Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,256,441 T→G 10.1% T71P (ACG→CCG)  yhaM ← putative L‑serine dehydratase alpha chain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,256,4410TG10.1% 323.2 / 22.9 133T71P (ACG→CCG) yhaMputative L‑serine dehydratase alpha chain
Reads supporting (aligned to +/- strand):  ref base T (57/62);  new base G (8/6);  total (65/68)
Fisher's exact test for biased strand distribution p-value = 5.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.91e-01

Only 100 of 133 total aligned reads displayed.

ATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTT  >  NC_000913/3256293‑3256588
                                                                                                                                                    |                                                                                                                                                   
aTCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTg                                                                                                                                                    >  1:1407702/1‑150 (MQ=255)
    tAACGGAGCCTTTCCCCGCCGCCAGCAGTGCGTTGGCCTCGGCAATTGCCTGCGCCGTTGCGTCTTTCAGCACGTCCAGCCCCGCGTTGGCATTACCACCTCACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCgg                                                                                                                                                <  1:327777/150‑1 (MQ=255)
        ggAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAAc                                                                                                                                            >  2:696054/1‑150 (MQ=255)
        ggAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCAGGTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGGGCCGCGAAc                                                                                                                                            >  1:152247/1‑150 (MQ=255)
         gagaCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCggg                                                                                                                                               <  2:1062534/146‑1 (MQ=255)
          cgaCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCGGTTGCGTCTTTCAGCACTTCCAGCCCGGCGGTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGGCACGGGAATgg                                                                                                                                          <  2:206871/149‑1 (MQ=255)
           gaCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGt                                                                                                                                         >  1:94444/1‑150 (MQ=255)
           gaCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGACATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGt                                                                                                                                         <  1:179399/150‑1 (MQ=255)
             cTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTTACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGa                                                                                                                                       <  2:433273/150‑1 (MQ=255)
              tttCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGAc                                                                                                                                      >  2:1404452/1‑150 (MQ=255)
               ttCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCGTTCCCGGCACGGGAATGGTgggg                                                                                                                                     <  1:198574/150‑4 (MQ=255)
                   ccgccgCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGCCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCAACAATCCCGTGCCGGGAACGGTGACGCCCa                                                                                                                                 >  1:272623/1‑150 (MQ=255)
                        gccAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGTATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCGTTCCCGGCACGGGAATGGTg                                                                                                                                        <  2:1498005/138‑1 (MQ=255)
                        gccAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCg                                                                                                                            >  2:375904/1‑150 (MQ=255)
                            gcagTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGttct                                                                                                                        >  2:977870/1‑150 (MQ=255)
                             cagTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCTCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGttctt                                                                                                                       <  1:375904/150‑1 (MQ=255)
                               gTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCCGACCGTTCTtca                                                                                                                     >  1:910689/1‑150 (MQ=255)
                               gTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTtca                                                                                                                     >  2:597019/1‑150 (MQ=255)
                                   tttGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCCGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGGGCCGGGACCGGTGACGCCCAGACCGTTCTGCCTCAg                                                                                                                 >  1:1518824/1‑150 (MQ=255)
                                        cATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGCCCGTGCTTCATCAGATTTg                                                                                                            >  1:509877/1‑150 (MQ=255)
                                        cATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTg                                                                                                            <  1:274415/150‑1 (MQ=255)
                                         aTCGGCAATTGCCGGCGGTGTTGCGTCTTTCAGCACTTCCAGCCCGGGGTTGGAATTTCCACCTAACGCCCCCTGCGCCGCCGCACTCGGCAGCCCCACCATTCCCGTGCGGGGAATGGTGACGCCCAGACCGTTCTTCATCAGATTTgg                                                                                                           <  2:1637826/150‑1 (MQ=255)
                                          tCGGCAATGGCCTGCGCTGTGGCGGCGTGCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCGAGCGCCGCCGCAATCTGCAGCCCCACCATTCCCGTGCCGTGAACGGTGACGCCCAGACCGTTCTGCATCAGATTTGGc                                                                                                          <  2:1054266/150‑1 (MQ=255)
                                           cGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCg                                                                                                         <  2:372209/150‑1 (MQ=255)
                                             gCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGaa                                                                                                       >  2:271445/1‑150 (MQ=255)
                                              cAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTg                                                                                                            >  2:1683346/1‑144 (MQ=255)
                                              cAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTg                                                                                                            <  1:1683346/144‑1 (MQ=255)
                                                aTTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGACACAAGCTCCGCCGCAATCGGCAGCCCCACCATTCCCGTGACGGGAACGGTGACGCCCAGAACGTGATTCAGAAGATTTGGCGAATcc                                                                                                    >  1:341444/1‑150 (MQ=255)
                                                     cTGCGCTGTGGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGc                                                                                               <  1:1491365/150‑1 (MQ=255)
                                                        cgcTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTc                                                                                            >  1:1706488/1‑150 (MQ=255)
                                                          cTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTa                                                                                          >  2:1136781/1‑150 (MQ=255)
                                                            gTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTaca                                                                                        <  1:271445/150‑1 (MQ=255)
                                                              tGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACg                                                                                      >  1:424352/1‑150 (MQ=255)
                                                              tGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACg                                                                                      <  1:1360837/150‑1 (MQ=255)
                                                              tGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTGCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACg                                                                                      <  2:487978/150‑1 (MQ=255)
                                                              ggCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACg                                                                                      <  2:841669/149‑1 (MQ=255)
                                                                 gTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTc                                                                                   >  2:892700/1‑150 (MQ=255)
                                                                 gTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTc                                                                                   <  1:1718140/150‑1 (MQ=255)
                                                                   cTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGAACGTTCTTCAGCAGATTTGGCGAAACCCAGGCTTCGACACGTTCac                                                                                 >  2:1684377/1‑149 (MQ=255)
                                                                    tttCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAAc                                                                                <  1:696054/150‑1 (MQ=255)
                                                                      tCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCGAGCGCCGCCGCAATCGGCAGCCCCAACATTCCCGTGCCGGGAACGTTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCg                                                                              <  2:390135/150‑1 (MQ=255)
                                                                        aGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGa                                                                            >  1:101055/1‑150 (MQ=255)
                                                                              tCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGCCGCCCAGACCGTTCTTCCGCAGAGTTGGCGACACCCCGGCTGCTACACGTTGAACCGGTCCTTcc                                                                      >  1:1067389/1‑150 (MQ=255)
                                                                              tCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTcc                                                                      >  1:661106/1‑150 (MQ=255)
                                                                               ccAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCa                                                                     <  1:1136781/150‑1 (MQ=255)
                                                                                cAGCCCGGCGTTTGCATTTCCACCTAACGCCCCCAGCGCCGCCGCATTCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACTCGTTCAACCGGACCTTCCAg                                                                    <  1:723019/150‑1 (MQ=255)
                                                                                cAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAg                                                                    >  2:807550/1‑150 (MQ=255)
                                                                                  gCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGtt                                                                  >  2:1205093/1‑150 (MQ=255)
                                                                                  gCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCGTCCAGtt                                                                  >  1:1690182/1‑150 (MQ=255)
                                                                                     cGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTg                                                               <  1:1123227/150‑1 (MQ=255)
                                                                                        cGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCg                                                            <  2:1306191/150‑1 (MQ=255)
                                                                                         gTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCgc                                                           <  1:1227104/150‑1 (MQ=255)
                                                                                          ttGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCGTCATCAGATTTGGCGAAACCCAGGCGTCTCCACGTTCACCCGGACCTTCCAGTTCTGCCgcc                                                          >  1:1485393/1‑149 (MQ=255)
                                                                                           tGGCTTTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCgcag                                                         <  2:152247/150‑1 (MQ=255)
                                                                                            ggCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCgcagc                                                        <  2:94444/150‑1 (MQ=255)
                                                                                             gCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAACCCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCgcagca                                                       >  1:1014362/1‑150 (MQ=255)
                                                                                              cATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTAGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCaa                                                      >  2:510527/1‑150 (MQ=255)
                                                                                                tttCCCCCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGGGCCGGGAGCGGTGACCCGCAGACGGGTCGTCATCAGAATTGGCGAAAACCAGACTTCTAACGGTTCAACCGGACATTCTAGTTCGGGCGCTGCAccc                                                    >  2:349507/1‑150 (MQ=255)
                                                                                                   ccACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAAccgcc                                                 >  1:1040072/1‑150 (MQ=255)
                                                                                                    cACCTAACGCCCCCATCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAAccgccg                                                <  1:1259721/150‑1 (MQ=255)
                                                                                                    cACCTAACGCCCCCAGCGCCGCCGCAATCGGCTGCCCCACCATTCCCGTGCCGGGAACGGTGACGGCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCTACCTGACCTTCCAGTTCTGCCGCAGCAAccgccg                                                <  1:1266479/150‑1 (MQ=255)
                                                                                                    cACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAAccgccg                                                <  1:1025968/150‑1 (MQ=255)
                                                                                                      ccTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAAccgccgcc                                              >  2:535346/1‑150 (MQ=255)
                                                                                                        tAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCCACCGGACCTTCCAGTTCTGCCGCAGCAACcgccgccgc                                            >  1:1144733/1‑150 (MQ=255)
                                                                                                        tAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACcgccgccgc                                            >  2:1527002/1‑150 (MQ=255)
                                                                                                        tAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGAGTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACcgccgccgc                                            >  2:1034432/1‑150 (MQ=255)
                                                                                                            gCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGGCGCCCAGACCGTTCGTCATCAGCTTTGGCGAAACCCAGGCTTCTACACGTTCCACCGGACCTTCCAGTTCTGCCGCAGCCACCGCCGCcgccagg                                        >  1:328359/1‑149 (MQ=255)
                                                                                                            gCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCcgccagc                                        >  2:333093/1‑150 (MQ=255)
                                                                                                             cccccAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCAGGGTAAGGTGACGCCCAGACCCTTCTTCATCAGATTTGGCGCAACCCAGGCTTCTACACGTTAAACCGGACCTTCCCGTTCTGCCGCAGCAACCGCCGCcgccagcg                                       >  1:290589/1‑150 (MQ=255)
                                                                                                              ccccAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCcgccagcgc                                      >  1:747507/1‑150 (MQ=255)
                                                                                                              ccccAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCcgccagcgc                                      >  1:1062868/1‑150 (MQ=255)
                                                                                                               cccAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCcgccagcgcc                                     >  2:1264026/1‑150 (MQ=255)
                                                                                                                ccAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGGCCGTTCTTCATCAGATTTGGCGAAAACCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCTCACCAACCGCCGCcgcaatcgcca                                    >  1:197377/1‑150 (MQ=255)
                                                                                                                 cAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCcgccagcgccag                                   <  1:977870/150‑1 (MQ=255)
                                                                                                                 cAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCcgccagcgccag                                   >  1:363030/1‑150 (MQ=255)
                                                                                                                  aGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGt                                  <  2:1464542/150‑1 (MQ=255)
                                                                                                                  aGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGt                                  <  2:1717177/150‑1 (MQ=255)
                                                                                                                   gcgcCGCCGCAATCGGCAGCCCCTCCATTCCCGTGCCGGGAACGGTTACGCCCAGACCGTTCTTCATCAGATTTGGCGATACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCATCAACCGCCGCCGCCAGCGCCAGTg                                 <  2:47243/150‑1 (MQ=255)
                                                                                                                    cgccgccgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTGCTTCATCAGATTTGGCGACACCCAGGCTTCTACACGATCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGa                                <  1:216103/150‑1 (MQ=255)
                                                                                                                     gccgccgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGaa                               <  2:1040072/150‑1 (MQ=255)
                                                                                                                       cgccgcAATCGGCCGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCACCCGGACCTTCCAGTTCTGCCGCAGCACCCGCCGCCGCCCGCGCCAGTGCAAt                             >  2:1514484/1‑150 (MQ=255)
                                                                                                                        gccgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCCGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCGTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCACTGAAATc                            >  1:912666/1‑150 (MQ=255)
                                                                                                                        gccgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCCGGCTTCTACCCGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGGGAAATc                            >  1:1064706/1‑150 (MQ=255)
                                                                                                                        gccgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATc                            <  1:430506/150‑1 (MQ=255)
                                                                                                                        gccgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATc                            <  1:798874/150‑1 (MQ=255)
                                                                                                                        gccgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCGTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATc                            >  2:1283861/1‑150 (MQ=255)
                                                                                                                          cgcAATCGGCAGCCCCACCCTTCCCGTGCCGGGAACTGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAACCCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCTGCAACCGCCGCCGCCAGCGCCAGTGAAATCgg                          <  1:54374/150‑1 (MQ=255)
                                                                                                                            cAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGtt                        <  1:1264026/150‑1 (MQ=255)
                                                                                                                             aaTCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTc                       <  1:1716677/150‑1 (MQ=255)
                                                                                                                                      cgtcaccgTTCCCGGCACGGGAATGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATcc              >  1:839711/4‑150 (MQ=255)
                                                                                                                                      ccccACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATcc              >  1:1707412/1‑150 (MQ=255)
                                                                                                                                       cccACCATTCCCGTGCCGGGAACGGTGACGCCCAGACGGTTCTTCATCAGATTTGGCGAAACCCTGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCTACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATccc             <  2:328359/150‑1 (MQ=255)
                                                                                                                                       cccACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCACCCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGACATCGGTTCAGTACATccc             >  1:132652/1‑150 (MQ=255)
                                                                                                                                        tcaccgTTCCCGGCACGGGAATGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCCGCAACCGCCGCCGCCAGCGCCAGTGAAATCTGTTCAGTACATccgc            >  2:149768/2‑148 (MQ=255)
                                                                                                                                         caccaTTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGATATCGGTTCAGTACATCCCAg           <  2:747507/150‑1 (MQ=255)
                                                                                                                                         caccaTTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAg           <  2:1062868/150‑1 (MQ=255)
                                                                                                                                         caccaTTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAg           <  2:963291/150‑1 (MQ=255)
                                                                                                                                          accgTTCCCGGCACGGGAATGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAgc          >  2:708185/1‑150 (MQ=255)
                                                                                                                                                cccGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCTGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGACGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGt    <  1:869150/150‑1 (MQ=255)
                                                                                                                                                  cGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGttt  >  1:527938/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ATCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTT  >  NC_000913/3256293‑3256588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: