Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,215,694 G→T 5.8% intergenic (+21/+33) ileX → / ← yqjH tRNA‑Ile/putative siderophore interacting protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,215,6940GT 5.8% 357.8 / 8.0 115intergenic (+21/+33)ileX/yqjHtRNA‑Ile/putative siderophore interacting protein
Reads supporting (aligned to +/- strand):  ref base G (52/56);  new base T (5/2);  total (57/58)
Fisher's exact test for biased strand distribution p-value = 2.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01

Only 100 of 119 total aligned reads displayed.

CGCGCGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAGT  >  NC_000913/3215545‑3215842
                                                                                                                                                     |                                                                                                                                                    
cgcgcgGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACgag                                                                                                                                                      >  2:916429/1‑150 (MQ=255)
    cgGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCCCCAGATATAGCAAAGGCTGACGAGaaaa                                                                                                                                                  >  1:633181/1‑149 (MQ=255)
    cgGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAAt                                                                                                                                                  >  2:373997/1‑150 (MQ=255)
      gTAATGGCTGGATTGCGACATGGAGTTACTTTATAATCAGCTACCATGGCCACTTAGCTCAGTGGTTTGAGCAGGCGACTCAAAATCGCTAGGTCTCTTGTTCAAGTCCATCAGTGGACACCAGATATAGCAACGGCTGACGAGAAATCg                                                                                                                                                >  2:1077622/1‑150 (MQ=255)
      gTAATGGCTGGATTGCGACACGGAGTTACTTAATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCg                                                                                                                                                <  1:613299/150‑1 (MQ=255)
        aaTGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTc                                                                                                                                              >  1:1472084/1‑150 (MQ=255)
         aTGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCa                                                                                                                                             <  1:1717610/150‑1 (MQ=255)
           ggCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGATTTCTCGTCAGc                                                                                                                                           <  1:1257585/150‑1 (MQ=255)
                 aTTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGATTTCTCGTCAGCctttgc                                                                                                                                     <  2:365791/150‑3 (MQ=255)
                 aTTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCcttttt                                                                                                                                     >  2:1391077/1‑150 (MQ=255)
                 aTTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGGTAGAGCAGGCGACTCATAATCGCTTGGGCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCcttttt                                                                                                                                     <  2:322927/150‑1 (MQ=255)
                  ttGCGACACGGAGTTCCTTTATATTCCGCTACCGTGGCCCCTTAGCTCTGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTGCAAGTCCAGCTGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCctttttc                                                                                                                                    <  1:454599/150‑1 (MQ=37)
                  ttGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCGGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCctttttc                                                                                                                                    <  1:1098549/150‑1 (MQ=255)
                   tGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCctttttct                                                                                                                                   >  2:748943/1‑150 (MQ=255)
                      gACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCctttttctttt                                                                                                                                >  2:1165029/1‑150 (MQ=255)
                      gACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCctttttctttt                                                                                                                                <  2:1213574/150‑1 (MQ=255)
                      gACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCctttttctttt                                                                                                                                >  2:19342/1‑150 (MQ=255)
                        cacGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTta                                                                                                                              <  1:675723/150‑1 (MQ=255)
                        cacGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTta                                                                                                                              <  2:1182099/150‑1 (MQ=255)
                         acGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGGCAGCCTTTTTCTTTTtat                                                                                                                             <  1:1359160/150‑1 (MQ=255)
                          cGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTtata                                                                                                                            <  1:1358452/150‑1 (MQ=255)
                          cGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTtata                                                                                                                            <  1:1259426/150‑1 (MQ=255)
                          cGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTtata                                                                                                                            >  2:769655/1‑150 (MQ=255)
                              gTTACTTTATAATCTGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATc                                                                                                                        <  1:770255/150‑1 (MQ=255)
                                tACTTTATAATCCGCTCCCATGGCCCCTTAGATCAGTGGCTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAg                                                                                                                      <  1:870425/150‑1 (MQ=255)
                                 aCTTTAAAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGt                                                                                                                     <  1:1703026/150‑1 (MQ=255)
                                  cTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGtt                                                                                                                    >  1:1595437/1‑150 (MQ=255)
                                   tttATAATCCGGTTCCATGGCCCCTTAGCTCAGTGTTTATAGCAGGCGACTCATTATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATATCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGttt                                                                                                                   <  2:170773/150‑2 (MQ=37)
                                   tttATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGAAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATTTAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTACTTTTTATATAACAGTTa                                                                                                                   >  2:1411048/1‑150 (MQ=255)
                                    ttgtaATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTAc                                                                                                                  <  1:640075/147‑1 (MQ=255)
                                    ttATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTAc                                                                                                                  >  1:904609/1‑150 (MQ=255)
                                      ataATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACtt                                                                                                                >  1:1565527/1‑150 (MQ=255)
                                       taATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCAGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACttt                                                                                                               >  1:234912/1‑150 (MQ=255)
                                        aaTCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTg                                                                                                              <  2:1027612/150‑1 (MQ=255)
                                         aTCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCctttttcttt                                                                                                                                 >  2:773109/1‑130 (MQ=255)
                                         aTCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCctttttcttt                                                                                                                                 <  1:773109/130‑1 (MQ=255)
                                          tCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCg                                                                                                            <  1:785833/150‑1 (MQ=255)
                                          tCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCg                                                                                                            <  2:403351/150‑1 (MQ=255)
                                               tACCTTGTCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATATTCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTATCTTTTTATATATCAGTTACTTTGCGTGCCa                                                                                                       <  1:80815/150‑1 (MQ=255)
                                                 ccATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAATGCTGACGAGAAATCGTCAGCATTGTTATTTTTATATATCAGTTTCTTTTCTTGCCAGt                                                                                                     >  2:1551777/1‑150 (MQ=25)
                                                     ggCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGc                                                                                                 <  1:603591/150‑1 (MQ=255)
                                                      gCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGcc                                                                                                <  2:1586321/150‑1 (MQ=255)
                                                      gCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCATGGTCGATGGTTAAATTCCAGCAGGGGCAACATGATATAGCAAAGGCTCCCGAGAAATCGTCAGCCTTTTTCTTTTTTGTTAGCAGTTAGTTTGGGTGCCATTAAGcc                                                                                                >  2:815352/1‑150 (MQ=21)
                                                       ccccTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAACTCGTCAGCCTTTTTCTTTTTATATTTCAGTTACTTTGCGTGCCAGTAAGCCg                                                                                               >  2:48287/1‑150 (MQ=255)
                                                        cccTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGGCAGCCTTTTTCATTTTATATATCAGTTACTTTGAGTGCCACTAAGCCGc                                                                                              >  1:1522957/1‑150 (MQ=255)
                                                               cTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTGTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGt                                                                                       >  2:1637467/1‑150 (MQ=255)
                                                                  aGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTAcc                                                                                    <  1:997094/150‑1 (MQ=255)
                                                                     ggTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCCCCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGc                                                                                 >  1:237915/1‑150 (MQ=255)
                                                                        tAGAGCAGGCGACTCATAATCGCTTGGTCGCTGTTTCAAGTCCAGCAGGGGCCAACAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTATTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTTCACGTACCCGCtgt                                                                              >  1:748520/1‑150 (MQ=255)
                                                                         agagCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCtgtg                                                                             <  1:1165029/150‑1 (MQ=255)
                                                                          gagCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTgg                                                                            >  2:583036/1‑150 (MQ=255)
                                                                           agCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTggg                                                                           <  1:583036/150‑1 (MQ=255)
                                                                            gCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAATTCCAGCAGGGGCCACCATATATATCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGt                                                                          <  2:170741/150‑1 (MQ=255)
                                                                            gCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGt                                                                          >  1:565814/1‑150 (MQ=255)
                                                                                gCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGACACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATa                                                                      <  2:904609/150‑1 (MQ=255)
                                                                                 cGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATAc                                                                     <  1:19342/150‑1 (MQ=255)
                                                                                   aCTCCTAATCGCCTGGGCGCTGGTTCATGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGGCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTCAGCCGCTGCACGTACCCGCTGTGGGTCATACTg                                                                   <  1:1062900/150‑1 (MQ=255)
                                                                                       aTAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTcc                                                               >  2:425408/1‑150 (MQ=255)
                                                                                          aTCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCt                                                            >  2:1489555/1‑150 (MQ=255)
                                                                                          aTCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCt                                                            <  2:234912/150‑1 (MQ=255)
                                                                                           tCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCCCCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCtt                                                           >  1:1064249/1‑150 (MQ=255)
                                                                                            cGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGTGTCATACTGTTCCGCTTc                                                          >  1:785625/1‑150 (MQ=255)
                                                                                            cGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTc                                                          <  1:373997/150‑1 (MQ=255)
                                                                                            cGCTTGGTCGCTGGTTCAAGTCCAGCAGGAGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTc                                                          >  1:11113/1‑150 (MQ=255)
                                                                                             gCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCa                                                         >  2:1004010/1‑150 (MQ=255)
                                                                                             gCTTGGTCGCTGGTTCAAGTCCAGCAGAGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCa                                                         >  2:358442/1‑150 (MQ=255)
                                                                                                  gTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAgcg                                                    >  2:338561/1‑150 (MQ=255)
                                                                                                   tCGCTGGTTAAAGTCCAGCATTGGCCACCAGATATAGCAAAGGCTGAAGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAgcgg                                                   <  2:748520/150‑1 (MQ=255)
                                                                                                    cGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAgcggc                                                  <  1:861193/150‑1 (MQ=255)
                                                                                                      cTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGcggcgg                                                <  1:961555/150‑1 (MQ=255)
                                                                                                        ggTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCt                                              >  2:180225/1‑150 (MQ=255)
                                                                                                        ggTCCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCt                                              <  1:378599/150‑1 (MQ=255)
                                                                                                         gTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCtt                                             <  2:1178036/150‑1 (MQ=255)
                                                                                                          ttCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGGCCGCCGGGTTCTTGTTATATTTCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTAATACTGTTCCGCTTCAAAGCGGCGGCttt                                            >  2:108241/1‑149 (MQ=255)
                                                                                                               gTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTc                                       >  2:354682/1‑150 (MQ=255)
                                                                                                                 ccAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCtt                                     <  1:544149/150‑1 (MQ=255)
                                                                                                                    tcagGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAAc                                  <  2:1565527/149‑1 (MQ=255)
                                                                                                                    gcagGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGcggcgg                                                <  1:834410/136‑1 (MQ=255)
                                                                                                                    gcagGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGcggcgg                                                >  2:834410/1‑136 (MQ=255)
                                                                                                                    gcagGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAAc                                  >  2:463909/1‑150 (MQ=255)
                                                                                                                       ggggCCACCTGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGAc                               <  2:350459/150‑1 (MQ=255)
                                                                                                                         ggCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACtt                             >  2:1635512/1‑150 (MQ=255)
                                                                                                                                   aataACAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGcc                   <  1:507633/149‑1 (MQ=255)
                                                                                                                                    aagaaaaaGGCTGACGATTTCTCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGTCAGTAAGCCGCTGCACGTACCCGATGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGcag                  >  2:1314006/6‑148 (MQ=255)
                                                                                                                                    aagaaaaaGGCTGACGATTTCTCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCg                  >  1:730987/6‑150 (MQ=255)
                                                                                                                                     taGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCt                        <  2:700619/143‑1 (MQ=255)
                                                                                                                                      gaaaaaGGCTGACGATTTCTCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGt                >  2:1530027/4‑150 (MQ=255)
                                                                                                                                      aGCAAAGGCTGACGAGAAATCTTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGt                <  1:1411048/150‑1 (MQ=255)
                                                                                                                                      aGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGt                <  2:343611/150‑1 (MQ=255)
                                                                                                                                      aGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGt                <  1:655004/150‑1 (MQ=255)
                                                                                                                                           aGGCTGACGATTTCTCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCTAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATcc           >  2:936379/1‑150 (MQ=255)
                                                                                                                                           aGGCTGACGATTTCTCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATcc           >  1:313734/1‑150 (MQ=255)
                                                                                                                                            gtcttaCGAGAAATAGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGATCCAGTAAGCCGCTGCACGTACCCGCTGTGGGACATACTGTTCCTCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCa          <  1:1077622/145‑1 (MQ=255)
                                                                                                                                             gCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAg         <  2:1472084/150‑1 (MQ=255)
                                                                                                                                               tGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGAt       >  2:1255323/1‑150 (MQ=255)
                                                                                                                                               tGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGATTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGAt       >  1:1202296/1‑150 (MQ=255)
                                                                                                                                                 aCGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGa     <  2:565814/150‑1 (MQ=255)
                                                                                                                                                   gagaAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAg   >  1:488680/1‑150 (MQ=255)
                                                                                                                                                   gagaAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAg   >  1:448383/1‑150 (MQ=255)
                                                                                                                                                    agaAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAGt  >  2:1576455/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CGCGCGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAGT  >  NC_000913/3215545‑3215842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: