Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,276,890 G→A 11.9% intergenic (+37/‑112) garD → / → prlF D‑galactarate dehydrogenase/antitoxin of the SohA(PrlF)‑YhaV toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,276,8900GA11.9% 296.0 / 23.1 110intergenic (+37/‑112)garD/prlFD‑galactarate dehydrogenase/antitoxin of the SohA(PrlF)‑YhaV toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base G (45/50);  new base A (11/2);  total (57/53)
Fisher's exact test for biased strand distribution p-value = 1.62e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.37e-01

Only 100 of 111 total aligned reads displayed.

GGAAGTTGTTCCACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCA  >  NC_000913/3276741‑3277038
                                                                                                                                                     |                                                                                                                                                    
ggAAGTTGTTCCACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAg                                                                                                                                                      >  1:496450/1‑150 (MQ=255)
  aaGTTGTTCCACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTg                                                                                                                                                    <  2:1403370/150‑1 (MQ=255)
     ttgttCCACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCa                                                                                                                                                 <  1:1446681/150‑1 (MQ=255)
       gttCCACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATg                                                                                                                                               <  1:842728/150‑1 (MQ=255)
         tCCACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGtt                                                                                                                                             <  2:552278/150‑1 (MQ=255)
           cACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCACTAAGGCCATGtttt                                                                                                                                           <  2:1188249/150‑1 (MQ=255)
             cTTTATTCTCGACGTCGCCAGCGGCAAGAAGAAAACCTTCTCGGATCAATGGGGCCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTCATTTCTTTAGCAACGACCCACGCAAAACATGCCCTAATTGCCAAGttttaa                                                                                                                                         >  2:694885/1‑148 (MQ=255)
              tttATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTAt                                                                                                                                        <  2:384117/150‑1 (MQ=255)
              tttATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACATCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTAt                                                                                                                                        <  2:204955/150‑1 (MQ=255)
              tttATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCGGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGATCGTCCCTCGCAAAACATGGCACTAAGGCCATGttttgcg                                                                                                                                        <  1:506492/150‑4 (MQ=255)
              attATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGGTTAACCCGGCTCCGGTGACCTGATTTCGTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCTTGTTTTTAt                                                                                                                                        >  1:193529/2‑150 (MQ=255)
               ttATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTAtt                                                                                                                                       >  1:1097930/1‑150 (MQ=255)
                tATTCTCGCCGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGACCGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTg                                                                                                                                      >  1:127418/1‑150 (MQ=255)
                  ttCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGtt                                                                                                                                    <  2:925373/150‑1 (MQ=255)
                  ttCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGtt                                                                                                                                    <  2:500263/150‑1 (MQ=255)
                   tctcGACGTCGCCAGCGGGAAGAAGAACACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGttt                                                                                                                                   <  1:638205/150‑1 (MQ=255)
                    ctcGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTa                                                                                                                                  >  1:695530/1‑150 (MQ=255)
                    ctcGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACAAGGCCTTAGTGCCATGTTTTTATTGTTTa                                                                                                                                  >  1:1525594/1‑150 (MQ=255)
                     tcGCCGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTaa                                                                                                                                 <  2:127418/150‑1 (MQ=255)
                            cGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATATCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGccccc                                                                                                                          >  2:1517319/1‑150 (MQ=255)
                             gCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCa                                                                                                                         <  1:1227941/150‑1 (MQ=255)
                                 gCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTc                                                                                                                     <  1:635599/150‑1 (MQ=255)
                                 gCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTc                                                                                                                     <  1:514675/150‑1 (MQ=255)
                                   gggAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTcc                                                                                                                    >  1:819048/1‑149 (MQ=255)
                                   gggAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTcc                                                                                                                    <  2:819048/149‑1 (MQ=255)
                                    ggAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGATCTGATTTCTTTTGGTACGTAGCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCAt                                                                                                                  <  2:1226448/150‑1 (MQ=255)
                                    ggAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCAt                                                                                                                  >  2:1318738/1‑150 (MQ=255)
                                       agaagaaAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATtaa                                                                                                               >  1:193733/1‑150 (MQ=255)
                                       agaagaaAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACAGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGGTTAAAGCCCCCCCGTCCATtaa                                                                                                               >  1:999412/1‑150 (MQ=255)
                                       agaagaaAACCTTCTCGGATCAATGGGGGCTGCATAAACAGCTGGCGGTGTTTAACCCGGCCCCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAATCCCACACGTCCATtaa                                                                                                               >  1:819435/1‑150 (MQ=255)
                                        gaagaaTACCTTCTCGGTGCACTGTGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACAGGTGACCTGATTTGTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGACATGTTTTTATTGTTTAAAGCCCCCACGTCCATtaat                                                                                                              <  1:224612/150‑1 (MQ=255)
                                         aagaaAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATtaata                                                                                                             >  1:1068133/1‑150 (MQ=255)
                                          agaaAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATtaataa                                                                                                            >  2:1383491/1‑150 (MQ=255)
                                            aaatCCTTCTCGGATCAATTGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCTGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAAtg                                                                                                          <  2:57498/150‑1 (MQ=255)
                                            aaaaCCTTCTCGGATGAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAAtg                                                                                                          <  1:685297/150‑1 (MQ=255)
                                                ccTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAAtgcatt                                                                                                      >  2:1489677/1‑150 (MQ=255)
                                                ccTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAAtgcatt                                                                                                      >  1:491015/1‑150 (MQ=255)
                                                  ttCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAAtgcatttg                                                                                                    <  1:999454/150‑1 (MQ=255)
                                                   tctcGGATCAATGTGTGCTGCATATCCATCTGGCGGTGTTTAACCCGGAACTGTTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAAtgctaatgc                                                                                                   <  2:1165844/150‑7 (MQ=255)
                                                    ctcGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAAtgcatttgca                                                                                                  <  2:1445427/150‑1 (MQ=255)
                                                      cGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAAtgcatttgcatt                                                                                                >  2:718949/1‑150 (MQ=255)
                                                        gATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTAc                                                                                              >  2:1392535/1‑150 (MQ=255)
                                                        gATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTAc                                                                                              >  2:501382/1‑150 (MQ=255)
                                                           cAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTCAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCtt                                                                                           <  2:1133759/150‑1 (MQ=255)
                                                           cAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCtt                                                                                           <  1:1396067/150‑1 (MQ=255)
                                                               gggggCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGAATTTGCATTACCTTAGtt                                                                                       >  2:618542/1‑150 (MQ=255)
                                                                ggggCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTc                                                                                      >  1:1482717/1‑150 (MQ=255)
                                                                 gggCTGCATAACCAGCTGGCGGTGGTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCa                                                                                     <  2:1565465/150‑1 (MQ=255)
                                                                   gCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAg                                                                                   <  2:819131/150‑1 (MQ=255)
                                                                     tGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCt                                                                                 <  2:1023768/150‑1 (MQ=255)
                                                                     tGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCt                                                                                 <  2:1068133/150‑1 (MQ=255)
                                                                        aTAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTtat                                                                              <  1:402014/150‑1 (MQ=255)
                                                                               gCTGGCGGTGTTTACCCCGGCACCGGTGACCTGATTTCGTGTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAGAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTGAGTTCACGCTTATAATTTgc                                                                       >  1:1068410/1‑149 (MQ=255)
                                                                               gCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGATCGTCCATCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCCCGTCCATTAAAAAAGCATTTGCATTACCTTAGTACAAGCTTATAATTTGa                                                                       >  1:1062720/1‑150 (MQ=255)
                                                                               gCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATATAGCAATTGCATTACCTTAGTTCCAGCGTATAATTAGa                                                                       >  2:1426405/1‑150 (MQ=255)
                                                                               gCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTTCCTTAGTTCAAGCTTATAATTTGa                                                                       >  1:235648/1‑150 (MQ=255)
                                                                               gCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCAACGTCCATAAATAATGCATTAGAATTAACTAAATTCAATCTtatattataa                                                                       >  2:1055473/1‑144 (MQ=255)
                                                                                cTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAAGGTCCCTCGCAAAACATGGCCTTAGTTCCATGTTTTTATTGTTTAAAGCCCCCACTTCCATTTATAATCCATTTGCAATACCTTAGTTCAAGCTTATAATTTgtg                                                                      <  1:43287/150‑3 (MQ=255)
                                                                                cTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAg                                                                      <  1:540058/150‑1 (MQ=255)
                                                                                 tGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGc                                                                     <  2:605958/150‑1 (MQ=255)
                                                                                 tGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGc                                                                     >  1:1437203/1‑150 (MQ=255)
                                                                                  ggaggTGTTTAACCCGGCACCGGTGACCTTATTACTTTTGGAACGTCCCTGGCAAAAAATTGCCTTAGTGCCATGTTTTTATTGTTAAAAGCGCCCACGTCCATTAATAAAGCATATGCATTACCTTAGTTCAAGCTTATAATTTGAGCa                                                                    <  2:92675/147‑1 (MQ=255)
                                                                                    cGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCACCCACGTCCATTTATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAgc                                                                  >  2:1265548/1‑149 (MQ=255)
                                                                                       tgtTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGaaaa                                                               >  2:1175749/1‑150 (MQ=255)
                                                                                        gtTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAAc                                                              >  1:1141219/1‑150 (MQ=255)
                                                                                        gtTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAAc                                                              <  2:1653549/150‑1 (MQ=255)
                                                                                        gtTTAACCCGGCACCGGTGACCTGATATCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAAc                                                              <  1:1517319/150‑1 (MQ=255)
                                                                                          ttAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAACCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAg                                                            <  1:308877/150‑1 (MQ=255)
                                                                                            aaCCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGc                                                          <  2:496450/150‑1 (MQ=255)
                                                                                             aCCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCt                                                         >  2:587195/1‑150 (MQ=255)
                                                                                              cccGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGGTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTAAAGCTGTTAATTTGAGCAGAAAACAGGATg                                                        >  1:320059/1‑150 (MQ=255)
                                                                                               ccGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGt                                                       >  1:148673/1‑150 (MQ=255)
                                                                                                    aCCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAg                                                  <  2:193733/150‑1 (MQ=255)
                                                                                                     ccGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTTTAATTTGAGCAGAAAACAGGCTGTAAAAgg                                                 >  2:187160/1‑150 (MQ=255)
                                                                                                       ggTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGAc                                               >  2:812575/1‑150 (MQ=255)
                                                                                                        gTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACa                                              <  1:379653/150‑1 (MQ=255)
                                                                                                            ccTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAATCAGGCTGTATAAGGACAGTGa                                          >  2:206265/1‑150 (MQ=255)
                                                                                                                aTTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCa                                      <  2:1068531/150‑1 (MQ=255)
                                                                                                                 tttCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTAAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGCCAGTGAATCAt                                     >  2:835289/1‑150 (MQ=255)
                                                                                                                        tGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCt                              <  1:1392535/150‑1 (MQ=255)
                                                                                                                            aCGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAATCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGATCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATg                          <  2:819435/150‑1 (MQ=255)
                                                                                                                              gTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATgct                        >  2:480910/1‑150 (MQ=255)
                                                                                                                                aacaataaaaaCATGGCACTAAGGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCAGTAGCTTAGTTCAAGCTTATTATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATTCCCGCTTATgctcg                      >  1:879025/8‑150 (MQ=255)
                                                                                                                                  caataaaaaCATGGCACTAAGGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATTATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATgctcgct                    >  2:206939/6‑150 (MQ=255)
                                                                                                                                  caataaaaaCATGGCACTAAGGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATgctcgct                    >  1:1668738/6‑150 (MQ=255)
                                                                                                                                  cTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGTTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGGGAATCATGCCCGCTAATgcacgct                    >  2:57056/1‑150 (MQ=255)
                                                                                                                                    cGCAAAACATGTCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGctct                  <  1:1383491/150‑1 (MQ=255)
                                                                                                                                    ataaaaaCATGGCACTAAGGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGctct                  >  1:1332515/4‑150 (MQ=255)
                                                                                                                                    ataaaaaCATGGCACTAAGGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGctct                  >  2:1216936/4‑150 (MQ=255)
                                                                                                                                     taaaaaCATGGCACTAAGGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGCtctc                 >  2:1172346/3‑150 (MQ=255)
                                                                                                                                     gCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGCtctc                 >  1:1284912/1‑150 (MQ=255)
                                                                                                                                      cAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGCTCTCa                >  2:1353313/1‑150 (MQ=255)
                                                                                                                                       aaaaCATGGCACTAAGGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGATTCATGCCCGCTAATGCTAGCTCTCAc               >  1:207409/1‑150 (MQ=255)
                                                                                                                                       aaaaCATGGCACTAAGGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGCTCTCAc               >  2:1089952/1‑150 (MQ=255)
                                                                                                                                       aaaaCATGGCACTAAGGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCACTAATGCTCGCTCTCAc               >  2:1077699/1‑150 (MQ=255)
                                                                                                                                        aaaCATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGCTCTCACg              >  1:639487/1‑150 (MQ=255)
                                                                                                                                             tGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCACCCACGTCCATTAATAATGCATTTGCATTACATTATTTCAAGCTTATAATTTGAGCAGAAAACAGGATGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGCTCTCACGCTGTa         >  1:969302/1‑150 (MQ=255)
                                                                                                                                                 cTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGCTCTCACGCTGTACTGa     >  1:165945/1‑150 (MQ=255)
                                                                                                                                                   tAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGCTCTCACGCTGTACTGAcc   <  1:1318738/150‑1 (MQ=255)
                                                                                                                                                    aGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCACTCTCACGCTGTACTGACCa  <  1:501382/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GGAAGTTGTTCCACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCA  >  NC_000913/3276741‑3277038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: