Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,276,891 T→G 11.7% intergenic (+38/‑111) garD → / → prlF D‑galactarate dehydrogenase/antitoxin of the SohA(PrlF)‑YhaV toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,276,8910TG11.7% 275.0 / 27.4 110intergenic (+38/‑111)garD/prlFD‑galactarate dehydrogenase/antitoxin of the SohA(PrlF)‑YhaV toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base T (46/51);  new base G (11/2);  total (57/53)
Fisher's exact test for biased strand distribution p-value = 1.63e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.90e-01

Only 100 of 110 total aligned reads displayed.

AAGTTGTTCCACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCA  >  NC_000913/3276743‑3277038
                                                                                                                                                    |                                                                                                                                                   
aaGTTGTTCCACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTg                                                                                                                                                    <  2:1403370/150‑1 (MQ=255)
   ttgttCCACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCa                                                                                                                                                 <  1:1446681/150‑1 (MQ=255)
     gttCCACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATg                                                                                                                                               <  1:842728/150‑1 (MQ=255)
       tCCACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGtt                                                                                                                                             <  2:552278/150‑1 (MQ=255)
         cACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCACTAAGGCCATGtttt                                                                                                                                           <  2:1188249/150‑1 (MQ=255)
           cTTTATTCTCGACGTCGCCAGCGGCAAGAAGAAAACCTTCTCGGATCAATGGGGCCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTCATTTCTTTAGCAACGACCCACGCAAAACATGCCCTAATTGCCAAGttttaa                                                                                                                                         >  2:694885/1‑148 (MQ=255)
            tttATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACATCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTAt                                                                                                                                        <  2:204955/150‑1 (MQ=255)
            tttATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCGGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGATCGTCCCTCGCAAAACATGGCACTAAGGCCATGttttgcg                                                                                                                                        <  1:506492/150‑4 (MQ=255)
            attATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGGTTAACCCGGCTCCGGTGACCTGATTTCGTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCTTGTTTTTAt                                                                                                                                        >  1:193529/2‑150 (MQ=255)
             ttATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTAtt                                                                                                                                       >  1:1097930/1‑150 (MQ=255)
              tATTCTCGCCGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGACCGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTg                                                                                                                                      >  1:127418/1‑150 (MQ=255)
                ttCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGtt                                                                                                                                    <  2:925373/150‑1 (MQ=255)
                ttCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGtt                                                                                                                                    <  2:500263/150‑1 (MQ=255)
                 tctcGACGTCGCCAGCGGGAAGAAGAACACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGttt                                                                                                                                   <  1:638205/150‑1 (MQ=255)
                  ctcGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTa                                                                                                                                  >  1:695530/1‑150 (MQ=255)
                  ctcGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACAAGGCCTTAGTGCCATGTTTTTATTGTTTa                                                                                                                                  >  1:1525594/1‑150 (MQ=255)
                   tcGCCGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTaa                                                                                                                                 <  2:127418/150‑1 (MQ=255)
                     gACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAg                                                                                                                               <  2:1526334/150‑1 (MQ=255)
                          cGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATATCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGccccc                                                                                                                          >  2:1517319/1‑150 (MQ=255)
                           gCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCa                                                                                                                         <  1:1227941/150‑1 (MQ=255)
                               gCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTc                                                                                                                     <  1:514675/150‑1 (MQ=255)
                               gCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTc                                                                                                                     <  1:635599/150‑1 (MQ=255)
                                 gggAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTcc                                                                                                                    >  1:819048/1‑149 (MQ=255)
                                 gggAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTcc                                                                                                                    <  2:819048/149‑1 (MQ=255)
                                  ggAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGATCTGATTTCTTTTGGTACGTAGCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCAt                                                                                                                  <  2:1226448/150‑1 (MQ=255)
                                  ggAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCAt                                                                                                                  >  2:1318738/1‑150 (MQ=255)
                                   gaagaagaaAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCAtt                                                                                                                 <  1:1530914/150‑1 (MQ=255)
                                     agaagaaAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATtaa                                                                                                               >  1:193733/1‑150 (MQ=255)
                                     agaagaaAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACAGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGGTTAAAGCCCCCCCGTCCATtaa                                                                                                               >  1:999412/1‑150 (MQ=255)
                                     agaagaaAACCTTCTCGGATCAATGGGGGCTGCATAAACAGCTGGCGGTGTTTAACCCGGCCCCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAATCCCACACGTCCATtaa                                                                                                               >  1:819435/1‑150 (MQ=255)
                                      gaagaaTACCTTCTCGGTGCACTGTGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACAGGTGACCTGATTTGTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGACATGTTTTTATTGTTTAAAGCCCCCACGTCCATtaat                                                                                                              <  1:224612/150‑1 (MQ=255)
                                       aagaaAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATtaata                                                                                                             >  1:1068133/1‑150 (MQ=255)
                                        agaaAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATtaataa                                                                                                            >  2:1383491/1‑150 (MQ=255)
                                          aaatCCTTCTCGGATCAATTGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCTGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAAtg                                                                                                          <  2:57498/150‑1 (MQ=255)
                                              ccTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAAtgcatt                                                                                                      >  1:491015/1‑150 (MQ=255)
                                              ccTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAAtgcatt                                                                                                      >  2:1489677/1‑150 (MQ=255)
                                                ttCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAAtgcatttg                                                                                                    <  1:999454/150‑1 (MQ=255)
                                                 tctcGGATCAATGTGTGCTGCATATCCATCTGGCGGTGTTTAACCCGGAACTGTTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAAtgctaatgc                                                                                                   <  2:1165844/150‑7 (MQ=255)
                                                    cGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAAtgcatttgcatt                                                                                                >  2:718949/1‑150 (MQ=255)
                                                      gATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTAc                                                                                              >  2:501382/1‑150 (MQ=255)
                                                      gATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTAc                                                                                              >  2:1392535/1‑150 (MQ=255)
                                                       aTCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTAcc                                                                                             <  2:491015/150‑1 (MQ=255)
                                                         cAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTCAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCtt                                                                                           <  2:1133759/150‑1 (MQ=255)
                                                         cAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCtt                                                                                           <  1:1396067/150‑1 (MQ=255)
                                                              ggggCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTc                                                                                      >  1:1482717/1‑150 (MQ=255)
                                                               gggCTGCATAACCAGCTGGCGGTGGTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCa                                                                                     <  2:1565465/150‑1 (MQ=255)
                                                                 gCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAg                                                                                   <  2:819131/150‑1 (MQ=255)
                                                                   tGCTTAACCAGCTGGCGGTGATTAACCCGGCACCGGTGACCTGATTTCTTTTGTAACGTCCCTCGCAAAACATGGCTTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCt                                                                                 <  1:62027/150‑1 (MQ=255)
                                                                   tGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCt                                                                                 <  2:1068133/150‑1 (MQ=255)
                                                                   tGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCt                                                                                 <  2:1023768/150‑1 (MQ=255)
                                                                          ccAGCTGGCGGTGTTTAACCAGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTATTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAAtt                                                                          <  1:348983/150‑1 (MQ=255)
                                                                             gCTGGCGGTGTTTACCCCGGCACCGGTGACCTGATTTCGTGTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAGAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTGAGTTCACGCTTATAATTTgc                                                                       >  1:1068410/1‑149 (MQ=255)
                                                                             gCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGATCGTCCATCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCCCGTCCATTAAAAAAGCATTTGCATTACCTTAGTACAAGCTTATAATTTGa                                                                       >  1:1062720/1‑150 (MQ=255)
                                                                             gCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATATAGCAATTGCATTACCTTAGTTCCAGCGTATAATTAGa                                                                       >  2:1426405/1‑150 (MQ=255)
                                                                             gCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTTCCTTAGTTCAAGCTTATAATTTGa                                                                       >  1:235648/1‑150 (MQ=255)
                                                                              cTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAAGGTCCCTCGCAAAACATGGCCTTAGTTCCATGTTTTTATTGTTTAAAGCCCCCACTTCCATTTATAATCCATTTGCAATACCTTAGTTCAAGCTTATAATTTgtg                                                                      <  1:43287/150‑3 (MQ=255)
                                                                              cTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAg                                                                      <  1:540058/150‑1 (MQ=255)
                                                                               tGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGc                                                                     <  2:605958/150‑1 (MQ=255)
                                                                               tGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGc                                                                     >  1:1437203/1‑150 (MQ=255)
                                                                                ggaggTGTTTAACCCGGCACCGGTGACCTTATTACTTTTGGAACGTCCCTGGCAAAAAATTGCCTTAGTGCCATGTTTTTATTGTTAAAAGCGCCCACGTCCATTAATAAAGCATATGCATTACCTTAGTTCAAGCTTATAATTTGAGCa                                                                    <  2:92675/147‑1 (MQ=255)
                                                                                  cGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCACCCACGTCCATTTATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAgc                                                                  >  2:1265548/1‑149 (MQ=255)
                                                                                     tgtTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGaaaa                                                               >  2:1175749/1‑150 (MQ=255)
                                                                                      gtTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAAc                                                              >  1:1141219/1‑150 (MQ=255)
                                                                                      gtTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAAc                                                              <  2:1653549/150‑1 (MQ=255)
                                                                                      gtTTAACCCGGCACCGGTGACCTGATATCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAAc                                                              <  1:1517319/150‑1 (MQ=255)
                                                                                        ttAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAACCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAg                                                            <  1:308877/150‑1 (MQ=255)
                                                                                          aaCCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGc                                                          <  2:496450/150‑1 (MQ=255)
                                                                                           aCCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCt                                                         >  2:587195/1‑150 (MQ=255)
                                                                                            cccGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTg                                                        <  2:1097930/150‑1 (MQ=255)
                                                                                            cccGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGGTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTAAAGCTGTTAATTTGAGCAGAAAACAGGATg                                                        >  1:320059/1‑150 (MQ=255)
                                                                                             ccGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGt                                                       >  1:148673/1‑150 (MQ=255)
                                                                                                  aCCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAg                                                  <  2:193733/150‑1 (MQ=255)
                                                                                                   ccGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTTTAATTTGAGCAGAAAACAGGCTGTAAAAgg                                                 >  2:187160/1‑150 (MQ=255)
                                                                                                     ggTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGAc                                               >  2:812575/1‑150 (MQ=255)
                                                                                                      gTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACa                                              <  1:379653/150‑1 (MQ=255)
                                                                                                          ccTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAATCAGGCTGTATAAGGACAGTGa                                          >  2:206265/1‑150 (MQ=255)
                                                                                                              aTTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCa                                      <  2:1068531/150‑1 (MQ=255)
                                                                                                               tttCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTAAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGCCAGTGAATCAt                                     >  2:835289/1‑150 (MQ=255)
                                                                                                                   ttttGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGccc                                 >  1:912914/1‑150 (MQ=255)
                                                                                                                      tGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCt                              <  1:1392535/150‑1 (MQ=255)
                                                                                                                          aCGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAATCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGATCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATg                          <  2:819435/150‑1 (MQ=255)
                                                                                                                            gTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATgct                        >  2:480910/1‑150 (MQ=255)
                                                                                                                              aacaataaaaaCATGGCACTAAGGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCAGTAGCTTAGTTCAAGCTTATTATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATTCCCGCTTATgctcg                      >  1:879025/8‑150 (MQ=255)
                                                                                                                                caataaaaaCATGGCACTAAGGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATTATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATgctcgct                    >  2:206939/6‑150 (MQ=255)
                                                                                                                                caataaaaaCATGGCACTAAGGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATgctcgct                    >  1:1668738/6‑150 (MQ=255)
                                                                                                                                cTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGTTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGGGAATCATGCCCGCTAATgcacgct                    >  2:57056/1‑150 (MQ=255)
                                                                                                                                  cGCAAAACATGTCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGctct                  <  1:1383491/150‑1 (MQ=255)
                                                                                                                                  ataaaaaCATGGCACTAAGGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGctct                  >  1:1332515/4‑150 (MQ=255)
                                                                                                                                   taaaaaCATGGCACTAAGGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGCtctc                 >  2:1172346/3‑150 (MQ=255)
                                                                                                                                   gCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGCtctc                 >  1:1284912/1‑150 (MQ=255)
                                                                                                                                    cAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGCTCTCa                >  2:1353313/1‑150 (MQ=255)
                                                                                                                                    aaaaaCATGGCACTAAGGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAATGACAGTGAATCATGCCCGCTAATGCTCGCTCTCa                >  2:674139/2‑150 (MQ=255)
                                                                                                                                     aaaaCATGGCACTAAGGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGATTCATGCCCGCTAATGCTAGCTCTCAc               >  1:207409/1‑150 (MQ=255)
                                                                                                                                     aaaaCATGGCACTAAGGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGCTCTCAc               >  2:1089952/1‑150 (MQ=255)
                                                                                                                                     aaaaCATGGCACTAAGGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGCTCTCAc               >  2:1310236/1‑150 (MQ=255)
                                                                                                                                     aaaaCATGGCACTAAGGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCACTAATGCTCGCTCTCAc               >  2:1077699/1‑150 (MQ=255)
                                                                                                                                      aaaCATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGCTCTCACg              >  1:639487/1‑150 (MQ=255)
                                                                                                                                           tGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCACCCACGTCCATTAATAATGCATTTGCATTACATTATTTCAAGCTTATAATTTGAGCAGAAAACAGGATGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGCTCTCACGCTGTa         >  1:969302/1‑150 (MQ=255)
                                                                                                                                                 tAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGCTCTCACGCTGTACTGAcc   <  1:1318738/150‑1 (MQ=255)
                                                                                                                                                  aGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCACTCTCACGCTGTACTGACCa  <  1:501382/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AAGTTGTTCCACTTTATTCTCGACGTCGCCAGCGGGAAGAAGAAAACCTTCTCGGATCAATGGGGGCTGCATAACCAGCTGGCGGTGTTTAACCCGGCACCGGTGACCTGATTTCTTTTGGAACGTCCCTCGCAAAACATGGCCTTAGTGCCATGTTTTTATTGTTTAAAGCCCCCACGTCCATTAATAATGCATTTGCATTACCTTAGTTCAAGCTTATAATTTGAGCAGAAAACAGGCTGTAAAAGGACAGTGAATCATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCA  >  NC_000913/3276743‑3277038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: