Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,246,562 C→A 6.0% intergenic (+40/‑90) exuT → / → exuR hexuronate transporter/hexuronate regulon transcriptional repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,246,5620CA 6.0% 342.4 / 7.6 108intergenic (+40/‑90)exuT/exuRhexuronate transporter/hexuronate regulon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (38/63);  new base A (4/3);  total (42/66)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

GGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGA  >  NC_000913/3246414‑3246707
                                                                                                                                                    |                                                                                                                                                 
ggCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCATtct                                                                                                                                                  >  1:828797/1‑150 (MQ=255)
 gCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTAAAAGCAGCCTtctc                                                                                                                                                 >  1:876422/1‑150 (MQ=255)
   aGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGAACCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAg                                                                                                                                               <  2:1287209/150‑1 (MQ=255)
    gtgtTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAgg                                                                                                                                              <  1:548636/150‑1 (MQ=255)
     tgtTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAggc                                                                                                                                             >  2:442681/1‑150 (MQ=255)
     tggtCGACCTGGTAGGTGCGCTTGTTATCTGGTCCGTGGTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGTTCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAggc                                                                                                                                             <  1:181569/147‑1 (MQ=255)
        tCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAggcggc                                                                                                                                          >  1:541601/1‑150 (MQ=255)
        tCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAggcggc                                                                                                                                          >  1:271162/1‑150 (MQ=255)
         cGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAggc                                                                                                                                             >  1:1571410/1‑146 (MQ=255)
         cGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAggc                                                                                                                                             <  2:1571410/146‑1 (MQ=255)
          gACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCtt                                                                                                                                        >  1:747013/1‑150 (MQ=255)
            ccTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCtttt                                                                                                                                      <  1:453320/150‑1 (MQ=255)
             cTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCttttt                                                                                                                                     >  2:1209123/1‑150 (MQ=255)
             cTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTGAGAAGGCGGCtttga                                                                                                                                     <  2:1363676/150‑3 (MQ=255)
              tGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTtc                                                                                                                                    >  1:765519/1‑150 (MQ=255)
              tGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTtc                                                                                                                                    <  1:187418/150‑1 (MQ=255)
              tGTTAGGTGCGCTGGGTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTtc                                                                                                                                    <  1:1473824/150‑1 (MQ=255)
                ttAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTGAGAAGGCGGCtttgaagg                                                                                                                                  <  1:1358097/150‑6 (MQ=255)
                 tAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTGAGAAGGCGGCtttgaaggg                                                                                                                                 <  1:1596364/150‑7 (MQ=255)
                 tAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCCGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTtcatc                                                                                                                                 <  1:1296802/150‑1 (MQ=255)
                     tGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTg                                                                                                                             <  2:1180677/150‑1 (MQ=255)
                     ggcgcTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCACCTTCTCAGGCGGCTTTTTCATCACTg                                                                                                                             <  1:228121/149‑1 (MQ=255)
                      gcgcTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGc                                                                                                                            <  1:555727/150‑1 (MQ=255)
                      gcgcTGCGTATCTGGACCGTGTGGCAGTACAAACCGGGGATTGAGGTGGCGCAGGAAACACATCACGATCCCGCACCGCAACATTTACGATTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATGACTGc                                                                                                                            <  2:817367/150‑1 (MQ=255)
                       cgcTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCg                                                                                                                           <  1:342055/150‑1 (MQ=255)
                        gcTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGa                                                                                                                          >  1:1104917/1‑150 (MQ=255)
                           ggTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTATTTTCCCTTCAAAGACGCCTTATCAGGCGACTTTTTCATCACTGCGacaa                                                                                                                       >  2:1393028/1‑147 (MQ=255)
                           ggTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAgaa                                                                                                                       >  1:1690743/1‑148 (MQ=255)
                              tATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTagag                                                                                                                    >  1:301891/1‑150 (MQ=255)
                                tCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTTTAGCt                                                                                                                  >  2:1226288/1‑150 (MQ=255)
                                tCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCt                                                                                                                  <  2:884671/150‑1 (MQ=255)
                                  tGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTaa                                                                                                                >  2:245527/1‑150 (MQ=255)
                                   ggACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTaaa                                                                                                               >  1:519942/1‑150 (MQ=255)
                                   ggACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTaaa                                                                                                               >  2:1192766/1‑150 (MQ=255)
                                       cGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCg                                                                                                           <  2:204309/150‑1 (MQ=255)
                                        gtgtTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGc                                                                                                          <  1:952346/150‑1 (MQ=255)
                                          gtTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTTTAGCTCTACTCGCag                                                                                                        <  1:613845/150‑2 (MQ=255)
                                          gtTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCaa                                                                                                        <  1:948255/150‑1 (MQ=255)
                                             gCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTTTAGCTCTACTCGCagtga                                                                                                     <  1:1226288/150‑5 (MQ=255)
                                                gAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCTGCTTTTTCATCACTGAGAGTAGAGCTAAACTCGCATAAGTgg                                                                                                  <  2:876422/150‑1 (MQ=255)
                                                gAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCAGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGTGAGTAGAGCTAAACTCGCAAAAGTgg                                                                                                  <  1:1022228/150‑1 (MQ=255)
                                                 aaCAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGt                                                                                                 >  2:62042/1‑150 (MQ=255)
                                                 aaCAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGt                                                                                                 <  1:1147055/150‑1 (MQ=255)
                                                   cAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGtat                                                                                               <  2:529419/150‑1 (MQ=255)
                                                           cGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACaaa                                                                                         <  1:941519/148‑1 (MQ=255)
                                                           cGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACaaa                                                                                         >  2:941519/1‑148 (MQ=255)
                                                           cGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAata                                                                                       >  1:326651/1‑150 (MQ=255)
                                                            gATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAatat                                                                                      <  2:1440591/150‑1 (MQ=255)
                                                            gATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAAAGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAatat                                                                                      <  1:1063723/150‑1 (MQ=255)
                                                             aTTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTATGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAATAGTGGTATAACAAAtata                                                                                     >  1:834539/1‑150 (MQ=255)
                                                             aTTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAAtata                                                                                     <  2:105597/150‑1 (MQ=255)
                                                              ttGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAg                                                                                    <  2:613881/150‑1 (MQ=255)
                                                              ttGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAg                                                                                    <  1:515531/150‑1 (MQ=255)
                                                               tGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGt                                                                                   <  2:301891/150‑1 (MQ=255)
                                                                gAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTg                                                                                                   >  1:1220447/1‑133 (MQ=255)
                                                                gAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACa                                                                                           <  2:1413112/141‑1 (MQ=255)
                                                                gAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACa                                                                                           >  1:1413112/1‑141 (MQ=255)
                                                                 aGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCt                                                                                 <  1:1176113/150‑1 (MQ=255)
                                                                 aGGTGGCGCAGGAAACAAATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAATGTGGTATAACAAATATAGTCt                                                                                 >  2:116453/1‑150 (MQ=255)
                                                                   gTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCCGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGc                                                                               >  1:146178/1‑150 (MQ=255)
                                                                              atcacaTAACGATCCCGAACCGCTACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGcc                                                                    <  2:882053/148‑1 (MQ=255)
                                                                              aaCACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGcc                                                                    >  1:743300/1‑150 (MQ=255)
                                                                               acacATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCt                                                                   <  2:271162/150‑1 (MQ=255)
                                                                                 acaTAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTgg                                                                 <  2:1307/150‑1 (MQ=255)
                                                                                  caTAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGa                                                                <  1:1314102/150‑1 (MQ=255)
                                                                                   aTAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAg                                                               <  1:427031/150‑1 (MQ=255)
                                                                                      aCGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTTCCGTATCATGCCTGGAgcgc                                                            <  1:56755/150‑1 (MQ=255)
                                                                                      aCGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAgcgc                                                            <  2:519942/150‑1 (MQ=255)
                                                                                       cGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCa                                                           >  1:541210/1‑150 (MQ=255)
                                                                                         aTCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCata                                                         <  1:1260876/150‑1 (MQ=255)
                                                                                           cccGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATg                                                       <  1:1476740/150‑1 (MQ=255)
                                                                                            ccgcaccgcaACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATgg                                                      <  1:442681/150‑1 (MQ=255)
                                                                                            ccgcaccgcaACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATgg                                                      <  2:765519/150‑1 (MQ=255)
                                                                                             cgcaccgcaACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGa                                                     >  1:166663/1‑150 (MQ=255)
                                                                                               caccgcaACATTAAAGTTTCGTTTAAGTATTAGTTTCCCTTCAAAGCCGCCTTCTCAGTCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGaaa                                                   <  2:483286/150‑1 (MQ=255)
                                                                                                 ccgcaACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATc                                                 >  2:1034688/1‑150 (MQ=255)
                                                                                                   gcaACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCAc                                               >  1:1704395/1‑150 (MQ=255)
                                                                                                   gcaACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCAc                                               >  2:78842/1‑150 (MQ=255)
                                                                                                     aaCATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTg                                             >  2:1357559/1‑150 (MQ=255)
                                                                                                        aTTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAAc                                          <  1:1192766/150‑1 (MQ=255)
                                                                                                        aTTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAAc                                          >  2:1141663/1‑150 (MQ=255)
                                                                                                            aCGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACg                                      >  1:213595/1‑150 (MQ=255)
                                                                                                                 tCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGtt                                 >  1:415906/1‑150 (MQ=255)
                                                                                                                         gTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCGTTTTCATCACTGCGAGTAGAGCTAAAGTCGCATCAGTGGTATAACAAATATAGTCTGCCGTATCCTGCCTGGAGCGCATATGGAAATCACTGCACCACGCCCTTTGTATcaa                         >  2:754443/1‑150 (MQ=255)
                                                                                                                            ttAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATcaacaa                      >  1:354976/1‑150 (MQ=255)
                                                                                                                             tAGTTTCCCTTCACAGCCGCCTACTCAGGCGGGTTTTTCATGACTGCTAGTAGATCTAAACTCGCAAAAGTGGTATAACAAATCAAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGACCCACGCCGGTTGTATCaacaac                     <  2:1545143/150‑1 (MQ=255)
                                                                                                                                tttCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTg                  <  1:542610/150‑1 (MQ=255)
                                                                                                                                tttCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGAAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTg                  <  2:1690743/150‑1 (MQ=255)
                                                                                                                                  tCCCTTCAAAGCCGCCTTCTCATGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCAGTATCATGCCTGGAGCTCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGcc                <  1:1393028/150‑1 (MQ=255)
                                                                                                                                  tCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGcc                >  1:1153117/1‑150 (MQ=255)
                                                                                                                                    ccTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGc              <  2:326651/150‑1 (MQ=255)
                                                                                                                                     tgaagaaGCCGCCTGAGAAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTAGCctct             >  2:1155157/6‑150 (MQ=255)
                                                                                                                                     cTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCt             <  2:166663/150‑1 (MQ=255)
                                                                                                                                      gaaaaaGCCGCCTGAGAAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTg            >  2:410995/4‑150 (MQ=255)
                                                                                                                                       tCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGa           <  2:1679910/150‑1 (MQ=255)
                                                                                                                                       aaaaaGCCGCCTGAGAAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGa           >  2:438288/3‑150 (MQ=255)
                                                                                                                                        cAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGGTAAAATCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGAc          <  2:828797/150‑1 (MQ=255)
                                                                                                                                           aGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTg       <  1:1505475/150‑1 (MQ=255)
                                                                                                                                               gccTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAag   <  2:1436127/150‑1 (MQ=255)
                                                                                                                                                ccTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAaga  <  2:1187014/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GGCAGTGTTCGACCTGTTAGGTGCGCTGGTTATCTGGACCGTGTTGCAGAACAAACCGGCGATTGAGGTGGCGCAGGAAACACATAACGATCCCGCACCGCAACATTAACGTTTCGTGTAAGTTTTAGTTTCCCTTCAAAGCCGCCTTCTCAGGCGGCTTTTTCATCACTGCGAGTAGAGCTAAACTCGCAAAAGTGGTATAACAAATATAGTCTGCCGTATCATGCCTGGAGCGCATATGGAAATCACTGAACCACGCCGTTTGTATCAACAACTTGCCGCTGACCTGAAAGA  >  NC_000913/3246414‑3246707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: