Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,270,194 G→T 7.6% intergenic (+592/+22) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,270,1940GT 7.6% 130.9 / 3.3 53intergenic (+592/+22)yhaC/rnpBpentapetide repeats‑related protein/RNase P, M1 RNA component
Reads supporting (aligned to +/- strand):  ref base G (34/15);  new base T (2/2);  total (36/17)
Fisher's exact test for biased strand distribution p-value = 5.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.57e-01

CCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGAACCCCTATTTGGCC  >  NC_000913/3270059‑3270343
                                                                                                                                       |                                                                                                                                                     
ccTCCAAAACCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGGCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCGGGtttttgt                                                                                                                                         <  1:1445117/150‑3 (MQ=11)
 cTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGGCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCGGGtttttgct                                                                                                                                        <  1:439088/150‑4 (MQ=11)
   ccAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTa                                                                                                                                      <  1:1594235/150‑1 (MQ=37)
        cgtaaaaaCCCGCTTCGGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTTCTGTTGGGTATAGAGTCGTGGAAGGTCATTCATGTTGAGGCCCGAGCAAAAGCAAAAACCCGCGGCAGCGGGGTTGTGCGTAACTcg                                                                                                                                 >  1:327563/4‑149 (MQ=2)
           ataaaCCCGCCGTATCGGGTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGt                                                                                                                              <  1:673919/148‑1 (MQ=255)
                         gCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATATTTCTGCACCTACAAAAGCAAAAACCCGCAGCAGCGGGGGGTTACCGAAATGAGGGGAAAATGACCGATa                                                                                                                >  1:258839/1‑150 (MQ=11)
                          cGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAATCATTCATAATTCTTCCACAACAAAAGCAAAAACCCCACGAAAACGGTTTTTAAGTAAATAAGGTGAAACGGACCGATac                                                                                                               >  2:1565698/1‑149 (MQ=11)
                             gTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCTTGGAGAGTCCTTCATGATTATGCACCGACAAAAGCAAAAACCCGGCGAAGAGGGGTATTAAGGAAATCAGGTGAAAGTGTCCCAttatcc                                                                                                            >  2:102564/1‑145 (MQ=2)
                                tttACTTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTAGTCGAAAGGCAATCATAATTCTGACACTCCAAAATCAAAAACCCGGAGAAGAGGGTTGTTTCTTTTATCAGGTGAAACTTACCGATAAGCCggg                                                                                                         >  2:937730/1‑150 (MQ=2)
                                 ttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTTGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGt                                                                                                        <  1:1565698/150‑1 (MQ=255)
                                  tACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTGAGCACCTACAAAAGCAAAAACCCGCCGAAGCGGGGTTTTACGTTAATCAGGTGAAATTGTCCGATAAGCCGGGtt                                                                                                       >  2:161077/1‑150 (MQ=14)
                                              gTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCACCTACAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAAGTAAATCAGGTGCAACTGACCGATAAGACGGGTTCTGTAGTGGTCa                                                                                           >  1:245100/1‑150 (MQ=14)
                                                gAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCAACTTTCTGCCCCAACAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGt                                                                                         >  1:1634056/1‑150 (MQ=255)
                                                   aCTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtcat                                                                                      >  1:493073/1‑150 (MQ=255)
                                                                ccGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATATTTCTGCCCCTACAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAAGTAAATCAGGTGAAACAGACCGATAATCCGGGTTCTGTCGTGGACAGTCATTAATCTATGCCac                                                                         >  2:1177210/1‑149 (MQ=17)
                                                                  gCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCa                                                                       >  2:448977/1‑150 (MQ=255)
                                                                  gCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTACAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCa                                                                       >  1:916557/1‑150 (MQ=255)
                                                                  gCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTAATATTTCTGCCCCTCCAAAAGCAAAAAAACGAAGAAGCGGGTTTTTACTTAAATAATGTGTAAATGACCGATAAGCAGGGTTATTTAGTGGACATTCTTTAATATATTCCAgaa                                                                       >  2:1480281/1‑148 (MQ=9)
                                                                       ctttTGGGTATAGCGTCGTGGACAGTCATTCATCTTTATGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCAACTAGGCCAGCAATCGc                                                                  >  1:1690197/1‑150 (MQ=255)
                                                                         tttGGGTATAGCGTCGTGGGCAGTCATTCATCTTTCGGGCCCTCCAAAATCAAAAACCCGCCGAACCCGGGTTTTACGTAAATCAGGGGAAACTGACCGATAAGCCGGGTTCTGTCGGGGGCAGTCATTAATATACGACAGCactataac                                                                >  2:819623/1‑143 (MQ=17)
                                                                                  aGCGTCGTGGACAGTCATTCATCTTTGTGCCCCTCCAAAATCAAAAACCCTCCGATGCGGGTTTTTACGTATATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCa                                                       <  1:475029/150‑1 (MQ=25)
                                                                                     gtcgtGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAagc                                                    >  1:372827/1‑150 (MQ=255)
                                                                                       cgttttcAGTCATTCTTCTTGCTGCCACTCCAAAACCTAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAagcag                                                  <  1:1202767/144‑1 (MQ=25)
                                                                                            aCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTAc                                             >  2:1456144/1‑150 (MQ=255)
                                                                                                 cattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAgg                                                                                                                               >  1:1502973/1‑63 (MQ=35)
                                                                                                 cattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAgg                                                                                                                               <  2:1502973/63‑1 (MQ=35)
                                                                                                 cattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCggg                                        >  2:227065/1‑150 (MQ=255)
                                                                                                 cattcatCTTTCTGCCCATCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCggg                                        >  2:34724/1‑150 (MQ=255)
                                                                                                  attcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGt                                       >  1:444925/1‑150 (MQ=255)
                                                                                                  attcatCTTTCAGCCGCTCCCACACCTAATCCCCGCCGAAGCGGGTTTTTACTTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGt                                       <  1:1194144/150‑1 (MQ=37)
                                                                                                    ttttatatCTGGCACTCCAAAAGCTTAAACCCGCCGGATCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGGCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTc                                     <  2:400110/143‑1 (MQ=37)
                                                                                                     catCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCgg                                                                                                          <  1:337511/80‑1 (MQ=35)
                                                                                                     catCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCgg                                                                                                          >  2:337511/1‑80 (MQ=35)
                                                                                                       tCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTGACTGGCTCACGCAGCCTACCCGGGTTCAGt                                  >  1:309869/1‑150 (MQ=255)
                                                                                                          ttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGcc                                                                                                            <  1:713439/73‑1 (MQ=34)
                                                                                                          ttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGcc                                                                                                            >  2:713439/1‑73 (MQ=34)
                                                                                                             tGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGc                            >  1:668107/1‑150 (MQ=255)
                                                                                                               ccccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCg                          <  2:493073/150‑1 (MQ=255)
                                                                                                                cccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATATAGGCCAGCAATCGCTAACTCGCTAAAGAAGCCAACCCGGGTTAAGTACGGGACga                         >  2:418553/1‑149 (MQ=25)
                                                                                                                   tCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGc                                                                                                             <  1:1595900/63‑1 (MQ=35)
                                                                                                                   tCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGc                                                                                                             >  2:1595900/1‑63 (MQ=35)
                                                                                                                    ccAAAAGCAATATCCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGGCTACCCGGGTTCAGTACTGGCCGTACCt                     <  1:1451027/150‑1 (MQ=255)
                                                                                                                       aaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTAt                  >  1:1128145/1‑150 (MQ=255)
                                                                                                                        aaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATg                 >  2:583425/1‑150 (MQ=255)
                                                                                                                         cgtaaaaaCCCGCTTCGGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGa                >  2:504426/4‑150 (MQ=39)
                                                                                                                         aGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGa                <  2:1634056/150‑1 (MQ=255)
                                                                                                                          gCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGaa               >  1:1661345/1‑150 (MQ=255)
                                                                                                                           cAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCGCACTGGCTGACGCAGCCTACCCGGGTTCAGTACGGGCCGGACCTTATGacc              >  1:137663/1‑148 (MQ=255)
                                                                                                                            aaaaaCCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGAAcc             >  2:314261/1‑150 (MQ=255)
                                                                                                                            aaaaaCCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGAAcc             <  1:448977/150‑1 (MQ=255)
                                                                                                                             aaaaCCCGCTTCGGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGAAccc            >  2:391708/1‑150 (MQ=255)
                                                                                                                               aaCCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGCAccccg          >  1:662229/1‑149 (MQ=255)
                                                                                                                                 cccGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTTCCCGGGTTCAGTACGGGCCGTACCTTATGAACCCCTAt        >  2:242385/1‑150 (MQ=255)
                                                                                                                                       gAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGAACCCCTATTTGGcc  >  1:1240649/1‑150 (MQ=255)
                                                                                                                                       |                                                                                                                                                     
CCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAAGCAGCCTACCCGGGTTCAGTACGGGCCGTACCTTATGAACCCCTATTTGGCC  >  NC_000913/3270059‑3270343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑