Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,221,150 A→T 5.4% I33N (ATC→AAC) ‡ ygjH ← putative tRNA binding protein; putative tRNA corner chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,221,1500AT 5.4% 326.1 / 5.9 111I33N (ATC→AAC) ‡ygjHputative tRNA binding protein; putative tRNA corner chaperone
Reads supporting (aligned to +/- strand):  ref base A (50/55);  new base T (3/3);  total (53/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.68e-01

Only 100 of 112 total aligned reads displayed.

GCATGCATTCCGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGTTTTTCT  >  NC_000913/3221001‑3221298
                                                                                                                                                     |                                                                                                                                                    
gcatgcatTCCGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGa                                                                                                                                                      >  2:703685/1‑150 (MQ=255)
 catgcatTCCGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCACCACATCCACCTGTACGAt                                                                                                                                                     >  1:1344128/1‑150 (MQ=255)
      atTCCGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACa                                                                                                                                                <  1:1091171/150‑1 (MQ=255)
      atTCCGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTGTGCAGCGTTTTTTGGCCCACATCAACCTGTACGATGTACa                                                                                                                                                >  2:1616780/1‑150 (MQ=255)
          ccaCGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCtt                                                                                                                                            >  1:1016389/3‑150 (MQ=255)
          cGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCtt                                                                                                                                            <  2:1135468/150‑1 (MQ=255)
            aCGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGt                                                                                                                                          <  2:608072/150‑1 (MQ=255)
             cGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACATCGTACAGGTTgat                                                                                                                                         <  1:1290277/150‑3 (MQ=255)
             cGTTTCACCACGCATTTTCGCTTTTAGCAGATTGCATAAGACCACCACCGTTTTCCGCATCAGCTCTTCTTCGCTGTTGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCTCATGATCCTGTACGATGTACAGCTTGTc                                                                                                                                         <  1:1625643/150‑1 (MQ=255)
              gTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACATCGTACAGGTTgatg                                                                                                                                        <  2:389446/150‑4 (MQ=255)
               tttCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCgg                                                                                                                                       <  1:178587/150‑1 (MQ=255)
                ttCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGc                                                                                                                                      >  1:1625383/1‑150 (MQ=255)
                  caccacGAATTTTCGCTATTTGCAGATTGCATAATACCAGCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAAGCTGTACATCGTACAGGTTgatgtggg                                                                                                                                    <  1:306452/150‑8 (MQ=255)
                     cacGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCTTCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGtttt                                                                                                                                 <  2:237938/150‑1 (MQ=255)
                         cATTTTCGCTTTTTGCAGATTTCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGGCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATg                                                                                                                             <  1:1014334/150‑1 (MQ=255)
                              tCGCTTTTTGCAGATTGCATAAGACCACCACCGTATTCCCCATCAGCTCTTCTTCGCTGTAGTACGTCACCAGGCTGGTCACGGTATGCAGCGTTTTTTGCCCCACATCAACAATTACGATTTACAGCTTGTCGGCGTTTTCATGGCGtt                                                                                                                        >  1:949702/1‑150 (MQ=255)
                               cGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTTTCGGCGTTTTCATGGCGttt                                                                                                                       >  2:169189/1‑150 (MQ=255)
                                 cTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGAGTTTCAc                                                                                                                    >  2:352545/1‑150 (MQ=255)
                                     ttGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCTCCAGGCGGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTc                                                                                                                 <  1:342802/150‑1 (MQ=255)
                                     ttGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTc                                                                                                                 <  1:1665354/150‑1 (MQ=255)
                                     ttGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTc                                                                                                                 <  1:555277/150‑1 (MQ=255)
                                       gCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCca                                                                                                               <  2:653848/150‑1 (MQ=255)
                                        cAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCcac                                                                                                              <  1:1572563/150‑1 (MQ=255)
                                        cAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCAGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATAAACCTGTAAGATGTACAGCTTGTCTGCGTTTTAATGGCGTTTAACTTCcaa                                                                                                              >  2:788593/1‑149 (MQ=255)
                                         aGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCcaca                                                                                                             <  1:529839/150‑1 (MQ=255)
                                          gATTGCATAAGACCACCACCGTTTTCCCCATCACCTATTATTCGCTGTAGTACGGCACCAGCCTGGTATCGGTTTGCAGCGTTTTTTGCCCCACATCAAACTGAACGATGTACAGCTTGTCGGCGTTTTAATGGCGTATCTCATCCACaa                                                                                                            >  1:95912/1‑150 (MQ=255)
                                            ttGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATc                                                                                                          >  1:1107439/1‑150 (MQ=255)
                                              gCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCtt                                                                                                        >  1:573991/1‑150 (MQ=255)
                                                aTAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTc                                                                                                      <  1:462401/150‑1 (MQ=255)
                                                aTAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTc                                                                                                      >  1:980060/1‑150 (MQ=255)
                                                 tAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTcc                                                                                                     <  1:970263/150‑1 (MQ=255)
                                                 tAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTcc                                                                                                     >  1:1256269/1‑150 (MQ=255)
                                                 tAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTcc                                                                                                     <  2:396609/150‑1 (MQ=255)
                                                  aaGAACAGCACCGTGATCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCg                                                                                                    <  2:308134/150‑1 (MQ=255)
                                                   aGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGa                                                                                                   >  2:1130082/1‑150 (MQ=255)
                                                    gACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGAc                                                                                                  >  2:1621932/1‑150 (MQ=255)
                                                       caccaccGTTTTCCCCTTCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGAcgcg                                                                                               <  2:299749/150‑1 (MQ=255)
                                                        accaccGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACCTCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATATTTCCGACgcgc                                                                                              >  2:1448949/1‑150 (MQ=255)
                                                         ccaccGTTTTCCCCATCAGCTCTTCTTTGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTCCCCCCCATCAACCTGTACGATGTACAGCTTGTCGGGGTTTTAATGGCGTTTCCCTTCAACAATCTTTCTCACGCTCa                                                                                             >  2:501784/1‑150 (MQ=255)
                                                         ccaccGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCa                                                                                             <  1:886502/150‑1 (MQ=255)
                                                          caccGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACCGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTACGACGCGCAt                                                                                            >  2:1323825/1‑150 (MQ=255)
                                                           accGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCAtt                                                                                           <  2:1016389/150‑1 (MQ=255)
                                                           accGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCAtt                                                                                           >  1:898639/1‑150 (MQ=255)
                                                           accGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCAtt                                                                                           <  1:728284/150‑1 (MQ=255)
                                                           accGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTATCAGAcgcggctt                                                                                           >  1:1041500/1‑146 (MQ=255)
                                                             cGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTATACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTc                                                                                         <  2:598710/150‑1 (MQ=255)
                                                              gTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCGACATCAACCTGTACGATGTACAGCTTGTCGTCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTcc                                                                                        <  2:280277/150‑1 (MQ=255)
                                                              gTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTcc                                                                                        >  2:1475931/1‑150 (MQ=255)
                                                               ttttCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAAACTGTACGATGTACAGCTTGTCTGAGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCa                                                                                       >  2:97148/1‑150 (MQ=255)
                                                                tttCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACTATGTACAGCTTGTGGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAg                                                                                      <  2:95912/150‑1 (MQ=255)
                                                                  tCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGAc                                                                                    <  2:938926/150‑1 (MQ=255)
                                                                   ccccATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACg                                                                                   >  1:1625335/1‑150 (MQ=255)
                                                                   ccccATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAAACTGTACGATGTACAGCTTGTAGGCGTTTTCATGGCGTTTCACTTCCAAAATCTTTCCGACGCGCATTTCCAGACg                                                                                   >  2:557656/1‑150 (MQ=255)
                                                                     ccATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTg                                                                                 <  1:1371326/150‑1 (MQ=255)
                                                                     ccATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTg                                                                                 <  1:1178603/150‑1 (MQ=255)
                                                                          aGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCAACAATCTTTCCGACGCGCATTTCCAGACGTTCaaaa                                                                            >  2:1058465/1‑150 (MQ=255)
                                                                              cttcttCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAg                                                                        <  2:1256269/150‑1 (MQ=255)
                                                                                tcttcGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTATGCCGCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCg                                                                      <  1:256665/150‑1 (MQ=255)
                                                                                 cttcGCTGTAGTACGGCATCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCGACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGt                                                                     >  2:285379/1‑150 (MQ=255)
                                                                                  ttcGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTa                                                                    >  1:565574/1‑150 (MQ=255)
                                                                                     gCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGc                                                                 >  2:1022954/1‑150 (MQ=255)
                                                                                               ggCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTcc                                                       <  2:1107439/150‑1 (MQ=255)
                                                                                                 cACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCAt                                                     <  1:1045184/150‑1 (MQ=255)
                                                                                                 cACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCAt                                                     <  1:1469908/150‑1 (MQ=255)
                                                                                                   ccAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCAGGGTTTCCATTg                                                   <  2:949702/150‑1 (MQ=255)
                                                                                                     aGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCt                                                 >  2:523010/1‑150 (MQ=255)
                                                                                                     aGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTAAGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCCCTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGAAAAATCAGCGTAAGCCAAGGTTTCCATTTCt                                                 >  2:1202158/1‑150 (MQ=255)
                                                                                                      ggCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCtt                                                <  2:898639/150‑1 (MQ=255)
                                                                                                      ggCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCtt                                                <  2:1625335/150‑1 (MQ=255)
                                                                                                        cTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTtcc                                              <  1:1689544/150‑1 (MQ=255)
                                                                                                        cTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCAACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGACACGGTTTCCATTGCTtcc                                              >  1:257591/1‑150 (MQ=255)
                                                                                                          ggTCACGGTTTGCAGCGTTTTTTGCCCCACATAAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTtcctc                                            >  2:884654/1‑150 (MQ=255)
                                                                                                           gTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTtcctcc                                           >  1:1333026/1‑150 (MQ=255)
                                                                                                                ggTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCTTAg                                      >  1:1685194/1‑150 (MQ=255)
                                                                                                                  tttGCAGCGTTTTTTTCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCTTAggg                                    <  1:1550375/150‑1 (MQ=255)
                                                                                                                  tttGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCTTAggg                                    <  1:703685/150‑1 (MQ=255)
                                                                                                                    tGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCTTAGGGTa                                  >  2:1439964/1‑150 (MQ=255)
                                                                                                                       aGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTc                                                                                                      >  1:522867/1‑79 (MQ=255)
                                                                                                                        gCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCTTAGGGTAAAAt                              <  2:1482616/150‑1 (MQ=255)
                                                                                                                          gTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCTTAGGGTAAAAttt                            >  1:222889/1‑150 (MQ=255)
                                                                                                                             ttttGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTAc                         <  2:1628983/150‑1 (MQ=255)
                                                                                                                               ttGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCTTTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTa                       <  1:1058465/150‑1 (MQ=255)
                                                                                                                               ttGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTa                       >  1:213194/1‑150 (MQ=255)
                                                                                                                                 gCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTaaa                     <  1:1130082/150‑1 (MQ=255)
                                                                                                                                 gCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACCaaa                     >  2:114741/1‑150 (MQ=255)
                                                                                                                                  ccccACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAAc                    <  2:1625383/150‑1 (MQ=255)
                                                                                                                                   cccACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACt                   >  1:638899/1‑150 (MQ=255)
                                                                                                                                   cccACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACt                   >  2:1122991/1‑150 (MQ=255)
                                                                                                                                      acaTCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACtata                >  1:443485/1‑150 (MQ=255)
                                                                                                                                       cgacAAGCTGTACATCGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAg               >  2:154310/4‑150 (MQ=255)
                                                                                                                                       caTCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAg               <  1:1705907/150‑1 (MQ=255)
                                                                                                                                       caTCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAg               <  2:1685194/150‑1 (MQ=255)
                                                                                                                                        gacAAGCTGTACATCGTACAGGTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCAAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTTCTCCCTTAGGGTAAAATTTTACTAAACTATAGc              >  2:928744/3‑150 (MQ=255)
                                                                                                                                         acAATCTGTACATCGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTACAGACGAGCAAAATCAGCGTAAGCCACGGTTTAAATTGCTTCCTCCATTAGGGTAAAATTTTACTAACCTATAGCa             >  1:280740/2‑150 (MQ=255)
                                                                                                                                             ccTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAg         >  2:1680383/1‑150 (MQ=255)
                                                                                                                                              cTGTACGATGTACAGCTTGTCGGAGTTTTCATGGAGTTTCACTTCCACAATCTTTCCGACGAGCATTTCCAGAAGTTCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGt        <  1:97148/150‑1 (MQ=255)
                                                                                                                                                gTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGttt      >  2:1418891/1‑150 (MQ=255)
                                                                                                                                                   cGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGTTTTtc   <  2:980060/150‑1 (MQ=255)
                                                                                                                                                   cGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGTTTTtc   <  2:565574/150‑1 (MQ=255)
                                                                                                                                                   cGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGTTTTtc   <  1:922821/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCATGCATTCCGACGTTTCACCACGCATTTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGTTTTTCT  >  NC_000913/3221001‑3221298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: