Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,215,696 A→T 6.0% intergenic (+23/+31) ileX → / ← yqjH tRNA‑Ile/putative siderophore interacting protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,215,6960AT 6.0% 340.7 / 8.0 117intergenic (+23/+31)ileX/yqjHtRNA‑Ile/putative siderophore interacting protein
Reads supporting (aligned to +/- strand):  ref base A (54/56);  new base T (5/2);  total (59/58)
Fisher's exact test for biased strand distribution p-value = 4.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.01e-01

Only 100 of 119 total aligned reads displayed.

CGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAGTAA  >  NC_000913/3215549‑3215844
                                                                                                                                                   |                                                                                                                                                    
cgGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCCCCAGATATAGCAAAGGCTGACGAGaaaa                                                                                                                                                    >  1:633181/1‑149 (MQ=255)
cgGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAAt                                                                                                                                                    >  2:373997/1‑150 (MQ=255)
  gTAATGGCTGGATTGCGACACGGAGTTACTTAATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCg                                                                                                                                                  <  1:613299/150‑1 (MQ=255)
    aaTGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTc                                                                                                                                                >  1:1472084/1‑150 (MQ=255)
     aTGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCa                                                                                                                                               <  1:1717610/150‑1 (MQ=255)
       ggCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGATTTCTCGTCAGc                                                                                                                                             <  1:1257585/150‑1 (MQ=255)
             aTTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGATTTCTCGTCAGCctttgc                                                                                                                                       <  2:365791/150‑3 (MQ=255)
             aTTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCcttttt                                                                                                                                       >  2:1391077/1‑150 (MQ=255)
             aTTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGGTAGAGCAGGCGACTCATAATCGCTTGGGCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCcttttt                                                                                                                                       <  2:322927/150‑1 (MQ=255)
              ttGCGACACGGAGTTCCTTTATATTCCGCTACCGTGGCCCCTTAGCTCTGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTGCAAGTCCAGCTGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCctttttc                                                                                                                                      <  1:454599/150‑1 (MQ=37)
              ttGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCGGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCctttttc                                                                                                                                      <  1:1098549/150‑1 (MQ=255)
               tGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCctttttct                                                                                                                                     >  2:748943/1‑150 (MQ=255)
                  gACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCctttttctttt                                                                                                                                  <  2:1219630/150‑1 (MQ=255)
                  gACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCctttttctttt                                                                                                                                  >  2:19342/1‑150 (MQ=255)
                  gACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCctttttctttt                                                                                                                                  >  2:1165029/1‑150 (MQ=255)
                    cacGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTta                                                                                                                                <  2:1182099/150‑1 (MQ=255)
                      cGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTtata                                                                                                                              <  1:1358452/150‑1 (MQ=255)
                      cGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTtata                                                                                                                              <  1:1259426/150‑1 (MQ=255)
                      cGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTtata                                                                                                                              <  1:1674698/150‑1 (MQ=255)
                      cGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTtata                                                                                                                              >  2:769655/1‑150 (MQ=255)
                          gTTACTTTATAATCTGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATc                                                                                                                          <  1:770255/150‑1 (MQ=255)
                            tACTTTATAATCCGCTCCCATGGCCCCTTAGATCAGTGGCTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAg                                                                                                                        <  1:870425/150‑1 (MQ=255)
                             aCTTTAAAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGt                                                                                                                       <  1:1703026/150‑1 (MQ=255)
                               tttATAATCCGGTTCCATGGCCCCTTAGCTCAGTGTTTATAGCAGGCGACTCATTATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATATCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGttt                                                                                                                     <  2:170773/150‑2 (MQ=37)
                               tttATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGCCTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTa                                                                                                                     >  1:806642/1‑150 (MQ=255)
                               tttATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGAAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATTTAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTACTTTTTATATAACAGTTa                                                                                                                     >  2:1411048/1‑150 (MQ=255)
                                ttgtaATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTAc                                                                                                                    <  1:640075/147‑1 (MQ=255)
                                  ataATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACtt                                                                                                                  >  1:1565527/1‑150 (MQ=255)
                                   taATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCAGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACttt                                                                                                                 >  1:234912/1‑150 (MQ=255)
                                    aaTCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTg                                                                                                                <  2:1027612/150‑1 (MQ=255)
                                     aTCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCctttttcttt                                                                                                                                   <  1:773109/130‑1 (MQ=255)
                                           tACCTTGTCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATATTCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTATCTTTTTATATATCAGTTACTTTGCGTGCCa                                                                                                         <  1:80815/150‑1 (MQ=255)
                                             ccATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAATGCTGACGAGAAATCGTCAGCATTGTTATTTTTATATATCAGTTTCTTTTCTTGCCAGt                                                                                                       >  2:1551777/1‑150 (MQ=25)
                                                tGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAg                                                                                                    >  2:3450/1‑150 (MQ=255)
                                                  gCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCATGGTCGATGGTTAAATTCCAGCAGGGGCAACATGATATAGCAAAGGCTCCCGAGAAATCGTCAGCCTTTTTCTTTTTTGTTAGCAGTTAGTTTGGGTGCCATTAAGcc                                                                                                  >  2:815352/1‑150 (MQ=21)
                                                   ccccTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAACTCGTCAGCCTTTTTCTTTTTATATTTCAGTTACTTTGCGTGCCAGTAAGCCg                                                                                                 >  2:48287/1‑150 (MQ=255)
                                                    cccTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGGCAGCCTTTTTCATTTTATATATCAGTTACTTTGAGTGCCACTAAGCCGc                                                                                                >  1:1522957/1‑150 (MQ=255)
                                                           cTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTGTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGt                                                                                         >  2:1637467/1‑150 (MQ=255)
                                                              aGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTAcc                                                                                      <  1:997094/150‑1 (MQ=255)
                                                                 ggTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCCCCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGc                                                                                   >  1:237915/1‑150 (MQ=255)
                                                                    tAGAGCAGGCGACTCATAATCGCTTGGTCGCTGTTTCAAGTCCAGCAGGGGCCAACAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTATTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTTCACGTACCCGCtgt                                                                                >  1:748520/1‑150 (MQ=255)
                                                                     agagCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCtgtg                                                                               <  1:1165029/150‑1 (MQ=255)
                                                                       agCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTggg                                                                             <  1:583036/150‑1 (MQ=255)
                                                                        gCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAATTCCAGCAGGGGCCACCATATATATCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGt                                                                            <  2:170741/150‑1 (MQ=255)
                                                                        gCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGt                                                                            >  1:565814/1‑150 (MQ=255)
                                                                            gCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGACACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATa                                                                        <  2:904609/150‑1 (MQ=255)
                                                                             cGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATAc                                                                       <  1:19342/150‑1 (MQ=255)
                                                                               aCTCCTAATCGCCTGGGCGCTGGTTCATGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGGCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTCAGCCGCTGCACGTACCCGCTGTGGGTCATACTg                                                                     <  1:1062900/150‑1 (MQ=255)
                                                                                   aTAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTcc                                                                 >  2:425408/1‑150 (MQ=255)
                                                                                      aTCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCt                                                              <  2:234912/150‑1 (MQ=255)
                                                                                       tCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCCCCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCtt                                                             >  1:1064249/1‑150 (MQ=255)
                                                                                        cGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGTGTCATACTGTTCCGCTTc                                                            >  1:785625/1‑150 (MQ=255)
                                                                                        cGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTc                                                            >  2:1081674/1‑150 (MQ=255)
                                                                                        cGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTc                                                            <  1:373997/150‑1 (MQ=255)
                                                                                        cGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGGCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGAAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTc                                                            <  1:939982/150‑1 (MQ=255)
                                                                                        cGCTTGGTCGCTGGTTCAAGTCCAGCAGGAGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTc                                                            >  1:11113/1‑150 (MQ=255)
                                                                                         gCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCa                                                           >  2:1004010/1‑150 (MQ=255)
                                                                                         gCTTGGTCGCTGGTTCAAGTCCAGCAGAGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCa                                                           >  2:358442/1‑150 (MQ=255)
                                                                                              gTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAgcg                                                      >  2:338561/1‑150 (MQ=255)
                                                                                              gTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAgcg                                                      >  2:1614828/1‑150 (MQ=255)
                                                                                               tCGCTGGTTAAAGTCCAGCATTGGCCACCAGATATAGCAAAGGCTGAAGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAgcgg                                                     <  2:748520/150‑1 (MQ=255)
                                                                                                cGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAgcggc                                                    <  1:861193/150‑1 (MQ=255)
                                                                                                cGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAgcggc                                                    <  2:775524/150‑1 (MQ=255)
                                                                                                  cTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGcggcgg                                                  <  1:961555/150‑1 (MQ=255)
                                                                                                    ggTCCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCt                                                <  1:378599/150‑1 (MQ=255)
                                                                                                     gTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCtt                                               <  1:1391077/150‑1 (MQ=255)
                                                                                                     gTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCtt                                               <  2:1178036/150‑1 (MQ=255)
                                                                                                      ttCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGGCCGCCGGGTTCTTGTTATATTTCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTAATACTGTTCCGCTTCAAAGCGGCGGCttt                                              >  2:108241/1‑149 (MQ=255)
                                                                                                          aGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAAtt                                          >  2:1494090/1‑150 (MQ=255)
                                                                                                          aGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAAtt                                          >  1:506162/1‑150 (MQ=255)
                                                                                                           gTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTc                                         >  2:354682/1‑150 (MQ=255)
                                                                                                             ccAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCtt                                       <  1:544149/150‑1 (MQ=255)
                                                                                                                tcagGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAAc                                    <  2:1565527/149‑1 (MQ=255)
                                                                                                                gcagGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGcggcgg                                                  >  2:834410/1‑136 (MQ=255)
                                                                                                                gcagGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGcggcgg                                                  <  1:834410/136‑1 (MQ=255)
                                                                                                                gcagGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAAc                                    >  2:463909/1‑150 (MQ=255)
                                                                                                                  agGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGa                                  >  2:423307/1‑150 (MQ=255)
                                                                                                                   ggggCCACCTGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGAc                                 <  2:350459/150‑1 (MQ=255)
                                                                                                                     ggCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACtt                               >  2:1635512/1‑150 (MQ=255)
                                                                                                                       ccaccaGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTa                             <  2:617364/150‑1 (MQ=255)
                                                                                                                               aataACAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGcc                     <  1:507633/149‑1 (MQ=255)
                                                                                                                                aagaaaaaGGCTGACGATTTCTCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGTCAGTAAGCCGCTGCACGTACCCGATGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGcag                    >  2:1314006/6‑148 (MQ=255)
                                                                                                                                aagaaaaaGGCTGACGATTTCTCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCg                    >  1:730987/6‑150 (MQ=255)
                                                                                                                                 taGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCt                          >  1:700619/1‑143 (MQ=255)
                                                                                                                                 taGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCt                          <  2:700619/143‑1 (MQ=255)
                                                                                                                                  gaaaaaGGCTGACGATTTCTCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGt                  >  2:1530027/4‑150 (MQ=255)
                                                                                                                                  aGCAAAGGCTGACGAGAAATCTTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGt                  <  1:1411048/150‑1 (MQ=255)
                                                                                                                                  aGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGt                  <  1:655004/150‑1 (MQ=255)
                                                                                                                                   gCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGtt                 >  1:607593/1‑150 (MQ=255)
                                                                                                                                    cAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTa                <  1:338561/150‑1 (MQ=255)
                                                                                                                                       aGGCTGACGATTTCTCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCTAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATcc             >  2:936379/1‑150 (MQ=255)
                                                                                                                                        gtcttaCGAGAAATAGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGATCCAGTAAGCCGCTGCACGTACCCGCTGTGGGACATACTGTTCCTCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCa            <  1:1077622/145‑1 (MQ=255)
                                                                                                                                         gCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAg           <  2:1472084/150‑1 (MQ=255)
                                                                                                                                           tGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGAt         >  2:1255323/1‑150 (MQ=255)
                                                                                                                                           tGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGATTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGAt         >  1:1202296/1‑150 (MQ=255)
                                                                                                                                            gACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATg        >  2:190206/1‑150 (MQ=255)
                                                                                                                                             aCGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGa       <  2:565814/150‑1 (MQ=255)
                                                                                                                                               gagaAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAg     >  1:448383/1‑150 (MQ=255)
                                                                                                                                                agaAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAGt    >  2:1576455/1‑150 (MQ=255)
                                                                                                                                                  aaaTCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAGTaa  >  1:1192377/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CGGTAATGGCTGGATTGCGACACGGAGTTACTTTATAATCCGCTACCATGGCCCCTTAGCTCAGTGGTTAGAGCAGGCGACTCATAATCGCTTGGTCGCTGGTTCAAGTCCAGCAGGGGCCACCAGATATAGCAAAGGCTGACGAGAAATCGTCAGCCTTTTTCTTTTTATATATCAGTTACTTTGCGTGCCAGTAAGCCGCTGCACGTACCCGCTGTGGGTCATACTGTTCCGCTTCAAAGCGGCGGCTTAAATTCTTAACGACTTTACCTTCGCCGGTTATCCAGATGAAGTAA  >  NC_000913/3215549‑3215844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: