Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,291,110 A→T 5.6% K766I (AAA→ATA)  yraJ → putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,291,1100AT 5.6% 275.0 / 4.7 104K766I (AAA→ATA) yraJputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (47/48);  new base T (3/3);  total (51/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.71e-01

Only 100 of 107 total aligned reads displayed.

CTCGGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATCAACAATG  >  NC_000913/3290961‑3291257
                                                                                                                                                     |                                                                                                                                                   
ctcGGTAGATACTACGCAGCTGCCGGATGACGTCGATCTTGAACAAGCGGGAGAGTTTGTGGTGCCCAAGAGAGGTGGTATGGTAGCGGCGAGTTTCGGGGGCATTTTCGGTTATGGAGTACTTGTTACAGTCAGCGATCGCAACGGTaa                                                                                                                                                     >  2:610453/1‑150 (MQ=255)
ctcGGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTaa                                                                                                                                                     >  2:622186/1‑150 (MQ=255)
 tcGGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTaaa                                                                                                                                                    <  1:556088/150‑1 (MQ=255)
   ggTAGATACTACGCAGCTGCCCGATAACGTCGAGCGTGAGCAAACAGCACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTGGTTACAGGCAGCGATCGCAACGGTAAAcc                                                                                                                                                  >  2:60617/1‑150 (MQ=255)
    gTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCg                                                                                                                                                 >  2:963031/1‑150 (MQ=255)
      aGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGtt                                                                                                                                               >  1:1189327/1‑150 (MQ=255)
         tactacGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGcc                                                                                                                                            <  2:272837/150‑1 (MQ=255)
           ctacGCAGCTGCCCGATAACGGCGATCTTGAACACACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGAAACGGTTTACCGTTGcgat                                                                                                                                          <  2:1067055/150‑4 (MQ=255)
            tacTCAGCTGCCCGATAACATCGATCTTGAACAAACATCACAGTTTGTGGTGCCAAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACTCCAATATCTGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCtt                                                                                                                                         <  1:1449756/150‑1 (MQ=255)
            tacGCAGCTGCCCGATAACATCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCtt                                                                                                                                         <  1:1456423/150‑1 (MQ=255)
             acGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTTTACCGTTGcgatcg                                                                                                                                        <  2:1720850/150‑6 (MQ=255)
             acGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGGTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTTTACCGTTGcgatcg                                                                                                                                        <  1:26135/150‑6 (MQ=255)
              cGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTg                                                                                                                                       <  2:1347022/150‑1 (MQ=255)
                cggCTGCCGGATAACGTCGGGCTTGAACAGACAACAGAGTTTGTGGGGCGCGGGAGAGGGGCAATGGTAGCGGCGCGGTTCACCGCCAATATCGGTTATCGCGTCCGTGGGGCCGTCCGCGATCGCAACGGGATACCGTTGCGGTTTggg                                                                                                                                     >  2:975602/3‑149 (MQ=255)
                cAGCTGCCCGATAACGTCGATCTTGCACAAACAACTCCGTTTGTGGTGCCCAACCGATGTGCAAGGGGCGCGGCGCGTTGCAACGCCAATATCGGTGATCGCGGACTTGGTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGgc                                                                                                                                     <  1:842646/150‑1 (MQ=255)
                cAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGTAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGgc                                                                                                                                     <  2:1318416/150‑1 (MQ=255)
                cAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGgc                                                                                                                                     <  2:50689/150‑1 (MQ=255)
                cAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGgc                                                                                                                                     >  2:2676/1‑150 (MQ=255)
                cAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGgc                                                                                                                                     <  2:992152/150‑1 (MQ=255)
                  gCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGgcgc                                                                                                                                   <  1:1450032/150‑1 (MQ=255)
                   cTGCCCGATAACGTCGATCTTGCACAAACAACACAGTTTGTGGTGCCCAACAGAGGGGCAATGGTAGCGGCGCGTGTCAACGCCAATCTCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGct                                                                                                                                  <  1:836794/150‑1 (MQ=255)
                    tGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGctc                                                                                                                                 <  1:1161009/150‑1 (MQ=255)
                         gATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGcc                                                                                                                            <  1:972067/150‑1 (MQ=255)
                           tAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAg                                                                                                                          <  2:599835/150‑1 (MQ=255)
                            aaCGGCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTCCTGGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCCGc                                                                                                                         >  1:1170973/1‑150 (MQ=255)
                                  gATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCCGCAACgat                                                                                                                   >  2:255548/1‑150 (MQ=255)
                                  gATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACgat                                                                                                                   >  1:735001/1‑150 (MQ=255)
                                   aTCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACgatg                                                                                                                  >  1:774163/1‑150 (MQ=255)
                                           caaacaaCACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGCTCGCAACGGTAATCCGTTGCCCGTTGGCGCTCTGGCGAGCAACGATGATACgggg                                                                                                          >  2:764756/1‑150 (MQ=255)
                                           caaacaaCACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACgggg                                                                                                          >  2:1157705/1‑150 (MQ=255)
                                            aaacaaCACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGc                                                                                                         <  1:33994/150‑1 (MQ=255)
                                             aacaacACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGGCCTTTGGCGCTGTTGCCAGCAACGATGATACGGGGcc                                                                                                        >  2:1443645/1‑149 (MQ=255)
                                                    caGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGt                                                                                                 >  1:473120/1‑150 (MQ=255)
                                                     aGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGGGGACGTGTTACAGTCAGCGATCGCAACGGTACACCGTTGCCCGTTGGCGCGGGTGGCAGGAGCGTTGGTGCGGGGCAGCAAAggg                                                                                                >  2:1408855/1‑148 (MQ=255)
                                                         tgtgGTGCCCAACAGAGGGGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTAtcgt                                                                                            <  1:827301/150‑1 (MQ=255)
                                                          gtggtgCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTAtcgtc                                                                                           <  2:696137/150‑1 (MQ=255)
                                                           tggtgCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTAtcgtcg                                                                                          <  2:1188035/150‑1 (MQ=255)
                                                           tggggCCCAACTGAGGGGCAATTGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTAtcgtcg                                                                                          <  2:827939/150‑1 (MQ=255)
                                                              tgCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATg                                                                                       >  1:1580074/1‑150 (MQ=255)
                                                              tgCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATg                                                                                       >  1:1413753/1‑150 (MQ=255)
                                                               gCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAATGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGa                                                                                      >  1:63579/1‑150 (MQ=255)
                                                               gCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGa                                                                                      >  1:547649/1‑150 (MQ=255)
                                                               gCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGACCTTTGGCGCTATTGCCAGAAACGATGATACGGGGCATAAAAGTATCGTCGATGa                                                                                      >  1:1351658/1‑150 (MQ=255)
                                                                 ccAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTTCTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTGTGGCGCTCTTGCCAGCAACGATGAGACGGGGCAGCAAAGTTGCGTCGaggggg                                                                                    >  2:1090425/1‑145 (MQ=255)
                                                                 ccAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCGTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAgg                                                                                    >  2:561441/1‑150 (MQ=255)
                                                                  cAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAggg                                                                                   >  1:1168921/1‑150 (MQ=255)
                                                                  cAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAggg                                                                                   >  2:1286383/1‑150 (MQ=255)
                                                                    aCAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGggcg                                                                                 <  1:1312530/150‑1 (MQ=255)
                                                                    aCAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGggcg                                                                                 >  1:1077184/1‑150 (MQ=255)
                                                                    aCAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGggcg                                                                                 <  1:656095/150‑1 (MQ=255)
                                                                     cAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGTTACGGGGCAACAAAGTATCGTCGATGAGggcgg                                                                                <  1:1186620/150‑1 (MQ=255)
                                                                      cgagTTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGggcggc                                                                               <  1:1665/149‑1 (MQ=255)
                                                                      agagGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGggcggc                                                                               >  1:601598/1‑150 (MQ=255)
                                                                      agagGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGggcggc                                                                               <  1:1286383/150‑1 (MQ=255)
                                                                        agGTGCAATGGTAGCGGCGCTTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGGCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTGTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCAt                                                                             <  2:787518/150‑1 (MQ=255)
                                                                              aaTGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACtata                                                                       <  2:735001/150‑1 (MQ=255)
                                                                                 ggTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATAtct                                                                    >  1:446581/1‑150 (MQ=255)
                                                                                     gcggcgCGTTTCAACGCCAATATCGGTTATTGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATctctct                                                                >  1:1471992/1‑150 (MQ=255)
                                                                                     gcggcgCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATctctct                                                                <  2:805669/150‑1 (MQ=255)
                                                                                       ggcgCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTgg                                                              <  2:1532127/150‑1 (MQ=255)
                                                                                        gcgcGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAATCCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTggg                                                             <  2:796010/150‑1 (MQ=255)
                                                                                        gcgcGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTggg                                                             >  1:702042/1‑150 (MQ=255)
                                                                                         cgcgTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGa                                                            <  2:138357/150‑1 (MQ=255)
                                                                                         cgcgTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGa                                                            <  2:414940/150‑1 (MQ=255)
                                                                                           cgTTTCAACGCGAATATCGGTTATCGCGTACTTGGTTCAGTCAGCGATCGCAACGGTAAACCGTTGCACTGTGGCGCTGGTGCCAGCAACGATGAGACGGGGAAACATAGTATGGTCGATGAGGGCGGCATACTTTATGTCTCTGGGata                                                          >  2:892446/1‑150 (MQ=255)
                                                                                             tttCAACGCCAATATCGGTTATCGAGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGACAGCAACGATGATACGGGGCAACAAAGTATCGTGGATGAGGGCGGCCTACTATTTCTCTCTGGGatatg                                                        >  2:1227653/1‑149 (MQ=255)
                                                                                               tCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGa                                                      >  1:455240/1‑150 (MQ=255)
                                                                                                cAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATTTCGAg                                                     >  2:508680/1‑150 (MQ=255)
                                                                                                aaaCGACAATATCGGTTATCTCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAATGTATCGTCGATGAGGTCGGCATACTATATCTCTCTGGGATATCGAg                                                     <  2:871432/149‑1 (MQ=255)
                                                                                                 aaCGCCAATATCGGGTATCGCGTACTTGTTACACTCACCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAATGTATCGTCGATGAGGTCGGCATACTATATCTCTCTGGGATATCGAGt                                                    <  1:1336193/150‑1 (MQ=255)
                                                                                                  aCGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTa                                                   <  2:446581/150‑1 (MQ=255)
                                                                                                   cGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTTCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGaaa                                                  >  1:288652/1‑150 (MQ=255)
                                                                                                   cGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTaa                                                  >  1:1034802/1‑150 (MQ=255)
                                                                                                   cGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTaa                                                  >  2:1581484/1‑150 (MQ=255)
                                                                                                       aaTATCGCTTATCGCGTACTTGATACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTCGCGCTCTTGCCAGCAACGATGATACGGCGCAACAAATTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATca                                              <  1:610453/150‑1 (MQ=255)
                                                                                                        atatCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATcac                                             >  1:1001153/1‑150 (MQ=255)
                                                                                                         tatCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCTTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGTGGCAACAAAGTATCGTCGATGAGTGCGGCATACTATATCTCTCTGGTATATCGAGTAAATcaca                                            <  1:508680/150‑1 (MQ=255)
                                                                                                         tatCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGCTACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATcccc                                            >  2:1249596/1‑147 (MQ=255)
                                                                                                             ggTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGc                                        <  2:1168921/150‑1 (MQ=255)
                                                                                                                    gcgTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTg                                 >  1:1186770/1‑150 (MQ=255)
                                                                                                                    gcgTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTg                                 >  1:1221785/1‑150 (MQ=255)
                                                                                                                          ttGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACAGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCt                           <  1:1476934/150‑1 (MQ=255)
                                                                                                                           tGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAGCGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTg                          >  2:215640/1‑150 (MQ=255)
                                                                                                                           tGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTg                          >  1:1091878/1‑150 (MQ=255)
                                                                                                                             ttACACTCACCGATCGCAACGGAAAACCGTTGCCCTTTGGAGCTCTTGCCAGCAACGATGATACTGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTggg                        <  2:288652/150‑1 (MQ=255)
                                                                                                                               aCAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGa                      >  2:1214714/1‑150 (MQ=255)
                                                                                                                                cAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATATCTGTGGGATATCGAGTAAATCACAAAGCTGGAATGTACGCTGGGGaa                     >  1:158338/1‑150 (MQ=255)
                                                                                                                                  gTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAAt                   >  2:755619/1‑150 (MQ=255)
                                                                                                                                  gTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAAt                   >  2:335975/1‑150 (MQ=255)
                                                                                                                                  gTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCATGCCAGCAACGATGATACGGGGCAACAAAGTATAGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTTAATCACAAAGCTGGACTTTACGCTGGGGAAAt                   >  1:222876/1‑150 (MQ=255)
                                                                                                                                    cAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAAtga                 >  1:1260782/1‑148 (MQ=255)
                                                                                                                                     ccaaagggCAACGGTTTACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTTGGATATCGAGTAAATAACAAAGCTGGACTGTACGCAGGGGAAATCAg                >  1:1708964/8‑150 (MQ=255)
                                                                                                                                     aGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAg                <  2:774163/150‑1 (MQ=255)
                                                                                                                                      gCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAgg               <  1:60617/150‑1 (MQ=255)
                                                                                                                                       ccaccGCAACGCTAAACCGCTGCCCTATCGCCCTCCCGCCCGCAACCCGGATCCCGGGCAACAAATTATCCTCGATGAGGCCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGc              <  1:975602/146‑1 (MQ=255)
                                                                                                                                             caccGGTACCCCGTTGCCCCGTGTCGCTCTTGCCAGCACCGATGCAACGGGGCCACCACGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATCTCGAGTCCATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATca        <  2:1170973/147‑1 (MQ=255)
                                                                                                                                                ccgTACACCGGTGCCCCTAGGCGCCCTTGCCCGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATcaaca     <  1:1408855/148‑1 (MQ=255)
                                                                                                                                                cGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATcaaca     <  2:547649/150‑1 (MQ=255)
                                                                                                                                                 ggTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATcaacaa    >  2:1148361/1‑150 (MQ=255)
                                                                                                                                                   tAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATCAACAATg  <  2:1001153/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CTCGGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATCAACAATG  >  NC_000913/3290961‑3291257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: