Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,256,450 C→T 8.9% V68I (GTT→ATT)  yhaM ← putative L‑serine dehydratase alpha chain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,256,4500CT 8.9% 399.5 / 17.4 134V68I (GTT→ATT) yhaMputative L‑serine dehydratase alpha chain
Reads supporting (aligned to +/- strand):  ref base C (58/63);  new base T (5/7);  total (64/70)
Fisher's exact test for biased strand distribution p-value = 7.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 137 total aligned reads displayed.

GGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCT  >  NC_000913/3256301‑3256599
                                                                                                                                                     |                                                                                                                                                     
ggAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAAc                                                                                                                                                       >  2:696054/1‑150 (MQ=255)
ggAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCAGGTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGGGCCGCGAAc                                                                                                                                                       >  1:152247/1‑150 (MQ=255)
  cgaCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCGGTTGCGTCTTTCAGCACTTCCAGCCCGGCGGTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGGCACGGGAATgg                                                                                                                                                     <  2:206871/149‑1 (MQ=255)
  agaCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACgg                                                                                                                                                     <  1:1398994/150‑1 (MQ=255)
   gaCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGt                                                                                                                                                    >  1:94444/1‑150 (MQ=255)
   gaCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGACATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGt                                                                                                                                                    <  1:179399/150‑1 (MQ=255)
    aCTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTGGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCGTTCCCGGCACGGGAATGGTg                                                                                                                                                   <  1:18463/150‑1 (MQ=255)
     cTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTTACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGa                                                                                                                                                  <  2:433273/150‑1 (MQ=255)
      tttCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCCGCCCCACCATTCCCGTGCCGGGAACGGTGAc                                                                                                                                                 >  2:1264312/1‑150 (MQ=255)
      tttCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGAc                                                                                                                                                 >  2:1404452/1‑150 (MQ=255)
       ttCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCGTTCCCGGCACGGGAATGGTgggg                                                                                                                                                <  1:198574/150‑4 (MQ=255)
       ttCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTATCAGCACTTCCAGCCGGGCGTGGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGGCACGGGAATGGTgggg                                                                                                                                                <  1:407376/150‑4 (MQ=255)
           ccgccgCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGCCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCAACAATCCCGTGCCGGGAACGGTGACGCCCa                                                                                                                                            >  1:272623/1‑150 (MQ=255)
                gccAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGTATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCGTTCCCGGCACGGGAATGGTg                                                                                                                                                   <  2:1498005/138‑1 (MQ=255)
                gccAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCg                                                                                                                                       >  2:375904/1‑150 (MQ=255)
                    gcagTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGttct                                                                                                                                   >  2:977870/1‑150 (MQ=255)
                     cagTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCTCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGttctt                                                                                                                                  <  1:375904/150‑1 (MQ=255)
                       gTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTtca                                                                                                                                >  2:597019/1‑150 (MQ=255)
                           tttGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCCGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGGGCCGGGACCGGTGACGCCCAGACCGTTCTGCCTCAg                                                                                                                            >  1:1518824/1‑150 (MQ=255)
                           tttGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGGCGGGAACGGTGACGCCCAGACCGTTCTTCATCAg                                                                                                                            <  1:1713735/150‑1 (MQ=255)
                                cATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGCCCGTGCTTCATCAGATTTg                                                                                                                       >  1:509877/1‑150 (MQ=255)
                                cATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTg                                                                                                                       <  1:274415/150‑1 (MQ=255)
                                 aTCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTgg                                                                                                                      <  2:29900/150‑1 (MQ=255)
                                 aTCGGCAATTGCCGGCGGTGTTGCGTCTTTCAGCACTTCCAGCCCGGGGTTGGAATTTCCACCTAACGCCCCCTGCGCCGCCGCACTCGGCAGCCCCACCATTCCCGTGCGGGGAATGGTGACGCCCAGACCGTTCTTCATCAGATTTgg                                                                                                                      <  2:1637826/150‑1 (MQ=255)
                                  tCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGc                                                                                                                     <  1:953604/150‑1 (MQ=255)
                                  tCGGCAATGGCCTGCGCTGTGGCGGCGTGCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCGAGCGCCGCCGCAATCTGCAGCCCCACCATTCCCGTGCCGTGAACGGTGACGCCCAGACCGTTCTGCATCAGATTTGGc                                                                                                                     <  2:1054266/150‑1 (MQ=255)
                                     gCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGaa                                                                                                                  <  2:1570278/150‑1 (MQ=255)
                                     gCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGaa                                                                                                                  >  2:271445/1‑150 (MQ=255)
                                      cAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTg                                                                                                                       >  2:1683346/1‑144 (MQ=255)
                                      cAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTg                                                                                                                       <  1:1683346/144‑1 (MQ=255)
                                        aTTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGACACAAGCTCCGCCGCAATCGGCAGCCCCACCATTCCCGTGACGGGAACGGTGACGCCCAGAACGTGATTCAGAAGATTTGGCGAATcc                                                                                                               >  1:341444/1‑150 (MQ=255)
                                             cTGCGCTGTGGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGc                                                                                                          <  1:1491365/150‑1 (MQ=255)
                                                cgcTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTc                                                                                                       >  1:1706488/1‑150 (MQ=255)
                                                 gcTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCt                                                                                                      <  2:1106426/150‑1 (MQ=255)
                                                  cTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTa                                                                                                     >  2:798874/1‑150 (MQ=255)
                                                      tGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTGCTACACg                                                                                                 >  1:289540/1‑150 (MQ=255)
                                                      tGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTGCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACg                                                                                                 <  2:487978/150‑1 (MQ=255)
                                                      ggCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACg                                                                                                 <  2:841669/149‑1 (MQ=255)
                                                         gTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTc                                                                                              <  1:1718140/150‑1 (MQ=255)
                                                           cTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGAACGTTCTTCAGCAGATTTGGCGAAACCCAGGCTTCGACACGTTCac                                                                                            >  2:1684377/1‑149 (MQ=255)
                                                            tttCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAAc                                                                                           <  1:696054/150‑1 (MQ=255)
                                                              tCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCGAGCGCCGCCGCAATCGGCAGCCCCAACATTCCCGTGCCGGGAACGTTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCg                                                                                         <  2:390135/150‑1 (MQ=255)
                                                                aGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAACCCCAGGCTTCTACACGTTCCACCggc                                                                                       >  2:1715027/1‑149 (MQ=255)
                                                                aGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGa                                                                                       >  1:101055/1‑150 (MQ=255)
                                                                      tCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGCCGCCCAGACCGTTCTTCCGCAGAGTTGGCGACACCCCGGCTGCTACACGTTGAACCGGTCCTTcc                                                                                 >  1:1067389/1‑150 (MQ=255)
                                                                      tCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTcc                                                                                 <  1:1683888/150‑1 (MQ=255)
                                                                       ccAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCa                                                                                <  1:1136781/150‑1 (MQ=255)
                                                                        cAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAg                                                                               >  2:807550/1‑150 (MQ=255)
                                                                          gCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGtt                                                                             >  2:1205093/1‑150 (MQ=255)
                                                                             cGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTg                                                                          <  1:1123227/150‑1 (MQ=255)
                                                                                cGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCg                                                                       <  2:1306191/150‑1 (MQ=255)
                                                                                 gTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCgc                                                                      <  1:1227104/150‑1 (MQ=255)
                                                                                  ttGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCGTCATCAGATTTGGCGAAACCCAGGCGTCTCCACGTTCACCCGGACCTTCCAGTTCTGCCgcc                                                                     >  1:1485393/1‑149 (MQ=255)
                                                                                   tGGCTTTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCgcag                                                                    <  2:152247/150‑1 (MQ=255)
                                                                                   tGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCgcag                                                                    <  1:798324/150‑1 (MQ=255)
                                                                                     gCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAACCCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCgcagca                                                                  >  1:1014362/1‑150 (MQ=255)
                                                                                      cATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTAGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCaa                                                                 >  2:510527/1‑150 (MQ=255)
                                                                                       aTTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTGCACGTTCAACCGGCCCGTGCAGTTCTGCCgcagcatc                                                                >  1:339168/1‑148 (MQ=255)
                                                                                        tttCCCCCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGGGCCGGGAGCGGTGACCCGCAGACGGGTCGTCATCAGAATTGGCGAAAACCAGACTTCTAACGGTTCAACCGGACATTCTAGTTCGGGCGCTGCAccc                                                               >  2:349507/1‑150 (MQ=255)
                                                                                            cACCTAACGCCCCCATCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAAccgccg                                                           <  1:1259721/150‑1 (MQ=255)
                                                                                            cACCTAACGCCCCCAGCGCCGCCGCAATCGGCTGCCCCACCATTCCCGTGCCGGGAACGGTGACGGCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCTACCTGACCTTCCAGTTCTGCCGCAGCAAccgccg                                                           <  1:1266479/150‑1 (MQ=255)
                                                                                            cACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAAccgccg                                                           <  1:1025968/150‑1 (MQ=255)
                                                                                              ccTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAAccgccgcc                                                         >  2:535346/1‑150 (MQ=255)
                                                                                                tAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCCACCGGACCTTCCAGTTCTGCCGCAGCAACcgccgccgc                                                       >  1:1144733/1‑150 (MQ=255)
                                                                                                tAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGAGTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACcgccgccgc                                                       >  2:1034432/1‑150 (MQ=255)
                                                                                                    gCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGGCGCCCAGACCGTTCGTCATCAGCTTTGGCGAAACCCAGGCTTCTACACGTTCCACCGGACCTTCCAGTTCTGCCGCAGCCACCGCCGCcgccagg                                                   >  1:328359/1‑149 (MQ=255)
                                                                                                    gCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCcgccagc                                                   >  2:333093/1‑150 (MQ=255)
                                                                                                     cccccAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCcgccagcg                                                  >  2:893922/1‑150 (MQ=255)
                                                                                                      ccccAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCcgccagcgc                                                 >  1:747507/1‑150 (MQ=255)
                                                                                                      ccccAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCcgccagcgc                                                 >  1:1062868/1‑150 (MQ=255)
                                                                                                       cccAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCcgccagcgcc                                                >  2:1264026/1‑150 (MQ=255)
                                                                                                          aGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGt                                             <  2:1464542/150‑1 (MQ=255)
                                                                                                           gcgcCGCCGCAATCGGCAGCCCCTCCATTCCCGTGCCGGGAACGGTTACGCCCAGACCGTTCTTCATCAGATTTGGCGATACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCATCAACCGCCGCCGCCAGCGCCAGTg                                            <  2:47243/150‑1 (MQ=255)
                                                                                                            cgccgccgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTGCTTCATCAGATTTGGCGACACCCAGGCTTCTACACGATCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGa                                           <  1:216103/150‑1 (MQ=255)
                                                                                                             gccgccgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGaa                                          <  2:1040072/150‑1 (MQ=255)
                                                                                                               cgccgcAATCGGCCGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCACCCGGACCTTCCAGTTCTGCCGCAGCACCCGCCGCCGCCCGCGCCAGTGCAAt                                        >  2:1514484/1‑150 (MQ=255)
                                                                                                                gccgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCCGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCGTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCACTGAAATc                                       >  1:912666/1‑150 (MQ=255)
                                                                                                                gccgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCCGGCTTCTACCCGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGGGAAATc                                       >  1:1064706/1‑150 (MQ=255)
                                                                                                                gccgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATc                                       <  1:430506/150‑1 (MQ=255)
                                                                                                                gccgcAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATc                                       <  1:798874/150‑1 (MQ=255)
                                                                                                                  cgcAATCGGCAGCCCCACCCTTCCCGTGCCGGGAACTGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAACCCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCTGCAACCGCCGCCGCCAGCGCCAGTGAAATCgg                                     <  1:54374/150‑1 (MQ=255)
                                                                                                                         ggCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCTTTATTTATAATTTTTGGCGAAACACAGTCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTa                              <  2:3710/150‑1 (MQ=255)
                                                                                                                              ccccACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATcc                         >  1:1707412/1‑150 (MQ=255)
                                                                                                                               cccACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCACCCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGACATCGGTTCAGTACATccc                        >  1:132652/1‑150 (MQ=255)
                                                                                                                               cccACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATccc                        <  1:1264312/150‑1 (MQ=255)
                                                                                                                                tcaccgTTCCCGGCACGGGAATGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCCGCAACCGCCGCCGCCAGCGCCAGTGAAATCTGTTCAGTACATccgc                       >  2:149768/2‑148 (MQ=255)
                                                                                                                                 caccaTTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAg                      <  2:1062868/150‑1 (MQ=255)
                                                                                                                                 caccaTTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAg                      <  2:963291/150‑1 (MQ=255)
                                                                                                                                  accgTTCCCGGCACGGGAATGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAgc                     >  2:708185/1‑150 (MQ=255)
                                                                                                                                        cccGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCTGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGACGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGt               <  1:869150/150‑1 (MQ=255)
                                                                                                                                        cccGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGAGAAACCCAGGCTTCTACACGTTCAACCGTACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGt               <  1:694334/150‑1 (MQ=255)
                                                                                                                                         ccGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGtt              <  1:886448/150‑1 (MQ=255)
                                                                                                                                          cGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGttt             >  1:527938/1‑150 (MQ=255)
                                                                                                                                            ggCCGGTAACGGGGACGCCCAGACCGTTCTGCATCAGATTTGCCGAACCCCAGGCTTCTCCCCGTTCAACCGGCCCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTa           <  2:1518824/149‑1 (MQ=255)
                                                                                                                                              ccGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTACt         <  1:639574/150‑1 (MQ=255)
                                                                                                                                                gggAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTACTtc       >  2:940660/1‑150 (MQ=255)
                                                                                                                                                 ggAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGGGAAATCGGTACAGTACATCCCAGCGCCGGTTTTACTtcc      >  1:88000/1‑150 (MQ=255)
                                                                                                                                                   aaCGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCCGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTACTtcctc    >  2:906550/1‑150 (MQ=255)
                                                                                                                                                    aCGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTACTtcctcc   <  1:502344/150‑1 (MQ=255)
                                                                                                                                                     cGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTACTTcctcct  >  1:1561246/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGAGACTTTCCCCGCCGCCAGCAGTGCTTTGGCATCGGCAATTGCCTGCGCTGTTGCGTCTTTCAGCACTTCCAGCCCGGCGTTGGCATTTCCACCTAACGCCCCCAGCGCCGCCGCAATCGGCAGCCCCACCATTCCCGTGCCGGGAACGGTGACGCCCAGACCGTTCTTCATCAGATTTGGCGAAACCCAGGCTTCTACACGTTCAACCGGACCTTCCAGTTCTGCCGCAGCAACCGCCGCCGCCAGCGCCAGTGAAATCGGTTCAGTACATCCCAGCGCCGGTTTTACTTCCTCCT  >  NC_000913/3256301‑3256599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: