Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,256,443 C→A 8.2% G70V (GGC→GTC) ‡ yhaM ← putative L‑serine dehydratase alpha chain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,256,4430CA 8.2% 371.1 / 15.6 133G70V (GGC→GTC) ‡yhaMputative L‑serine dehydratase alpha chain
Reads supporting (aligned to +/- strand):  ref base C (58/62);  new base A (6/6);  total (64/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

Only 100 of 135 total aligned reads displayed.

TCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTACT  >  NC_000913/3256294‑3256592
                                                                                                                                                     |                                                                                                                                                     
tCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGTCATCGGCAATTGCCTGCGCTGCTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGc                                                                                                                                                       <  1:267031/150‑1 (MQ=255)
       ggAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCAGGTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGGGCCGCGAAc                                                                                                                                                >  1:152247/1‑150 (MQ=255)
        gagaCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCggg                                                                                                                                                   <  2:1062534/146‑1 (MQ=255)
        gagaCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCggg                                                                                                                                                   >  1:1062534/1‑146 (MQ=255)
        gagaCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACg                                                                                                                                               <  1:1120237/150‑1 (MQ=255)
         agaCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACgg                                                                                                                                              <  1:1398994/150‑1 (MQ=255)
          gaCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGt                                                                                                                                             >  1:94444/1‑150 (MQ=255)
          gaCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGACATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGt                                                                                                                                             <  1:179399/150‑1 (MQ=255)
           aCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTGGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCGTTCCCGGCACGGGAATGGTg                                                                                                                                            <  1:18463/150‑1 (MQ=255)
             tttCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCCGCCCCACCATTCCCGTGCCGGGAACGGTGAc                                                                                                                                          >  2:1264312/1‑150 (MQ=255)
              ttCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTATCAGCACTTCCAGCCGGGCGTGGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGGCACGGGAATGGTgggg                                                                                                                                         <  1:407376/150‑4 (MQ=255)
                  ccgccgCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGACGCAATCGGCAGCCCCACCATTAACTTGCAGGGAACGGTGACGCCCa                                                                                                                                     >  2:502344/1‑150 (MQ=255)
                       gccAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCg                                                                                                                                >  2:375904/1‑150 (MQ=255)
                           gcagTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGttct                                                                                                                            >  2:977870/1‑150 (MQ=255)
                            cagTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCTCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGttctt                                                                                                                           <  1:375904/150‑1 (MQ=255)
                              gTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCCGACCGTTCTtca                                                                                                                         >  1:910689/1‑150 (MQ=255)
                              gTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTtca                                                                                                                         >  2:597019/1‑150 (MQ=255)
                                gCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTtcatc                                                                                                                       >  2:1442034/1‑150 (MQ=255)
                                  tttGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCCGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGGGCCGGGACCGGTGACGCCCAGACCGTTCTGCCTCAg                                                                                                                     >  1:1518824/1‑150 (MQ=255)
                                  tttGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGGCGGGAACGGTGACGCCCAGACCGTTCTTCATCAg                                                                                                                     <  1:1713735/150‑1 (MQ=255)
                                       cATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGCCCGTGCTTCATCAGATTTg                                                                                                                >  1:509877/1‑150 (MQ=255)
                                       cATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTg                                                                                                                <  1:274415/150‑1 (MQ=255)
                                        aTCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTgg                                                                                                               <  2:29900/150‑1 (MQ=255)
                                        aTCGGCAATTGCCGGCGGTGTTGCGTCTTTCAGCACTTCCAGCCCGGGGTTGGAATTTCCACCTAACGCCCCCTGCGCCGCCGCACTCGGCAGCCCCACCATTCCCGTGCGGGGAATGGTGACGCCCAGACCGTTCTTCATCAGATTTgg                                                                                                               <  2:1637826/150‑1 (MQ=255)
                                         tCGGCAATGGCCTGCGCTGTGGCGGCGTGCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCGAGCGCCGCCGCAATCTGCAGCCCCACCATTCCCGTGCCGTGAACGGTGACGCCCAGACCGTTCTGCATCAGATTTGGc                                                                                                              <  2:1054266/150‑1 (MQ=255)
                                            gCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGaa                                                                                                           <  2:1570278/150‑1 (MQ=255)
                                            gCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGaa                                                                                                           >  2:271445/1‑150 (MQ=255)
                                             cAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTg                                                                                                                <  1:1683346/144‑1 (MQ=255)
                                             cAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGaaa                                                                                                          <  2:393911/150‑1 (MQ=255)
                                               aTTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGACACAAGCTCCGCCGCAATCGGCAGCCCCACCATTCCCGTGACGGGAACGGTGACGCCCAGAACGTGATTCAGAAGATTTGGCGAATcc                                                                                                        >  1:341444/1‑150 (MQ=255)
                                                    cTGCGCTGTGGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGc                                                                                                   <  1:1491365/150‑1 (MQ=255)
                                                        gcTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCt                                                                                               <  2:1106426/150‑1 (MQ=255)
                                                         cTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTa                                                                                              >  2:798874/1‑150 (MQ=255)
                                                         cTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTa                                                                                              >  2:1136781/1‑150 (MQ=255)
                                                           gTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTaca                                                                                            <  1:271445/150‑1 (MQ=255)
                                                             tGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTGCTACACg                                                                                          >  1:289540/1‑150 (MQ=255)
                                                             tGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTGCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACg                                                                                          <  2:487978/150‑1 (MQ=255)
                                                             ggCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACg                                                                                          <  2:841669/149‑1 (MQ=255)
                                                                gTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTc                                                                                       >  2:892700/1‑150 (MQ=255)
                                                                  cTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGAACGTTCTTCAGCAGATTTGGCGAAACCCAGGCTTCGACACGTTCac                                                                                     >  2:1684377/1‑149 (MQ=255)
                                                                   tttCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAAc                                                                                    <  1:696054/150‑1 (MQ=255)
                                                                     tCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCGAGCGCCGCCGCAATCGGCAGCCCCAACATTCCCGTGCCGGGAACGTTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCg                                                                                  <  2:390135/150‑1 (MQ=255)
                                                                       aGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAACCCCAGGCTTCTACACGTTCCACCggc                                                                                >  2:1715027/1‑149 (MQ=255)
                                                                       aGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGa                                                                                >  1:101055/1‑150 (MQ=255)
                                                                             tCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTcc                                                                          >  1:661106/1‑150 (MQ=255)
                                                                             tCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTcc                                                                          <  1:1683888/150‑1 (MQ=255)
                                                                              ccAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCa                                                                         <  1:1136781/150‑1 (MQ=255)
                                                                               cAGCCCGGCGTTTGCATTTCCACCTAACGCCCCCAGCGCCGCCGCATTCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACTCGTTCAACCGGACCTTCCAg                                                                        <  1:723019/150‑1 (MQ=255)
                                                                                 gCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGtt                                                                      >  2:1205093/1‑150 (MQ=255)
                                                                                 gCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCGTCCAGtt                                                                      >  1:1690182/1‑150 (MQ=255)
                                                                                    cGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTg                                                                   <  1:1123227/150‑1 (MQ=255)
                                                                                    cGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTg                                                                   <  2:1510474/150‑1 (MQ=255)
                                                                                       cGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCg                                                                <  2:1306191/150‑1 (MQ=255)
                                                                                        gTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCgc                                                               <  1:1227104/150‑1 (MQ=255)
                                                                                         ttGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCGTCATCAGATTTGGCGAAACCCAGGCGTCTCCACGTTCACCCGGACCTTCCAGTTCTGCCgcc                                                              >  1:1485393/1‑149 (MQ=255)
                                                                                           ggCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGAGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCgcagc                                                            >  2:694334/1‑150 (MQ=255)
                                                                                            gCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAACCCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCgcagca                                                           >  1:1014362/1‑150 (MQ=255)
                                                                                              aTTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTGCACGTTCAACCGGCCCGTGCAGTTCTGCCgcagcatc                                                         >  1:339168/1‑148 (MQ=255)
                                                                                               tttCCCCCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGGGCCGGGAGCGGTGACCCGCAGACGGGTCGTCATCAGAATTGGCGAAAACCAGACTTCTAACGGTTCAACCGGACATTCTAGTTCGGGCGCTGCAccc                                                        >  2:349507/1‑150 (MQ=255)
                                                                                                   cACCTAACGCCCCCATCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAAccgccg                                                    <  1:1259721/150‑1 (MQ=255)
                                                                                                   cACCTAACGCCCCCAGCGCCGCCGCAATCGGCTGCCCCACCATTCCCGTGCCGGGAACGGTGACGGCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCTACCTGACCTTCCAGTTCTGCCGCAGCAAccgccg                                                    <  1:1266479/150‑1 (MQ=255)
                                                                                                   cACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAAccgccg                                                    <  1:1025968/150‑1 (MQ=255)
                                                                                                     ccTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAAccgccgcc                                                  >  2:535346/1‑150 (MQ=255)
                                                                                                       tAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACcgccgccgc                                                >  2:1527002/1‑150 (MQ=255)
                                                                                                       tAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGAGTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACcgccgccgc                                                >  2:1034432/1‑150 (MQ=255)
                                                                                                           gCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGGCGCCCAGACCGTTCGTCATCAGCTTTGGCGAAACCCAGGCTTCTACACGTTCCACCGGACCTTCCAGTTCTGCCGCAGCCACCGCCGCcgccagg                                            >  1:328359/1‑149 (MQ=255)
                                                                                                           gCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCcgccagc                                            >  2:333093/1‑150 (MQ=255)
                                                                                                            cccccAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCcgccagcg                                           >  2:893922/1‑150 (MQ=255)
                                                                                                            cccccAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCAGGGTAAGGTGACGCCCAGACCCTTCTTCATCAGATTTGGCGCAACCCAGGCTTCTACACGTTAAACCGGACCTTCCCGTTCTGCCGCAGCAACCGCCGCcgccagcg                                           >  1:290589/1‑150 (MQ=255)
                                                                                                             ccccAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCcgccagcgc                                          >  1:1062868/1‑150 (MQ=255)
                                                                                                             ccccAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCcgccagcgc                                          >  1:747507/1‑150 (MQ=255)
                                                                                                               ccAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGGCCGTTCTTCATCAGATTTGGCGAAAACCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCTCACCAACCGCCGCcgcaatcgcca                                        >  1:197377/1‑150 (MQ=255)
                                                                                                                cAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCcgccagcgccag                                       <  1:977870/150‑1 (MQ=255)
                                                                                                                 aGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGt                                      <  2:1464542/150‑1 (MQ=255)
                                                                                                                 aGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGt                                      <  2:1717177/150‑1 (MQ=255)
                                                                                                                  gcgcCGCCGCAATCGGCAGCCCCTCCATTCCCGTGCCGGGAACGGTTACGCCCAGACCGTTCTTCATCAGATTTGGCGATACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCATCAACCGCCGCCGCCAGCGCCAGTg                                     <  2:47243/150‑1 (MQ=255)
                                                                                                                   cgccgccgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTGCTTCATCAGATTTGGCGACACCCAGGCTTCTACACGATCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGa                                    <  1:216103/150‑1 (MQ=255)
                                                                                                                    gccgccgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGaa                                   <  2:1040072/150‑1 (MQ=255)
                                                                                                                      cgccgcAATCGGCCGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCACCCGGACCTTCCAGTTCTGCCGCAGCACCCGCCGCCGCCCGCGCCAGTGCAAt                                 >  2:1514484/1‑150 (MQ=255)
                                                                                                                       gccgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCCGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCGTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCACTGAAATc                                >  1:912666/1‑150 (MQ=255)
                                                                                                                       gccgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCCGGCTTCTACCCGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGGGAAATc                                >  1:1064706/1‑150 (MQ=255)
                                                                                                                       gccgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATc                                <  1:798874/150‑1 (MQ=255)
                                                                                                                       gccgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATc                                <  1:430506/150‑1 (MQ=255)
                                                                                                                       gccgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCGTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATc                                >  2:1283861/1‑150 (MQ=255)
                                                                                                                        ccgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCg                               <  1:1191347/150‑1 (MQ=255)
                                                                                                                         cgcAATCGGCAGCCCCACCCTTCCCGTGCCGGGAACTGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAACCCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCTGCAACCGCCGCCGCCAGCGCCAGTGAAATCgg                              <  1:54374/150‑1 (MQ=255)
                                                                                                                            aaTCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTc                           <  1:1716677/150‑1 (MQ=255)
                                                                                                                                ggCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCTTTATTTATAATTTTTGGCGAAACACAGTCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTa                       <  2:3710/150‑1 (MQ=255)
                                                                                                                                     cgtcaccgTTCCCGGCACGGGAATGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATcc                  >  1:839711/4‑150 (MQ=255)
                                                                                                                                     ccccACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATcc                  >  1:1707412/1‑150 (MQ=255)
                                                                                                                                      cccACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCACCCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGACATCGGTTCAGTACATccc                 >  1:132652/1‑150 (MQ=255)
                                                                                                                                      cccACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATccc                 <  1:1264312/150‑1 (MQ=255)
                                                                                                                                       tcaccgTTCCCGGCACGGGAATGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCCGCAACCGCCGCCGCCAGCGCCAGTGAAATCTGTTCAGTACATccgc                >  2:149768/2‑148 (MQ=255)
                                                                                                                                        caccaTTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGATATCGGTTCAGTACATCCCAg               <  2:747507/150‑1 (MQ=255)
                                                                                                                                        caccaTTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAg               <  2:963291/150‑1 (MQ=255)
                                                                                                                                         accgTTCCCGGCACGGGAATGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAgc              >  2:708185/1‑150 (MQ=255)
                                                                                                                                             ttCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCGAGTGAAATCGGTTCAGTACATCCCAGCGCCg          <  1:510527/150‑1 (MQ=255)
                                                                                                                                               cccGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCTGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGACGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGt        <  1:869150/150‑1 (MQ=255)
                                                                                                                                                   ggCCGGTAACGGGGACGCCCAGACCGTTCTGCATCAGATTTGCCGAACCCCAGGCTTCTCCCCGTTCAACCGGCCCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTa    <  2:1518824/149‑1 (MQ=255)
                                                                                                                                                     ccGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTACt  <  1:639574/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCTTAACGGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTACT  >  NC_000913/3256294‑3256592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: