Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,305,916 A→T 6.0% intergenic (‑99/+55) mtr ← / ← deaD tryptophan transporter of high affinity/ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,305,9160AT 6.0% 277.7 / 6.1 100intergenic (‑99/+55)mtr/deaDtryptophan transporter of high affinity/ATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base A (38/56);  new base T (2/4);  total (40/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.42e-01

Only 100 of 102 total aligned reads displayed.

CCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACC  >  NC_000913/3305771‑3306060
                                                                                                                                                 |                                                                                                                                                
ccgccAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTaaaaa                                                                                                                                              <  2:1569950/150‑1 (MQ=255)
ccgccAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTaaaaa                                                                                                                                              <  1:853110/150‑1 (MQ=255)
    cAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCgg                                                                                                                                          <  2:1100638/150‑1 (MQ=255)
    cAAGCAGCGACGGTGACGTTTGGGTGGTGGTAAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCgg                                                                                                                                          <  1:1034841/150‑1 (MQ=255)
     aaGCAGCGACGGTGTAGTTTTGGTGGTTGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAATATAGTGCGAATGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAATACAGAAAAAAAGCCCCGATGGTAAAAATCggg                                                                                                                                         <  1:1496158/150‑1 (MQ=255)
      agcagcGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCgggg                                                                                                                                        <  1:725499/150‑1 (MQ=255)
       gcagcGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGc                                                                                                                                       <  2:1653318/150‑1 (MQ=255)
       gcagcGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGc                                                                                                                                       <  1:1587535/150‑1 (MQ=255)
        cagcGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATTTTTACCATCGGGGCt                                                                                                                                      <  2:1176472/150‑1 (MQ=255)
        cagcGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATTTTTACCATCGGGGCt                                                                                                                                      <  2:935193/150‑1 (MQ=255)
         agcGTCGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTg                                                                                                                                     <  2:905539/150‑1 (MQ=255)
         agcGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTg                                                                                                                                     <  2:911664/150‑1 (MQ=255)
          gcGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACATGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGt                                                                                                                                    >  2:230612/1‑150 (MQ=255)
          gcGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATTTTTACCATCGGGGCttt                                                                                                                                    >  2:124104/1‑148 (MQ=255)
          gcGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATTTTTACCATCGGGGCttt                                                                                                                                    <  1:124104/150‑3 (MQ=255)
           cGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAATTAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGta                                                                                                                                   >  2:173781/1‑150 (MQ=255)
           cGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAATTAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGta                                                                                                                                   >  2:230056/1‑150 (MQ=255)
             cctgTGACGTTTGGGTGGTGGTTAGTGTTGCCATGTGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTCTGCATTGCACTGTACCAGTACAAGTGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGTGGCTGtata                                                                                                                                 <  2:1002399/147‑1 (MQ=255)
             ccGGTGACGTTTGGGTTGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAATATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGGACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGtata                                                                                                                                 <  2:1008839/149‑1 (MQ=255)
               ggtttcGTTTTGGTGGTTGTTAGTGTTGCCATGAGGGCTTCTATCCAGTGAAATATAGTGCGACTGCGTTGTTATTCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGtatata                                                                                                                               <  1:927535/145‑1 (MQ=255)
               ggTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATTTTTACCATCGGGGCtttttttc                                                                                                                               <  1:891297/150‑8 (MQ=255)
               ggTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGtatata                                                                                                                               >  1:746278/1‑150 (MQ=255)
                gTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTatatat                                                                                                                              >  2:1398844/1‑150 (MQ=255)
                 tGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATAtatt                                                                                                                             <  2:1681562/150‑1 (MQ=255)
                   aCGTTTGGGTGGTGGTTAGGGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATCGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACCGAAAAAAAGCCCCGATGGTAAAACTCGGGGCTGTATAtattat                                                                                                                           <  1:1538640/150‑1 (MQ=255)
                     gTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTAttt                                                                                                                         >  1:883727/1‑150 (MQ=255)
                       ttGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAAAAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTa                                                                                                                       <  2:1051281/150‑1 (MQ=255)
                       ttGGGGGGTGGTTAGTGTTGCCATCAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCTTTGATATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTa                                                                                                                       <  1:866216/150‑1 (MQ=255)
                         gggTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACa                                                                                                                     <  2:786711/150‑1 (MQ=255)
                         gggTGGTGGTTAGGGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACa                                                                                                                     <  2:718113/150‑1 (MQ=255)
                          ggtggtggtTAGTTTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGAGCGACTGCTTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACATAAAAAAAGCCCCGATTGTAAAAATCGGGGCTGTATATATTATTTTACAg                                                                                                                    <  2:48886/150‑1 (MQ=255)
                          ggtggtggtTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAg                                                                                                                    <  2:1293725/150‑1 (MQ=255)
                          ggtggtggtTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAg                                                                                                                    <  2:1011775/150‑1 (MQ=255)
                             ggtggtTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGAtt                                                                                                                 <  2:746278/150‑1 (MQ=255)
                               tggtTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACCCGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTACAGATtgtg                                                                                                              >  1:294336/1‑150 (MQ=255)
                               tggtTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATtgt                                                                                                               >  1:1554337/1‑150 (MQ=255)
                                ggtTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATtgtg                                                                                                              >  2:583922/1‑150 (MQ=255)
                                ggtTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATtgtg                                                                                                              >  1:1174408/1‑150 (MQ=255)
                                  ttAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGtt                                                                                                            <  1:732625/150‑1 (MQ=255)
                                    aGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCg                                                                                                          <  2:852346/150‑1 (MQ=255)
                                     gtgtTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGc                                                                                                         >  1:1464655/1‑150 (MQ=255)
                                            cATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAg                                                                                                  >  1:978804/1‑150 (MQ=255)
                                             aTGAGGGCTTCTCTCCAGTGAAAATTAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGc                                                                                                 <  1:230056/150‑1 (MQ=255)
                                             aTGAGGGCTTCTCTCCAGTGAAAATTAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGc                                                                                                 <  1:173781/150‑1 (MQ=255)
                                              tGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCg                                                                                                >  1:808828/1‑150 (MQ=255)
                                                  ggCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCgatga                                                                                            <  2:1615325/150‑1 (MQ=255)
                                                    cTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTAGCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAACTCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGAtt                                                                                          <  1:456654/150‑1 (MQ=255)
                                                    cTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAATTCGGGGCTGTATATATTATTTTACAGATTGTGTTCTCTGTTCAGCGATGAtt                                                                                          >  1:730732/1‑150 (MQ=255)
                                                      tctctcCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTAc                                                                                        >  1:931548/1‑150 (MQ=255)
                                                       ctctcCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACg                                                                                       <  2:1547651/150‑1 (MQ=255)
                                                        tctcAAGTGAAAAATATTGCGACTGCGTTGTTATGCATTGAACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGc                                                                                      <  1:918075/150‑1 (MQ=255)
                                                         ctcCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCa                                                                                     >  1:267385/1‑150 (MQ=255)
                                                         ctcCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCa                                                                                     <  1:660386/150‑1 (MQ=255)
                                                         ctcCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCa                                                                                     >  2:786586/1‑150 (MQ=255)
                                                           ccAGTTAAAAATAGTGCTACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACATAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATc                                                                                   <  1:1532525/150‑1 (MQ=255)
                                                           ccAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATc                                                                                   <  2:477920/150‑1 (MQ=255)
                                                                taaaaaTAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATcaccac                                                                              <  1:1182241/149‑1 (MQ=255)
                                                                gAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTc                                                                                                           >  2:1005498/1‑121 (MQ=255)
                                                                gAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTc                                                                                                           <  1:1005498/121‑1 (MQ=255)
                                                                    aaTAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGaa                                                                          >  1:834877/1‑150 (MQ=255)
                                                                     aTAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGaaa                                                                         >  1:109149/1‑150 (MQ=255)
                                                                      acgTGCGCCAGCGTTGTTATGCATTGCACTGTACCAGAACACGACAACAAACGCCAGACAAAACGCCCCGATGGGAAAACTCGGGGCTGTATATATTATTTTACAGATTGTTTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAac                                                                        <  2:644553/148‑1 (MQ=255)
                                                                        gTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacga                                                                      <  2:700879/150‑1 (MQ=255)
                                                                           cGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacgacga                                                                   <  2:928487/150‑1 (MQ=255)
                                                                           cGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacgacga                                                                   >  2:318766/1‑150 (MQ=255)
                                                                            gACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAacgacgac                                                                  <  1:859712/150‑1 (MQ=255)
                                                                             aCTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTACGCTGTTCAGCGATGATTACGCATCACCACCGAAacgacgacg                                                                 >  1:319507/1‑150 (MQ=255)
                                                                                gCGGTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGGAAAAATCGGGGCTGTATATATTATTTTACAGATTGAGTTCGCTGTTCAGCGATGAATACGCATCACCACCGAAACGACTACGAcc                                                              >  1:178686/1‑150 (MQ=255)
                                                                                    ttttATGAATTCCACTGAACCTGAACACTTTTACAAAAGACATAAAAAACCCCCCGATGTAAAAAATCGGGGCTGTAAATATTATTTTACAGATTGTGTGCGCTGTTCAGCTATGATAACGCATCACCCCCTAAACGACCACGACCGGTa                                                          <  2:1417581/148‑1 (MQ=255)
                                                                                      ttATGCATTGCACTGTACCAGTACACGAGTACAATAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACTGAGTGTGTTCGCTGGTCAGCGATGATTACGCGTCGCCACCGAAACGCGGACGAGCGGTAgg                                                        >  2:1610108/1‑149 (MQ=255)
                                                                                       tATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGaa                                                       >  1:1423296/1‑150 (MQ=255)
                                                                                          taaTTGAACTTTACCATTAAAAGAGTAAAAAATACATAAAAAAAGCCCCTATGTTAAAAATCGTGGCTGTTTATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTCCGCATCACCACCGAAACGACGACGACCGGTAGAatca                                                    <  2:1405023/148‑1 (MQ=255)
                                                                                          gCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAatca                                                    <  1:583922/150‑1 (MQ=255)
                                                                                           cATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAatcat                                                   >  2:373671/1‑150 (MQ=255)
                                                                                            aTTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAatcatc                                                  <  2:1174408/150‑1 (MQ=255)
                                                                                                 aatGTACAAGTACACGATTAAAAAATACTTAAAATAAGCCCCGATGGTAAAATTCGGGGATTTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCTTCACAACCGAAACGACGACGACCGGTAGAATCATCacgac                                             <  2:808828/148‑1 (MQ=255)
                                                                                                   tGTACCTGTACACGAGTACAAAAGACAGAAAAAAAGCCCCTATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTGCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCAGTAGAATCATCACGAcgc                                           <  2:757989/150‑1 (MQ=255)
                                                                                                     tACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCgg                                         <  2:1661926/150‑1 (MQ=255)
                                                                                                        cAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAgc                                      >  2:1713760/1‑150 (MQ=255)
                                                                                                           tACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGTTGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGcgcg                                   <  1:1398844/150‑1 (MQ=255)
                                                                                                             cacGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGc                                 >  2:53220/1‑150 (MQ=255)
                                                                                                                   tACAAACGTCAGAAAATCAGCCCCTCTGGTAAAAATCGGGGCTGTATATATTATTTTACAGACTGAGATCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTCGAATCATCACGACGCGGAGCGCGGCCTTCac                           <  1:1086798/150‑1 (MQ=255)
                                                                                                                   tACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGTTAGAATCATCACGACGCGGAGCGCGGCCTTCac                           <  2:530376/150‑1 (MQ=255)
                                                                                                                   tACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCac                           <  2:745335/150‑1 (MQ=255)
                                                                                                                    aCAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCacg                          >  2:927406/1‑150 (MQ=255)
                                                                                                                     cAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCacga                         >  1:997712/1‑150 (MQ=255)
                                                                                                                      aaaaGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACAACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCacgac                        >  1:1312821/1‑150 (MQ=255)
                                                                                                                        aaGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGt                      >  1:660680/1‑150 (MQ=255)
                                                                                                                             aGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTcgcc                 <  2:931548/150‑1 (MQ=255)
                                                                                                                             aGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTcgcc                 <  2:267385/150‑1 (MQ=255)
                                                                                                                             aGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCAGCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTcgcc                 <  1:786586/150‑1 (MQ=255)
                                                                                                                              gAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCCTCCCCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGCCGTTcgccg                >  1:1061883/1‑150 (MQ=255)
                                                                                                                              gAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGCTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTcgccg                >  2:1194839/1‑150 (MQ=255)
                                                                                                                               aaaaaaaGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTcgccgc               <  2:883727/150‑1 (MQ=255)
                                                                                                                                   aaaGCCCCGATTGTAATAATTGTGGTTTTATATATTATTTTACATTTTGTGTTCGCTGTTCAGCGATTATTACGCATCACCACCGAAACGACGACGACCGGTAGATTCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTaaa           <  2:1464655/150‑1 (MQ=255)
                                                                                                                                    caGCCCCGATTTTTACCATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTaaaa          >  2:702331/2‑150 (MQ=255)
                                                                                                                                    aaGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTaaaa          >  2:1252084/1‑150 (MQ=255)
                                                                                                                                     aGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAac         >  1:1647095/1‑150 (MQ=255)
                                                                                                                                     aGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAac         >  2:1651998/1‑150 (MQ=255)
                                                                                                                                            aTGGTAAAAATCGGGGCTTTATATATTATTTTACAGATTTTGTTCGATGTTCAGCTATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGAcc  <  2:1312821/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CCGCCAAGCAGCGACGGTGACGTTTGGGTGGTGGTTAGTGTTGCCATGAGGGCTTCTCTCCAGTGAAAAATAGTGCGACTGCGTTGTTATGCATTGCACTGTACCAGTACACGAGTACAAAAGACAGAAAAAAAGCCCCGATGGTAAAAATCGGGGCTGTATATATTATTTTACAGATTGTGTTCGCTGTTCAGCGATGATTACGCATCACCACCGAAACGACGACGACCGGTAGAATCATCACGACGCGGAGCGCGGCCTTCACGACGTTCGCCGCTAAAACGACGACC  >  NC_000913/3305771‑3306060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: