gCGGTCATCGCGATTATTGCTCTACTTCCGGTTCCAGCAGGGCTCGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGAACGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCcatc                                                                                                                                         >  2:303603/1‑150 (MQ=255)
              gCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCcatc                                                                                                                                         <  1:1010144/150‑1 (MQ=255)
                 gTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCatcatc                                                                                                                                      >  2:1287755/1‑150 (MQ=255)
                  tCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTTGTGGCGATGATGGATATACCCACCATCg                                                                                                                                     <  1:1389150/150‑1 (MQ=255)
                    aTCGCCATTATTGCTCTACTTCAGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGAGCGCTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGATGGATATACCCACCATCGcc                                                                                                                                   <  1:828755/150‑1 (MQ=255)
                     tCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCa                                                                                                                                  <  1:337867/150‑1 (MQ=255)
                      cGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCAt                                                                                                                                 <  2:1395064/150‑1 (MQ=255)
                      cGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCACCAt                                                                                                                                 <  2:476350/150‑1 (MQ=255)
                       gCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATa                                                                                                                                >  2:642804/1‑150 (MQ=255)
                       gCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGATTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGAGGGTATATCCATCATCGCCATa                                                                                                                                >  2:647392/1‑150 (MQ=255)
                         cATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATAct                                                                                                                              <  2:75869/150‑1 (MQ=255)
                          attattGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCGCTCCTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATActc                                                                                                                             <  1:309692/150‑1 (MQ=255)
                              ttGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACtctctc                                                                                                                         <  2:928450/150‑1 (MQ=255)
                                 ctctACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCt                                                                                                                      <  2:826072/150‑1 (MQ=255)
                                 ctctACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCt                                                                                                                      >  1:672716/1‑150 (MQ=255)
                                           ggTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGtt                                                                                                            >  1:152227/1‑150 (MQ=255)
                                            gTTCCCGCAGGGCTGGAGAACCATTCCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGtta                                                                                                           >  1:496791/1‑149 (MQ=255)
                                              tCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAg                                                                                                         >  2:791684/1‑150 (MQ=255)
                                               cccGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTAATGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCCATGGTGGGTATATACATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGc                                                                                                        >  1:747222/1‑150 (MQ=255)
                                                ccGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGcc                                                                                                       >  2:947022/1‑150 (MQ=255)
                                                ccGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGATGTTCAGcc                                                                                                       >  2:1308344/1‑150 (MQ=255)
                                                ccGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGATGGACTGATCCTCTATCCCGTGCCGGGTGCCGTGTTGGCGATGGTGGGTATATACATAATCTCCATACTCTCTCACTGGATGCTGTTAAGcc                                                                                                       >  2:68410/1‑150 (MQ=255)
                                                 cGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGCACCTTGGACTGATCCTAGAACCCCTGCCGCGTGCCGTGGTGGCGATGGTGGGTATAGCCAAAATCGCCAAACTCACACCCTGGCAGCGGTTCAGccc                                                                                                      >  1:1558786/1‑150 (MQ=255)
                                                 cGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGGGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGccc                                                                                                      >  1:807734/1‑150 (MQ=255)
                                                   cAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCgg                                                                                                    <  1:570036/150‑1 (MQ=255)
                                                   cAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGGGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCgg                                                                                                    <  2:931357/150‑1 (MQ=255)
                                                      ggCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGagc                                                                                                 <  1:1345622/150‑1 (MQ=255)
                                                       gCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTCGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCCGCCCGGagct                                                                                                >  1:221091/1‑149 (MQ=255)
                                                         tGGAGAACCATACCTGGCTCTACTTTGCAGTTATTACTGGCGTGATCGTTGGACTGATAATCGAACCCGTGCCCGGTGCCGTGGTGGCGATGGGGGGTATATCCATCATAGCAATTCTCTCACCCTGGCTGATTATCAGAACCGagcagc                                                                                              >  2:639175/1‑150 (MQ=255)
                                                          ggAGAACCATACCTGGCTCTACTTTGCCGTTTTTCCTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAgct                                                                                             <  1:290533/150‑1 (MQ=255)
                                                          ggAGAACCATACCTGGCGCTACTTAGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAgct                                                                                             <  2:1131100/150‑1 (MQ=255)
                                                            agaACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAgctcg                                                                                           <  1:1708133/150‑1 (MQ=255)
                                                             gaACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAgctcgc                                                                                          >  2:1715089/1‑150 (MQ=255)
                                                                  aTACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGc                                                                                     >  2:1221778/1‑150 (MQ=255)
                                                                   tACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGcc                                                                                    >  1:28898/1‑150 (MQ=255)
                                                                      cTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCCTACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAgg                                                                                 >  2:929874/1‑150 (MQ=255)
                                                                         gCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCtt                                                                              >  1:1040885/1‑150 (MQ=255)
                                                                         gCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCAGTGGTGGCGATGGTGGGTATATACATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCtt                                                                              >  1:1055087/1‑150 (MQ=255)
                                                                          ctctACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCttt                                                                             >  1:1180787/1‑150 (MQ=255)
                                                                          ctctACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCttt                                                                             >  2:640875/1‑150 (MQ=255)
                                                                             tACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTTCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTaaa                                                                          <  1:870303/150‑1 (MQ=255)
                                                                              aCTTTGCCGTTTTTACTGGCGTGACCGCTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAAt                                                                         <  2:1465559/150‑1 (MQ=255)
                                                                               cTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAAtt                                                                        >  1:1088625/1‑150 (MQ=255)
                                                                               cTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAAtt                                                                        >  1:1566858/1‑150 (MQ=255)
                                                                                 ttGCCGGTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCa                                                                      <  1:1646923/150‑1 (MQ=255)
                                                                                  tGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCAc                                                                     <  1:947022/150‑1 (MQ=255)
                                                                                    ccGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTg                                                                   <  2:56318/150‑1 (MQ=255)
                                                                                    ccGTTTTTACTGGCGGGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTg                                                                   <  1:243537/150‑1 (MQ=255)
                                                                                           tACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTTTATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATc                                                            <  2:218005/150‑1 (MQ=255)
                                                                                           tACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATc                                                            >  2:173140/1‑150 (MQ=255)
                                                                                             cTGGCGTGATCGTTTGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATccc                                                          <  2:398492/150‑1 (MQ=255)
                                                                                                 cGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCtctc                                                      <  2:450301/150‑1 (MQ=255)
                                                                                                  gTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCg                                                     >  1:69470/1‑150 (MQ=255)
                                                                                                  gTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGATCAGCCCGGAGCAGCTCGCTCATCCAGGCTTTAAATTCACTGCAAAATCCCTCTCg                                                     <  1:303603/150‑1 (MQ=255)
                                                                                                    gATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTg                                                   <  2:1476603/150‑1 (MQ=255)
                                                                                                      tCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTggg                                                 <  2:1088625/150‑1 (MQ=255)
                                                                                                         ttGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCg                                              <  2:672716/150‑1 (MQ=255)
                                                                                                              cTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTcc                                         <  2:425158/150‑1 (MQ=255)
                                                                                                              cTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCAGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTcc                                         >  1:853676/1‑150 (MQ=255)
                                                                                                                gATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCgg                                       >  1:783131/1‑150 (MQ=255)
                                                                                                                gATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCgg                                       >  1:1272985/1‑150 (MQ=255)
                                                                                                                    cTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGtttt                                   <  1:1287755/150‑1 (MQ=255)
                                                                                                                      cGAACCCGTGCCGTGTGCCTTGGTGGCGATGGTGGGTATATCAATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGTCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGtttttg                                 <  1:68410/150‑2 (MQ=255)
                                                                                                                      cGAACCCATGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTc                                 <  1:944928/150‑1 (MQ=255)
                                                                                                                       gAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAATATCCCTCTCGTGGGCCGTTTCCGGTTTTTCt                                <  1:647392/150‑1 (MQ=255)
                                                                                                                       gAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCt                                <  1:173140/150‑1 (MQ=255)
                                                                                                                        aaCCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTa                               <  2:358611/150‑1 (MQ=255)
                                                                                                                        aaCCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTa                               <  2:1180787/150‑1 (MQ=255)
                                                                                                                        aaCCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTa                               <  2:13112/150‑1 (MQ=255)
                                                                                                                        aaCCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTa                               >  2:474137/1‑150 (MQ=255)
                                                                                                                         aCCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTaa                              <  1:1191545/150‑1 (MQ=255)
                                                                                                                              tGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCgg                         <  2:1566858/150‑1 (MQ=255)
                                                                                                                                      gCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTACTTCGGTAATCTgg                 >  2:1142645/1‑150 (MQ=255)
                                                                                                                                       ccGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGc                >  1:992913/1‑150 (MQ=255)
                                                                                                                                         gtggtgGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTg              >  2:1407455/1‑150 (MQ=255)
                                                                                                                                          gtatggCGATGATGGATATACCCACCATCGCCATACTCTCTCCCTGGATGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGGTTTAAATTCACTGCAAAATCCCTCACGTGGGCCGATTCCGGTGTTTCTAATTCGGTTATCTGGCTGa             >  1:1107589/4‑150 (MQ=255)
                                                                                                                                             tggCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGAttt          <  1:1715089/150‑1 (MQ=255)
                                                                                                                                             tggCGATGATGGATATACCCACCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCCGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGAttt          >  2:177635/1‑150 (MQ=255)
                                                                                                                                             tggCGATGATGGATATACCCACCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGAttt          >  2:928860/1‑150 (MQ=255)
                                                                                                                                                cGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTcg       >  1:1321846/1‑150 (MQ=255)
                                                                                                                                                cGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTcg       <  1:1221778/150‑1 (MQ=255)
                                                                                                                                                cGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTcg       <  1:1519927/150‑1 (MQ=255)
                                                                                                                                                cGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTcg       >  2:57763/1‑150 (MQ=255)
                                                                                                                                                  aTGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCATCCAGGCTTTAAATTCACTGCAAAATCCCTGTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGGTATCTGGCTGATTTTcgcc     <  2:152227/150‑1 (MQ=255)
                                                                                                                                                     gtggGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCgccgcc  <  1:1131293/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATCTTGCGCCGCTGGCGGTCATCGCCATTATTGCTCTACTTCCGGTTCCCGCAGGGCTGGAGAACCATACCTGGCTCTACTTTGCCGTTTTTACTGGCGTGATCGTTGGACTGATCCTCGAACCCGTGCCGGGTGCCGTGGTGGCGATGGTGGGTATATCCATCATCGCCATACTCTCTCCCTGGCTGCTGTTCAGCCCGGAGCAGCTCGCTCAGCCAGGCTTTAAATTCACTGCAAAATCCCTCTCGTGGGCCGTTTCCGGTTTTTCTAATTCGGTTATCTGGCTGATTTTCGCCGCC  >  NC_000913/3208052‑3208350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: