Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,238,465 G→T 5.4% intergenic (+168/‑115) ygjR → / → alx putative NAD(P)‑dependent dehydrogenase/putative membrane‑bound redox modulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,238,4650GT 5.4% 431.7 / 7.9 137intergenic (+168/‑115)ygjR/alxputative NAD(P)‑dependent dehydrogenase/putative membrane‑bound redox modulator
Reads supporting (aligned to +/- strand):  ref base G (58/71);  new base T (4/4);  total (62/75)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 139 total aligned reads displayed.

GTAAAAGGTATGTAACAGACCATTGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCG  >  NC_000913/3238316‑3238613
                                                                                                                                                     |                                                                                                                                                    
  aaaaGGTATGTAACAGACCATTGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGAc                                                                                                                                                    >  1:894870/1‑150 (MQ=255)
     aGGTATGTAACAGACCATTGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTa                                                                                                                                                 >  2:354068/1‑150 (MQ=255)
       gTATGTAACAGACCATTGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACTATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTAtt                                                                                                                                               <  1:454841/150‑1 (MQ=255)
         aTGTAACAGACCATTGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTcc                                                                                                                                             <  2:343354/150‑1 (MQ=255)
          tGTAACAGACCATTGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCt                                                                                                                                            <  2:1578746/150‑1 (MQ=255)
          tGTAACAGACCATTGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCt                                                                                                                                            <  2:1211922/150‑1 (MQ=255)
           gTAACAGACCATTGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGTCTGCGTATTCCtt                                                                                                                                           <  1:1024003/150‑1 (MQ=255)
           gTAACAGACCATTGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGTCTGCGTATTCCtt                                                                                                                                           <  1:1017621/150‑1 (MQ=255)
           gTAACAGACCATTGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCtt                                                                                                                                           <  2:674988/150‑1 (MQ=255)
            tAACAGACCATTGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGTCTGCGTATTCCttt                                                                                                                                          <  2:1363095/150‑1 (MQ=255)
            tAACAGACCATTGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCttt                                                                                                                                          >  2:989361/1‑150 (MQ=255)
            tAACAGACCATTGACTGGCTGAATGGTCTGTCATACATTGTTACCTGCAAAGGGGAGTAACTTCATTTCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCttt                                                                                                                                          >  2:1518573/1‑150 (MQ=255)
             aaCAGACCATTGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCtttt                                                                                                                                         >  2:1385877/1‑150 (MQ=255)
              aCAGACCATTGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCttttt                                                                                                                                        <  1:470839/150‑1 (MQ=255)
              aCAGACCATTGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCttttt                                                                                                                                        <  1:1174382/150‑1 (MQ=255)
              aCAGACCATTGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCttttt                                                                                                                                        <  2:661195/150‑1 (MQ=255)
                aGACCATTGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTttg                                                                                                                                      <  2:226289/150‑1 (MQ=255)
                   ccATTGACTGGCTGAATGGTGTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGTAATACGCAGACGTATTCCTTTTttgttg                                                                                                                                   <  2:1102327/150‑1 (MQ=255)
                    cATTGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGTCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTtgttgt                                                                                                                                  >  2:741051/1‑150 (MQ=255)
                    cATTGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTtgttgt                                                                                                                                  <  2:410142/150‑1 (MQ=255)
                    cATTGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTtgttgt                                                                                                                                  >  2:46863/1‑150 (MQ=255)
                    cATTGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTtgttgt                                                                                                                                  <  2:1114510/150‑1 (MQ=255)
                     aTTGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTa                                                                                                                                 <  1:217445/150‑1 (MQ=255)
                     aTTGACTGGATGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGCTCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTa                                                                                                                                 <  2:71225/150‑1 (MQ=255)
                        gACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGt                                                                                                                              >  2:449734/1‑150 (MQ=255)
                                gAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCtt                                                                                                                      <  2:73385/150‑1 (MQ=255)
                                  aTGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGc                                                                                                                    >  2:1038816/1‑150 (MQ=255)
                                  aTGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGc                                                                                                                    <  1:354068/150‑1 (MQ=255)
                                   tGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGcc                                                                                                                   <  2:769940/150‑1 (MQ=255)
                                   tGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTCGcc                                                                                                                   >  2:1140501/1‑150 (MQ=255)
                                    ggTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCg                                                                                                                  >  1:1420859/1‑150 (MQ=255)
                                        tGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAATGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAg                                                                                                              <  2:1622406/150‑1 (MQ=255)
                                        tGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAg                                                                                                              >  1:1141672/1‑150 (MQ=255)
                                         gTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAgg                                                                                                             <  1:186821/150‑1 (MQ=255)
                                           cATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAAAACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCg                                                                                                           <  2:1319869/150‑1 (MQ=255)
                                             tACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAg                                                                                                         >  2:1430629/1‑150 (MQ=255)
                                             tACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAg                                                                                                         <  1:1449947/150‑1 (MQ=255)
                                             tACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAg                                                                                                         >  1:1563029/1‑150 (MQ=255)
                                             tACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAg                                                                                                         <  1:989361/150‑1 (MQ=255)
                                               cTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGCGCAACCCGAAAGGAATACGCAGACGGATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAggg                                                                                                       >  2:1082834/1‑149 (MQ=255)
                                                tttGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTTCGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCATTTTTTGTTGAAAGTGAGACCTTGCCTGAAGGCGAGGTc                                                                                                      >  1:277181/1‑150 (MQ=255)
                                                     tACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGc                                                                                                 >  1:1410845/1‑150 (MQ=255)
                                                     tACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTAATTTCGATGTGTGAAATAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCTGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGc                                                                                                 <  2:210824/150‑1 (MQ=255)
                                                        cTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGAc                                                                                                                         >  2:471911/1‑123 (MQ=255)
                                                        cTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGAc                                                                                                                         <  1:471911/123‑1 (MQ=255)
                                                         tGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATaa                                                                                             >  2:802336/1‑150 (MQ=255)
                                                         tGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATaa                                                                                             <  1:46863/150‑1 (MQ=255)
                                                         tGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCATTTTTTGTTGTAAGTGAGACCTTGCAGGAAGGCGATGTCTATGCATaa                                                                                             >  1:602596/1‑150 (MQ=255)
                                                                gggAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAagcag                                                                                      >  2:834198/1‑150 (MQ=255)
                                                                    gTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGc                                                                                  <  2:1410845/150‑1 (MQ=255)
                                                                    gTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGc                                                                                  >  1:612738/1‑150 (MQ=255)
                                                                     tAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCt                                                                                 >  1:221947/1‑150 (MQ=255)
                                                                      aaCTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTGCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTg                                                                                >  1:1256875/1‑150 (MQ=255)
                                                                       aCTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGa                                                                               >  2:1313985/1‑150 (MQ=255)
                                                                               gCCGGGCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTcc                                                                       <  2:1545496/150‑1 (MQ=255)
                                                                                  ggTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGAc                                                                    >  1:46757/1‑150 (MQ=255)
                                                                                  ggTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGAc                                                                    <  1:699231/150‑1 (MQ=255)
                                                                                      gATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATATGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTg                                                                <  1:656692/150‑1 (MQ=255)
                                                                                      gATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTg                                                                <  1:449734/150‑1 (MQ=255)
                                                                                       aTCGTCATTACGATGTGTGAAAAAACACATCCGGTCACAGGGCTACCCGAAAGGAATACGAAGACGTATTCCTTTTTTGTTGTAAGTGACACCTAGCCAGAACTCGAGGAAAATCCATAAAAAGCATCGGCTGACGTCTTCAGACGATgg                                                               >  1:313732/1‑150 (MQ=255)
                                                                                         cGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGcc                                                             >  1:1229835/1‑150 (MQ=255)
                                                                                           gccttACTATGTGTTAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGACTACGCAGACGTATTCCTTTTTTGTTGTCAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGt                                                           <  1:1082834/147‑1 (MQ=255)
                                                                                            cATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGtt                                                          <  2:1563029/150‑1 (MQ=255)
                                                                                            cATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGtt                                                          >  2:458296/1‑150 (MQ=255)
                                                                                             aTTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGttt                                                         >  2:1119801/1‑150 (MQ=255)
                                                                                                   aTGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTAt                                                   <  2:602529/150‑1 (MQ=255)
                                                                                                     gtgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACGTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTAtgt                                                 >  2:224512/1‑150 (MQ=255)
                                                                                                     gtgtgAAAAACACATCCGGTCACCGGGCAACCCGAAAGCAATCCGCAGACGTATTCCTTTTTTGTTGTAAGTGACACCTTCCCGGAAGGCGAGGTCTATGCATAAAACCCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTAtgtg                                                <  2:1665006/150‑1 (MQ=255)
                                                                                                      tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTAtgtg                                                >  2:1464151/1‑150 (MQ=255)
                                                                                                      tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATACCTTTTTTGTTGTAAGTGAGACCTTGCCTGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACCTTGGCAGTTTTTTTAtgag                                                >  1:155596/1‑148 (MQ=255)
                                                                                                         gAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTaa                                             <  1:1337830/150‑1 (MQ=255)
                                                                                                          aaaaaaCACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGGTGGCCGTTTTTTTATGTGTAAg                                            >  1:325778/1‑150 (MQ=255)
                                                                                                           aaaaaCACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAgg                                           <  2:1299456/150‑1 (MQ=255)
                                                                                                           aaaaaCACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAgg                                           <  1:1518573/150‑1 (MQ=255)
                                                                                                           aaaaaCACATACCGTCCCCGGGCAACCCGAAAGGAAAACGCAGACCTAATCCTTTTTTTTTGTAAGTGAGACCATGACGGCAGGCGACGTCTATGCATAAAAAGCAGCGGCTGACCTCTTCCGACCTTTGCCGTATTTTTTTGTGTAAgg                                           >  2:466219/1‑150 (MQ=255)
                                                                                                                aacaTCCGGTCACCTGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCATCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACtt                                      <  2:691765/149‑1 (MQ=255)
                                                                                                                  caTCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCt                                    >  1:1344107/1‑150 (MQ=255)
                                                                                                                  caTCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCt                                    <  1:946106/150‑1 (MQ=255)
                                                                                                                   aTCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTa                                   <  1:1430629/150‑1 (MQ=255)
                                                                                                                    tCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTAt                                  <  2:1141672/150‑1 (MQ=255)
                                                                                                                    tCCGGTCACCGGGCAACCCAAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTAt                                  >  2:116787/1‑150 (MQ=255)
                                                                                                                     ccGGTCACCGGGCAACCCGATATGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATg                                 <  2:602596/150‑1 (MQ=255)
                                                                                                                      cGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGa                                >  1:585622/1‑150 (MQ=255)
                                                                                                                            ccGTGCAACCCGAAAGGAATACGCAGAAGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTg                          <  1:863998/150‑1 (MQ=255)
                                                                                                                               ggCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCg                       <  1:115186/150‑1 (MQ=255)
                                                                                                                               ggCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCg                       <  2:894870/150‑1 (MQ=255)
                                                                                                                                gCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCgg                      <  2:612738/150‑1 (MQ=255)
                                                                                                                                  aacgctaaaGGAATACGCAGACTTATTCCTTTTTTTTTTTAATTGAGATCTTGCCGTAAGGCGAGGTCTATTCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTTAGGAACTTCTATTAATACTGTCGGCa                    <  1:1275683/144‑1 (MQ=255)
                                                                                                                                   acaaaaaaGGAATACGTCTGCGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCAc                   >  1:89521/6‑150 (MQ=255)
                                                                                                                                    caaaaaaGGAATACGTCTGCGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTGTTTATGTGATAGGAACTTCTATGAAGACTGTCGGCACg                  >  1:1536606/5‑150 (MQ=255)
                                                                                                                                     ccGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGc                 <  1:1385877/150‑1 (MQ=255)
                                                                                                                                         aaGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGtt             <  2:670416/150‑1 (MQ=255)
                                                                                                                                             aaTACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTa         <  1:1642675/150‑1 (MQ=255)
                                                                                                                                             aaTACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTa         <  1:1529686/150‑1 (MQ=255)
                                                                                                                                              aTACGCAGACGTATTCCTTTTTTGTTGTAAGTGCGACCTTGCCGGTAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTAt        >  1:1209239/1‑150 (MQ=255)
                                                                                                                                               tACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATg       <  1:732760/150‑1 (MQ=255)
                                                                                                                                                 cGCAGACGTATTCCTTTTTTGTTGTAAGTGAGCCCTTGCCGGACGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATggg     <  1:1511737/150‑1 (MQ=255)
                                                                                                                                                  gCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATgggg    >  1:179853/1‑150 (MQ=255)
                                                                                                                                                  gCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATgggg    <  2:172565/150‑1 (MQ=255)
                                                                                                                                                   cAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGc   >  2:1116648/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GTAAAAGGTATGTAACAGACCATTGACTGGCTGAATGGTCTGTCATACTTTGTTACCTGCAAAGGGGAGTAACTTCATTGCCGGTCGATCGTCATTACGATGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCCGTTTTTTTATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCG  >  NC_000913/3238316‑3238613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: