Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,324,956 C→T 5.5% intergenic (‑45/+45) folP ← / ← ftsH 7,8‑dihydropteroate synthase/protease, ATP‑dependent zinc‑metallo

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,324,9560CT 5.5% 405.8 / 7.1 126intergenic (‑45/+45)folP/ftsH7,8‑dihydropteroate synthase/protease, ATP‑dependent zinc‑metallo
Reads supporting (aligned to +/- strand):  ref base C (58/61);  new base T (4/3);  total (62/64)
Fisher's exact test for biased strand distribution p-value = 7.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 127 total aligned reads displayed.

TGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTT  >  NC_000913/3324811‑3325105
                                                                                                                                                 |                                                                                                                                                     
tGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGccc                                                                                                                                                   >  2:103387/1‑150 (MQ=255)
    tGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCggg                                                                                                                                               <  1:1058071/150‑1 (MQ=255)
     gCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCgggg                                                                                                                                              <  2:1266503/150‑1 (MQ=255)
     gCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCgggg                                                                                                                                              <  2:1146144/150‑1 (MQ=255)
      ccaccaTCCGAACAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGt                                                                                                                                             <  2:875658/150‑1 (MQ=255)
      ccaccaTCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGGGCGTGCTCCGGGGt                                                                                                                                             <  2:1496807/150‑1 (MQ=255)
      ccaccaTCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGt                                                                                                                                             <  1:1587955/150‑1 (MQ=255)
        accaTCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGttt                                                                                                                                           <  2:885713/150‑1 (MQ=255)
        accaTCAGAAAAGGAATCAGGCGTGACGTTTAGGATCCCCATTACGAGAGGATGGCTAAGGTCAAGTGAAGTACCCTGGGAAAAGAGTTTCATGATGTTATAACTTGTATGAATTGATAAGAAAAAACCACGGAGCACTCCCCGGGGttt                                                                                                                                           >  1:1398173/1‑150 (MQ=255)
        acaaTCCGAAAAGTAATCAGTCTTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTTGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGttt                                                                                                                                           <  2:1459120/147‑1 (MQ=255)
          caTCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTc                                                                                                                                         <  1:1181812/150‑1 (MQ=255)
            tCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGCAGTACCCTGGGCAAAGAGGTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCgg                                                                                                                                       <  1:1023610/150‑1 (MQ=255)
            tCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCgg                                                                                                                                       <  1:974256/150‑1 (MQ=255)
            tCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCgg                                                                                                                                       >  1:1351845/1‑150 (MQ=255)
             ccGCAAAGCAATCAGGCGTGACGTTGATGATCCCCATTACGTGACGATGGCTAAGGCCCAGTGAAGTACCCTGGGCAAAGATGTTCATGATGTTATCCCTGTTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGt                                                                                                                                      <  2:245501/150‑1 (MQ=255)
             ccGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGGGCGTGCTCCGGGGttttttct                                                                                                                                      <  2:51114/150‑5 (MQ=255)
             ccGAAAAGGAATCAGGCCTGACGTTGAGGATCCCCATTACGTCAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAACGCGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGCGGTTTTCGGt                                                                                                                                      <  1:336240/150‑1 (MQ=255)
                gaaaGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAAGTGATAAGAAATAACCCCGGAGCACGCCCCGGGGTAGTCGGTACa                                                                                                                                   >  1:277082/2‑150 (MQ=255)
                   aGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAAt                                                                                                                                <  1:189053/150‑1 (MQ=255)
                     gAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATAc                                                                                                                              <  1:1375814/150‑1 (MQ=255)
                     gAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGGCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATAc                                                                                                                              <  1:1166736/150‑1 (MQ=255)
                        tCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGTCGAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGCAAAAACCCCGGAGCACGCCCCGGGGTGATCGGGACAAATACAGt                                                                                                                           >  1:258017/1‑150 (MQ=255)
                          aGGCGTGACGTTGAGGATCACCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCa                                                                                                                         <  2:1372973/150‑1 (MQ=255)
                           ggCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCAt                                                                                                                        >  2:757397/1‑150 (MQ=255)
                           ggCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCAt                                                                                                                        >  1:846690/1‑150 (MQ=255)
                            gCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGCTATTAATTTATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGAACAAATAAATTCATc                                                                                                                       >  2:318335/1‑150 (MQ=255)
                              gTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTg                                                                                                                     <  2:1502333/150‑1 (MQ=255)
                                  ggTTGAGGATCCCCATTACGTTAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGc                                                                                                                 <  1:1073689/149‑1 (MQ=255)
                                  cGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGc                                                                                                                 >  2:726712/1‑150 (MQ=255)
                                   gTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCg                                                                                                                <  2:723208/150‑1 (MQ=255)
                                     tGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCggg                                                                                                              <  2:1200885/150‑1 (MQ=255)
                                     tGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCggg                                                                                                              <  1:1183034/150‑1 (MQ=255)
                                       aGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGaa                                                                                                            <  2:846690/150‑1 (MQ=255)
                                        ggATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAac                                                                                                           >  2:1302960/1‑150 (MQ=255)
                                          aTCCCCATTACGTGAGGATGGCTAAGGTCCAGTGTAGTACCCTGGGCAAAGAGTTTCCAGATGTTATAACTGGTATGAATAGATATGAAAAAACCACGGAGCACGCCCCGGGGTATTAGGTACAAAAACAGTCAAGTCAAGCGCGAacta                                                                                                         >  1:111776/1‑149 (MQ=255)
                                          aTCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAactt                                                                                                         >  2:88798/1‑150 (MQ=255)
                                             ccgaTTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAATACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAacttact                                                                                                      <  2:446017/147‑1 (MQ=255)
                                               cATTACGTGAGGATGGCTAAGGTCCAGTCAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGTACTTACTTg                                                                                                    <  1:1021720/150‑1 (MQ=255)
                                                    cGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCg                                                                                                 >  1:1667854/1‑148 (MQ=255)
                                                    cGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCg                                                                                                 <  2:1667854/148‑1 (MQ=255)
                                                    cGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGcc                                                                                               >  1:1602916/1‑150 (MQ=255)
                                                      tGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTa                                                                                             >  1:209005/1‑150 (MQ=255)
                                                         ggATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACt                                                                                          >  2:1567356/1‑150 (MQ=255)
                                                          gATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTg                                                                                         <  1:1237947/150‑1 (MQ=255)
                                                            tGGCTAAGGTCCAGTGAAGTACGCTGGGCAAAGAGTTTCATGATGGTTGCCCTGGTATGATTTGATAAGAAAAAACCCAGGAGAACCACACGGGGTTTTCGGTACAAATCAAGTCTTCTGATGCGGGAACTTACTTGTCGCCTTAcagag                                                                                       >  2:71101/1‑146 (MQ=255)
                                                            tGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGct                                                                                       >  1:423621/1‑150 (MQ=255)
                                                                tAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGa                                                                                   >  1:1307899/1‑150 (MQ=255)
                                                                 aaGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATTGTATCCCTGGTATGAATTGATAAGAAACAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGAc                                                                                  <  1:1466513/150‑1 (MQ=255)
                                                                   ggTCCAGTGAAGTACCCTGGTCAAAGAGTTTCATTATGTTATCCCTGGTATGATTTGATATGAAAAAACCCAGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTTACAt                                                                                <  2:1398173/150‑1 (MQ=255)
                                                                    gTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATg                                                                               >  1:374215/1‑150 (MQ=255)
                                                                       cAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGgtg                                                                            <  2:904797/150‑1 (MQ=255)
                                                                        aGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGgtgt                                                                           <  1:880961/150‑1 (MQ=255)
                                                                        aGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGgtgt                                                                           <  1:1450818/150‑1 (MQ=255)
                                                                        aGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGgtgt                                                                           >  1:1051517/1‑150 (MQ=255)
                                                                        aGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGgtgt                                                                           >  1:1110986/1‑150 (MQ=255)
                                                                               gaCCCTGGGCAAAGAGTTTCATGATGTTATCCCTTGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCgg                                                                    <  1:347876/149‑1 (MQ=255)
                                                                                aCCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCggg                                                                   >  1:711382/1‑150 (MQ=255)
                                                                                aCCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCggg                                                                   <  1:729290/150‑1 (MQ=255)
                                                                                aCCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCggg                                                                   <  1:88798/150‑1 (MQ=255)
                                                                                aCCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCggg                                                                   >  1:1090333/1‑150 (MQ=255)
                                                                                 cccTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGACCTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGt                                                                  >  1:1637210/1‑150 (MQ=255)
                                                                                 cccTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTTCTCTGACATGGTGTTACCCGGGt                                                                  <  1:1090297/150‑1 (MQ=255)
                                                                                   cTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTc                                                                >  2:1427859/1‑150 (MQ=255)
                                                                                     gggCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCgg                                                              <  1:1567356/150‑1 (MQ=255)
                                                                                       gCAAAGAGTTTCATGATGTTATCCCTGGTAGGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCg                                                            <  1:401893/150‑1 (MQ=255)
                                                                                        cAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGt                                                           >  2:1137454/1‑150 (MQ=255)
                                                                                          aaGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTAc                                                         >  2:1003989/1‑150 (MQ=255)
                                                                                              gTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCgg                                                     >  2:596663/1‑150 (MQ=255)
                                                                                               tttCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGt                                                    <  2:415461/150‑1 (MQ=255)
                                                                                                  cATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTtca                                                 <  2:1351845/150‑1 (MQ=255)
                                                                                                   atgatgTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCACCGGGGTTTTCGGTAAAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTtcat                                                >  1:477032/1‑150 (MQ=255)
                                                                                                    tgatgTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTtcatc                                               >  2:246979/1‑150 (MQ=255)
                                                                                                     gatgTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTtcatca                                              >  2:161788/1‑150 (MQ=255)
                                                                                                      atgTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCaa                                             >  1:574831/1‑150 (MQ=255)
                                                                                                       tgTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAAc                                            >  1:678019/1‑150 (MQ=255)
                                                                                                          tATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCgg                                         >  1:1695589/1‑150 (MQ=255)
                                                                                                              ccTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGTCGTACGCGGTTCATCAACCGGACga                                     >  2:1001535/1‑150 (MQ=255)
                                                                                                              ccTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACgc                                     >  1:1553256/1‑149 (MQ=255)
                                                                                                                  gTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACgaggag                                 <  1:1011972/150‑1 (MQ=255)
                                                                                                                          tGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGa                         <  2:13369/150‑1 (MQ=255)
                                                                                                                          tGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGa                         <  1:757397/150‑1 (MQ=255)
                                                                                                                           gATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGAc                        >  2:255045/1‑150 (MQ=255)
                                                                                                                            aTAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACt                       >  2:1464775/1‑150 (MQ=255)
                                                                                                                               aGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGCGGAGCCTTTGGCCTAcc                    >  1:1078499/1‑150 (MQ=255)
                                                                                                                               aGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTAcc                    <  1:1427859/150‑1 (MQ=255)
                                                                                                                                gAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCa                   <  1:1524050/150‑1 (MQ=255)
                                                                                                                                  gaaaaCCCCGGGGCGTGCTCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACTAtt                 >  1:932792/2‑150 (MQ=255)
                                                                                                                                     aaCCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTc              <  2:1602916/150‑1 (MQ=255)
                                                                                                                                      aCCCCGGGGCGTGCTCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCg             >  1:228925/1‑150 (MQ=255)
                                                                                                                                      aCCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCg             <  1:198458/150‑1 (MQ=255)
                                                                                                                                      aCCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCg             >  1:1320491/1‑150 (MQ=255)
                                                                                                                                       ccccGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGAAGCGGGAACTTACTTGTCGCCTAACTGCTATGACATGGTGTTACCCGGGTACGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCAATGACGc            >  2:351129/1‑150 (MQ=255)
                                                                                                                                        cccGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGcc           >  2:605634/1‑150 (MQ=255)
                                                                                                                                        cccGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGcc           >  1:1432187/1‑150 (MQ=255)
                                                                                                                                        cccGGAGCACGACCCGGGGTTTTCGTTACAAATACAGTCATCTGATGAGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGGCTTTGGACTACCATTGTCGcc           <  2:954720/150‑1 (MQ=255)
                                                                                                                                              gCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAAtt     >  1:1616297/1‑150 (MQ=255)
                                                                                                                                              gCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAAtt     >  2:90927/1‑150 (MQ=255)
                                                                                                                                               cACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTg    >  2:1392653/1‑150 (MQ=255)
                                                                                                                                                aCGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGt   >  1:269797/1‑150 (MQ=255)
                                                                                                                                                 cGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGtt  >  1:1021195/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
TGCGTGCCACCATCCGAAAAGGAATCAGGCGTGACGTTGAGGATCCCCATTACGTGAGGATGGCTAAGGTCCAGTGAAGTACCCTGGGCAAAGAGTTTCATGATGTTATCCCTGGTATGAATTGATAAGAAAAAACCCCGGAGCACGCCCCGGGGTTTTCGGTACAAATACAGTCATCTGATGCGGGAACTTACTTGTCGCCTAACTGCTCTGACATGGTGTTACCCGGGTTCGGCGTACGCGGTTCATCAACCGGACGAGGAGCCTTTGGACTACCATTGTCGCCAGAATTGTT  >  NC_000913/3324811‑3325105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: