Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,239,610 C→G 6.6% intergenic (+65/‑334) alx → / → sstT putative membrane‑bound redox modulator/sodium:serine/threonine symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,239,6100CG 6.6% 436.7 / 13.4 131intergenic (+65/‑334)alx/sstTputative membrane‑bound redox modulator/sodium:serine/threonine symporter
Reads supporting (aligned to +/- strand):  ref base C (60/62);  new base G (4/5);  total (64/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-01

Only 100 of 134 total aligned reads displayed.

GGCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATT  >  NC_000913/3239462‑3239758
                                                                                                                                                    |                                                                                                                                                    
ggCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTcc                                                                                                                                                     <  1:798293/150‑1 (MQ=255)
ggCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTcc                                                                                                                                                     <  2:1720367/150‑1 (MQ=255)
       tgtTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGgaga                                                                                                                                              >  1:1353178/1‑150 (MQ=255)
         tttGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAgg                                                                                                                                            <  2:499948/150‑1 (MQ=255)
          ttGGCATTCTGGTGATGACGTTTATTATCAACGCCTGTGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAggg                                                                                                                                           <  2:260511/150‑1 (MQ=255)
          ttGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTGGAAGGGAGAggg                                                                                                                                           <  1:698368/150‑1 (MQ=255)
          ttGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTGGAAGGGAGAggg                                                                                                                                           <  2:1185983/150‑1 (MQ=255)
          ttGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTGGAAGGGAGAggg                                                                                                                                           <  2:1263045/150‑1 (MQ=255)
             gCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCg                                                                                                                                        >  2:1654449/1‑150 (MQ=255)
              cATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCgg                                                                                                                                       <  1:1010154/150‑1 (MQ=255)
              cATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTAACGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCgg                                                                                                                                       <  2:1046304/150‑1 (MQ=255)
                  cTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTCCCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTg                                                                                                                                   >  2:875279/1‑150 (MQ=255)
                   tGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGa                                                                                                                                  <  1:1685802/150‑1 (MQ=255)
                    ggTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAg                                                                                                                                 <  1:1192942/150‑1 (MQ=255)
                     gTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAgg                                                                                                                                >  1:1483164/1‑150 (MQ=255)
                      ggatgaCGTTTATTATCCACGCCTGGGGGACTTCCCGGCATCATAAGCAGCGGGGGGGATAATTTTTAAGCTGCCTAAGCCGTGTACCCTGTCATAAACATGAGCACCGTGTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAggg                                                                                                                               <  2:1668186/149‑1 (MQ=255)
                       gatgaCGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCTTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGt                                                                                                                              >  2:496792/1‑150 (MQ=255)
                        atgaCGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTa                                                                                                                             >  1:447757/1‑150 (MQ=255)
                             gTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAtttt                                                                                                                        >  2:1180906/1‑150 (MQ=255)
                             gTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAtttt                                                                                                                        >  2:18105/1‑150 (MQ=255)
                             gTTTATTATAAACGCCTTGGTGAATGATCGGCATGTTAAGCAGCGGGGTGGATAATTTTTAATCGTGCTAAGCCGTGTTCCCTGGCATTAACATGAGCACCGTTTTCTCCCTATCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAtttt                                                                                                                        <  2:104223/150‑1 (MQ=255)
                              tttATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCCGGGAGAGGGTCGGGGTGAGGGTAAttttt                                                                                                                       >  1:1086888/1‑150 (MQ=255)
                                tattatCAACGCCTGGGTTAATTATCGGCATGATAAGCAGCGGGGTGTATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCTTTTTCTCCCTCTCCCTTCCAGGGTGAGGGTCGGGGTGAGGGTAATTTTTCg                                                                                                                     <  1:1173249/150‑1 (MQ=255)
                                tattatCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCg                                                                                                                     >  1:1008051/1‑150 (MQ=255)
                                   tatCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCAc                                                                                                                  >  2:1609174/1‑150 (MQ=255)
                                   tatCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCAc                                                                                                                  >  1:1234478/1‑150 (MQ=255)
                                           ccTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTATTTTTTCGCACCGATGCTg                                                                                                          >  2:832652/1‑150 (MQ=255)
                                            cTGGGTCAATTATCTTCATGATAAGCAGCTGTGTGTATAATTTTTAATCTGCCTAAGCCGTTTACCCTGTCATTAACATGATCACCGTTTTCTCCCTCTACCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTgg                                                                                                         <  2:1135447/150‑1 (MQ=255)
                                             tGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGc                                                                                                        <  2:732752/150‑1 (MQ=255)
                                               ggTGAATTATCGGCATCATACGCAGCGGGGTGGTTAATTTTAAATCTGCCTAAGCGGTGAACCCGGTCATTAACATGAGCTCCGTTTTGTCCATCTCCCTTCCAGGGAGAGGGTCGGGGGGAGGGTAATTTTTCGCACCGATGCTGGCCt                                                                                                      <  2:711326/150‑1 (MQ=255)
                                                 tGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGt                                                                                                    >  2:506737/1‑150 (MQ=255)
                                                   aaTTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTACCATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTc                                                                                                  <  1:96538/150‑1 (MQ=255)
                                                   aaTTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTc                                                                                                  >  2:387497/1‑150 (MQ=255)
                                                    aTTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCAGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTTAGGGTAATTTTTCGCACCGATGCTGGCCTGTTcc                                                                                                 <  1:255783/150‑1 (MQ=255)
                                                      tATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTcccc                                                                                               >  2:1130728/1‑150 (MQ=255)
                                                          ggCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCAc                                                                                           >  2:425930/1‑150 (MQ=255)
                                                           gCATGATTAGCACCGGGTGGTATAATTTTTAATCTGCCAATGCCGGGTACCCTGTCATTAACATGAGCACCGTTTTCGCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTTATTTTTCGCACCGATGCTGGCCTGTTCCCCTCAcc                                                                                          <  1:1058270/150‑1 (MQ=255)
                                                           gCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCAcc                                                                                          <  2:447757/150‑1 (MQ=255)
                                                           gCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCAcc                                                                                          >  1:589548/1‑150 (MQ=255)
                                                           gCATGAAAAGCAGCGGGGTGGATAATATTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATATTTCGCACCGATGCTGGCCTGTTCCCCTCAcc                                                                                          <  1:1063839/150‑1 (MQ=255)
                                                            cATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGCTGCTGGCCTGTTCCCCTCAccc                                                                                         >  1:92533/1‑150 (MQ=255)
                                                             aTGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCt                                                                                        <  1:1032997/150‑1 (MQ=255)
                                                                 tAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAAcc                                                                                    <  1:7104/150‑1 (MQ=255)
                                                                             tGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAc                                                                        >  2:325710/1‑150 (MQ=255)
                                                                                aTAATTTTTAATCTGACTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATCCTGGCCTGTTCCCCTCACCCTACACCTCTACCCAAACggg                                                                     >  1:157527/1‑150 (MQ=38)
                                                                                 tAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACgggg                                                                    >  1:268711/1‑150 (MQ=255)
                                                                                 tAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCATCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACgggg                                                                    <  1:404677/150‑1 (MQ=255)
                                                                                   aTTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCGCCCTAACCCTCTCCCCAAACGGGGCg                                                                  >  1:933301/1‑150 (MQ=255)
                                                                                   aTTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTACCCAAACGGGGCg                                                                  >  1:1636535/1‑150 (MQ=255)
                                                                                    tttttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGa                                                                 >  2:1346803/1‑150 (MQ=255)
                                                                                    tttttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGa                                                                 <  2:454372/150‑1 (MQ=255)
                                                                                     ttttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGGGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAg                                                                <  1:1389249/150‑1 (MQ=255)
                                                                                     ttttAAGCCGCCTAACCCGTGCACCCTGTCATTAACATGAGCACCGTTTTCACCCTCTCCCTTCCAGGGAGTGGGTCGGGGGGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAg                                                                <  2:69356/150‑1 (MQ=25)
                                                                                      tttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAgg                                                               <  2:1339056/150‑1 (MQ=255)
                                                                                      tttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAgg                                                               >  1:305726/1‑150 (MQ=255)
                                                                                       ttAATCTGCCTAAGCCGTGTACCCTGCCATTACCATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAggg                                                              <  1:767847/150‑1 (MQ=255)
                                                                                        tAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCCGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAgggg                                                             <  1:6229/150‑1 (MQ=255)
                                                                                          aTCTGCCTAAGCCGTGTCCCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGAc                                                           <  1:1615567/150‑1 (MQ=255)
                                                                                          aTCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGAc                                                           >  1:363361/1‑150 (MQ=255)
                                                                                          aTCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGAc                                                           <  2:1139724/150‑1 (MQ=255)
                                                                                          aTCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGAc                                                           >  2:322568/1‑150 (MQ=255)
                                                                                          aTCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGAc                                                           >  1:1455171/1‑150 (MQ=255)
                                                                                          aTCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGAc                                                           <  2:1244463/150‑1 (MQ=255)
                                                                                             tGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCTCCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTgc                                                        >  1:1143069/1‑149 (MQ=255)
                                                                                              gCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTACCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGCCTGAc                                                       >  1:1233872/1‑150 (MQ=255)
                                                                                                cTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCg                                                     <  2:1483164/150‑1 (MQ=255)
                                                                                                 tAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGa                                                    >  2:1107503/1‑150 (MQ=255)
                                                                                                       gtgtACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGTGACTGACCGAGTCCtt                                              <  2:591587/150‑1 (MQ=255)
                                                                                                        tgtACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCttt                                             >  1:1638074/1‑150 (MQ=255)
                                                                                                         gtACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCtttt                                            >  2:620528/1‑150 (MQ=255)
                                                                                                            cccTGTCATTAACATGCGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCCCCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCCTTTTTg                                         <  1:669241/150‑1 (MQ=255)
                                                                                                             ccTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGa                                        <  1:1609174/150‑1 (MQ=255)
                                                                                                             ccTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGa                                        <  2:1234478/150‑1 (MQ=255)
                                                                                                             ccTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGa                                        >  1:673792/1‑150 (MQ=255)
                                                                                                                     tAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGtca                                <  2:1455171/150‑1 (MQ=255)
                                                                                                                     tAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGtca                                <  2:268711/150‑1 (MQ=255)
                                                                                                                       aCATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGtcatc                              >  1:1167274/1‑150 (MQ=255)
                                                                                                                         aTGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGTGTAATTTTTCGCACCGATGCTGGCCTGTTGCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAg                            <  1:84241/150‑1 (MQ=255)
                                                                                                                         aTGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAg                            >  2:1333020/1‑150 (MQ=255)
                                                                                                                         aTGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAg                            <  2:589548/150‑1 (MQ=255)
                                                                                                                          tGAGCACCGTTTTCTCCCTCTCCCTTCCCGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGt                           <  1:482309/150‑1 (MQ=255)
                                                                                                                          tGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGt                           >  1:770395/1‑150 (MQ=255)
                                                                                                                             gCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTg                        >  2:1174853/1‑150 (MQ=255)
                                                                                                                              cACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTgg                       <  1:387497/150‑1 (MQ=255)
                                                                                                                              cACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTgg                       <  2:933301/150‑1 (MQ=255)
                                                                                                                              cACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTgg                       <  1:1130728/150‑1 (MQ=255)
                                                                                                                              cACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTgg                       >  2:589364/1‑150 (MQ=255)
                                                                                                                              cACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTgg                       >  2:606306/1‑150 (MQ=255)
                                                                                                                                       ctccctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCAc              <  1:1124731/150‑1 (MQ=255)
                                                                                                                                       ctccctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCAc              <  1:616224/150‑1 (MQ=255)
                                                                                                                                         ccctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCCCCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTGTTTTGATGTTGTCATCAGTCTGGAAGCCGCACga            >  1:1372995/1‑149 (MQ=255)
                                                                                                                                         ccctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTTATGTTGTCATCAGTCTGGAAGCCGCACGt            >  2:185452/1‑150 (MQ=255)
                                                                                                                                          cctctccctGGAAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGtt           >  2:243004/1‑150 (MQ=255)
                                                                                                                                          cctctccatTGAAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCCCCGATGCTGGCCTGTTCCCCTCCCCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATTTTTTAAGAAGTCTGGAAGACGcccttt           >  1:297018/1‑145 (MQ=255)
                                                                                                                                             ctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGc        >  1:508909/1‑150 (MQ=255)
                                                                                                                                               ccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCtt      <  1:1333020/150‑1 (MQ=255)
                                                                                                                                                cctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCttt     >  1:1219752/1‑150 (MQ=255)
                                                                                                                                                 ctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTa    <  2:1638074/150‑1 (MQ=255)
                                                                                                                                                 ctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACAGATGCTGGCGTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCTTCAGTCTGGAAGCCGCACGTTGGCtgtt    >  1:131533/1‑147 (MQ=255)
                                                                                                                                                   tCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAtt  <  1:506737/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTATT  >  NC_000913/3239462‑3239758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: