Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,258,719 A→C 6.7% S311A (TCA→GCA)  tdcG ← L‑serine dehydratase 3, anaerobic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,258,7190AC 6.7% 297.4 / 6.9 108S311A (TCA→GCA) tdcGL‑serine dehydratase 3, anaerobic
Reads supporting (aligned to +/- strand):  ref base A (49/50);  new base C (4/4);  total (53/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

CCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCG  >  NC_000913/3258571‑3258867
                                                                                                                                                    |                                                                                                                                                    
ccgccCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGAc                                                                                                                                                     <  1:1396335/150‑1 (MQ=255)
    ccAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCt                                                                                                                                                 >  1:740137/1‑150 (MQ=255)
     cAGTAGATCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTc                                                                                                                                                <  2:584287/150‑1 (MQ=255)
      agtagtTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGTCAATTGCCCGCtat                                                                                                                                               <  1:1041450/150‑3 (MQ=255)
      agtagtTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCg                                                                                                                                               <  1:1683553/150‑1 (MQ=255)
       gtagtTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGTCAATTGCCCGCtatt                                                                                                                                              <  1:19623/150‑4 (MQ=255)
        tagtTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGTCAATTGCCCGCtattt                                                                                                                                             <  2:1194587/150‑5 (MQ=255)
         agtTCAGTTAACCCTGCCGCAGCCATTGAACAGGCTACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGttt                                                                                                                                            <  2:837046/150‑1 (MQ=255)
          gtTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGCAAATAGCGGGCAATTGCCCGCTCGTTTa                                                                                                                                           >  2:853736/1‑150 (MQ=255)
          gtTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTa                                                                                                                                           >  2:602117/1‑150 (MQ=255)
          gtTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTa                                                                                                                                           >  2:1287546/1‑150 (MQ=255)
            tCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTAcc                                                                                                                                         <  2:38125/150‑1 (MQ=255)
            tCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTAcc                                                                                                                                         <  1:610066/150‑1 (MQ=255)
             cAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCg                                                                                                                                        <  1:54539/150‑1 (MQ=255)
              aGTTAACCCTACCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCgg                                                                                                                                       >  1:805579/1‑150 (MQ=255)
               gTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGga                                                                                                                                      >  2:1164567/1‑150 (MQ=255)
                  aaCCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGgacga                                                                                                                                   >  2:216066/1‑150 (MQ=255)
                    cccTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGacgacg                                                                                                                                 >  2:957172/1‑150 (MQ=255)
                     ccTGCCGCCGCCATTGAACAGGCCACGCCAATCACCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACgg                                                                                                                                <  2:269715/150‑1 (MQ=255)
                         ccgccgccATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACt                                                                                                                            <  1:1348936/150‑1 (MQ=255)
                         ccgccgccATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACt                                                                                                                            <  2:1218182/150‑1 (MQ=255)
                             cgccATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATc                                                                                                                        <  2:695302/150‑1 (MQ=255)
                             cgccATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATc                                                                                                                        >  1:1040106/1‑150 (MQ=255)
                             cgccATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATc                                                                                                                        <  2:805579/150‑1 (MQ=255)
                              gccATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCg                                                                                                                       <  1:1179443/150‑1 (MQ=255)
                              cccATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCAAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCg                                                                                                                       <  1:1562086/149‑1 (MQ=255)
                               ccATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCt                                                                                                                                                 >  2:1119077/1‑123 (MQ=255)
                               ccATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCt                                                                                                                                                 <  1:1119077/123‑1 (MQ=255)
                                cATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGta                                                                                                                     <  1:130686/150‑1 (MQ=255)
                                  ttGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGtaat                                                                                                                   <  2:776451/150‑1 (MQ=255)
                                       cAGGCCACGCCAATCTCCCCCTGACCGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGcc                                                                                                              <  2:1300740/150‑1 (MQ=255)
                                         ggCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAg                                                                                                            >  2:1450774/1‑150 (MQ=255)
                                            cACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTAc                                                                                                         <  1:111950/150‑1 (MQ=255)
                                            cACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAAATGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTAc                                                                                                         >  1:879246/1‑150 (MQ=255)
                                               gCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGc                                                                                                      >  2:247619/1‑150 (MQ=255)
                                               gCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATACCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGc                                                                                                      >  2:1546977/1‑150 (MQ=255)
                                                ccAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGcc                                                                                                     <  2:1465132/150‑1 (MQ=255)
                                                  aaTCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCgg                                                                                                   <  2:1466352/150‑1 (MQ=255)
                                                     ctcCCCCTGACAGCCGACTTCCGCGCCAGAGATGTAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGaat                                                                                                <  1:1074580/150‑1 (MQ=255)
                                                     ctcCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGaat                                                                                                <  1:1355136/150‑1 (MQ=255)
                                                      tcCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGaata                                                                                               <  1:1342391/150‑1 (MQ=255)
                                                       cccccTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGaataa                                                                                              >  1:1176483/1‑150 (MQ=255)
                                                       cccccTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGaataa                                                                                              >  2:1317906/1‑150 (MQ=255)
                                                          ccTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGc                                                                                           <  1:1495840/150‑1 (MQ=255)
                                                             gACAGCCGACTTCCGCGCCAGAGATGGAGGAGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATAGACCGCTCGTTTACCGGACGTCGGAACTTATCGTAATAAGCAAGTACTGCCGGAATAATGCcgc                                                                                        >  1:1158731/1‑150 (MQ=255)
                                                               cAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGTGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAATCCAGAACTGCCGGAATAATGCcccac                                                                                      >  2:1143538/1‑150 (MQ=255)
                                                                    gACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCAcc                                                                                 >  2:1319133/1‑150 (MQ=255)
                                                                         ccGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTAATGCCGGAATAATGCCGCACGCACCGTTAg                                                                            >  2:1558411/1‑150 (MQ=255)
                                                                          cgcgCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGt                                                                           >  2:1491857/1‑150 (MQ=255)
                                                                           gcgcCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTc                                                                          >  2:1716232/1‑150 (MQ=255)
                                                                            cgcCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCg                                                                         >  1:1235933/1‑150 (MQ=255)
                                                                             gcCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCgg                                                                        <  1:1652632/150‑1 (MQ=255)
                                                                               cAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGCCCGCTCGTTTACCGGACGACGCAACTAATCGTTATAAGCCAGTACTGCCGGAATAATGCCGCACCCACCGTTAGTCGGTg                                                                      >  1:1719148/1‑150 (MQ=255)
                                                                                agagATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGc                                                                     >  1:14378/1‑150 (MQ=255)
                                                                                   gaTGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGt                                                                  >  2:456433/1‑150 (MQ=255)
                                                                                      ggAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTAc                                                               <  1:600876/150‑1 (MQ=255)
                                                                                      ggAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTAc                                                               <  1:1138804/150‑1 (MQ=255)
                                                                                       gAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCGAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTAcc                                                              <  1:281857/150‑1 (MQ=255)
                                                                                       gAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTAcc                                                              <  2:186491/150‑1 (MQ=255)
                                                                                           cGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCAcgc                                                          >  1:550228/1‑150 (MQ=255)
                                                                                             ttCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACgcgc                                                        <  1:957172/150‑1 (MQ=255)
                                                                                                    tataCAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAg                                                 <  2:14378/150‑1 (MQ=255)
                                                                                                       aCAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTg                                              <  1:1164567/150‑1 (MQ=255)
                                                                                                           cgcgcCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGACCTTATCGTAATAAGCCAGTACTGCCGGAAGAATGCCGCACGCCCCGGTAGTCGGTGCCGTTACCCCGCGCCCGCCAGCTGCGtt                                          >  1:1656056/1‑150 (MQ=255)
                                                                                                           cgcgcCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGtt                                          >  2:191731/1‑150 (MQ=255)
                                                                                                            gcgcCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGttt                                         <  1:1287546/150‑1 (MQ=255)
                                                                                                             cgcCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGtttt                                        <  2:874329/150‑1 (MQ=255)
                                                                                                              gcCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTCCCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttc                                       >  1:343254/1‑150 (MQ=255)
                                                                                                               ccAATAGGCACCGCTGCCAGAAAATAGTGGGCAATTGACCGCTCGTTTACCGGACGACGGATCTTATCGTAATTCGCCTGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttct                                      <  1:1143538/150‑1 (MQ=255)
                                                                                                                 aaTAGCCCCCGCTGCCAGAAAATAGCGGGCAATTGTCCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttcttc                                    <  1:1052874/150‑1 (MQ=255)
                                                                                                                 aaTAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttcttc                                    <  1:1546977/150‑1 (MQ=255)
                                                                                                                 aaTAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTttcttc                                    <  2:1040106/150‑1 (MQ=255)
                                                                                                                   tAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCAc                                  <  2:1176483/150‑1 (MQ=255)
                                                                                                                    aGCCCCCGCGGCCAGAAAATAGCGGTCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACt                                 <  2:208888/150‑1 (MQ=255)
                                                                                                                      cccccGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTaa                               >  1:1071670/1‑150 (MQ=255)
                                                                                                                         ccGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCg                            <  1:456433/150‑1 (MQ=255)
                                                                                                                          cgcgGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGc                           >  1:1322598/1‑150 (MQ=255)
                                                                                                                             ggCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAg                        <  1:1319133/150‑1 (MQ=255)
                                                                                                                               ccAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAgcg                      >  2:1461950/1‑150 (MQ=255)
                                                                                                                               ccAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAgcg                      >  2:337056/1‑150 (MQ=255)
                                                                                                                                cAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAgcgc                     >  2:710616/1‑150 (MQ=255)
                                                                                                                                    aacgaGCGGTCAATTGCCCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTAc                 >  1:652028/5‑150 (MQ=255)
                                                                                                                                    aaaTAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGAGTAc                 >  2:171616/1‑150 (MQ=255)
                                                                                                                                     aaTAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGGACa                >  1:254898/1‑150 (MQ=255)
                                                                                                                                       tAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATg              >  2:1073212/1‑150 (MQ=255)
                                                                                                                                       tAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGAAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTATTCGGTGCCGTTACCACGCGCCCGCCCGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACAtt              >  2:309189/1‑149 (MQ=255)
                                                                                                                                        aGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGt             <  1:602117/150‑1 (MQ=255)
                                                                                                                                        aGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGt             <  1:1697394/150‑1 (MQ=255)
                                                                                                                                         gCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGtt            >  1:977839/1‑150 (MQ=255)
                                                                                                                                          cGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTg           <  2:879246/150‑1 (MQ=255)
                                                                                                                                           gggCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGa          <  1:1450774/150‑1 (MQ=255)
                                                                                                                                           gggCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGa          <  1:556722/150‑1 (MQ=255)
                                                                                                                                           gggCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGa          <  1:247619/150‑1 (MQ=255)
                                                                                                                                             gCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGAACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTATCTTCACAAACCGCCAGCGCGTACATGTTGATc        >  2:452638/1‑150 (MQ=255)
                                                                                                                                                aTTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAg     >  2:1597455/1‑150 (MQ=255)
                                                                                                                                                aTTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAg     >  1:240434/1‑150 (MQ=255)
                                                                                                                                                aTTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGGACATGTTGATCCAg     >  2:67802/1‑150 (MQ=255)
                                                                                                                                                  tGACCGCTCGCTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTc   >  1:597841/1‑150 (MQ=255)
                                                                                                                                                   gACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCg  >  1:5632/1‑150 (MQ=255)
                                                                                                                                                   gACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCg  <  1:1317906/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCAATCTCCCCCTGACAGCCGACTTCCGCGCCAGAGATGGAGGCGTTCATTTTATACAGCGCGCCAATAGCCCCCGCGGCCAGAAAATAGCGGGCAATTGACCGCTCGTTTACCGGACGACGGAACTTATCGTAATAAGCCAGTACTGCCGGAATAATGCCGCACGCACCGTTAGTCGGTGCCGTTACCACGCGCCCGCCAGCTGCGTTTTCTTCACTAACCGCCAGCGCGTACATGTTGATCCAGTCG  >  NC_000913/3258571‑3258867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑